Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= NV060500.seq
(503 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At4g18060.1 68417.m02687 SH3 domain-containing protein 3 (SH3P3)... 34 0.047
At1g48970.1 68414.m05489 eukaryotic translation initiation facto... 29 1.8
At2g44350.2 68415.m05517 citrate synthase, mitochondrial, putati... 29 2.4
At2g44350.1 68415.m05516 citrate synthase, mitochondrial, putati... 29 2.4
At5g16220.1 68418.m01895 octicosapeptide/Phox/Bem1p (PB1) domain... 28 3.1
At5g21170.1 68418.m02530 5'-AMP-activated protein kinase beta-2 ... 28 4.1
At5g07920.1 68418.m00916 diacylglycerol kinase 1 (DGK1) identica... 27 5.4
At1g62530.1 68414.m07055 hypothetical protein 27 5.4
At3g60100.1 68416.m06711 citrate synthase, mitochondrial, putati... 27 7.2
At2g21610.1 68415.m02570 pectinesterase family protein contains ... 27 9.5
At2g18900.1 68415.m02205 transducin family protein / WD-40 repea... 27 9.5
>At4g18060.1 68417.m02687 SH3 domain-containing protein 3 (SH3P3)
nearly identical to SH3 domain-containing protein 3
[Arabidopsis thaliana] GI:16974680; contains Pfam
profile PF00018: SH3 domain
Length = 351
Score = 34.3 bits (75), Expect = 0.047
Identities = 15/51 (29%), Positives = 24/51 (47%)
Frame = +1
Query: 304 LVQALYDFTPQEAXELEFRRGDVITVTDRSDQHWWQGXIAHRRGLFPAXYV 456
L + ++ F+ EL+ +GD I V S W +G + G FP Y+
Sbjct: 285 LAEVIHPFSAASEKELDLDKGDYIVVRKVSQTGWAEGECKGKAGWFPMAYI 335
>At1g48970.1 68414.m05489 eukaryotic translation initiation factor
2B family protein / eIF-2B family protein similar to
guanine nucleotide exchange factor, eIF-2B, delta
subunit [Mus musculus] GI:529428; contains Pfam profile
PF01008: Initiation factor 2 subunit family
Length = 756
Score = 29.1 bits (62), Expect = 1.8
Identities = 14/51 (27%), Positives = 25/51 (49%)
Frame = +1
Query: 37 RADAEKLLANKPEGGFLIRISESSPGDFSLSVKCPDGVQHFKVLRDASSKF 189
R+D+ KL A+ P GGF + ++ +P + + Q K + + S F
Sbjct: 140 RSDSTKLSASLPNGGFDLTLAVRAPQESETKIATTSASQGKKKIAEVSETF 190
>At2g44350.2 68415.m05517 citrate synthase, mitochondrial, putative
strong similarity to SP|P20115 Citrate synthase,
mitochondrial precursor {Arabidopsis thaliana};
contains Pfam profile PF00285: Citrate synthase
Length = 474
Score = 28.7 bits (61), Expect = 2.4
Identities = 25/71 (35%), Positives = 29/71 (40%)
Frame = +1
Query: 250 VCSRLQALKLRDVVPIEMLVQALYDFTPQEAXELEFRRGDVITVTDRSDQHWWQGXIAHR 429
VC R ALK P+ LV LY+ P EL G V D H G + +
Sbjct: 365 VCQREFALKHLPDDPLFQLVSKLYEVVPPVLTEL----GKVKNPWPNVDAH--SGVLLNH 418
Query: 430 RGLFPAXYVTV 462
GL A Y TV
Sbjct: 419 YGLTEARYYTV 429
>At2g44350.1 68415.m05516 citrate synthase, mitochondrial, putative
strong similarity to SP|P20115 Citrate synthase,
mitochondrial precursor {Arabidopsis thaliana};
contains Pfam profile PF00285: Citrate synthase
Length = 473
Score = 28.7 bits (61), Expect = 2.4
Identities = 25/71 (35%), Positives = 29/71 (40%)
Frame = +1
Query: 250 VCSRLQALKLRDVVPIEMLVQALYDFTPQEAXELEFRRGDVITVTDRSDQHWWQGXIAHR 429
VC R ALK P+ LV LY+ P EL G V D H G + +
Sbjct: 364 VCQREFALKHLPDDPLFQLVSKLYEVVPPVLTEL----GKVKNPWPNVDAH--SGVLLNH 417
Query: 430 RGLFPAXYVTV 462
GL A Y TV
Sbjct: 418 YGLTEARYYTV 428
>At5g16220.1 68418.m01895 octicosapeptide/Phox/Bem1p (PB1)
domain-containing protein hypothetical proteins -
Arabidopsis thaliana contains Pfam profile PF00564: PB1
domain
Length = 476
Score = 28.3 bits (60), Expect = 3.1
