Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= NV060499.seq
(685 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPCC1739.13 |ssa2||heat shock protein Ssa2|Schizosaccharomyces p... 179 4e-46
SPAC13G7.02c |ssa1||heat shock protein Ssa1|Schizosaccharomyces ... 172 3e-44
SPAC22A12.15c |bip1|bip|BiP |Schizosaccharomyces pombe|chr 1|||M... 156 2e-39
SPBC1709.05 |sks2|hsc1|heat shock protein Sks2|Schizosaccharomyc... 149 3e-37
SPAC664.11 |ssc1|ssp1|Hsp70 chaperone mtHsp70|Schizosaccharomyce... 109 5e-25
SPAC110.04c |pss1|ssp1, SPAP14E8.01c|heat shock protein Pss1|Sch... 69 5e-13
SPAC57A7.12 |||heat shock protein Pdr13 |Schizosaccharomyces pom... 53 3e-08
SPAC1F5.06 |||heat shock protein Lhs1 |Schizosaccharomyces pombe... 33 0.051
SPAC25B8.04c |||mitochondrial splicing suppressor |Schizosacchar... 29 0.83
SPCC1322.12c |bub1||serine/threonine protein kinase Bub1|Schizos... 25 7.7
SPAC823.11 |||sphingosine-1-phosphate phosphatase |Schizosacchar... 25 7.7
>SPCC1739.13 |ssa2||heat shock protein Ssa2|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 647
Score = 179 bits (435), Expect = 4e-46
Identities = 104/208 (50%), Positives = 124/208 (59%), Gaps = 4/208 (1%)
Frame = +2
Query: 74 AVGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDTXRLIGDAAKNQVAMNPNN 253
++GIDLGTTYSCVG F + +VEIIANDQGNRTTPSYVAFTDT RLIGDAAKNQVAMNP+N
Sbjct: 4 SIGIDLGTTYSCVGHFSNNRVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVAMNPHN 63
Query: 254 KYSMPNVSSDVSSKMLLCKPT*STGLSRXP----SDGGKPKIKVAYKGEDKTFFPEEVSS 421
++ + + K P + + P S GKP ++V YKGE KTF PEE+SS
Sbjct: 64 --TIFDAKRLIGRK--FDDPEVQSDMKHWPFKVISKDGKPVLQVEYKGETKTFTPEEISS 119
Query: 422 MVLTKMKETAEAYLGKTVQNAVITVPAYFMTLKDKPQKMQVPSQA*TFSESSMNPXXXXX 601
MVL KM+ETAEAYLG V +AV+TVPAYF + + K P
Sbjct: 120 MVLMKMRETAEAYLGGKVTDAVVTVPAYFNDSQRQATKDAGLIAGLNVLRIINEPTAAAI 179
Query: 602 XXXXXXXXXENEMYLSLTSGGGTFDVSI 685
L GGGTFDVS+
Sbjct: 180 AYGLDRSNQGESNVLIFDLGGGTFDVSL 207
Score = 120 bits (289), Expect = 2e-28
Identities = 68/139 (48%), Positives = 79/139 (56%)
Frame = +1
Query: 244 PQQQIFDAKRLIGRKFEDATVQADMKHWXFEXTQ*WRQT*DQGSI*G*RQNLFPRGSQFH 423
P IFDAKRLIGRKF+D VQ+DMKHW F+ + Q G + P
Sbjct: 61 PHNTIFDAKRLIGRKFDDPEVQSDMKHWPFKVISKDGKPVLQVEYKGETKTFTPEEISSM 120
Query: 424 GAYENEGNCRSLSRQNCAECSYHGSRVLHDSQRQATKDAGTISGLNVLRIINEPTAAAIA 603
+ + + +DSQRQATKDAG I+GLNVLRIINEPTAAAIA
Sbjct: 121 VLMKMRETAEAYLGGKVTDAVVTVPAYFNDSQRQATKDAGLIAGLNVLRIINEPTAAAIA 180
Query: 604 YGLDKKGTGERNVLIFDLG 660
YGLD+ GE NVLIFDLG
Sbjct: 181 YGLDRSNQGESNVLIFDLG 199
