Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= NV060486.seq
(684 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At2g44350.2 68415.m05517 citrate synthase, mitochondrial, putati... 99 1e-21
At2g44350.1 68415.m05516 citrate synthase, mitochondrial, putati... 99 1e-21
At3g60100.1 68416.m06711 citrate synthase, mitochondrial, putati... 100 2e-21
At3g58740.1 68416.m06547 citrate synthase, glyoxysomal, putative... 35 0.058
At5g36250.1 68418.m04373 protein phosphatase 2C, putative / PP2C... 32 0.41
At4g15096.1 68417.m02320 hypothetical protein 29 2.2
At5g53030.2 68418.m06586 expressed protein 29 2.9
At5g53030.1 68418.m06587 expressed protein 29 2.9
At2g40955.1 68415.m05057 hypothetical protein 29 2.9
At2g11270.1 68415.m01208 citrate synthase-related contains simil... 29 3.8
At4g16442.1 68417.m02489 integral membrane family protein contai... 28 5.0
At4g11560.1 68417.m01853 bromo-adjacent homology (BAH) domain-co... 28 6.6
At3g15750.1 68416.m01995 expressed protein 28 6.6
At1g59650.1 68414.m06709 expressed protein ; supporting cDNA gi|... 27 8.8
>At2g44350.2 68415.m05517 citrate synthase, mitochondrial, putative
strong similarity to SP|P20115 Citrate synthase,
mitochondrial precursor {Arabidopsis thaliana};
contains Pfam profile PF00285: Citrate synthase
Length = 474
Score = 99 bits (238), Expect = 1e-21
Identities = 55/141 (39%), Positives = 73/141 (51%), Gaps = 1/141 (0%)
Frame = +2
Query: 260 VTVDMMYGGMRGIKGLVWETSVLDADEGIRFRGLSIPECQQQLPKAKGGEEPLPEVFSGF 439
+TVDM+ GGMRG+ GL+WETS+LD +EGIRFRGLSIPECQ+ LP A+ G EPLPE
Sbjct: 71 ITVDMVIGGMRGMTGLLWETSLLDPEEGIRFRGLSIPECQKVLPTAQSGAEPLPEGLLWL 130
Query: 440 SSLAIYPPKRK*SAV*R-MGGEGGYRLT*XQC*TXCPANCIPCRSSRLPSPHSTVNLNXL 616
P K + A+ + + P+ P V
Sbjct: 131 LLTGKVPSKEQVEALSKDLANRAAVPDYVYNAIDALPSTAHPMTQFASGVMALQVQSEFQ 190
Query: 617 KPTQXGVHKSQYWEYVYXDTM 679
K + G+HKS++WE Y D +
Sbjct: 191 KAYENGIHKSKFWEPTYEDCL 211
Score = 60.1 bits (139), Expect = 1e-09
Identities = 27/70 (38%), Positives = 43/70 (61%), Gaps = 1/70 (1%)
Frame = +1
Query: 427 LFWLLVTGDIPTEAQVKRCLKN-GRRGRIPAHVVXMLNXMPGKLHPMSQFSAAVTALNSE 603
L WLL+TG +P++ QV+ K+ R +P +V ++ +P HPM+QF++ V AL +
Sbjct: 127 LLWLLLTGKVPSKEQVEALSKDLANRAAVPDYVYNAIDALPSTAHPMTQFASGVMALQVQ 186
Query: 604 SKXAKAYSXG 633
S+ KAY G
Sbjct: 187 SEFQKAYENG 196
Score = 34.7 bits (76), Expect = 0.058
Identities = 16/62 (25%), Positives = 36/62 (58%)
Frame = +3
Query: 69 MALFRITSSRLVELQKACPTATVLLRGLSAEQTNLKSILQEKIPKEQEKIREFRKKHGST 248
