Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= NV060497.seq
(685 letters)
Database: bee
438 sequences; 146,343 total letters
Searching......................................................done
Score E
Sequences producing significant alignments: (bits) Value
AF015267-1|AAC38959.1| 461|Apis mellifera elongation factor-1al... 174 7e-46
AY208278-1|AAO48970.1| 274|Apis mellifera elongation factor 1-a... 172 2e-45
X52884-1|CAA37066.1| 461|Apis mellifera elongation factor 1 alp... 170 9e-45
EF013389-1|ABK54743.1| 172|Apis mellifera elongation factor 1-a... 163 1e-42
AF069739-1|AAC63272.2| 690|Apis mellifera translation initiatio... 33 0.003
AM050259-1|CAJ18340.1| 683|Apis mellifera putative H3K9 methylt... 24 1.2
DQ244074-1|ABB36784.1| 517|Apis mellifera cytochrome P450 monoo... 23 2.7
AB253417-1|BAE86928.1| 567|Apis mellifera alpha-glucosidase pro... 23 2.7
D79208-1|BAA11466.1| 567|Apis mellifera alpha-glucosidase protein. 23 3.6
DQ869051-1|ABJ09598.1| 581|Apis mellifera pyrokinin-like recept... 22 6.3
>AF015267-1|AAC38959.1| 461|Apis mellifera elongation factor-1alpha
F2 protein.
Length = 461
Score = 174 bits (423), Expect = 7e-46
Identities = 88/119 (73%), Positives = 96/119 (80%)
Frame = +2
Query: 161 QTYHREVREGGPGNG*RILQICLGIGQTKG*GELGITIDIALWKFXTSKYYVTIIDAPGH 340
+ + +E +E G G+ + + + K E GITIDIALWKF TSKYYVTIIDAPGH
Sbjct: 40 EKFEKEAQEMGKGS----FKYAWVLDKLKAERERGITIDIALWKFETSKYYVTIIDAPGH 95
Query: 341 RDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNGQTREHALLAFTLGVKQLIVGXTK 517
RDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNGQTREHALLAFTLGVKQLIVG K
Sbjct: 96 RDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNGQTREHALLAFTLGVKQLIVGVNK 154
Score = 141 bits (342), Expect = 5e-36
Identities = 65/66 (98%), Positives = 65/66 (98%)
Frame = +3
Query: 57 MGKEKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVL 236
MGKEK HINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVL
Sbjct: 1 MGKEKIHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVL 60
Query: 237 DKLKAE 254
DKLKAE
Sbjct: 61 DKLKAE 66
Score = 39.9 bits (89), Expect = 2e-05
Identities = 25/58 (43%), Positives = 26/58 (44%)
Frame = +1
Query: 511 NKMDSTEPPYSEPRFXXXXXXXXXXXXXXXXXPAAVAFRAXFXDGTETTCLEPSXQMP 684
NKMDSTEPPYSE RF PAAVAF LE S +MP
Sbjct: 153 NKMDSTEPPYSETRFEEIKKEVSSYIKKIGYNPAAVAF-VPISGWHGDNMLEVSSKMP 209
>AY208278-1|AAO48970.1| 274|Apis mellifera elongation factor
1-alpha protein.
Length = 274
Score = 172 bits (419), Expect = 2e-45
Identities = 83/87 (95%), Positives = 83/87 (95%)
Frame = +2
Query: 257 ELGITIDIALWKFXTSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGI 436
E GITIDIALWKF TSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGI
Sbjct: 11 ERGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGI 70
Query: 437 SKNGQTREHALLAFTLGVKQLIVGXTK 517
SKNGQTREHALLAFTLGVKQLIVG K
Sbjct: 71 SKNGQTREHALLAFTLGVKQLIVGVNK 97
Score = 39.9 bits (89), Expect = 2e-05
Identities = 25/58 (43%), Positives = 26/58 (44%)
Frame = +1
Query: 511 NKMDSTEPPYSEPRFXXXXXXXXXXXXXXXXXPAAVAFRAXFXDGTETTCLEPSXQMP 684
NKMDSTEPPYSE RF PAAVAF LE S +MP
Sbjct: 96 NKMDSTEPPYSETRFEEIKKEVSSYIKKIGYNPAAVAF-VPISGWHGDNMLEVSSKMP 152
Score = 23.4 bits (48), Expect = 2.1
Identities = 9/9 (100%), Positives = 9/9 (100%)
Frame = +3
Query: 228 WVLDKLKAE 254
WVLDKLKAE
Sbjct: 1 WVLDKLKAE 9
>X52884-1|CAA37066.1| 461|Apis mellifera elongation factor 1 alpha
protein.
