Bombyx BLAST Result against spombe

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= NV060479.seq
         (682 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPAC17H9.09c |ras1|ste5|GTPase Ras1|Schizosaccharomyces pombe|ch...    48   9e-07
SPBC428.16c |rhb1||Rheb GTPase Rhb1|Schizosaccharomyces pombe|ch...    32   0.067
SPCC297.05 |||diacylglycerol binding protein |Schizosaccharomyce...    29   0.47 
SPAC1851.04c ||SPAC27D7.01c|guanyl-nucleotide exchange factor |S...    29   0.47 
SPCC613.12c |raf1|dos1, cmc1, clr8|Rik1-associated factor Raf1|S...    29   0.47 
SPAC823.03 |ppk15||serine/threonine protein kinase Ppk15 |Schizo...    29   0.82 
SPCC63.04 |mok14||alpha-1,3-glucan synthase Mok14|Schizosaccharo...    28   1.4  
SPBC1289.10c |||transcription factor |Schizosaccharomyces pombe|...    27   1.9  
SPCPJ732.02c |||xylulose kinase |Schizosaccharomyces pombe|chr 3...    27   1.9  
SPBC21.06c |cdc7|pld1, its10|serine/threonine protein kinase Cdc...    27   2.5  
SPAC10F6.05c |ubc6||ubiquitin conjugating enzyme Ubc6|Schizosacc...    26   4.4  
SPCC1494.07 |||conserved eukaryotic protein|Schizosaccharomyces ...    25   7.7  
SPCC777.12c |||sequence orphan|Schizosaccharomyces pombe|chr 3||...    25   7.7  

>SPAC17H9.09c |ras1|ste5|GTPase Ras1|Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 219

 Score = 48.4 bits (110), Expect = 9e-07
 Identities = 21/51 (41%), Positives = 31/51 (60%)
 Frame = +2

Query: 287 LRSTIMREFXXXXXXXXXXXXXALTVQFVSXCFMEKYDPTIEDFYRKEIEV 439
           +RST +RE+             ALT+Q +   F+++YDPTIED YRK+ E+
Sbjct: 1   MRSTYLREYKLVVVGDGGVGKSALTIQLIQSHFVDEYDPTIEDSYRKKCEI 51


>SPBC428.16c |rhb1||Rheb GTPase Rhb1|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 185

 Score = 32.3 bits (70), Expect = 0.067
 Identities = 14/28 (50%), Positives = 20/28 (71%)
 Frame = +2

Query: 353 ALTVQFVSXCFMEKYDPTIEDFYRKEIE 436
           +LTVQ+V   F+E Y PTIE+ + K I+
Sbjct: 21  SLTVQYVENHFVESYYPTIENTFSKNIK 48


>SPCC297.05 |||diacylglycerol binding protein |Schizosaccharomyces
           pombe|chr 3|||Manual
          Length = 973

 Score = 29.5 bits (63), Expect = 0.47
 Identities = 14/47 (29%), Positives = 26/47 (55%)
 Frame = -1

Query: 451 KENGHFDFFSVEILYRRIIFFHKTS*YKLHSQRTLSDPTRTQHDHFK 311
           K+   FD  SV  L+RR +  + ++  K HS++  S+P+    +H +
Sbjct: 99  KKKDSFDHASVTSLHRRSLSSNTSTPRKYHSRKPYSEPSVIDKEHLQ 145


>SPAC1851.04c ||SPAC27D7.01c|guanyl-nucleotide exchange factor
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 1052

 Score = 29.5 bits (63), Expect = 0.47
 Identities = 17/61 (27%), Positives = 31/61 (50%), Gaps = 3/61 (4%)
 Frame = -1

Query: 328 QHDHFKFAHYCAP*ITIASLPH---NVMKRIHTVRYKMSLLCWFMTHSDAITELHLLKLI 158
           Q D   +A   AP I +++L     N + ++ + +Y +  + WF+ HS  I+ L+    I
Sbjct: 141 QRDSIVYAADSAPIIQVSTLETTDPNKLWKVSSKQYDLREVTWFLDHSSLISRLYFDVKI 200

Query: 157 D 155
           D
Sbjct: 201 D 201


>SPCC613.12c |raf1|dos1, cmc1, clr8|Rik1-associated factor
           Raf1|Schizosaccharomyces pombe|chr 3|||Manual
          Length = 638

 Score = 29.5 bits (63), Expect = 0.47
 Identities = 17/43 (39%), Positives = 22/43 (51%), Gaps = 1/43 (2%)
 Frame = -3

Query: 134 FHVN*NNSNCIALITLKTNA-EHNFHSTDVINTSLVPHEQQVG 9
           FH + NN  C+AL    T +    F++ D   TSL  H  QVG
Sbjct: 416 FHTS-NNRGCLALFDASTASFVQKFNTADEAFTSLYMHPSQVG 457


>SPAC823.03 |ppk15||serine/threonine protein kinase Ppk15
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 534