>SPAC13G7.02c |ssa1||heat shock protein Ssa1|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 644
Score = 172 bits (419), Expect = 3e-44
Identities = 100/208 (48%), Positives = 123/208 (59%), Gaps = 4/208 (1%)
Frame = +2
Query: 74 AVGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDTXRLIGDAAKNQVAMNPNN 253
++GIDLGTTYSCVG F + +VEIIANDQGNRTTPSYVAFTDT RLIGDAAKNQVAMNP+N
Sbjct: 4 SIGIDLGTTYSCVGHFSNNRVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVAMNPHN 63
Query: 254 KYSMPNVSSDVSSKMLLCKPT*STGLSRXP----SDGGKPKIKVAYKGEDKTFFPEEVSS 421
++ + + + P + + P GKP I+V +KGE KTF PEE+SS
Sbjct: 64 --TIFDAKRLIGRR--FNDPEVQSDMKHWPFKVIEKDGKPLIQVEFKGETKTFTPEEISS 119
Query: 422 MVLTKMKETAEAYLGKTVQNAVITVPAYFMTLKDKPQKMQVPSQA*TFSESSMNPXXXXX 601
MVL KM+E+AEA+LG V +AV+TVPAYF + + K P
Sbjct: 120 MVLLKMRESAEAFLGGKVTDAVVTVPAYFNDSQRQATKDAGLIAGLNVLRIINEPTAAAI 179
Query: 602 XXXXXXXXXENEMYLSLTSGGGTFDVSI 685
L GGGTFDVS+
Sbjct: 180 AYGLDRSNQHETNVLIFDLGGGTFDVSL 207
Score = 117 bits (282), Expect = 1e-27
Identities = 66/139 (47%), Positives = 79/139 (56%)
Frame = +1
Query: 244 PQQQIFDAKRLIGRKFEDATVQADMKHWXFEXTQ*WRQT*DQGSI*G*RQNLFPRGSQFH 423
P IFDAKRLIGR+F D VQ+DMKHW F+ + + Q G + P
Sbjct: 61 PHNTIFDAKRLIGRRFNDPEVQSDMKHWPFKVIEKDGKPLIQVEFKGETKTFTPEEISSM 120
Query: 424 GAYENEGNCRSLSRQNCAECSYHGSRVLHDSQRQATKDAGTISGLNVLRIINEPTAAAIA 603
+ + + + +DSQRQATKDAG I+GLNVLRIINEPTAAAIA
Sbjct: 121 VLLKMRESAEAFLGGKVTDAVVTVPAYFNDSQRQATKDAGLIAGLNVLRIINEPTAAAIA 180
Query: 604 YGLDKKGTGERNVLIFDLG 660
YGLD+ E NVLIFDLG
Sbjct: 181 YGLDRSNQHETNVLIFDLG 199
>SPAC22A12.15c |bip1|bip|BiP |Schizosaccharomyces pombe|chr
1|||Manual
Length = 663
Score = 156 bits (379), Expect = 2e-39
Identities = 88/204 (43%), Positives = 117/204 (57%), Gaps = 1/204 (0%)
Frame = +2
Query: 77 VGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDTXRLIGDAAKNQVAMNPNNK 256
+GIDLGTTYSCV V ++G+VEIIANDQGNR TPSYVAFT+ RL+G+AAKNQ NP N
Sbjct: 38 IGIDLGTTYSCVAVMKNGRVEIIANDQGNRITPSYVAFTEDERLVGEAAKNQAPSNPENT 97
Query: 257 -YSMPNVSSDVSSKMLLCKPT*STGLSRXPSDGGKPKIKVAYKGEDKTFFPEEVSSMVLT 433
+ + + + + K S +D +P ++V G+ K F PEE+S+M+L+
Sbjct: 98 IFDIKRLIGRKFDEKTMAKDIKSFPF-HIVNDKNRPLVEVNVGGKKKKFTPEEISAMILS 156
Query: 434 KMKETAEAYLGKTVQNAVITVPAYFMTLKDKPQKMQVPSQA*TFSESSMNPXXXXXXXXX 613
KMK+TAEAYLGK V +AV+TVPAYF + + K P
Sbjct: 157 KMKQTAEAYLGKPVTHAVVTVPAYFNDAQRQATKDAGTIAGLNVIRIVNEPTAAAIAYGL 216
Query: 614 XXXXXENEMYLSLTSGGGTFDVSI 685
E + + GGGTFDVS+
Sbjct: 217 DKTDTEKHIVV-YDLGGGTFDVSL 239
Score = 101 bits (241), Expect = 1e-22
Identities = 55/139 (39%), Positives = 82/139 (58%)
