Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= NV060475.seq
(646 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At3g26618.1 68416.m03325 eukaryotic release factor 1 family prot... 146 1e-35
At1g12920.1 68414.m01500 eukaryotic release factor 1 family prot... 146 1e-35
At5g47880.1 68418.m05915 eukaryotic peptide chain release factor... 142 1e-34
At5g19310.1 68418.m02301 homeotic gene regulator, putative simil... 33 0.21
At3g19470.1 68416.m02468 F-box family protein contains F-box dom... 29 2.6
At3g49350.1 68416.m05395 RabGAP/TBC domain-containing protein si... 29 3.5
At4g26610.1 68417.m03835 protein kinase, putative similar to pro... 28 4.6
At2g23740.1 68415.m02834 zinc finger (C2H2 type) family protein ... 28 4.6
At5g13300.1 68418.m01528 ARF GTPase-activating domain-containing... 28 6.1
At4g29010.1 68417.m04147 abnormal inflorescence meristem 1 / fat... 27 8.1
At3g56700.1 68416.m06307 male sterility protein, putative simila... 27 8.1
At3g16940.1 68416.m02165 calmodulin-binding protein similar to a... 27 8.1
At2g41520.2 68415.m05131 DNAJ heat shock N-terminal domain-conta... 27 8.1
At2g41520.1 68415.m05130 DNAJ heat shock N-terminal domain-conta... 27 8.1
At1g49990.1 68414.m05610 expressed protein ; expression supporte... 27 8.1
>At3g26618.1 68416.m03325 eukaryotic release factor 1 family protein
/ eRF1 family protein contains Pfam profiles: PF03463
eRF1 domain 1, PF03464 eRF1 domain 2, PF03465 eRF1
domain 3
Length = 435
Score = 146 bits (354), Expect = 1e-35
Identities = 67/83 (80%), Positives = 78/83 (93%)
Frame = +2
Query: 5 TRLSQSDMFDPRLQAKIIKLVDVSYGGENGFNQAIELAAESLQNVKFIQEKKLIGRYFDE 184
T LSQS++FDPRLQAKI+ +VDVSYGGENGFNQAIEL+AE L NVKFIQEKKLIG+YF+E
Sbjct: 234 TELSQSELFDPRLQAKILNVVDVSYGGENGFNQAIELSAEILSNVKFIQEKKLIGKYFEE 293
Query: 185 ISQDTGKYCFGVDDTLRALELGS 253
ISQDTGKY FGV+DTL+ALE+G+
Sbjct: 294 ISQDTGKYVFGVEDTLKALEMGA 316
Score = 93.1 bits (221), Expect = 1e-19
Identities = 45/85 (52%), Positives = 57/85 (67%), Gaps = 1/85 (1%)
Frame = +1
Query: 256 ETLICWENLDIQRYVLKSHATNQETILHLTPEQEKDKSHFTDKESGVELELVECQPLLEW 435
ETLI WENLDI RY LK++ T + I HL +QE ++S+F D E+ ELE+ E PLLEW
Sbjct: 318 ETLIVWENLDINRYELKNNTTGEIVIKHLGKDQENNQSNFHDAETNAELEVQEKMPLLEW 377
Query: 436 LANNYKSFGATLESSRTRAR-GKQF 507
AN YK FG TLE +++ G QF
Sbjct: 378 FANEYKRFGCTLEFVTNKSQEGSQF 402
>At1g12920.1 68414.m01500 eukaryotic release factor 1 family protein
/ eRF1 family protein contains Pfam profiles: PF03463
eRF1 domain 1, PF03464 eRF1 domain 2, PF03465 eRF1
domain 3
Length = 434
Score = 146 bits (354), Expect = 1e-35
Identities = 67/83 (80%), Positives = 78/83 (93%)