 Score = 28.7 bits (61), Expect = 0.82
 Identities = 12/24 (50%), Positives = 16/24 (66%)
 Frame = +1

Query: 487 QLLRCEILLKXARLVXYSLQPTNV 558
           QLL C  LLK AR++   L+P N+
Sbjct: 240 QLLSCTSLLKQARIIHCDLKPENI 263


>SPCC63.04 |mok14||alpha-1,3-glucan synthase
           Mok14|Schizosaccharomyces pombe|chr 3|||Manual
          Length = 1369

 Score = 27.9 bits (59), Expect = 1.4
 Identities = 17/68 (25%), Positives = 32/68 (47%)
 Frame = -3

Query: 635 VSSALFHSEWTVDLDRVINPSWFVSETLVGCNEYXTSLAXFNKISHRSNCSVPAVSKISK 456
           +SSA+F+S W   +  +I         +   N+Y  +LA    +     C++   +  ++
Sbjct: 362 ISSAIFYSVWNQSIAAIIRRE---KVDIYHINDYHGALAPIYNLPEVIPCAISLHN--AE 416

Query: 455 TQGEWPLR 432
            QG WPL+
Sbjct: 417 FQGLWPLQ 424


>SPBC1289.10c |||transcription factor |Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 743

 Score = 27.5 bits (58), Expect = 1.9
 Identities = 11/20 (55%), Positives = 14/20 (70%)
 Frame = -3

Query: 362 QSTHSFRPHPNPTRPL*IRA 303
           Q THS RP PN + PL +R+
Sbjct: 589 QQTHSSRPTPNASSPLDVRS 608


>SPCPJ732.02c |||xylulose kinase |Schizosaccharomyces pombe|chr
           3|||Manual
          Length = 555

 Score = 27.5 bits (58), Expect = 1.9
 Identities = 10/28 (35%), Positives = 15/28 (53%)
 Frame = -1

Query: 670 APNWEDASEALVCQAPYSTVNGPSTLTE 587
           +PNW+DAS    C+   S + G   L +
Sbjct: 122 SPNWQDASTTKECEELESCIGGAKALAD 149


>SPBC21.06c |cdc7|pld1, its10|serine/threonine protein kinase
            Cdc7|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 1062

 Score = 27.1 bits (57), Expect = 2.5
 Identities = 12/39 (30%), Positives = 22/39 (56%)
 Frame = -3

Query: 326  TRPL*IRALLCSVNHNCESSTQCYETYSHCAL*NVIAML 210
            T+P+ +  +   +   CE+S+Q     +HC L +V+A L
Sbjct: 972  TKPIVVLNIFQIMKSMCEASSQSVAYIAHCGLPDVVANL 1010


>SPAC10F6.05c |ubc6||ubiquitin conjugating enzyme
           Ubc6|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 227

 Score = 26.2 bits (55), Expect = 4.4
 Identities = 11/19 (57%), Positives = 12/19 (63%)
 Frame = -3

Query: 581 NPSWFVSETLVGCNEYXTS 525
           NPSW VS  LVG   + TS
Sbjct: 99  NPSWMVSTILVGLVSFMTS 117


>SPCC1494.07 |||conserved eukaryotic protein|Schizosaccharomyces
            pombe|chr 3|||Manual
          Length = 1502

 Score = 25.4 bits (53), Expect = 7.7
 Identities = 19/75 (25%), Positives = 33/75 (44%), Gaps = 3/75 (4%)
 Frame = +2

Query: 230  IAHSVNTFHNIVWKTRNCDLR---STIMREFXXXXXXXXXXXXXALTVQFVSXCFMEKYD 400
            IA S+  F + +W  RNC +    + I R F                +   +  F  K+ 
Sbjct: 855  IALSIEGFSHELWPIRNCSVMLFTALINRAFGSKKPKDAVNLGNNKGLS--TKMFFSKF- 911

Query: 401  PTIEDFYRKEIEVAI 445
            PT+ D+  +E+EV++
Sbjct: 912  PTLHDYLLRELEVSV 926


>SPCC777.12c |||sequence orphan|Schizosaccharomyces pombe|chr
           3|||Manual
          Length = 238

 Score = 25.4 bits (53), Expect = 7.7
 Identities = 15/42 (35%), Positives = 17/42 (40%), Gaps = 1/42 (2%)
 Frame = -3

Query: 593 DRVINPS-WFVSETLVGCNEYXTSLAXFNKISHRSNCSVPAV 471
           D VI    WFV  T  GC         FN +SH  +   P V
Sbjct: 65  DHVIESGKWFVQLTAEGCANCTLGELLFNDLSHAFHEKYPDV 106


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,861,781
Number of Sequences: 5004
Number of extensions: 55743
Number of successful extensions: 127
Number of sequences better than 10.0: 13
Number of HSP's better than 10.0 without gapping: 125
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 127
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 313902888
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)