BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= NV060481.seq (578 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC6G10.11c |ubi3||ribosomal ubiquitin fusion protein Ubi3|Schi... 151 5e-38 SPAC589.10c |||ribomal-ubiquitin fusion protein Ubi5|Schizosacch... 151 5e-38 SPAC1805.12c |uep1|ubi2|ribosomal-ubiquitin fusion protein Ubi2|... 149 2e-37 SPAC11G7.04 |ubi1||ribosomal-ubiquitin fusion protein Ubi1|Schiz... 149 2e-37 SPBC337.08c |ubi4||ubiquitin|Schizosaccharomyces pombe|chr 2|||M... 149 2e-37 SPBC12D12.08c |ned8|nedd8, ubl1, SPBC24C6.01c|ubiquitin-like pro... 96 4e-21 SPAC6G9.08 |ubp6||ubiquitin C-terminal hydrolase Ubp6|Schizosacc... 43 3e-05 SPBC2D10.12 |rhp23||Rad23 homolog Rhp23|Schizosaccharomyces pomb... 41 1e-04 SPAC26A3.16 |dph1|ucp5|UBA domain protein Dph1|Schizosaccharomyc... 41 2e-04 SPBC31E1.03 |hub1|ubl4|ubiquitin-like protein modifier Hub1|Schi... 30 0.28 SPBC1718.06 |msp1|mgm1|mitochondrial GTPase Msp1|Schizosaccharom... 28 0.86 SPAC29B12.06c |rcd1||RNA-binding protein Rcd1 |Schizosaccharomyc... 27 2.6 SPAC17C9.10 |stm1||G-protein coupled receptor Stm1|Schizosacchar... 25 6.1 SPCC1739.03 |hrr1||Helicase Required for RNAi-mediated heterochr... 25 6.1 SPAC688.10 |rev3||DNA polymerase zeta catalytic subunit Rev3|Sch... 25 8.0 >SPAC6G10.11c |ubi3||ribosomal ubiquitin fusion protein Ubi3|Schizosaccharomyces pombe|chr 1|||Manual Length = 150 Score = 151 bits (367), Expect = 5e-38 Identities = 73/78 (93%), Positives = 76/78 (97%) Frame = +1 Query: 19 MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPNQQRLIFAGKQLEDGRTLSDYN 198 MQIFVKTLTGKTITLEVE SDTI+NVK+KIQDKEGIPP+QQRLIFAGKQLEDGRTLSDYN Sbjct: 1 MQIFVKTLTGKTITLEVESSDTIDNVKSKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYN 60 Query: 199 IQKESTLHLVLRLRGGAK 252 IQKESTLHLVLRLRGG K Sbjct: 61 IQKESTLHLVLRLRGGGK 78 Score = 77.8 bits (183), Expect = 1e-15 Identities = 35/70 (50%), Positives = 46/70 (65%) Frame = +3 Query: 252 ERKKKNYSTPXXXXXXXXXXXLAVLRFYKVDENGKIHRLRRECTGEQCGAGVFMAVMEDR 431 +RKKK Y+TP LAVL++YKV+++G + RLRREC CGA FMA +DR Sbjct: 79 KRKKKTYTTPKKIKHKHKKVELAVLKYYKVEDDGSVKRLRREC--PNCGASTFMANHKDR 136 Query: 432 HYCGKCHSTM 461 YCG+CH T+ Sbjct: 137 LYCGRCHLTL 146 >SPAC589.10c |||ribomal-ubiquitin fusion protein Ubi5|Schizosaccharomyces pombe|chr 1|||Manual Length = 150 Score = 151 bits (367), Expect = 5e-38 Identities = 73/78 (93%), Positives = 76/78 (97%) Frame = +1 Query: 19 MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPNQQRLIFAGKQLEDGRTLSDYN 198 MQIFVKTLTGKTITLEVE SDTI+NVK+KIQDKEGIPP+QQRLIFAGKQLEDGRTLSDYN Sbjct: 1 MQIFVKTLTGKTITLEVESSDTIDNVKSKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYN 60 Query: 199 IQKESTLHLVLRLRGGAK 252 IQKESTLHLVLRLRGG K Sbjct: 61 IQKESTLHLVLRLRGGGK 78 Score = 77.8 bits (183), Expect = 