Frame = +2
Query: 5 TRLSQSDMFDPRLQAKIIKLVDVSYGGENGFNQAIELAAESLQNVKFIQEKKLIGRYFDE 184
T LSQS++FDPRLQAKI+ +VDVSYGGENGFNQAIEL+AE L NVKFIQEKKLIG+YF+E
Sbjct: 233 TELSQSELFDPRLQAKILNVVDVSYGGENGFNQAIELSAEILSNVKFIQEKKLIGKYFEE 292
Query: 185 ISQDTGKYCFGVDDTLRALELGS 253
ISQDTGKY FGV+DTL+ALE+G+
Sbjct: 293 ISQDTGKYVFGVEDTLKALEMGA 315
Score = 92.3 bits (219), Expect = 3e-19
Identities = 44/85 (51%), Positives = 54/85 (63%), Gaps = 1/85 (1%)
Frame = +1
Query: 256 ETLICWENLDIQRYVLKSHATNQETILHLTPEQEKDKSHFTDKESGVELELVECQPLLEW 435
ETLI WENLDI RY LK+ T + + H +QE D S+F D E+ ELE+ E PLLEW
Sbjct: 317 ETLIVWENLDINRYELKNSTTGEMVVKHFGKDQESDTSNFHDSETNAELEVQEKMPLLEW 376
Query: 436 LANNYKSFGATLESSRTRAR-GKQF 507
AN YK FG TLE +++ G QF
Sbjct: 377 FANEYKRFGCTLEFVTNKSQEGSQF 401
>At5g47880.1 68418.m05915 eukaryotic peptide chain release factor
subunit 1-1 (ERF1-1) identical to SP|Q39097 Eukaryotic
peptide chain release factor subunit 1-1 (eRF1-1)
(Eukaryotic release factor 1-1) (Omnipotent suppressor
protein 1 homolog 1) (SUP1 homolog 1) {Arabidopsis
thaliana}, eukaryotic release factor 1 homolog
GI:1155261 from [Arabidopsis thaliana]; contains Pfam
profiles: PF03463 eRF1 domain 1, PF03464 eRF1 domain 2,
PF03465 eRF1 domain 3
Length = 436
Score = 142 bits (345), Expect = 1e-34
Identities = 67/83 (80%), Positives = 75/83 (90%)
Frame = +2
Query: 5 TRLSQSDMFDPRLQAKIIKLVDVSYGGENGFNQAIELAAESLQNVKFIQEKKLIGRYFDE 184
T LSQSDMFDPRL AKI+ +VDVSYGGENGFNQAIEL+AE L NVKFIQEK+LIG+YF+E
Sbjct: 235 TELSQSDMFDPRLAAKILNVVDVSYGGENGFNQAIELSAEILANVKFIQEKRLIGKYFEE 294
Query: 185 ISQDTGKYCFGVDDTLRALELGS 253
ISQDTGKY FGV+DTL ALE G+
Sbjct: 295 ISQDTGKYVFGVEDTLNALESGA 317
Score = 83.0 bits (196), Expect = 2e-16
Identities = 41/85 (48%), Positives = 54/85 (63%), Gaps = 1/85 (1%)
Frame = +1
Query: 256 ETLICWENLDIQRYVLKSHATNQETILHLTPEQEKDKSHFTDKESGVELELVECQPLLEW 435
ETLI WENLDI RYV+K+ AT + I HL EQE + +F +S + L++ E LLEW
Sbjct: 319 ETLIVWENLDINRYVMKNSATGETVIKHLNKEQEANTENFKVADSDLALDVEEKLSLLEW 378
Query: 436 LANNYKSFGATLESSRTRAR-GKQF 507
LAN Y+ FG LE +++ G QF
Sbjct: 379 LANEYRRFGCALEFVTNKSQEGSQF 403
>At5g19310.1 68418.m02301 homeotic gene regulator, putative similar to
SP|P25439 Homeotic gene regulator (Brahma protein)
{Drosophila melanogaster}; contains Pfam profiles
PF00271: Helicase conserved C-terminal domain, PF00176:
SNF2 family N-terminal domain
Length = 1064
Score = 32.7 bits (71), Expect = 0.21
Identities = 18/65 (27%), Positives = 32/65 (49%)
Frame = +1
Query: 307 SHATNQETILHLTPEQEKDKSHFTDKESGVELELVECQPLLEWLANNYKSFGATLESSRT 486
S+ + E +L + E+++++ +ESG E E +PL W N K + +S
