Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= NV060475.seq
(646 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC1834.01 |sup45||translation release factor eRF1|Schizosaccha... 128 5e-31
SPCC18B5.06 |erf1|sup45|translation release factor eRF1|Schizosa... 38 0.002
SPCC550.02c |cwf5|ecm2|RNA-binding protein Cwf5|Schizosaccharomy... 28 1.3
SPCC1235.11 |||conserved eukaryotic protein|Schizosaccharomyces ... 27 1.8
SPAC19B12.01 ||SPAC4F10.21|TPR repeat protein, TTC27 family|Schi... 27 1.8
SPBC646.17c |dic1|SPBC855.01c, SPBP35G2.01c, mug44|dynein interm... 27 3.1
SPBC428.11 |||homocysteine synthase |Schizosaccharomyces pombe|c... 27 3.1
SPCC297.05 |||diacylglycerol binding protein |Schizosaccharomyce... 26 4.0
SPAC24H6.13 |||DUF221 family protein|Schizosaccharomyces pombe|c... 25 7.1
>SPAC1834.01 |sup45||translation release factor
eRF1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 433
Score = 128 bits (310), Expect = 5e-31
Identities = 59/83 (71%), Positives = 72/83 (86%)
Frame = +2
Query: 5 TRLSQSDMFDPRLQAKIIKLVDVSYGGENGFNQAIELAAESLQNVKFIQEKKLIGRYFDE 184
T L QSD+FD RLQ++IIK VDVSYGG+ GFNQAIELAA++L NVK++QEKKLI R+FDE
Sbjct: 231 TELGQSDLFDQRLQSRIIKTVDVSYGGDAGFNQAIELAADTLSNVKYVQEKKLIQRFFDE 290
Query: 185 ISQDTGKYCFGVDDTLRALELGS 253
IS D+GKYCFGV DT+ AL+ G+
Sbjct: 291 ISLDSGKYCFGVVDTMNALQEGA 313
Score = 69.3 bits (162), Expect = 4e-13
Identities = 41/89 (46%), Positives = 55/89 (61%), Gaps = 4/89 (4%)
Frame = +1
Query: 256 ETLICWENLDIQRYVLKSHATNQETILHLTPEQE-KDK--SHFTDKESGVELELVECQPL 426
ETL+C+ +LD+ RY K+ N I ++T EQE KD S DK++G E+ELV L
Sbjct: 315 ETLLCFADLDMIRYEFKNSEGNP-VITYMTKEQEEKDSTNSFLLDKDTGAEMELVSSMLL 373
Query: 427 LEWLANNYKSFGATLESSRTRAR-GKQFV 510
EWLA +YK +GA LE R++ G QFV
Sbjct: 374 SEWLAEHYKDYGANLEFVSDRSQEGMQFV 402
>SPCC18B5.06 |erf1|sup45|translation release factor
eRF1|Schizosaccharomyces pombe|chr 3|||Manual
Length = 390
Score = 37.5 bits (83), Expect = 0.002
Identities = 19/62 (30%), Positives = 39/62 (62%), Gaps = 2/62 (3%)
Frame = +2
Query: 74 SYGGENGFNQAI-ELAAES-LQNVKFIQEKKLIGRYFDEISQDTGKYCFGVDDTLRALEL 247
S G + N+ + + A ES L + K++QE +++ +++D +++D K +G + L+A EL
Sbjct: 246 STGHIHSLNEILKDPAVESKLADTKYVQEIRVLNKFYDVMNEDDRKAWYGPNHVLKAFEL 305
Query: 248 GS 253
G+
Sbjct: 306 GA 307
>SPCC550.02c |cwf5|ecm2|RNA-binding protein Cwf5|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 354
Score = 27.9 bits (59), Expect = 1.3
Identities = 13/33 (39%), Positives = 20/33 (60%)
Frame = -1
Query: 577 FPVVSCIDGSHFVRRNRHPSKPRRTASLWLLSV 479
FPV I+G+ + N P K ++ ASL+L+ V
Sbjct: 187 FPVKKIINGNVSLSINMEPPKDKKIASLFLIGV 219
>SPCC1235.11 |||conserved eukaryotic protein|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 141
Score = 27.5 bits (58), Expect = 1.8
Identities = 10/27 (37%), Positives = 15/27 (55%)
Frame = -3
Query: 356 SCSGVRWRIVSWLVACDFNTYLCMSRF 276
+ V R ++WL + DF YLC + F
Sbjct: 11 AAQSVTRRFITWLKSPDFRKYLCSTHF 37
>SPAC19B12.01 ||SPAC4F10.21|TPR repeat protein, TTC27
family|Schizosaccharomyces pombe|chr 1|||Manual
Length = 817
Score = 27.5 bits (58), Expect = 1.8
Identities = 18/54 (33%), Positives = 26/54 (48%)
Frame = +3
Query: 198 QESIASVSMIHCALSNSDPRDVDLLGEPRHTKIRIEVACHQPGNNSPPDA*TRE 359
Q+S+A + LS+ DP L +P + + CH NN+P D TRE
Sbjct: 277 QKSLAPSEELDPILSSEDPNHPSKL-DPLDASLLLAF-CHIIKNNNPDDGLTRE 328
>SPBC646.17c |dic1|SPBC855.01c, SPBP35G2.01c, mug44|dynein
intermediate chain Dic1|Schizosaccharomyces pombe|chr
2|||Manual
Length = 544
Score = 26.6 bits (56), Expect = 3.1
Identities = 13/39 (33%), Positives = 23/39 (58%)
Frame = +2
Query: 44 QAKIIKLVDVSYGGENGFNQAIELAAESLQNVKFIQEKK 160
+ K ++ +VSY G N F I++ + QNV F+++ K
Sbjct: 379 ETKAVQPSNVSYEGHNVFISGIDVMTSNSQNV-FLEKNK 416
>SPBC428.11 |||homocysteine synthase |Schizosaccharomyces pombe|chr
2|||Manual
Length = 429
Score = 26.6 bits (56), Expect = 3.1
Identities = 14/26 (53%), Positives = 17/26 (65%)
Frame = +2
Query: 476 HHGQEPEGSSSSRLRGMAVPPYKVTS 553
H GQEP+ ++SSR AVP Y TS
Sbjct: 14 HAGQEPDAATSSR----AVPIYATTS 35
>SPCC297.05 |||diacylglycerol binding protein |Schizosaccharomyces
pombe|chr 3|||Manual
Length = 973
Score = 26.2 bits (55), Expect = 4.0
Identities = 11/30 (36%), Positives = 14/30 (46%)
Frame = -3
Query: 311 CDFNTYLCMSRFSQQINVSRIRVRERAVYH 222
CDF Y+ M F I + VR + YH
Sbjct: 207 CDFPNYVPMFAFPNDITIKESDVRPVSTYH 236
>SPAC24H6.13 |||DUF221 family protein|Schizosaccharomyces pombe|chr
1|||Manual
Length = 871
Score = 25.4 bits (53), Expect = 7.1
Identities = 11/26 (42%), Positives = 13/26 (50%)
Frame = +1
Query: 265 ICWENLDIQRYVLKSHATNQETILHL 342
I W NLD+ Y + T TIL L
Sbjct: 359 IVWSNLDLSMYTRRGKKTISNTILTL 384
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,779,243
Number of Sequences: 5004
Number of extensions: 56722
Number of successful extensions: 178
Number of sequences better than 10.0: 9
Number of HSP's better than 10.0 without gapping: 172
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 177
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 289756512
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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