Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= NV060497.seq
(685 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPCC794.09c |ef1a-a||translation elongation factor EF-1 alpha Ef... 157 1e-39
SPAC23A1.10 |ef1a-b||translation elongation factor EF-1 alpha Ef... 157 1e-39
SPBC839.15c |ef1a-c||translation elongation factor EF-1 alpha Ef... 157 1e-39
SPCC584.04 |sup35|erf3|translation release factor eRF3 |Schizosa... 91 2e-19
SPBC25B2.01 ||SPBC2G5.08|elongation factor 1 alpha related prote... 89 6e-19
SPBC9B6.04c |tuf1||mitochondrial translation elongation factor E... 70 4e-13
SPCP31B10.07 |eft202||translation elongation factor 2 |Schizosac... 36 0.007
SPAC513.01c |eft201|eft2-1, etf2, SPAPYUK71.04c|translation elon... 36 0.007
SPBC660.10 |||translation elongation factor G|Schizosaccharomyce... 35 0.013
SPBC1306.01c ||SPBC409.22c|translation elongation factor G|Schiz... 34 0.017
SPBC1271.15c |||translation initiation factor IF-2Mt|Schizosacch... 33 0.051
SPBC17G9.09 |tif213||translation initiation factor eIF2 gamma su... 32 0.067
SPAC1B3.04c |||mitochondrial GTPase Guf1 |Schizosaccharomyces po... 30 0.27
SPCC553.08c |||GTPase Ria1 |Schizosaccharomyces pombe|chr 3|||Ma... 30 0.27
SPAC56F8.03 |||translation initiation factor IF2 |Schizosaccharo... 29 0.47
SPCC364.02c |bis1||stress response protein Bis1|Schizosaccharomy... 28 1.1
SPBC215.12 |cwf10|spef2, snu114|GTPase Cwf10 |Schizosaccharomyce... 27 3.3
SPBC1703.06 |pof10||F-box protein Pof10|Schizosaccharomyces pomb... 25 7.7
>SPCC794.09c |ef1a-a||translation elongation factor EF-1 alpha
Ef1a-a |Schizosaccharomyces pombe|chr 3|||Manual
Length = 460
Score = 157 bits (382), Expect = 1e-39
Identities = 79/119 (66%), Positives = 90/119 (75%)
Frame = +2
Query: 161 QTYHREVREGGPGNG*RILQICLGIGQTKG*GELGITIDIALWKFXTSKYYVTIIDAPGH 340
+ + +E E G G+ + + + K E GITIDIALWKF T KY VT+IDAPGH
Sbjct: 40 EKFEKEATELGKGS----FKYAWVLDKLKAERERGITIDIALWKFETPKYNVTVIDAPGH 95
Query: 341 RDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNGQTREHALLAFTLGVKQLIVGXTK 517
RDFIKNMITGTSQADCAVLI+ GTGEFEAGISK+GQTREHALLA+TLGVKQLIV K
Sbjct: 96 RDFIKNMITGTSQADCAVLIIGGGTGEFEAGISKDGQTREHALLAYTLGVKQLIVAVNK 154
Score = 137 bits (331), Expect = 2e-33
Identities = 62/66 (93%), Positives = 64/66 (96%)
Frame = +3
Query: 57 MGKEKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVL 236
MGKEK HIN+VVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEA E+GKGSFKYAWVL
Sbjct: 1 MGKEKGHINVVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEATELGKGSFKYAWVL 60
Query: 237 DKLKAE 254
DKLKAE
Sbjct: 61 DKLKAE 66
>SPAC23A1.10 |ef1a-b||translation elongation factor EF-1 alpha
Ef1a-b |Schizosaccharomyces pombe|chr 1|||Manual
Length = 460
Score = 157 bits (381), Expect = 1e-39
Identities = 78/119 (65%), Positives = 90/119 (75%)
Frame = +2
Query: 161 QTYHREVREGGPGNG*RILQICLGIGQTKG*GELGITIDIALWKFXTSKYYVTIIDAPGH 340
+ + +E E G G+ + + + K E GITIDIALWKF T KY VT+IDAPGH
Sbjct: 40 EKFEKEATELGKGS----FKYAWVLDKLKAERERGITIDIALWKFETPKYNVTVIDAPGH 95
Query: 341 RDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNGQTREHALLAFTLGVKQLIVGXTK 517
RDFIKNMITGTSQADCA+LI+ GTGEFEAGISK+GQTREHALLA+TLGVKQLIV K
Sbjct: 96 RDFIKNMITGTSQADCAILIIGGGTGEFEAGISKDGQTREHALLAYTLGVKQLIVAVNK 154
Score = 137 bits (331), Expect = 2e-33