1e-15 Identities = 35/70 (50%), Positives = 46/70 (65%) Frame = +3 Query: 252 ERKKKNYSTPXXXXXXXXXXXLAVLRFYKVDENGKIHRLRRECTGEQCGAGVFMAVMEDR 431 +RKKK Y+TP LAVL++YKV+++G + RLRREC CGA FMA +DR Sbjct: 79 KRKKKTYTTPKKIKHKHKKVELAVLKYYKVEDDGSVKRLRREC--PNCGASTFMANHKDR 136 Query: 432 HYCGKCHSTM 461 YCG+CH T+ Sbjct: 137 LYCGRCHLTL 146 >SPAC1805.12c |uep1|ubi2|ribosomal-ubiquitin fusion protein Ubi2|Schizosaccharomyces pombe|chr 1|||Manual Length = 128 Score = 149 bits (362), Expect = 2e-37 Identities = 72/76 (94%), Positives = 75/76 (98%) Frame = +1 Query: 19 MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPNQQRLIFAGKQLEDGRTLSDYN 198 MQIFVKTLTGKTITLEVE SDTI+NVK+KIQDKEGIPP+QQRLIFAGKQLEDGRTLSDYN Sbjct: 1 MQIFVKTLTGKTITLEVESSDTIDNVKSKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYN 60 Query: 199 IQKESTLHLVLRLRGG 246 IQKESTLHLVLRLRGG Sbjct: 61 IQKESTLHLVLRLRGG 76 >SPAC11G7.04 |ubi1||ribosomal-ubiquitin fusion protein Ubi1|Schizosaccharomyces pombe|chr 1|||Manual Length = 128 Score = 149 bits (362), Expect = 2e-37 Identities = 72/76 (94%), Positives = 75/76 (98%) Frame = +1 Query: 19 MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPNQQRLIFAGKQLEDGRTLSDYN 198 MQIFVKTLTGKTITLEVE SDTI+NVK+KIQDKEGIPP+QQRLIFAGKQLEDGRTLSDYN Sbjct: 1 MQIFVKTLTGKTITLEVESSDTIDNVKSKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYN 60 Query: 199 IQKESTLHLVLRLRGG 246 IQKESTLHLVLRLRGG Sbjct: 61 IQKESTLHLVLRLRGG 76 >SPBC337.08c |ubi4||ubiquitin|Schizosaccharomyces pombe|chr 2|||Manual Length = 382 Score = 149 bits (362), Expect = 2e-37 Identities = 72/76 (94%), Positives = 75/76 (98%) Frame = +1 Query: 19 MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPNQQRLIFAGKQLEDGRTLSDYN 198 MQIFVKTLTGKTITLEVE SDTI+NVK+KIQDKEGIPP+QQRLIFAGKQLEDGRTLSDYN Sbjct: 1 MQIFVKTLTGKTITLEVESSDTIDNVKSKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYN 60 Query: 199 IQKESTLHLVLRLRGG 246 IQKESTLHLVLRLRGG Sbjct: 61 IQKESTLHLVLRLRGG 76 Score = 149 bits (362), Expect = 2e-37 Identities = 72/76 (94%), Positives = 75/76 (98%) Frame = +1 Query: 19 MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPNQQRLIFAGKQLEDGRTLSDYN 198 MQIFVKTLTGKTITLEVE SDTI+NVK+KIQDKEGIPP+QQRLIFAGKQLEDGRTLSDYN Sbjct: 77 MQIFVKTLTGKTITLEVESSDTIDNVKSKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYN 136 Query: 199 IQKESTLHLVLRLRGG 246 IQKESTLHLVLRLRGG Sbjct: 137 IQKESTLHLVLRLRGG 152 Score = 149 bits (362), Expect = 2e-37 Identities = 72/76 (94%), Positives = 75/76 (98%) Frame = +1 Query: 19 MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPNQQRLIFAGKQLEDGRTLSDYN 198 MQIFVKTLTGKTITLEVE SDTI+NVK+KIQDKEGIPP+QQRLIFAGKQLEDGRTLSDYN Sbjct: 153 MQIFVKTLTGKTITLEVESSDTIDNVKSKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYN 212 Query: 199 IQKESTLHLVLRLRGG 246 IQKESTLHLVLRLRGG