Frame = +1
Query: 244 PQQQIFDAKRLIGRKFEDATVQADMKHWXFEXTQ*WRQT*DQGSI*G*RQNLFPRGSQFH 423
P+ IFD KRLIGRKF++ T+ D+K + F + + ++ G ++ P
Sbjct: 94 PENTIFDIKRLIGRKFDEKTMAKDIKSFPFHIVNDKNRPLVEVNVGGKKKKFTPEEISAM 153
Query: 424 GAYENEGNCRSLSRQNCAECSYHGSRVLHDSQRQATKDAGTISGLNVLRIINEPTAAAIA 603
+ + + + +D+QRQATKDAGTI+GLNV+RI+NEPTAAAIA
Sbjct: 154 ILSKMKQTAEAYLGKPVTHAVVTVPAYFNDAQRQATKDAGTIAGLNVIRIVNEPTAAAIA 213
Query: 604 YGLDKKGTGERNVLIFDLG 660
YGLDK T E++++++DLG
Sbjct: 214 YGLDKTDT-EKHIVVYDLG 231
>SPBC1709.05 |sks2|hsc1|heat shock protein Sks2|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 613
Score = 149 bits (362), Expect = 3e-37
Identities = 91/208 (43%), Positives = 114/208 (54%), Gaps = 4/208 (1%)
Frame = +2
Query: 74 AVGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDTXRLIGDAAKNQVAMNPNN 253
A+GIDLGTTYSCV V++ VEII NDQG RTTPS+VAFT+T RL+GDAAKNQ AMNP N
Sbjct: 8 AIGIDLGTTYSCVAVWETANVEIIPNDQGARTTPSFVAFTETERLVGDAAKNQAAMNPRN 67
Query: 254 KYSMPNVSSDVSSKMLLCKPT*STGLSRXP----SDGGKPKIKVAYKGEDKTFFPEEVSS 421
++ + + + P + P + G P I+V Y GE K F +E+S+
Sbjct: 68 --TVFDAKRLIGRRYE--DPETQKDIKHWPFKVIDNNGIPTIEVNYLGEKKQFTAQEISA 123
Query: 422 MVLTKMKETAEAYLGKTVQNAVITVPAYFMTLKDKPQKMQVPSQA*TFSESSMNPXXXXX 601
MVLTKMKE +EA L K V+ AVITVPAYF + K P
Sbjct: 124 MVLTKMKEISEAKLNKRVEKAVITVPAYFSDSQRAATKDAGAIAGLNVLRIINEPTAAAI 183
Query: 602 XXXXXXXXXENEMYLSLTSGGGTFDVSI 685
+ + L GGGTFDVS+
Sbjct: 184 AYGLDAKSDKPKNVLIFDLGGGTFDVSL 211
Score = 105 bits (253), Expect = 4e-24
Identities = 65/146 (44%), Positives = 80/146 (54%), Gaps = 7/146 (4%)
Frame = +1
Query: 244 PQQQIFDAKRLIGRKFEDATVQADMKHWXFEXTQ*WRQT*DQGSI*G*RQNLFPRGSQFH 423
P+ +FDAKRLIGR++ED Q D+KHW F+ D I N QF
Sbjct: 65 PRNTVFDAKRLIGRRYEDPETQKDIKHWPFKVI-------DNNGIPTIEVNYLGEKKQFT 117
Query: 424 GAYENEGNCRSLSRQNCAECSYHGSRVL-------HDSQRQATKDAGTISGLNVLRIINE 582
+ + + A+ + + + DSQR ATKDAG I+GLNVLRIINE
Sbjct: 118 AQEISAMVLTKMKEISEAKLNKRVEKAVITVPAYFSDSQRAATKDAGAIAGLNVLRIINE 177
Query: 583 PTAAAIAYGLDKKGTGERNVLIFDLG 660
PTAAAIAYGLD K +NVLIFDLG
Sbjct: 178 PTAAAIAYGLDAKSDKPKNVLIFDLG 203
>SPAC664.11 |ssc1|ssp1|Hsp70 chaperone mtHsp70|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 674
Score = 109 bits (261), Expect = 5e-25
Identities = 61/150 (40%), Positives = 85/150 (56%), Gaps = 2/150 (1%)
Frame = +2
Query: 65 KAPAVGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFT-DTXRLIGDAAKNQVAM 241
K P +GIDLGTT SC+ + + ++IAN +G RTTPS VAFT D RL+G +AK Q +
Sbjct: 49 KGPVIGIDLGTTTSCLAIMEGQTPKVIANAEGTRTTPSVVAFTKDGERLVGVSAKRQAVI 108