Identities = 62/66 (93%), Positives = 64/66 (96%)
Frame = +3
Query: 57 MGKEKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVL 236
MGKEK HIN+VVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEA E+GKGSFKYAWVL
Sbjct: 1 MGKEKGHINVVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEATELGKGSFKYAWVL 60
Query: 237 DKLKAE 254
DKLKAE
Sbjct: 61 DKLKAE 66
>SPBC839.15c |ef1a-c||translation elongation factor EF-1 alpha
Ef1a-c |Schizosaccharomyces pombe|chr 2|||Manual
Length = 460
Score = 157 bits (381), Expect = 1e-39
Identities = 78/119 (65%), Positives = 90/119 (75%)
Frame = +2
Query: 161 QTYHREVREGGPGNG*RILQICLGIGQTKG*GELGITIDIALWKFXTSKYYVTIIDAPGH 340
+ + +E E G G+ + + + K E GITIDIALWKF T KY VT+IDAPGH
Sbjct: 40 EKFEKEATELGKGS----FKYAWVLDKLKAERERGITIDIALWKFETPKYNVTVIDAPGH 95
Query: 341 RDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNGQTREHALLAFTLGVKQLIVGXTK 517
RDFIKNMITGTSQADCA+LI+ GTGEFEAGISK+GQTREHALLA+TLGVKQLIV K
Sbjct: 96 RDFIKNMITGTSQADCAILIIGGGTGEFEAGISKDGQTREHALLAYTLGVKQLIVAVNK 154
Score = 137 bits (331), Expect = 2e-33
Identities = 62/66 (93%), Positives = 64/66 (96%)
Frame = +3
Query: 57 MGKEKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVL 236
MGKEK HIN+VVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEA E+GKGSFKYAWVL
Sbjct: 1 MGKEKGHINVVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEATELGKGSFKYAWVL 60
Query: 237 DKLKAE 254
DKLKAE
Sbjct: 61 DKLKAE 66
>SPCC584.04 |sup35|erf3|translation release factor eRF3
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 662
Score = 90.6 bits (215), Expect = 2e-19
Identities = 41/87 (47%), Positives = 57/87 (65%)
Frame = +2
Query: 257 ELGITIDIALWKFXTSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGI 436
E G T+++ F T +++DAPGH+ ++ NMI G SQAD VL+++A GEFEAG
Sbjct: 299 EKGKTVEVGRAYFETEHRRFSLLDAPGHKGYVTNMINGASQADIGVLVISARRGEFEAGF 358
Query: 437 SKNGQTREHALLAFTLGVKQLIVGXTK 517
+ GQTREHA+LA T G+ L+V K
Sbjct: 359 ERGGQTREHAVLARTQGINHLVVVINK 385
Score = 76.6 bits (180), Expect = 3e-15
Identities = 33/62 (53%), Positives = 45/62 (72%)
Frame = +3
Query: 69 KTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVLDKLK 248
K H+NIV IGHVD+GKST G++++ G +DKRT+EK E+EA+E GK S+ +W LD
Sbjct: 236 KEHVNIVFIGHVDAGKSTLGGNILFLTGMVDKRTMEKIEREAKEAGKESWYLSWALDSTS 295
Query: 249 AE 254
E
Sbjct: 296 EE 297
>SPBC25B2.01 ||SPBC2G5.08|elongation factor 1 alpha related
protein|Schizosaccharomyces pombe|chr 2|||Manual
Length = 592
Score = 89.0 bits (211), Expect = 6e-19
Identities = 42/85 (49%), Positives = 53/85 (62%)
Frame = +2
Query: 263 GITIDIALWKFXTSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISK 442
G+T+D+A F + K I DAPGHRDFI MI G S AD AVL+V + FE G +
Sbjct: 240 GVTMDVASTTFESDKKIYEIGDAPGHRDFISGMIAGASSADFAVLVVDSSQNNFERGFLE 299
Query: 443 NGQTREHALLAFTLGVKQLIVGXTK 517
NGQTREHA L LG+ +++V K
Sbjct: 300 NGQTREHAYLLRALGISEIVVSVNK 324
Score = 70.5 bits (165), Expect = 2e-13
Identities = 30/62 (48%), Positives = 43/62 (69%)
Frame = +3
Query: 69 KTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVLDKLK 248
K +++VV GHVDSGKST G ++++ G I+ R+++K EA GKGSF YAW+LD +
Sbjct: 175 KPVVHLVVTGHVDSGKSTMLGRIMFELGEINSRSMQKLHNEAANSGKGSFSYAWLLDTTE 234
Query: 249 AE 254
E
Sbjct: 235 EE 236
>SPBC9B6.04c |tuf1||mitochondrial translation elongation factor
EF-Tu Tuf1 |Schizosaccharomyces pombe|chr 2|||Manual