++ F SR+ Q + + ++ S+ +LKS LQE IP++Q+++++ + +HG
Sbjct: 7 VSAFTRLRSRVQGQQSSLSNSVRWIQMQSSTDLDLKSQLQELIPEQQDRLKKLKSEHGKV 66
Query: 249 KV 254
++
Sbjct: 67 QL 68
>At2g44350.1 68415.m05516 citrate synthase, mitochondrial, putative
strong similarity to SP|P20115 Citrate synthase,
mitochondrial precursor {Arabidopsis thaliana};
contains Pfam profile PF00285: Citrate synthase
Length = 473
Score = 99 bits (238), Expect = 1e-21
Identities = 55/141 (39%), Positives = 73/141 (51%), Gaps = 1/141 (0%)
Frame = +2
Query: 260 VTVDMMYGGMRGIKGLVWETSVLDADEGIRFRGLSIPECQQQLPKAKGGEEPLPEVFSGF 439
+TVDM+ GGMRG+ GL+WETS+LD +EGIRFRGLSIPECQ+ LP A+ G EPLPE
Sbjct: 70 ITVDMVIGGMRGMTGLLWETSLLDPEEGIRFRGLSIPECQKVLPTAQSGAEPLPEGLLWL 129
Query: 440 SSLAIYPPKRK*SAV*R-MGGEGGYRLT*XQC*TXCPANCIPCRSSRLPSPHSTVNLNXL 616
P K + A+ + + P+ P V
Sbjct: 130 LLTGKVPSKEQVEALSKDLANRAAVPDYVYNAIDALPSTAHPMTQFASGVMALQVQSEFQ 189
Query: 617 KPTQXGVHKSQYWEYVYXDTM 679
K + G+HKS++WE Y D +
Sbjct: 190 KAYENGIHKSKFWEPTYEDCL 210
Score = 60.1 bits (139), Expect = 1e-09
Identities = 27/70 (38%), Positives = 43/70 (61%), Gaps = 1/70 (1%)
Frame = +1
Query: 427 LFWLLVTGDIPTEAQVKRCLKN-GRRGRIPAHVVXMLNXMPGKLHPMSQFSAAVTALNSE 603
L WLL+TG +P++ QV+ K+ R +P +V ++ +P HPM+QF++ V AL +
Sbjct: 126 LLWLLLTGKVPSKEQVEALSKDLANRAAVPDYVYNAIDALPSTAHPMTQFASGVMALQVQ 185
Query: 604 SKXAKAYSXG 633
S+ KAY G
Sbjct: 186 SEFQKAYENG 195
Score = 33.5 bits (73), Expect = 0.13
Identities = 12/34 (35%), Positives = 25/34 (73%)
Frame = +3
Query: 153 SAEQTNLKSILQEKIPKEQEKIREFRKKHGSTKV 254
S+ +LKS LQE IP++Q+++++ + +HG ++
Sbjct: 34 SSTDLDLKSQLQELIPEQQDRLKKLKSEHGKVQL 67
>At3g60100.1 68416.m06711 citrate synthase, mitochondrial, putative
strong similarity to SP|Q43175 Citrate synthase,
mitochondrial precursor {Solanum tuberosum}; contains
Pfam profile PF00285: Citrate synthase
Length = 433
Score = 99.5 bits (237), Expect = 2e-21
Identities = 58/141 (41%), Positives = 76/141 (53%), Gaps = 1/141 (0%)
Frame = +2
Query: 260 VTVDMMYGGMRGIKGLVWETSVLDADEGIRFRGLSIPECQQQLPKAKGGEEPLPEVFSGF 439
+TVDM+ GGMRG+ GL+WETS+LDADEGIRFRG+SIPECQ+ LP A+ GEEPLPE
Sbjct: 36 ITVDMVLGGMRGMTGLLWETSLLDADEGIRFRGMSIPECQKILPSAESGEEPLPESLLWL 95
Query: 440 SSLAIYPPKRK*SAV*RMGGEGGYRLT*XQC*TXCPANCIPCRSSRLPSPHSTVNLNXLK 619
P K + +A + E +R P+ P V K
Sbjct: 96 LLTGKVPTKEQANA---LSTELAHRAA-VPAIDALPSTAHPMTQFASGVMALQVQSEFQK 151
Query: 620 PTQXG-VHKSQYWEYVYXDTM 679
+ G + KS+YWE + D +
Sbjct: 152 AYEQGDISKSKYWEPTFEDAL 172
Score = 52.0 bits (119), Expect = 4e-07
