BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= NV060485.seq (686 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBC106.06 |cct4||chaperonin-containing T-complex delta subunit ... 124 1e-29 SPAC1420.02c |cct5||chaperonin-containing T-complex epsilon subu... 109 5e-25 SPBC1A4.08c |cct3||chaperonin-containing T-complex gamma subunit... 95 1e-20 SPBC25H2.12c |cct7||chaperonin-containing T-complex eta subunit ... 94 1e-20 SPBC12D12.03 |cct1||chaperonin-containing T-complex alpha subuni... 91 1e-19 SPAC1D4.04 |cct2||chaperonin-containing T-complex beta subunit C... 85 9e-18 SPBC337.05c |cct8||chaperonin-containing T-complex theta subunit... 78 1e-15 SPBC646.11 |cct6||chaperonin-containing T-complex zeta subunit C... 75 1e-14 SPAC12G12.04 |hsp60|hsp60|mitochondrial heat shock protein Hsp60... 37 0.003 SPAC10F6.12c |mam4||protein-S isoprenylcysteine O-methyltransfer... 27 1.9 SPAC22F3.06c |lon1||Lon protease homolog Lon1|Schizosaccharomyce... 26 4.4 SPBC1773.09c |mug184||meiotically upregulated gene Mug184|Schizo... 26 5.9 SPCC1322.05c |||leukotriene A-4 hydrolase |Schizosaccharomyces p... 25 7.8 SPAC19A8.05c |vps27|sst4|sorting receptor for ubiquitinated memb... 25 7.8 SPAC821.05 |||translation initiation factor eIF3h|Schizosaccharo... 25 7.8 SPAC31A2.06 |||conserved fungal protein|Schizosaccharomyces pomb... 25 7.8 >SPBC106.06 |cct4||chaperonin-containing T-complex delta subunit Cct4|Schizosaccharomyces pombe|chr 2|||Manual Length = 527 Score = 124 bits (299), Expect = 1e-29 Identities = 70/144 (48%), Positives = 92/144 (63%) Frame = +2 Query: 254 KVTITNDGATILKQMSVIHPAAKMLVELSRAQDIEAGDGTTSVVVIAGALLDSAEKLLQK 433 +V +TNDGATILK +SV+HPAAKMLV+LS AQD+EAGDGTTSVV++AG++L AEKLL+K Sbjct: 54 EVILTNDGATILKHLSVLHPAAKMLVDLSAAQDVEAGDGTTSVVILAGSMLACAEKLLKK 113 Query: 434 GIHPTVISDGFQKALQLALQVVENMQLQWT*IMKMLCSRQLQHH*IPK*FHNTQLFWAPI 613 GIHPTVI++ FQ+A + ++ L + R K API Sbjct: 114 GIHPTVIAESFQRAAGFTVDCMKENALAIELSDRESLLRAATTSLNSKIVSQYSNLLAPI 173 Query: 614 AVQAIRAVMEPIVSGVGARVDLRD 685 AV A+ V++P V VDL+D Sbjct: 174 AVDAVLKVIDP---RVATNVDLKD 194 Score = 68.1 bits (159), Expect = 1e-12 Identities = 29/45 (64%), Positives = 39/45 (86%) Frame = +3 Query: 126 YKDKSKPTDIRLSNINAAKAVADAIRTSLGPRGMDKMIQAANGKL 260 ++D+ KP ++RLSNI AA++VADAIRTSLGP+GMDKMIQ G++ Sbjct: 11 FQDREKPQEVRLSNIMAARSVADAIRTSLGPKGMDKMIQTGKGEV 55 >SPAC1420.02c |cct5||chaperonin-containing T-complex epsilon subunit Cct5|Schizosaccharomyces pombe|chr 1|||Manual Length = 546 Score = 109 bits (261), Expect = 5e-25 Identities = 51/84 (60%), Positives = 67/84 (79%) Frame = +2 Query: 242 SSQWKVTITNDGATILKQMSVIHPAAKMLVELSRAQDIEAGDGTTSVVVIAGALLDSAEK 421 S ++T+TNDGATIL QM V H AK+LV+LS++QD E GDGTT VVV+AGALL+ AE Sbjct: 65 SPDGEITVTNDGATILDQMEVEHQIAKLLVQLSKSQDDEIGDGTTGVVVLAGALLEQAEA 124 Query: 422 LLQKGIHPTVISDGFQKALQLALQ 493 L+ KGIHP I+DG++KA