Length = 439
Score = 69.7 bits (163), Expect = 4e-13
Identities = 37/85 (43%), Positives = 52/85 (61%)
Frame = +2
Query: 263 GITIDIALWKFXTSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISK 442
GITI A ++ T+ + +D PGH D+IKNMITG + D A+++V+A G+
Sbjct: 101 GITISSAHVEYETANRHYAHVDCPGHADYIKNMITGAATMDGAIIVVSATDGQMP----- 155
Query: 443 NGQTREHALLAFTLGVKQLIVGXTK 517
QTREH LLA +GVKQ++V K
Sbjct: 156 --QTREHLLLARQVGVKQIVVYINK 178
Score = 35.1 bits (77), Expect = 0.010
Identities = 16/29 (55%), Positives = 20/29 (68%)
Frame = +3
Query: 63 KEKTHINIVVIGHVDSGKSTTTGHLIYKC 149
++K H+NI IGHVD GK+T T I KC
Sbjct: 49 RKKPHVNIGTIGHVDHGKTTLTA-AITKC 76
>SPCP31B10.07 |eft202||translation elongation factor 2
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 842
Score = 35.5 bits (78), Expect = 0.007
Identities = 17/32 (53%), Positives = 19/32 (59%)
Frame = +3
Query: 57 MGKEKTHINIVVIGHVDSGKSTTTGHLIYKCG 152
MGK N+ VI HVD GKST T L+ K G
Sbjct: 13 MGKPSNVRNMSVIAHVDHGKSTLTDSLVQKAG 44
Score = 27.9 bits (59), Expect = 1.4
Identities = 11/32 (34%), Positives = 18/32 (56%)
Frame = +2
Query: 308 YYVTIIDAPGHRDFIKNMITGTSQADCAVLIV 403
+ V +ID+PGH DF + D A+++V
Sbjct: 98 FLVNLIDSPGHVDFSSEVTAALRVTDGALVVV 129
>SPAC513.01c |eft201|eft2-1, etf2, SPAPYUK71.04c|translation
elongation factor 2 |Schizosaccharomyces pombe|chr
1|||Manual
Length = 842
Score = 35.5 bits (78), Expect = 0.007
Identities = 17/32 (53%), Positives = 19/32 (59%)
Frame = +3
Query: 57 MGKEKTHINIVVIGHVDSGKSTTTGHLIYKCG 152
MGK N+ VI HVD GKST T L+ K G
Sbjct: 13 MGKPSNVRNMSVIAHVDHGKSTLTDSLVQKAG 44
Score = 27.9 bits (59), Expect = 1.4
Identities = 11/32 (34%), Positives = 18/32 (56%)
Frame = +2
Query: 308 YYVTIIDAPGHRDFIKNMITGTSQADCAVLIV 403
+ V +ID+PGH DF + D A+++V
Sbjct: 98 FLVNLIDSPGHVDFSSEVTAALRVTDGALVVV 129
>SPBC660.10 |||translation elongation factor G|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 813
Score = 34.7 bits (76), Expect = 0.013
Identities = 18/52 (34%), Positives = 24/52 (46%)
Frame = +2
Query: 263 GITIDIALWKFXTSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTG 418
GITI+ A F + +ID PGH DF + + D AV I+ G
Sbjct: 78 GITINSAAISFTWRNQRINLIDTPGHADFTFEVERSVAVLDGAVAIIDGSAG 129
Score = 28.7 bits (61), Expect = 0.83
Identities = 10/24 (41%), Positives = 17/24 (70%)
Frame = +3
Query: 81 NIVVIGHVDSGKSTTTGHLIYKCG 152
N+ +I H+D+GK+T T ++Y G
Sbjct: 30 NVGIIAHIDAGKTTLTEKMLYYGG 53
>SPBC1306.01c ||SPBC409.22c|translation elongation factor
G|Schizosaccharomyces pombe|chr 2|||Manual
Length = 770
Score = 34.3 bits (75), Expect = 0.017
Identities = 21/71 (29%), Positives = 32/71 (45%)
Frame = +2
Query: 308 YYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNGQTREHALLAFTLG 487
Y + IID PGH DF + D AVL++ A +G I+ + Q R + + +
Sbjct: 145 YNINIIDTPGHIDFTIEVERALRVLDGAVLVLCAVSGVQSQTITVDRQMRRYNVPRISFV 204
Query: 488 VKQLIVGXTKW 520
K +G W
Sbjct: 205 NKMDRMGADPW 215
Score = 29.1 bits (62), Expect = 0.63
Identities = 12/26 (46%), Positives = 17/26 (65%)
Frame = +3
Query: 81 NIVVIGHVDSGKSTTTGHLIYKCGGI 158
NI + H+DSGK+T T ++Y G I
Sbjct: 61 NIGISAHIDSGKTTFTERVLYYTGRI 86
>SPBC1271.15c |||translation initiation factor
IF-2Mt|Schizosaccharomyces pombe|chr 2|||Manual
Length = 686
Score = 32.7 bits (71), Expect = 0.051
Identities = 19/54 (35%), Positives = 25/54 (46%), Gaps = 2/54 (3%)