Identities = 27/71 (38%), Positives = 39/71 (54%), Gaps = 1/71 (1%)
Frame = +1
Query: 424 SLFWLLVTGDIPTEAQVKRCLKN-GRRGRIPAHVVXMLNXMPGKLHPMSQFSAAVTALNS 600
SL WLL+TG +PT+ Q R +PA ++ +P HPM+QF++ V AL
Sbjct: 91 SLLWLLLTGKVPTKEQANALSTELAHRAAVPA-----IDALPSTAHPMTQFASGVMALQV 145
Query: 601 ESKXAKAYSXG 633
+S+ KAY G
Sbjct: 146 QSEFQKAYEQG 156
Score = 28.3 bits (60), Expect = 5.0
Identities = 10/29 (34%), Positives = 21/29 (72%)
Frame = +3
Query: 168 NLKSILQEKIPKEQEKIREFRKKHGSTKV 254
+LKS +QE IP++Q+++++ + + G V
Sbjct: 5 DLKSQMQEIIPEQQDRLKKLKSEQGKVPV 33
>At3g58740.1 68416.m06547 citrate synthase, glyoxysomal, putative
strong similarity to SP|P49299 Citrate synthase,
glyoxysomal precursor {Cucurbita maxima}; contains Pfam
profile PF00285: Citrate synthase
Length = 480
Score = 34.7 bits (76), Expect = 0.058
Identities = 16/64 (25%), Positives = 35/64 (54%), Gaps = 1/64 (1%)
Frame = +1
Query: 409 RTTTRSLFWLLVTGDIPTEAQVKRC-LKNGRRGRIPAHVVXMLNXMPGKLHPMSQFSAAV 585
++T + +LL+ G++P++ Q+ + +P V+ M+ MP +HP+ A+
Sbjct: 129 KSTYTEVTYLLIYGNLPSQRQLADWEFAISQNSAVPQGVLDMIQSMPNDVHPVGALVTAM 188
Query: 586 TALN 597
+AL+
Sbjct: 189 SALS 192
>At5g36250.1 68418.m04373 protein phosphatase 2C, putative / PP2C,
putative
Length = 448
Score = 31.9 bits (69), Expect = 0.41
Identities = 20/60 (33%), Positives = 30/60 (50%), Gaps = 3/60 (5%)
Frame = -1
Query: 360 RPRKRIPSSASSTEVSQTRPLIPRMPPYII---STVTSRL*WNHASFGIRGSSLVLWESF 190
RP KR P S SS+ + PL+ R+P + ST T L G ++++WE+F
Sbjct: 31 RPPKRRPGSCSSSFDNTEEPLLHRIPGRMFLNGSTDTVSLFSQQGKKGPNQDAMIVWENF 90
>At4g15096.1 68417.m02320 hypothetical protein
Length = 679
Score = 29.5 bits (63), Expect = 2.2
Identities = 12/39 (30%), Positives = 20/39 (51%)
Frame = +3
Query: 387 CPRLRVEKNHYPKSFLASRHWRYTHRSASKALSKEWAAR 503
C +L +++H PK LA + W + +R K L +R
Sbjct: 374 CEQLTCDESHKPKQRLAPKSWMFKYRRDHKILQSPTMSR 412
>At5g53030.2 68418.m06586 expressed protein
Length = 224
Score = 29.1 bits (62), Expect = 2.9
Identities = 14/36 (38%), Positives = 19/36 (52%)
Frame = +2
Query: 317 TSVLDADEGIRFRGLSIPECQQQLPKAKGGEEPLPE 424
T+VLD +R R LS+P + K +G P PE
Sbjct: 98 TTVLDGPYDLRRRSLSLPRSAAVIRKLRGVPAPAPE 133
>At5g53030.1 68418.m06587 expressed protein
Length = 245
Score = 29.1 bits (62), Expect = 2.9
Identities = 14/36 (38%), Positives = 19/36 (52%)
Frame = +2
Query: 317 TSVLDADEGIRFRGLSIPECQQQLPKAKGGEEPLPE 424
T+VLD +R R LS+P + K +G P PE
Sbjct: 98 TTVLDGPYDLRRRSLSLPRSAAVIRKLRGVPAPAPE 133
>At2g40955.1 68415.m05057 hypothetical protein
