Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= NV060493.seq
(553 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g35680.2 68418.m04264 eukaryotic translation initiation facto... 112 2e-25
At5g35680.1 68418.m04263 eukaryotic translation initiation facto... 112 2e-25
At2g04520.1 68415.m00458 eukaryotic translation initiation facto... 109 1e-24
At3g48710.1 68416.m05319 expressed protein putative protein - Ar... 30 1.2
At4g38270.1 68417.m05406 glycosyl transferase family 8 protein c... 28 4.8
At2g03250.1 68415.m00278 EXS family protein / ERD1/XPR1/SYG1 fam... 28 4.8
At5g40360.1 68418.m04896 myb family transcription factor (MYB115... 27 6.3
At5g35180.1 68418.m04169 expressed protein 27 8.3
At2g07690.1 68415.m00993 minichromosome maintenance family prote... 27 8.3
>At5g35680.2 68418.m04264 eukaryotic translation initiation factor
1A, putative / eIF-1A, putative / eIF-4C, putative
strong similarity to translation initiation factor
(eIF-1A) [Beta vulgaris] GI:17977975; contains Pfam
profile PF01176: Eukaryotic initiation factor 1A
Length = 145
Score = 112 bits (269), Expect = 2e-25
Identities = 53/85 (62%), Positives = 62/85 (72%)
Frame = +1
Query: 256 CHIRGKLRKKVWINQGDIILIGLRDYQDAKADVILKYTPDEARNLKTYGEFPETVRINET 435
CHIRGK+ KKVWI GDIIL+GLRDYQD KADVILKY DEAR LK YGE PE R+NE
Sbjct: 59 CHIRGKMHKKVWIAAGDIILVGLRDYQDDKADVILKYMSDEARLLKAYGELPENTRLNEG 118
Query: 436 VVYSVDGLDEDIEFGDEVSSEDEAV 510
+V +D D+D+ D + EDE +
Sbjct: 119 IVGDLDD-DDDVVGEDYIEFEDEDI 142
Score = 62.9 bits (146), Expect = 1e-10
Identities = 27/43 (62%), Positives = 31/43 (72%)
Frame = +2
Query: 158 LVFKEDGQEYAQVTKMLGNGRLEAMCFDGIKRRVTSEGNYEKK 286
L+FKEDGQEYAQV +MLGNGR + MC DG+KR G KK
Sbjct: 26 LIFKEDGQEYAQVLRMLGNGRCDVMCIDGVKRLCHIRGKMHKK 68
>At5g35680.1 68418.m04263 eukaryotic translation initiation factor
1A, putative / eIF-1A, putative / eIF-4C, putative
strong similarity to translation initiation factor
(eIF-1A) [Beta vulgaris] GI:17977975; contains Pfam
profile PF01176: Eukaryotic initiation factor 1A
Length = 145
Score = 112 bits (269), Expect = 2e-25
Identities = 53/85 (62%), Positives = 62/85 (72%)
Frame = +1
Query: 256 CHIRGKLRKKVWINQGDIILIGLRDYQDAKADVILKYTPDEARNLKTYGEFPETVRINET 435
CHIRGK+ KKVWI GDIIL+GLRDYQD KADVILKY DEAR LK YGE PE R+NE
Sbjct: 59 CHIRGKMHKKVWIAAGDIILVGLRDYQDDKADVILKYMSDEARLLKAYGELPENTRLNEG 118
Query: 436 VVYSVDGLDEDIEFGDEVSSEDEAV 510
+V +D D+D+ D + EDE +
Sbjct: 119 IVGDLDD-DDDVVGEDYIEFEDEDI 142
Score = 62.9 bits (146), Expect = 1e-10
Identities = 27/43 (62%), Positives = 31/43 (72%)
Frame = +2
Query: 158 LVFKEDGQEYAQVTKMLGNGRLEAMCFDGIKRRVTSEGNYEKK 286
L+FKEDGQEYAQV +MLGNGR + MC DG+KR G KK
Sbjct: 26 LIFKEDGQEYAQVLRMLGNGRCDVMCIDGVKRLCHIRGKMHKK 68
>At2g04520.1 68415.m00458 eukaryotic translation initiation factor
1A, putative / eIF-1A, putative / eIF-4C, putative
strong similarity to translation initiation factor
(eIF-1A) [Beta vulgaris] GI:17977975; contains Pfam
profile PF01176: Eukaryotic initiation factor 1A
Length = 145
Score = 109 bits (262), Expect = 1e-24
Identities = 52/85 (61%), Positives = 61/85 (71%)
Frame = +1
Query: 256 CHIRGKLRKKVWINQGDIILIGLRDYQDAKADVILKYTPDEARNLKTYGEFPETVRINET 435
CHIRGK+ KKVWI GDI+L+GLRDYQD KADVILKY DEAR LK YGE PE R+NE
Sbjct: 59 CHIRGKMHKKVWIAAGDIVLVGLRDYQDDKADVILKYMSDEARLLKAYGELPENTRLNEG 118
Query: 436 VVYSVDGLDEDIEFGDEVSSEDEAV 510
+V ++ D+D D V EDE +
Sbjct: 119 IVGDLED-DDDNNDDDYVEFEDEDI 142