Sbjct: 213 IQKESTLHLVLRLRGG 228 Score = 149 bits (362), Expect = 2e-37 Identities = 72/76 (94%), Positives = 75/76 (98%) Frame = +1 Query: 19 MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPNQQRLIFAGKQLEDGRTLSDYN 198 MQIFVKTLTGKTITLEVE SDTI+NVK+KIQDKEGIPP+QQRLIFAGKQLEDGRTLSDYN Sbjct: 229 MQIFVKTLTGKTITLEVESSDTIDNVKSKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYN 288 Query: 199 IQKESTLHLVLRLRGG 246 IQKESTLHLVLRLRGG Sbjct: 289 IQKESTLHLVLRLRGG 304 Score = 149 bits (362), Expect = 2e-37 Identities = 72/76 (94%), Positives = 75/76 (98%) Frame = +1 Query: 19 MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPNQQRLIFAGKQLEDGRTLSDYN 198 MQIFVKTLTGKTITLEVE SDTI+NVK+KIQDKEGIPP+QQRLIFAGKQLEDGRTLSDYN Sbjct: 305 MQIFVKTLTGKTITLEVESSDTIDNVKSKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYN 364 Query: 199 IQKESTLHLVLRLRGG 246 IQKESTLHLVLRLRGG Sbjct: 365 IQKESTLHLVLRLRGG 380 >SPBC12D12.08c |ned8|nedd8, ubl1, SPBC24C6.01c|ubiquitin-like protein modifier Ned8|Schizosaccharomyces pombe|chr 2|||Manual Length = 78 Score = 95.9 bits (228), Expect = 4e-21 Identities = 41/77 (53%), Positives = 59/77 (76%) Frame = +1 Query: 19 MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPNQQRLIFAGKQLEDGRTLSDYN 198 M I VKTLTGK I L+++P+D + +K ++++KEGIPP+QQRLI+AGKQ+ D + Y+ Sbjct: 1 MLIKVKTLTGKEIELDIDPNDKVSRIKERVEEKEGIPPSQQRLIYAGKQMADDKNAESYH 60 Query: 199 IQKESTLHLVLRLRGGA 249 ++ S LHLVL LRGG+ Sbjct: 61 LEGGSVLHLVLALRGGS 77 >SPAC6G9.08 |ubp6||ubiquitin C-terminal hydrolase Ubp6|Schizosaccharomyces pombe|chr 1|||Manual Length = 467 Score = 43.2 bits (97), Expect = 3e-05 Identities = 20/58 (34%), Positives = 33/58 (56%) Frame = +1 Query: 46 GKTITLEVEPSDTIENVKAKIQDKEGIPPNQQRLIFAGKQLEDGRTLSDYNIQKESTL 219 GK LE+EP++T +K ++ +PP +Q++I G QL+D L I+ +TL Sbjct: 10 GKKYDLEIEPNETGSTLKHQLYSLTQVPPERQKVIVKGGQLKDDVLLGSVGIKPNATL 67 >SPBC2D10.12 |rhp23||Rad23 homolog Rhp23|Schizosaccharomyces pombe|chr 2|||Manual Length = 368 Score = 41.1 bits (92), Expect = 1e-04 Identities = 20/65 (30%), Positives = 38/65 (58%), Gaps = 1/65 (1%) Frame = +1 Query: 19 MQIFVKTLTGKTITLEVEPSDT-IENVKAKIQDKEGIPPNQQRLIFAGKQLEDGRTLSDY 195 M + K L + + +DT I +K KIQ ++ +Q+LI++G+ L D +T+ +Y Sbjct: 1 MNLTFKNLQQQKFVISDVSADTKISELKEKIQTQQNYEVERQKLIYSGRILADDKTVGEY 60 Query: 196 NIQKE 210 NI+++ Sbjct: 61 NIKEQ 65 >SPAC26A3.16 |dph1|ucp5|UBA domain protein Dph1|Schizosaccharomyces pombe|chr 1|||Manual Length = 354 Score = 40.7 bits (91), Expect = 2e-04 Identities = 21/73 (28%), Positives = 37/73 (50%) Frame = +1 Query: 19 MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPNQQRLIFAGKQLEDGRTLSDYN 198 + + +K + + V+ ++ +K I I +QRLI+AG+ L+D +L Y Sbjct: 4 ISLTIKAANDQKYAVTVDSESSVLALKEAIAPVADIEKERQRLIYAGRVLKDEESLKTYK 63 Query: 199 IQKESTLHLVLRL 237 IQ ++HLV L Sbjct: 64 