Length = 679
Score = 29.1 bits (62), Expect = 2.9
Identities = 12/39 (30%), Positives = 20/39 (51%)
Frame = +3
Query: 387 CPRLRVEKNHYPKSFLASRHWRYTHRSASKALSKEWAAR 503
C +L +++H PK LA + W + +R K L +R
Sbjct: 374 CEQLTCDESHKPKQRLAPKSWMFKYRRDHKILHSPTMSR 412
>At2g11270.1 68415.m01208 citrate synthase-related contains
similarity to Swiss-Prot:P20115 citrate synthase,
mitochondrial precursor [Arabidopsis thaliana]
Length = 83
Score = 28.7 bits (61), Expect = 3.8
Identities = 11/16 (68%), Positives = 14/16 (87%)
Frame = +2
Query: 260 VTVDMMYGGMRGIKGL 307
+TVDM+ GGMRG+ GL
Sbjct: 61 ITVDMVLGGMRGMTGL 76
>At4g16442.1 68417.m02489 integral membrane family protein contains
TIGRFAM TIGR01569 : plant integral membrane protein
TIGR01569; contains Pfam PF04535 : Domain of unknown
function (DUF588)
Length = 182
Score = 28.3 bits (60), Expect = 5.0
Identities = 12/38 (31%), Positives = 20/38 (52%)
Frame = +3
Query: 264 QLI*CTVACVESKVWFGKPLCWMPMKESVSVVYPSLSA 377
Q + C V ++ KV F KPL W ++ Y +++A
Sbjct: 71 QSVRCVVGTMKGKVLFSKPLAWAFFSGDQAMAYLNVAA 108
>At4g11560.1 68417.m01853 bromo-adjacent homology (BAH)
domain-containing protein contains Pfam profile PF01426:
BAH domain
Length = 587
Score = 27.9 bits (59), Expect = 6.6
Identities = 10/31 (32%), Positives = 18/31 (58%)
Frame = +1
Query: 394 G*GWRRTTTRSLFWLLVTGDIPTEAQVKRCL 486
G W+ + TR LF+ ++P E+ + RC+
Sbjct: 182 GGNWQSSDTRELFYSFHRDEVPAESVMHRCV 212
>At3g15750.1 68416.m01995 expressed protein
Length = 186
Score = 27.9 bits (59), Expect = 6.6
Identities = 14/36 (38%), Positives = 22/36 (61%)
Frame = +3
Query: 138 LLRGLSAEQTNLKSILQEKIPKEQEKIREFRKKHGS 245
L+RG+S+ L L+EK+ +QE +F+K H S
Sbjct: 79 LVRGVSSALAFLPDELREKLIDKQETREKFQKLHSS 114
>At1g59650.1 68414.m06709 expressed protein ; supporting cDNA
gi|6520232|dbj|AB028233.1|
Length = 492
Score = 27.5 bits (58), Expect = 8.8
Identities = 10/27 (37%), Positives = 17/27 (62%)
Frame = +2
Query: 545 PANCIPCRSSRLPSPHSTVNLNXLKPT 625
P+NC+PC +S +PS +L+ P+
Sbjct: 159 PSNCLPCLNSTVPSIEKRRSLSSSPPS 185
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 14,323,323
Number of Sequences: 28952
Number of extensions: 283820
Number of successful extensions: 944
Number of sequences better than 10.0: 14
Number of HSP's better than 10.0 without gapping: 908
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 941
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1447936096
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
- SilkBase 1999-2023 -