BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= NV060488.seq (686 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBC1539.07c |||glutathione-dependent formaldehyde dehydrogenase... 113 3e-26 SPCC13B11.04c ||SPCC777.01c|glutathione-dependent formaldehyde d... 109 5e-25 SPCC13B11.01 |adh1|adh|alcohol dehydrogenase Adh1|Schizosaccharo... 33 0.029 SPBC1773.05c |tms1||hexitol dehydrogenase |Schizosaccharomyces p... 28 1.1 SPBC2G2.09c |crs1|mug17|meiosis specific cyclin Crs1|Schizosacch... 26 4.4 SPAC20H4.10 |ufd2||ubiquitin-protein ligase E4 |Schizosaccharomy... 26 4.4 SPAC20H4.01 ||SPAC631.03|U3 snoRNP-associated protein Utp5|Schiz... 25 7.8 >SPBC1539.07c |||glutathione-dependent formaldehyde dehydrogenase |Schizosaccharomyces pombe|chr 2|||Manual Length = 378 Score = 113 bits (271), Expect = 3e-26 Identities = 48/88 (54%), Positives = 59/88 (67%) Frame = +1 Query: 256 DAYTLSGKDPEGVFPVVLXXXXXXXXXXXXXXXTSVKPGDHVVPLYVPQCNTCKFCLNPK 435 DAYTLSG DPEG FP+VL +V+PGDHV+ LY P+C CKFC + K Sbjct: 50 DAYTLSGVDPEGAFPIVLGHEGAGIVESIGEGVINVRPGDHVILLYTPECKECKFCRSGK 109 Query: 436 TNLCQKVRSTQGQGVMPDGTRRFRLKDR 519 TNLC K+R TQG+G+MPDGT RF +D+ Sbjct: 110 TNLCSKIRETQGRGLMPDGTSRFSCRDK 137 Score = 65.7 bits (153), Expect = 6e-12 Identities = 31/48 (64%), Positives = 36/48 (75%) Frame = +2 Query: 110 MSTVGKVIKCLAAVAWEAGKPLSIEEIEVDPPKAGEVRVKITATGVCH 253 MS GK I C AAVAW A +PLSIE+I+V PPKA EVRVK+ + VCH Sbjct: 1 MSFEGKTITCKAAVAWGAKEPLSIEDIQVAPPKAHEVRVKVDWSAVCH 48 Score = 47.2 bits (107), Expect = 2e-06 Identities = 19/37 (51%), Positives = 28/37 (75%) Frame = +3 Query: 510 KGQELYHFMGCSTFSQYTVVLEISLCKVAEAAPLDKV 620 + + L H+MGCS+FSQYTVV +ISL ++ +APL + Sbjct: 135 RDKTLLHYMGCSSFSQYTVVADISLVAISHSAPLRSI 171 Score = 27.9 bits (59), Expect = 1.5 Identities = 11/15 (73%), Positives = 12/15 (80%) Frame = +2 Query: 617 SFVLLGCGVPTGYGA 661 S LLGCGV TG+GA Sbjct: 170 SICLLGCGVTTGFGA 184 >SPCC13B11.04c ||SPCC777.01c|glutathione-dependent formaldehyde dehydrogenase|Schizosaccharomyces pombe|chr 3|||Manual Length = 380 Score = 109 bits (261), Expect = 5e-25 Identities = 47/83 (56%), Positives = 59/83 (71%) Frame = +1 Query: 256 DAYTLSGKDPEGVFPVVLXXXXXXXXXXXXXXXTSVKPGDHVVPLYVPQCNTCKFCLNPK 435 DAYTLSGKDPEG+FPV+L T+V+ GD V+ LY P+C TCKFC + K Sbjct: 53 DAYTLSGKDPEGLFPVILGHEGAGIVESVGPQVTTVQVGDPVIALYTPECKTCKFCKSGK 112 Query: 436 TNLCQKVRSTQGQGVMPDGTRRF 504 TNLC ++R+TQG+G+MPDGT RF Sbjct: 113 TNLCGRIRTTQGKGLMPDGTSRF 135 Score = 65.7 bits (153), Expect = 6e-12 Identities = 27/46 (58%), Positives = 34/46 (73%) Frame = +2 Query: 116 TVGKVIKCLAAVAWEAGKPLSIEEIEVDPPKAGEVRVKITATGVCH 253 T GK+I C AAVAW+ PLSIE ++V PP+ EVR+KI +GVCH Sbjct: 6 TAGKIINCKAAVAWQPAAPLSIENVQVFPPRVHEVRIKIVNSGVCH 51 Score = 51.2 