Identities = 22/65 (33%), Positives = 35/65 (53%), Gaps = 9/65 (13%)
Frame = -1
Query: 389 RSVTVMTSPRLNSNSXAS-------CGVKSYRACTNISMGTTSRSLSA*R--RLQTRYDD 237
+SV +M + R NS + S CG +S TN S G+TS S+S+ +++ +D
Sbjct: 197 KSVEMMQTRRTNSGTSGSGDGNGGICGQESMMLETNSSFGSTSSSVSSSNLPPIKSSGED 256
Query: 236 NXASS 222
N A+S
Sbjct: 257 NIANS 261
>At5g21170.1 68418.m02530 5'-AMP-activated protein kinase beta-2
subunit, putative similar to Swiss-Prot:Q9QZH4
5'-AMP-activated protein kinase, beta-2 subunit (AMPK
beta-2 chain) [Rattus norvegicus]
Length = 283
Score = 27.9 bits (59), Expect = 4.1
Identities = 11/30 (36%), Positives = 17/30 (56%)
Frame = +1
Query: 100 ESSPGDFSLSVKCPDGVQHFKVLRDASSKF 189
+ S D S+ P G+ H+KV+ D SK+
Sbjct: 129 QKSGKDHSILFVLPSGIYHYKVIVDGESKY 158
>At5g07920.1 68418.m00916 diacylglycerol kinase 1 (DGK1) identical
to diacylglycerol kinase 1 (Diglyceride kinase 1, DGK 1,
DAG kinase 1) [Arabidopsis thaliana] SWISS-PROT:Q39017
Length = 728
Score = 27.5 bits (58), Expect = 5.4
Identities = 10/25 (40%), Positives = 14/25 (56%)
Frame = +2
Query: 236 YHRTASAVAFKHLNCATSSP*KCWC 310
+HR A H NC++S+P C C
Sbjct: 115 FHRCTICGAAAHFNCSSSAPKDCKC 139
>At1g62530.1 68414.m07055 hypothetical protein
Length = 282
Score = 27.5 bits (58), Expect = 5.4
Identities = 13/38 (34%), Positives = 24/38 (63%), Gaps = 3/38 (7%)
Frame = +1
Query: 262 LQALKLRDVVPIEMLV---QALYDFTPQEAXELEFRRG 366
L L++R+ VP E+ +A+YDF+ ++ ++ RRG
Sbjct: 175 LHTLEIRETVPEELCCVSSKAIYDFSKKKEFGVKLRRG 212
>At3g60100.1 68416.m06711 citrate synthase, mitochondrial, putative
strong similarity to SP|Q43175 Citrate synthase,
mitochondrial precursor {Solanum tuberosum}; contains
Pfam profile PF00285: Citrate synthase
Length = 433
Score = 27.1 bits (57), Expect = 7.2
Identities = 24/71 (33%), Positives = 29/71 (40%)
Frame = +1
Query: 250 VCSRLQALKLRDVVPIEMLVQALYDFTPQEAXELEFRRGDVITVTDRSDQHWWQGXIAHR 429
+C R ALK P+ LV LY+ P EL G V D H G + +
Sbjct: 326 ICQREFALKHLPDDPLFQLVSKLYEVVPPILTEL----GKVKNPWPNVDAH--SGVLLNY 379
Query: 430 RGLFPAXYVTV 462
GL A Y TV
Sbjct: 380 YGLTEARYYTV 390
>At2g21610.1 68415.m02570 pectinesterase family protein contains
Pfam profile: PF01095 pectinesterase
Length = 352
Score = 26.6 bits (56), Expect = 9.5
Identities = 13/39 (33%), Positives = 21/39 (53%)
Frame = +1
Query: 85 LIRISESSPGDFSLSVKCPDGVQHFKVLRDASSKFFLWV 201
LIR+ +S GDFS K + ++ + S +F+WV
Sbjct: 50 LIRVDQSGKGDFS---KIQEAIESIPPNLNNSQLYFIWV 85
>At2g18900.1 68415.m02205 transducin family protein / WD-40 repeat
family protein contains 5 WD-40 repeats (PF00400);
related to LACK protective antigen (GI:13625467)
[Leishmania donovani]
Length = 804
Score = 26.6 bits (56), Expect = 9.5
Identities = 10/20 (50%), Positives = 12/20 (60%)
Frame = +3
Query: 291 PHRNVGAGSIRLHPTRGXRV 350
PHR+ G +I HPTR V
Sbjct: 482 PHRDAGVSAIVFHPTRSMAV 501
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 9,520,101
Number of Sequences: 28952
Number of extensions: 171925
Number of successful extensions: 436
Number of sequences better than 10.0: 11
Number of HSP's better than 10.0 without gapping: 433
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 436
length of database: 12,070,560
effective HSP length: 76
effective length of database: 9,870,208
effective search space used: 898188928
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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