Score = 64.1 bits (149), Expect = 6e-11
Identities = 29/43 (67%), Positives = 31/43 (72%)
Frame = +2
Query: 158 LVFKEDGQEYAQVTKMLGNGRLEAMCFDGIKRRVTSEGNYEKK 286
L+FKEDGQEYAQV +MLGNGR EAMC DG KR G KK
Sbjct: 26 LIFKEDGQEYAQVLRMLGNGRCEAMCIDGTKRLCHIRGKMHKK 68
>At3g48710.1 68416.m05319 expressed protein putative protein -
Arabidopsis thaliana, EMBL:AL078465.1
Length = 462
Score = 29.9 bits (64), Expect = 1.2
Identities = 22/80 (27%), Positives = 32/80 (40%), Gaps = 2/80 (2%)
Frame = +3
Query: 96 KVKEEKIGGEERTKMK--LKNVSWSLRKTDKSTPKSQRCSVMAAWRPCALMASNAVSHPR 269
+ K+EK EE +K K+ S R+ DKST S + + S P+
Sbjct: 308 RTKKEKPAAEEEKSIKGSAKSSRKSFRQVDKSTTSSSKKQKVDKDDSSKEKGKTQTSKPQ 367
Query: 270 ETTKKSVDKSRRHNINRPTR 329
K +SR+ PTR
Sbjct: 368 AKGSKDQGQSRKKGKKEPTR 387
>At4g38270.1 68417.m05406 glycosyl transferase family 8 protein
contains Pfam profile: PF01501 glycosyl transferase
family 8
Length = 680
Score = 27.9 bits (59), Expect = 4.8
Identities = 13/48 (27%), Positives = 21/48 (43%)
Frame = +1
Query: 325 RDYQDAKADVILKYTPDEARNLKTYGEFPETVRINETVVYSVDGLDED 468
RDY+ AD ILK D+ K Y ++ + V+ + E+
Sbjct: 207 RDYESPNADAILKLMRDQIIMAKAYANIAKSKNVTNLYVFLMQQCGEN 254
>At2g03250.1 68415.m00278 EXS family protein / ERD1/XPR1/SYG1 family
protein similar to PHO1 protein [Arabidopsis thaliana]
GI:20069032; contains Pfam profiles PF03105: SPX domain,
PF03124: EXS family
Length = 756
Score = 27.9 bits (59), Expect = 4.8
Identities = 12/30 (40%), Positives = 18/30 (60%)
Frame = -1
Query: 166 KDQLTFFSFIFVLSSPPIFSSFTFVFRHVL 77
KD F +F+F++S+ P FS F R +L
Sbjct: 562 KDSQVFNTFLFIVSAFPFFSRFLQCMRRML 591
>At5g40360.1 68418.m04896 myb family transcription factor (MYB115)
contains Pfam profile: PF00249 myb-like DNA-binding
domain
Length = 359
Score = 27.5 bits (58), Expect = 6.3
Identities = 15/50 (30%), Positives = 22/50 (44%)
Frame = +1
Query: 241 WHQTPCHIRGKLRKKVWINQGDIILIGLRDYQDAKADVILKYTPDEARNL 390
WH H+R ++K W + D ILI + K I K P + N+
Sbjct: 200 WHN---HLRPNIKKNDWSEEEDQILIEVHKIVGNKWTEIAKRLPGRSENI 246
>At5g35180.1 68418.m04169 expressed protein
Length = 778
Score = 27.1 bits (57), Expect = 8.3
Identities = 11/25 (44%), Positives = 15/25 (60%)
Frame = +3
Query: 135 KMKLKNVSWSLRKTDKSTPKSQRCS 209
K+KLKNVSW++ PK+ S
Sbjct: 506 KVKLKNVSWAIASLSLKRPKAPGAS 530
>At2g07690.1 68415.m00993 minichromosome maintenance family protein
/ MCM family protein similar to SP|P55862 DNA
replication licensing factor MCM5 (CDC46 homolog)
{Xenopus laevis}; contains Pfam profile PF00493:
MCM2/3/5 family
Length = 727
Score = 27.1 bits (57), Expect = 8.3
Identities = 18/64 (28%), Positives = 31/64 (48%), Gaps = 1/64 (1%)
Frame = +1
Query: 286 VWINQGDIILIGLRDYQDAKADVILKYTPDEARNLKTYGEFPETVR-INETVVYSVDGLD 462
V I Q I ++GL D +A + +TPDE K + + + + I + S+ G
Sbjct: 282 VAIRQPYIRVVGLEDTNEASSRGPANFTPDEEEEFKKFADSQDVYKNICTKIAPSIFG-H 340
Query: 463 EDIE 474
ED++
Sbjct: 341 EDVK 344
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 10,274,862
Number of Sequences: 28952
Number of extensions: 186551
Number of successful extensions: 673
Number of sequences better than 10.0: 9
Number of HSP's better than 10.0 without gapping: 661
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 673
length of database: 12,070,560
effective HSP length: 77
effective length of database: 9,841,256
effective search space used: 1043173136
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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