Length = 461
Score = 170 bits (414), Expect = 9e-45
Identities = 86/119 (72%), Positives = 95/119 (79%)
Frame = +2
Query: 161 QTYHREVREGGPGNG*RILQICLGIGQTKG*GELGITIDIALWKFXTSKYYVTIIDAPGH 340
+ + +E +E G G+ + + + K E GITIDIALWKF T+KYYVTIIDAPGH
Sbjct: 40 EKFEKEAQEMGKGS----FKYAWVLDKLKAERERGITIDIALWKFETAKYYVTIIDAPGH 95
Query: 341 RDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNGQTREHALLAFTLGVKQLIVGXTK 517
RDFIKNMITGTSQADCAVLIVAAG GEFEAGISKNGQTREHALLAFTLGVKQLIVG K
Sbjct: 96 RDFIKNMITGTSQADCAVLIVAAGIGEFEAGISKNGQTREHALLAFTLGVKQLIVGVNK 154
Score = 141 bits (342), Expect = 5e-36
Identities = 65/66 (98%), Positives = 65/66 (98%)
Frame = +3
Query: 57 MGKEKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVL 236
MGKEK HINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVL
Sbjct: 1 MGKEKIHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVL 60
Query: 237 DKLKAE 254
DKLKAE
Sbjct: 61 DKLKAE 66
Score = 33.5 bits (73), Expect = 0.002
Identities = 21/58 (36%), Positives = 24/58 (41%)
Frame = +1
Query: 511 NKMDSTEPPYSEPRFXXXXXXXXXXXXXXXXXPAAVAFRAXFXDGTETTCLEPSXQMP 684
NKMD T+PPYSE RF A+VAF LEPS + P
Sbjct: 153 NKMDMTDPPYSEARFEEIKKEVSSYIKKIGYNTASVAF-VPISGWHGDNMLEPSPKTP 209
>EF013389-1|ABK54743.1| 172|Apis mellifera elongation factor
1-alpha protein.
Length = 172
Score = 163 bits (397), Expect = 1e-42
Identities = 78/81 (96%), Positives = 78/81 (96%)
Frame = +2
Query: 275 DIALWKFXTSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNGQT 454
DIALWKF TSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNGQT
Sbjct: 1 DIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNGQT 60
Query: 455 REHALLAFTLGVKQLIVGXTK 517
REHALLAFTLGVKQLIVG K
Sbjct: 61 REHALLAFTLGVKQLIVGVNK 81
Score = 39.9 bits (89), Expect = 2e-05
Identities = 25/58 (43%), Positives = 26/58 (44%)
Frame = +1
Query: 511 NKMDSTEPPYSEPRFXXXXXXXXXXXXXXXXXPAAVAFRAXFXDGTETTCLEPSXQMP 684
NKMDSTEPPYSE RF PAAVAF LE S +MP
Sbjct: 80 NKMDSTEPPYSETRFEEIKKEVSSYIKKIGYNPAAVAF-VPISGWHGDNMLEVSSKMP 136
>AF069739-1|AAC63272.2| 690|Apis mellifera translation initiation
factor 2 protein.
Length = 690
Score = 33.1 bits (72), Expect = 0.003
Identities = 16/35 (45%), Positives = 18/35 (51%)
Frame = +2
Query: 314 VTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTG 418
VT +D PGH FI G D VL+VAA G
Sbjct: 195 VTFLDTPGHAAFISMRHRGAHITDIVVLVVAADDG 229
Score = 25.4 bits (53), Expect = 0.51
Identities = 9/18 (50%), Positives = 13/18 (72%)
Frame = +3
Query: 69 KTHINIVVIGHVDSGKST 122
K H + ++GHVD GK+T
Sbjct: 143 KRHPIVTIMGHVDHGKTT 160
>AM050259-1|CAJ18340.1| 683|Apis mellifera putative H3K9
methyltransferase protein.
Length = 683
Score = 24.2 bits (50), Expect = 1.2
Identities = 11/15 (73%), Positives = 11/15 (73%)
Frame = +3
Query: 78 INIVVIGHVDSGKST 122
INI IGHV GKST
Sbjct: 43 INIGTIGHVAHGKST 57
>DQ244074-1|ABB36784.1| 517|Apis mellifera cytochrome P450
monooxygenase protein.
Length = 517
Score = 23.0 bits (47), Expect = 2.7
Identities = 10/17 (58%), Positives = 12/17 (70%)
Frame = -1
Query: 70 FSLPIFG*SRITNCV*Y 20
FSLPIFG I +C+ Y
Sbjct: 57 FSLPIFGTRWIFSCIGY 73
>AB253417-1|BAE86928.1| 567|Apis mellifera alpha-glucosidase
protein.
Length = 567
Score = 23.0 bits (47), Expect = 2.7
Identities = 13/34 (38%), Positives = 18/34 (52%)
Frame = +2
Query: 299 TSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLI 400
T KYY D P + FIKN+ ++ +D LI
Sbjct: 294 TMKYYDYGADFPFNFAFIKNVSRDSNSSDFKKLI 327
>D79208-1|BAA11466.1| 567|Apis mellifera alpha-glucosidase protein.
Length = 567
Score = 22.6 bits (46), Expect = 3.6
Identities = 12/34 (35%), Positives = 18/34 (52%)
Frame = +2
Query: 299 TSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLI 400
T KYY D P + FIKN+ ++ +D L+
Sbjct: 294 TMKYYDYGADFPFNFAFIKNVSRDSNSSDFKKLV 327
>DQ869051-1|ABJ09598.1| 581|Apis mellifera pyrokinin-like receptor
2 protein.
Length = 581
Score = 21.8 bits (44), Expect = 6.3
Identities = 13/46 (28%), Positives = 17/46 (36%)
Frame = -1
Query: 574 LPS*FXQIWAHCMVVQWNPFCXSYDELFDTEGESEQGMLTGLTVLR 437
LP IW+H V FC +T + +T TV R
Sbjct: 107 LPPEIYYIWSHFPYVFGEAFCIIQSFAAETSANATVLTITAFTVER 152
Database: bee
Posted date: Oct 23, 2007 1:17 PM
Number of letters in database: 146,343
Number of sequences in database: 438
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 180,731
Number of Sequences: 438
Number of extensions: 3299
Number of successful extensions: 27
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 15
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 23
length of database: 146,343
effective HSP length: 56
effective length of database: 121,815
effective search space used: 20830365
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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