Query: 242 NPNNKYSMPNVSSDVSSKMLLCKPT*STGLSRXPSDGGKPKIKVAY-KGEDKTFFPEEVS 418
NP N + K +P + P + A+ + KT+ P ++
Sbjct: 109 NPENTFFATKRLIGRRFK----EPEVQRDIKEVPYKIVEHSNGDAWLEARGKTYSPSQIG 164
Query: 419 SMVLTKMKETAEAYLGKTVQNAVITVPAYF 508
+L+KM+ETA YLGK V+NAV+TVPAYF
Sbjct: 165 GFILSKMRETASTYLGKDVKNAVVTVPAYF 194
Score = 79.0 bits (186), Expect = 6e-16
Identities = 53/141 (37%), Positives = 76/141 (53%), Gaps = 2/141 (1%)
Frame = +1
Query: 244 PQQQIFDAKRLIGRKFEDATVQADMKHWXFEXTQ*WRQT*DQGSI*G*RQNLFPRGSQFH 423
P+ F KRLIGR+F++ VQ D+K ++ + + + + P SQ
Sbjct: 110 PENTFFATKRLIGRRFKEPEVQRDIKEVPYKIVE---HSNGDAWLEARGKTYSP--SQIG 164
Query: 424 GAYENE--GNCRSLSRQNCAECSYHGSRVLHDSQRQATKDAGTISGLNVLRIINEPTAAA 597
G ++ + ++ +DSQRQATK AG I+GLNVLR++NEPTAAA
Sbjct: 165 GFILSKMRETASTYLGKDVKNAVVTVPAYFNDSQRQATKAAGAIAGLNVLRVVNEPTAAA 224
Query: 598 IAYGLDKKGTGERNVLIFDLG 660
+AYGLDKK + V +FDLG
Sbjct: 225 LAYGLDKK--NDAIVAVFDLG 243
>SPAC110.04c |pss1|ssp1, SPAP14E8.01c|heat shock protein
Pss1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 720
Score = 69.3 bits (162), Expect = 5e-13
Identities = 45/155 (29%), Positives = 82/155 (52%), Gaps = 2/155 (1%)
Frame = +2
Query: 62 AKAPAVGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDTXRLIGDAAKNQVAM 241
++ VGID G + + + V ++ +++I N+ NR+TPS V++ + R +G+AAK+ A
Sbjct: 3 SRTNVVGIDFGNSKTVIAVARNRAIDVIVNEVSNRSTPSLVSYGERSRFLGEAAKSAEAS 62
Query: 242 NPNNKY-SMPNVSSDVSSKMLLCKPT*STGLSRXPSD-GGKPKIKVAYKGEDKTFFPEEV 415
N N S+ ++ + K S +S ++ G KV Y E+ F ++
Sbjct: 63 NFRNTVGSLKRLAGRTYDDPEI-KDIESNFISAKLTEVDGFVGAKVQYLNEETAFSNIQL 121
Query: 416 SSMVLTKMKETAEAYLGKTVQNAVITVPAYFMTLK 520
+ TK+K AEA L +V + VI++PA+F ++
Sbjct: 122 IAAYFTKIKAIAEAELIGSVSDVVISIPAWFTDIQ 156
Score = 43.6 bits (98), Expect = 3e-05
Identities = 24/54 (44%), Positives = 32/54 (59%), Gaps = 4/54 (7%)
Frame = +1
Query: 511 DSQRQATKDAGTISGLNVLRIINEPTAAAIAYGLDKKGTGE----RNVLIFDLG 660
D QR+A +A I+GLN LR++N+ AAA+ YG+ K E R V I D G
Sbjct: 154 DIQRRALLEAANIAGLNPLRLMNDNAAAALTYGITKTDLPEPESPRRVAIVDFG 207
>SPAC57A7.12 |||heat shock protein Pdr13 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 566
Score = 53.2 bits (122), Expect = 3e-08
Identities = 41/155 (26%), Positives = 68/155 (43%), Gaps = 5/155 (3%)
Frame = +2
Query: 77 VGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDTXRLIGDAAKNQVAMNPNNK 256
+GI G S + + GK +++AN++GNR PS +++ G A+ Q+ N +N
Sbjct: 27 IGISFGNQNSSIAFNRDGKTDVLANEEGNRQIPSILSYHGDQEYHGVQARGQLVRNADNS 86
Query: 257 Y-SMPNVSSDVSSKMLLCKPT*STGLSRXPSDGGKPKIKVAYKGE----DKTFFPEEVSS 421