Q+A++ Sbjct: 125 LIDKGIHPIRIADGYEKACQVAVK 148 Score = 40.7 bits (91), Expect = 2e-04 Identities = 17/33 (51%), Positives = 27/33 (81%) Frame = +3 Query: 162 SNINAAKAVADAIRTSLGPRGMDKMIQAANGKL 260 S+I A K VA+ +RTSLGPRG+DK++ + +G++ Sbjct: 38 SHILATKTVANIVRTSLGPRGLDKILISPDGEI 70 >SPBC1A4.08c |cct3||chaperonin-containing T-complex gamma subunit Cct3|Schizosaccharomyces pombe|chr 2|||Manual Length = 528 Score = 94.7 bits (225), Expect = 1e-20 Identities = 42/86 (48%), Positives = 63/86 (73%) Frame = +2 Query: 257 VTITNDGATILKQMSVIHPAAKMLVELSRAQDIEAGDGTTSVVVIAGALLDSAEKLLQKG 436 V +TNDG IL+++ V HPAAK ++EL+R QD E GDGTTSV+++AG +L +A LL + Sbjct: 55 VLLTNDGHAILREIEVAHPAAKSMIELARTQDEEVGDGTTSVIILAGEILAAASPLLDRK 114 Query: 437 IHPTVISDGFQKALQLALQVVENMQL 514 IHP V+ F++AL+ AL +++ + L Sbjct: 115 IHPVVMIRSFKQALEDALSIIDEITL 140 Score = 42.7 bits (96), Expect = 5e-05 Identities = 20/28 (71%), Positives = 23/28 (82%) Frame = +3 Query: 156 RLSNINAAKAVADAIRTSLGPRGMDKMI 239 ++SNI AAKAVAD IRT LGPR M KM+ Sbjct: 21 QMSNIQAAKAVADVIRTCLGPRAMLKML 48 >SPBC25H2.12c |cct7||chaperonin-containing T-complex eta subunit Cct7|Schizosaccharomyces pombe|chr 2|||Manual Length = 558 Score = 94.3 bits (224), Expect = 1e-20 Identities = 43/87 (49%), Positives = 63/87 (72%) Frame = +2 Query: 254 KVTITNDGATILKQMSVIHPAAKMLVELSRAQDIEAGDGTTSVVVIAGALLDSAEKLLQK 433 +V I+NDGATI+K + ++HPAAK LV+++RAQD E GDGTTSVVV AG LL A ++ Sbjct: 59 EVVISNDGATIMKLLDIVHPAAKTLVDIARAQDAEVGDGTTSVVVFAGELLREARTFVED 118 Query: 434 GIHPTVISDGFQKALQLALQVVENMQL 514 G+ +I G++KA QLA+ ++ + + Sbjct: 119 GVSSHLIIRGYRKAAQLAVNKIKEIAI 145 Score = 40.7 bits (91), Expect = 2e-04 Identities = 21/43 (48%), Positives = 27/43 (62%) Frame = +3 Query: 132 DKSKPTDIRLSNINAAKAVADAIRTSLGPRGMDKMIQAANGKL 260 D S+ LSNINA AV D IRT+LGP G DK++ G++ Sbjct: 18 DDSQGRGQLLSNINACVAVQDTIRTTLGPLGADKLMVDDRGEV 60 >SPBC12D12.03 |cct1||chaperonin-containing T-complex alpha subunit Cct1|Schizosaccharomyces pombe|chr 2|||Manual Length = 556 Score = 91.5 bits (217), Expect = 1e-19 Identities = 44/84 (52%), Positives = 62/84 (73%) Frame = +2 Query: 257 VTITNDGATILKQMSVIHPAAKMLVELSRAQDIEAGDGTTSVVVIAGALLDSAEKLLQKG 436 VT+TNDGATIL + V HPA K+LVEL++ QD E GDGTTSVV+IA LL A +L++ Sbjct: 57 VTVTNDGATILSLLDVEHPAGKVLVELAQQQDKEVGDGTTSVVIIAAELLRRANELVKNK 116 Query: 437 IHPTVISDGFQKALQLALQVVENM 508 IHPT I G++ A++ A++ + ++ Sbjct: 117 IHPTTIITGYRLAIREAVKFMTDV 140 Score = 37.9 bits (84), Expect = 0.001 Identities = 15/36 (41%), Positives = 25/36 (69%) Frame = +3 Query: 132 DKSKPTDIRLSNINAAKAVADAIRTSLGPRGMDKMI 239 +K D+R N+ A A+A+ +++SLGP G+DKM+ Sbjct: 15 EKISGEDVRNQNVLATTAIANVVKSSLGPVGLDKML 50 >SPAC1D4.04 |cct2||chaperonin-containing T-complex beta subunit Cct2|Schizosaccharomyces pombe|chr 1|||Manual Length = 527 Score = 85.0 bits (201), Expect = 9e-18 Identities = 42/87 (48%), Positives = 59/87 (67%) Frame = +2 Query: 239 SSSQWKVTITNDGATILKQMSVIHPAAKMLVELSRAQDIEAGDGTTSVVVIAGALLDSAE 418 S+S + +TNDGATILK +++ + AAK+LV +S+ QD E GDGTTSV V A LL AE Sbjct: 51 SNSSGDIVVTNDGATILKSIALDNAAAKVLVNISKVQDDEVGDGTTSVCVFAAELLRQAE 110 Query: 419 KLLQKGIHPTVISDGFQKALQLALQVV 499 ++ IHP VI DG++ A + A+ + Sbjct: 111 IMVNAKIHPQVIIDGYRIATKTAIDAL 137 Score = 37.1 bits (82), Expect = 0.002 Identities = 15/30 (50%), Positives = 24/30 (80%) Frame = +3 Query: 156 RLSNINAAKAVADAIRTSLGPRGMDKMIQA 245 RLS+ A AV D ++++LGP+GMDK++Q+ Sbjct: 22 RLSSFVGAIAVGDLVKSTLGPKGMDKILQS 51 >SPBC337.05c |cct8||chaperonin-containing T-complex theta subunit Cct8 |Schizosaccharomyces pombe|chr 2|||Manual Length = 546 Score = 77.8 bits (183), Expect = 1e-15 Identities = 34/82 (41%), Positives = 56/82 (68%) Frame = +2 Query: 263 ITNDGATILKQMSVIHPAAKMLVELSRAQDIEAGDGTTSVVVIAGALLDSAEKLLQKGIH 442 +TND ATI++++ VIHPAAK++V+ ++ Q+ E GD VVV G LL AE +++ G+ Sbjct: 64 LTNDAATIIRELEVIHPAAKLVVDATQQQENELGDAANFVVVFTGELLAKAENMIRMGLT 123 Query: 443 PTVISDGFQKALQLALQVVENM 508 P I+ G++ AL ++V+E + Sbjct: 124 PLEIAKGYEMALSHTMEVLEEI 145 Score = 33.1 bits (72), Expect = 0.039 Identities = 17/55 (30%), Positives = 28/55 (50%) Frame = +3 Query: 75 IMAPKAGGDAIKANSSVYKDKSKPTDIRLSNINAAKAVADAIRTSLGPRGMDKMI 239 + PKA G + Y+ D + N NA + +++ RTSLGP G +K++ Sbjct: 3 LRVPKASGPQLFREG--YRIMQGVEDAVIRNCNAIRELSEITRTSLGPNGKNKIV 55 >SPBC646.11 |cct6||chaperonin-containing T-complex zeta subunit Cct6|Schizosaccharomyces pombe|chr 2|||Manual Length = 535 Score = 74.5 bits (175), Expect = 1e-14 Identities = 33/85 (38%), Positives = 54/85 (63%) Frame = +2 Query: 257 VTITNDGATILKQMSVIHPAAKMLVELSRAQDIEAGDGTTSVVVIAGALLDSAEKLLQKG 436 + +T DG +L +M + +P A + + + AQD GDGTTSV ++ G LL AE +++G Sbjct: 52 IKLTKDGKVLLTEMQIQNPTASCIAKAATAQDDATGDGTTSVCLLVGELLKQAELYIREG 111 Query: 437 IHPTVISDGFQKALQLALQVVENMQ 511 +HP++ISDGF A AL +++ + Sbjct: 112 LHPSLISDGFNLAKNEALTFLDSFK 136 Score = 29.1 bits (62), Expect = 0.63 Identities = 12/33 (36%), Positives = 21/33 (63%) Frame = +3 Query: 165 NINAAKAVADAIRTSLGPRGMDKMIQAANGKLQ 263 NI+AA + D ++++LGP G KM+ G ++ Sbjct: 21 NISAAIGLQDVLKSNLGPTGTTKMLVDGAGAIK 53 >SPAC12G12.04 |hsp60|hsp60|mitochondrial heat shock protein Hsp60|Schizosaccharomyces pombe|chr 1|||Manual Length = 582 Score = 36.7 bits (81), Expect = 0.003 Identities = 20/84 (23%), Positives = 43/84 (51%), Gaps = 4/84 (4%) Frame = +2 Query: 263 ITNDGATILKQMSVIHP----AAKMLVELSRAQDIEAGDGTTSVVVIAGALLDSAEKLLQ 430 IT DG T+ + +S+ A+++ +++ + AGDGTT+ V+ A+ + + Sbjct: 80 ITKDGVTVARSVSLKDKFENLGARLVQDVASKTNEVAGDGTTTATVLTRAIFSETVRNVA 139 Query: 431 KGIHPTVISDGFQKALQLALQVVE 502 G +P + G Q A+ ++ ++ Sbjct: 140 AGCNPMDLRRGIQLAVDNVVEFLQ 163 >SPAC10F6.12c |mam4||protein-S isoprenylcysteine O-methyltransferase Mam4|Schizosaccharomyces pombe|chr 1|||Manual Length = 236 Score = 27.5 bits (58), Expect = 1.9 Identities = 