Frame = +2
Query: 263 GITIDIALWK--FXTSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTG 418
GIT I + F ++T +D PGH F G + AD VL+VA G
Sbjct: 203 GITQKIGAFTVPFDKGSKFITFLDTPGHMAFEAMRKRGANIADIVVLVVAGDDG 256
>SPBC17G9.09 |tif213||translation initiation factor eIF2 gamma
subunit|Schizosaccharomyces pombe|chr 2|||Manual
Length = 446
Score = 32.3 bits (70), Expect = 0.067
Identities = 17/65 (26%), Positives = 30/65 (46%)
Frame = +2
Query: 311 YVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNGQTREHALLAFTLGV 490
+V+ +D PGH + M+ G + D A+L++A QT EH + +
Sbjct: 109 HVSFVDCPGHDILMATMLNGAAVMDAALLLIAGNES------CPQPQTSEHLAAIEIMQL 162
Query: 491 KQLIV 505
K +I+
Sbjct: 163 KHIII 167
>SPAC1B3.04c |||mitochondrial GTPase Guf1 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 646
Score = 30.3 bits (65), Expect = 0.27
Identities = 12/45 (26%), Positives = 23/45 (51%)
Frame = +2
Query: 284 LWKFXTSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTG 418
++ + Y + +ID PGH DF ++ + + +L+V A G
Sbjct: 116 IYYYHGQSYLLNLIDTPGHVDFRAEVMHSLAACEGCILLVDASQG 160
>SPCC553.08c |||GTPase Ria1 |Schizosaccharomyces pombe|chr
3|||Manual
Length = 1000
Score = 30.3 bits (65), Expect = 0.27
Identities = 13/37 (35%), Positives = 21/37 (56%)
Frame = +2
Query: 308 YYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTG 418
Y + +ID+PGH DF + + + D A ++V A G
Sbjct: 94 YLINLIDSPGHVDFSSEVSSASRLCDGAFVLVDAVEG 130
>SPAC56F8.03 |||translation initiation factor IF2
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1079
Score = 29.5 bits (63), Expect = 0.47
Identities = 12/28 (42%), Positives = 16/28 (57%)
Frame = +2
Query: 320 IIDAPGHRDFIKNMITGTSQADCAVLIV 403
IID PGH F GTS + A+L++
Sbjct: 553 IIDTPGHESFTNLRSRGTSLCNIAILVI 580
>SPCC364.02c |bis1||stress response protein Bis1|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 384
Score = 28.3 bits (60), Expect = 1.1
Identities = 20/60 (33%), Positives = 28/60 (46%)
Frame = -1
Query: 331 SINDGNIVLASXELPESNIDCDTXLTSAFSLSNTQAYLKDPLPISWASFSNFSMVRLSIP 152
S++ + L S P S T LT A ++YL+ PL S S S + +R SIP
Sbjct: 305 SVDSASTALNSYSTPNSVSRKLTNLTPAARRLVARSYLRSPLHGSSPSASRHTALRTSIP 364
>SPBC215.12 |cwf10|spef2, snu114|GTPase Cwf10 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 983
Score = 26.6 bits (56), Expect = 3.3
Identities = 11/27 (40%), Positives = 16/27 (59%)
Frame = +2
Query: 323 IDAPGHRDFIKNMITGTSQADCAVLIV 403
ID PGH DF+ + + +D VL+V
Sbjct: 214 IDTPGHVDFVDEVAAPMAISDGVVLVV 240
>SPBC1703.06 |pof10||F-box protein Pof10|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 662
Score = 25.4 bits (53), Expect = 7.7
Identities = 15/43 (34%), Positives = 19/43 (44%)
Frame = -2
Query: 243 VCPIPKHI*RILYPFPGPPSRTSRWYVCQYHHICRSSDQWWWT 115
V +PK I I++ F P S S C+Y S D W T
Sbjct: 31 VLNLPKEILIIIFSFLDPRSLLSAQCTCKYWKKLLSDDLSWRT 73
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,627,939
Number of Sequences: 5004
Number of extensions: 49469
Number of successful extensions: 182
Number of sequences better than 10.0: 18
Number of HSP's better than 10.0 without gapping: 160
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 181
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 315915086
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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