bits (117), Expect = 1e-07 Identities = 22/36 (61%), Positives = 26/36 (72%) Frame = +3 Query: 513 GQELYHFMGCSTFSQYTVVLEISLCKVAEAAPLDKV 620 G L HFMGCSTFS+YTVV +IS+ + APLD V Sbjct: 139 GNTLLHFMGCSTFSEYTVVADISVVAIERLAPLDSV 174 Score = 30.3 bits (65), Expect = 0.27 Identities = 12/16 (75%), Positives = 13/16 (81%) Frame = +2 Query: 617 SFVLLGCGVPTGYGAA 664 S LLGCG+ TGYGAA Sbjct: 173 SVCLLGCGITTGYGAA 188 >SPCC13B11.01 |adh1|adh|alcohol dehydrogenase Adh1|Schizosaccharomyces pombe|chr 3|||Manual Length = 350 Score = 33.5 bits (73), Expect = 0.029 Identities = 22/48 (45%), Positives = 27/48 (56%), Gaps = 2/48 (4%) Frame = +2 Query: 134 KCLAAVAWEAGKPLSI--EEIEVDPPKAGEVRVKITATGVCHMTRIHS 271 K LAAV G P ++ EE+ V P EV V I TGVCH T +H+ Sbjct: 6 KQLAAVFHTHGGPENVKFEEVPVAEPGQDEVLVNIKYTGVCH-TDLHA 52 >SPBC1773.05c |tms1||hexitol dehydrogenase |Schizosaccharomyces pombe|chr 2|||Manual Length = 360 Score = 28.3 bits (60), Expect = 1.1 Identities = 12/31 (38%), Positives = 17/31 (54%) Frame = +1 Query: 355 TSVKPGDHVVPLYVPQCNTCKFCLNPKTNLC 447 +S+KPGD V C C +C + + NLC Sbjct: 81 SSLKPGDPVAVEPGCVCRLCDYCRSGRYNLC 111 >SPBC2G2.09c |crs1|mug17|meiosis specific cyclin Crs1|Schizosaccharomyces pombe|chr 2|||Manual Length = 229 Score = 26.2 bits (55), Expect = 4.4 Identities = 15/38 (39%), Positives = 20/38 (52%), Gaps = 2/38 (5%) Frame = +3 Query: 12 LIGF*YFVTS--SSKRFIARL*FQHFVIGSCPVQSCQQ 119 L+ F +V S S + F+ L FQH S P +SC Q Sbjct: 177 LLNFDIYVISLPSVESFLTPLIFQHVFFKSLPSESCDQ 214 >SPAC20H4.10 |ufd2||ubiquitin-protein ligase E4 |Schizosaccharomyces pombe|chr 1|||Manual Length = 1010 Score = 26.2 bits (55), Expect = 4.4 Identities = 9/24 (37%), Positives = 15/24 (62%) Frame = -3 Query: 312 SQYYRKHSLRIFSGECIRVIWQTP 241 +Q+Y K ++R + E R IW+ P Sbjct: 686 TQFYDKFNIRFYICEVFRTIWKQP 709 >SPAC20H4.01 ||SPAC631.03|U3 snoRNP-associated protein Utp5|Schizosaccharomyces pombe|chr 1|||Manual Length = 666 Score = 25.4 bits (53), Expect = 7.8 Identities = 15/38 (39%), Positives = 23/38 (60%), Gaps = 1/38 (2%) Frame = +2 Query: 134 KCLAAVAWEAGKPL-SIEEIEVDPPKAGEVRVKITATG 244 K ++ +AWE KPL + E+I D +GE+ V T +G Sbjct: 63 KVISCIAWEQ-KPLYASEQITTDISGSGEILVLGTNSG 99 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,805,708 Number of Sequences: 5004 Number of extensions: 55785 Number of successful extensions: 144 Number of sequences better than 10.0: 7 Number of HSP's better than 10.0 without gapping: 133 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 143 length of database: 2,362,478 effective HSP length: 70 effective length of database: 2,012,198 effective search space used: 317927284 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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