14/38 (36%), Positives = 21/38 (55%) Frame = +1 Query: 298 ECYSSSCKNVGRIISCSRY*SW*WNNISSCYCWCVVGF 411 EC S K+ ++I+C R+ S+ N I S Y +GF Sbjct: 87 ECLLSGGKSFAKVINCLRFPSFLINFIFSVYQTSALGF 124 >SPAC22F3.06c |lon1||Lon protease homolog Lon1|Schizosaccharomyces pombe|chr 1|||Manual Length = 1067 Score = 26.2 bits (55), Expect = 4.4 Identities = 21/87 (24%), Positives = 40/87 (45%), Gaps = 2/87 (2%) Frame = +2 Query: 263 ITNDGATILKQMSVIHPAAK-MLVELSRAQDI-EAGDGTTSVVVIAGALLDSAEKLLQKG 436 +T++ + K ++ + P + + S +Q D + A A+ + + LQ+ Sbjct: 345 VTSEIMNVFKDIANVSPLFREQIANFSISQTSGNVFDEPAKLADFAAAVSAADHRELQEV 404 Query: 437 IHPTVISDGFQKALQLALQVVENMQLQ 517 + T I D QKAL + + + N QLQ Sbjct: 405 LEATNIGDRLQKALYVLKKELLNAQLQ 431 >SPBC1773.09c |mug184||meiotically upregulated gene Mug184|Schizosaccharomyces pombe|chr 2|||Manual Length = 551 Score = 25.8 bits (54), Expect = 5.9 Identities = 11/16 (68%), Positives = 11/16 (68%) Frame = +3 Query: 555 CNITKFQSSFTTLNYF 602 CNI KF SSF T N F Sbjct: 276 CNIPKFNSSFKTSNDF 291 >SPCC1322.05c |||leukotriene A-4 hydrolase |Schizosaccharomyces pombe|chr 3|||Manual Length = 612 Score = 25.4 bits (53), Expect = 7.8 Identities = 9/35 (25%), Positives = 19/35 (54%) Frame = +2 Query: 203 HKLRASWNG*DDSSSQWKVTITNDGATILKQMSVI 307 H+L SW+G ++ W+ N+G T+ + ++ Sbjct: 294 HELAHSWSGNLVTNESWQCFWLNEGMTVFLERKIL 328 >SPAC19A8.05c |vps27|sst4|sorting receptor for ubiquitinated membrane proteins, ESCRT 0 complex|Schizosaccharomyces pombe|chr 1|||Manual Length = 610 Score = 25.4 bits (53), Expect = 7.8 Identities = 10/20 (50%), Positives = 12/20 (60%), Gaps = 2/20 (10%) Frame = -2 Query: 658 TTNNRFHYCSNC--LHCNGC 605 T NR H+C NC + CN C Sbjct: 184 TFTNRKHHCRNCGGVFCNQC 203 >SPAC821.05 |||translation initiation factor eIF3h|Schizosaccharomyces pombe|chr 1|||Manual Length = 357 Score = 25.4 bits (53), Expect = 7.8 Identities = 12/24 (50%), Positives = 15/24 (62%) Frame = -3 Query: 246 LLESSYPFHEALSLCGWHQQQLLR 175 LLES+ FH G+HQ+QL R Sbjct: 254 LLESTDEFHYEQGNLGFHQRQLAR 277 >SPAC31A2.06 |||conserved fungal protein|Schizosaccharomyces pombe|chr 1|||Manual Length = 542 Score = 25.4 bits (53), Expect = 7.8 Identities = 12/23 (52%), Positives = 14/23 (60%) Frame = -2 Query: 418 LSRIQQRTSNNNY*CCSITSFNI 350 LS Q TS NN+ C SI +F I Sbjct: 177 LSNRQLNTSENNWTCLSIENFGI 199 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,680,360 Number of Sequences: 5004 Number of extensions: 52223 Number of successful extensions: 148 Number of sequences better than 10.0: 16 Number of HSP's better than 10.0 without gapping: 133 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 147 length of database: 2,362,478 effective HSP length: 70 effective length of database: 2,012,198 effective search space used: 317927284 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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