IQDGHSIHLVKTL 76 >SPBC31E1.03 |hub1|ubl4|ubiquitin-like protein modifier Hub1|Schizosaccharomyces pombe|chr 2|||Manual Length = 73 Score = 29.9 bits (64), Expect = 0.28 Identities = 16/69 (23%), Positives = 31/69 (44%) Frame = +1 Query: 19 MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPNQQRLIFAGKQLEDGRTLSDYN 198 +++ GK + ++ P DT+ + K + + G P + L +D TL+DY Sbjct: 2 IEVLCNDRLGKKVRVKCMPDDTVGDFKKLVAAQTGTDPRRIVLKKWHSVFKDNITLADYE 61 Query: 199 IQKESTLHL 225 I +L + Sbjct: 62 IHDGMSLEM 70 >SPBC1718.06 |msp1|mgm1|mitochondrial GTPase Msp1|Schizosaccharomyces pombe|chr 2|||Manual Length = 903 Score = 28.3 bits (60), Expect = 0.86 Identities = 11/29 (37%), Positives = 17/29 (58%) Frame = -2 Query: 208 PSGYCSPRGYVHLPAAFQRRSTSVDWEGF 122 PS + PRG V+ P+ +RR + + GF Sbjct: 17 PSSFLKPRGLVYRPSQIRRRVSLLSLSGF 45 >SPAC29B12.06c |rcd1||RNA-binding protein Rcd1 |Schizosaccharomyces pombe|chr 1|||Manual Length = 283 Score = 26.6 bits (56), Expect = 2.6 Identities = 14/54 (25%), Positives = 24/54 (44%), Gaps = 1/54 (1%) Frame = +3 Query: 321 VLRFY-KVDENGKIHRLRRECTGEQCGAGVFMAVMEDRHYCGKCHSTMVFKDDD 479 V+R Y ++ +N + R C E F V++D H KC + ++ D Sbjct: 224 VIRCYLRLSDNPRAREALRHCLPEPLRDATFAQVLKDDHNTKKCLAQLLINLSD 277 >SPAC17C9.10 |stm1||G-protein coupled receptor Stm1|Schizosaccharomyces pombe|chr 1|||Manual Length = 271 Score = 25.4 bits (53), Expect = 6.1 Identities = 15/37 (40%), Positives = 17/37 (45%), Gaps = 6/37 (16%) Frame = +3 Query: 153 RWKAAGRWTY----PL--GLQYPEGIHPPPGVEASWW 245 RWKAA R PL EG+H P G + WW Sbjct: 99 RWKAARRIASREHEPLLQSRSLEEGLHAPIGKQQIWW 135 >SPCC1739.03 |hrr1||Helicase Required for RNAi-mediated heterochromatin assembly Hrr1|Schizosaccharomyces pombe|chr 3|||Manual Length = 1015 Score = 25.4 bits (53), Expect = 6.1 Identities = 11/24 (45%), Positives = 15/24 (62%) Frame = -1 Query: 452 VTLPAVVTVLHHRHEHSGAALLAC 381 VTL A+ T+L + H H L+AC Sbjct: 402 VTLKAIETLLENTHSHVLPILVAC 425 >SPAC688.10 |rev3||DNA polymerase zeta catalytic subunit Rev3|Schizosaccharomyces pombe|chr 1|||Manual Length = 1480 Score = 25.0 bits (52), Expect = 8.0 Identities = 12/24 (50%), Positives = 15/24 (62%) Frame = +1 Query: 130 PNQQRLIFAGKQLEDGRTLSDYNI 201 PNQ AGK+L+ R L DY+I Sbjct: 476 PNQGDTRKAGKRLKYSRNLDDYHI 499 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,124,834 Number of Sequences: 5004 Number of extensions: 40795 Number of successful extensions: 121 Number of sequences better than 10.0: 15 Number of HSP's better than 10.0 without gapping: 111 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 119 length of database: 2,362,478 effective HSP length: 69 effective length of database: 2,017,202 effective search space used: 248115846 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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