+ ++ ++ L S G KI V E +K E S
Sbjct: 87 VTNFRDLLGKSHDELTLHHCHYSANPVNVEGQIGF-KITVQEDEESDPKEKILTAHEASV 145
Query: 422 MVLTKMKETAEAYLGKTVQNAVITVPAYFMTLKDK 526
L ++ E+AE +LG V V++VP YF + K
Sbjct: 146 RHLRRLTESAEDFLGTKVNGCVMSVPVYFTDAQRK 180
>SPAC1F5.06 |||heat shock protein Lhs1 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 848
Score = 32.7 bits (71), Expect = 0.051
Identities = 30/151 (19%), Positives = 65/151 (43%), Gaps = 3/151 (1%)
Frame = +2
Query: 77 VGIDLGTTYSCVGVFQHG-KVEIIANDQGNRTTPSYVAFTDTXRLIGDAAKNQVAMNPNN 253
+ ID GT ++ + + G +EI+ R S VAF R+ G A N P +
Sbjct: 25 LAIDYGTEWTKAALIKPGIPLEIVLTKDTRRKEQSAVAFKGNERIFGVDASNLATRFPAH 84
Query: 254 KY-SMPNVSSDVSSKMLLCKPT*STGLSRXPSDGGKPKIKVAYK-GEDKTFFPEEVSSMV 427
++ + + +L + S+ + + + +++ +++ + EE+ +M
Sbjct: 85 SIRNVKELLDTAGLESVLVQKYQSSYPAIQLVENEETTSGISFVISDEENYSLEEIIAMT 144
Query: 428 LTKMKETAEAYLGKTVQNAVITVPAYFMTLK 520
+ AE + + + V+TVP +F L+
Sbjct: 145 MEHYISLAEEMAHEKITDLVLTVPPHFNELQ 175
>SPAC25B8.04c |||mitochondrial splicing suppressor
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 378
Score = 28.7 bits (61), Expect = 0.83
Identities = 12/32 (37%), Positives = 18/32 (56%)
Frame = +3
Query: 69 HPQ*ESIWVPRTLALVSSSTGRWRSSPTTRAT 164
HP S W+P LVS+ W +SP+++ T
Sbjct: 283 HPLYSSSWIPTLHDLVSTRCSVWLTSPSSQRT 314
>SPCC1322.12c |bub1||serine/threonine protein kinase
Bub1|Schizosaccharomyces pombe|chr 3|||Manual
Length = 1044
Score = 25.4 bits (53), Expect = 7.7
Identities = 16/56 (28%), Positives = 28/56 (50%), Gaps = 2/56 (3%)
Frame = -1
Query: 415 DFLGEKGFVFTLIC--YLDLRFASITGXPRKXSASCRLAQ*HLRTYVR*DVWHRIF 254
D+ G ++T++ Y++ R I G R+ L Q ++ Y D+WHR+F
Sbjct: 941 DYHGLAAIIYTMLFGQYIETRIEVINGQRRQV-----LTQ-RMKRYWNQDLWHRLF 990
>SPAC823.11 |||sphingosine-1-phosphate phosphatase
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 411
Score = 25.4 bits (53), Expect = 7.7
Identities = 11/35 (31%), Positives = 19/35 (54%)
Frame = -3
Query: 155 GRWR*SPPSRAGRHQRKSTWYPDRFLLRVLLPFCI 51
G W SP S + H ++++ +F +RVL C+
Sbjct: 276 GTWFASPESLSHLHDNLNSYFLLKFFVRVLFGVCM 310
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,866,063
Number of Sequences: 5004
Number of extensions: 58265
Number of successful extensions: 230
Number of sequences better than 10.0: 11
Number of HSP's better than 10.0 without gapping: 202
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 223
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 315915086
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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