Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= NV060493.seq
(553 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC25H2.07 |tif11||translation initiation factor eIF1A|Schizosa... 109 2e-25
SPAC18G6.02c |chp1||chromodomain protein Chp1|Schizosaccharomyce... 28 0.80
SPCC663.15c |||conserved fungal protein|Schizosaccharomyces pomb... 28 0.80
SPAPB15E9.02c |||dubious|Schizosaccharomyces pombe|chr 1|||Manual 28 1.1
SPBC27B12.04c |||conserved eukaryotic protein|Schizosaccharomyce... 27 1.8
SPBC4C3.06 |||actin cytoskeletal protein Syp1|Schizosaccharomyce... 26 4.2
SPCC1620.14c |snf22|SPCC830.01c|ATP-dependent DNA helicase Snf22... 25 7.4
SPAC18G6.10 |||chromosome segregation protein |Schizosaccharomyc... 25 7.4
SPCC330.07c |||membrane transporter|Schizosaccharomyces pombe|ch... 25 9.8
>SPBC25H2.07 |tif11||translation initiation factor
eIF1A|Schizosaccharomyces pombe|chr 2|||Manual
Length = 138
Score = 109 bits (263), Expect = 2e-25
Identities = 51/74 (68%), Positives = 58/74 (78%), Gaps = 1/74 (1%)
Frame = +1
Query: 259 HIRGKLRKKVWINQGDIILIGLRDYQDAKADVILKYTPDEARNLKTYGEFPETVRINETV 438
HIRGKLRKKVWINQGDIIL+ LR++QD K DVILKYT DEAR LK GE PET +INET
Sbjct: 60 HIRGKLRKKVWINQGDIILLSLREFQDEKGDVILKYTADEARTLKNQGELPETAKINETD 119
Query: 439 VYSVDGLDE-DIEF 477
+ +G D+ D EF
Sbjct: 120 TFGAEGEDDLDFEF 133
Score = 60.5 bits (140), Expect = 2e-10
Identities = 27/43 (62%), Positives = 31/43 (72%)
Frame = +2
Query: 158 LVFKEDGQEYAQVTKMLGNGRLEAMCFDGIKRRVTSEGNYEKK 286
L + E+GQ YAQVTKMLGNGR+EA CFDG+KR G KK
Sbjct: 26 LTYAEEGQMYAQVTKMLGNGRIEAACFDGVKRLGHIRGKLRKK 68
>SPAC18G6.02c |chp1||chromodomain protein Chp1|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 960
Score = 28.3 bits (60), Expect = 0.80
Identities = 22/73 (30%), Positives = 37/73 (50%), Gaps = 4/73 (5%)
Frame = +1
Query: 328 DYQDAKADV-ILKYTPDEARNLKTYG---EFPETVRINETVVYSVDGLDEDIEFGDEVSS 495
D ++ K ILK TP + N ++ +T+ +E +VD LDE+ +F V++
Sbjct: 388 DMEECKVSKSILKTTPSKKANARSVSFTQTTTDTLSESEKFASNVD-LDENFDFNVNVTN 446
Query: 496 EDEAVMCEYVIXS 534
ED + + VI S
Sbjct: 447 EDAKQLKKSVIGS 459
>SPCC663.15c |||conserved fungal protein|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 657
Score = 28.3 bits (60), Expect = 0.80
Identities = 16/60 (26%), Positives = 26/60 (43%)
Frame = -1
Query: 250 FDAIKAHGLQAAITEHLCDLGVLLSVFLKDQLTFFSFIFVLSSPPIFSSFTFVFRHVLYD 71
FDA H + + LCD L V + F++ I+ FS + F++ L+D
Sbjct: 418 FDAALTHRDRECLIHDLCDNDQLNDVVREFMNAFYNIIYEAHQAADFSQAIYDFQYFLWD 477
>SPAPB15E9.02c |||dubious|Schizosaccharomyces pombe|chr 1|||Manual
Length = 188
Score = 27.9 bits (59), Expect = 1.1
Identities = 14/52 (26%), Positives = 29/52 (55%), Gaps = 3/52 (5%)
Frame = -1
Query: 193 LGVLLSVFLKDQLTFFSFIFVLSSPPIFSSFTFVFRHVLYDFYY---KHKQL 47
+ + + + + + FF F F S +FS F+F+F + ++F++ +HK L
Sbjct: 93 IAIFIHPYDSNVVPFFCFFFYFS---LFSFFSFLFTSLHFNFFFRLCRHKHL 141
>SPBC27B12.04c |||conserved eukaryotic protein|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 817
Score = 27.1 bits (57), Expect = 1.8
Identities = 13/42 (30%), Positives = 23/42 (54%)
Frame = +1
Query: 334 QDAKADVILKYTPDEARNLKTYGEFPETVRINETVVYSVDGL 459
++ K++V ++YT D A GE ++ E V+YS+ L
Sbjct: 511 RNRKSNVKIEYTEDFATGFAISGEISHSINNLEFVLYSLSSL 552
>SPBC4C3.06 |||actin cytoskeletal protein Syp1|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 818
Score = 25.8 bits (54), Expect = 4.2
Identities = 26/91 (28%), Positives = 41/91 (45%), Gaps = 12/91 (13%)
Frame = +3
Query: 96 KVKEEKIGGEERTKMKLKN-VSWSLRKTDKSTPKSQRCSVMAAWRPCALMA--------- 245
K KEEK GE + KLKN +S R+ K ++ S + RP L A
Sbjct: 259 KSKEEKSHGENESGGKLKNKMSTLFRRKTIMPKKDKKPSHKSNGRPNKLTAFFNKNSKAS 318
Query: 246 --SNAVSHPRETTKKSVDKSRRHNINRPTRL 332
S+A HP S+++ R ++ N +++
Sbjct: 319 SISSAEEHPSNIDDSSIER-RHYDSNHSSQI 348
>SPCC1620.14c |snf22|SPCC830.01c|ATP-dependent DNA helicase
Snf22|Schizosaccharomyces pombe|chr 3|||Manual
Length = 1680
Score = 25.0 bits (52), Expect = 7.4
Identities = 9/25 (36%), Positives = 15/25 (60%)
Frame = +3
Query: 144 LKNVSWSLRKTDKSTPKSQRCSVMA 218
L++ +W + D ST RCS++A
Sbjct: 1223 LRSKNWKYLRLDGSTKSDDRCSLLA 1247
>SPAC18G6.10 |||chromosome segregation protein |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 688
Score = 25.0 bits (52), Expect = 7.4
Identities = 12/29 (41%), Positives = 18/29 (62%)
Frame = -3
Query: 428 LMRTVSGNSPYVFRFLASSGVYFKMTSAF 342
L++ V N ++F LA SGV FK+ + F
Sbjct: 539 LIKFVWRNKVFLFGILALSGVIFKLINFF 567
>SPCC330.07c |||membrane transporter|Schizosaccharomyces pombe|chr
3|||Manual
Length = 500
Score = 24.6 bits (51), Expect = 9.8
Identities = 9/21 (42%), Positives = 13/21 (61%)
Frame = -1
Query: 121 PPIFSSFTFVFRHVLYDFYYK 59
P + SSF +F H+LY Y+
Sbjct: 160 PMVVSSFAHIFGHLLYCMAYR 180
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 1,978,840
Number of Sequences: 5004
Number of extensions: 35597
Number of successful extensions: 102
Number of sequences better than 10.0: 9
Number of HSP's better than 10.0 without gapping: 97
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 102
length of database: 2,362,478
effective HSP length: 69
effective length of database: 2,017,202
effective search space used: 229961028
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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