Sbjct: 991 SNGSKAEAVLSESDEEKEEEEEERKEESGKESEEENEKPLHSWKTNKKKRSRYPVMTSSP 1050
Query: 487 RARGK 501
+RGK
Sbjct: 1051 NSRGK 1055
>At3g19470.1 68416.m02468 F-box family protein contains F-box domain
Pfam:PF00646
Length = 382
Score = 29.1 bits (62), Expect = 2.6
Identities = 15/42 (35%), Positives = 24/42 (57%), Gaps = 2/42 (4%)
Frame = +2
Query: 143 FIQEKKLIGRYFDEISQDTGK--YCFGVDDTLRALELGSARR 262
FI E+K + FD+ + Y FGVD + +A++LG + R
Sbjct: 324 FIDEEKKVAVVFDKDYDNKRNIAYIFGVDGSFKAVDLGDSDR 365
>At3g49350.1 68416.m05395 RabGAP/TBC domain-containing protein
similar to GTPase activating protein [Yarrowia
lipolytica] GI:2370595; contains Pfam profile PF00566:
TBC domain
Length = 554
Score = 28.7 bits (61), Expect = 3.5
Identities = 17/50 (34%), Positives = 23/50 (46%), Gaps = 3/50 (6%)
Frame = +1
Query: 37 ASPSQD---HKTGGRIIWRRKRFQSGYRAGCGILAECQVYTGEETNWTLL 177
A P+ D + ++ RRKR Y + IL ECQ G+ W LL
Sbjct: 486 APPTDDLVLYAIAASVLQRRKRIIERYNSMDEILRECQSMAGQLDVWKLL 535
>At4g26610.1 68417.m03835 protein kinase, putative similar to
protein kinase G11A [Oryza sativa] SWISS-PROT:P47997
Length = 506
Score = 28.3 bits (60), Expect = 4.6
Identities = 13/29 (44%), Positives = 16/29 (55%)
Frame = +1
Query: 34 SASPSQDHKTGGRIIWRRKRFQSGYRAGC 120
S +PS D KTGG + + K QSG C
Sbjct: 46 SFAPSSDSKTGGEVKFNEKSDQSGKSNTC 74
>At2g23740.1 68415.m02834 zinc finger (C2H2 type) family protein
contains Pfam profile: PF00096 zinc finger, C2H2 type
Length = 907
Score = 28.3 bits (60), Expect = 4.6
Identities = 20/64 (31%), Positives = 32/64 (50%), Gaps = 4/64 (6%)
Frame = +1
Query: 331 ILH-LTPEQEKDKSHFT---DKESGVELELVECQPLLEWLANNYKSFGATLESSRTRARG 498
+LH L P QE D+ + S +ELE+ EC ++E + + FG T + + G
Sbjct: 799 LLHPLIPRQENDRFGIAVDAGQHSNIELEVDECHCIMEAHHFSKRPFGNTAVLCKDISFG 858
Query: 499 KQFV 510
K+ V
Sbjct: 859 KESV 862
>At5g13300.1 68418.m01528 ARF GTPase-activating domain-containing
protein similar to GCN4-complementing protein (GCP1)
GI:6465806 from [Arabidopsis thaliana]
Length = 768
Score = 27.9 bits (59), Expect = 6.1
Identities = 19/50 (38%), Positives = 25/50 (50%), Gaps = 1/50 (2%)
Frame = +3
Query: 126 PCRMSSLYRRRN*L-DATLMRSHRTQESIASVSMIHCALSNSDPRDVDLL 272
P R L R RN L D T S+ + E S++HCA +D V+LL
Sbjct: 640 PKREDVLLRLRNELLDRTGSSSNISPEGSGGSSLLHCACEKADLGMVELL 689
>At4g29010.1 68417.m04147 abnormal inflorescence meristem 1 / fatty
acid multifunctional protein (AIM1) identical to
gi:4337025; contains Pfam profiles PF02737
(3-hydroxyacyl-CoA dehydrogenase, NAD binding domain),
PF00378 (enoyl-CoA hydratase/isomerase family protein),
PF00725 (3-hydroxyacyl-CoA dehydrogenase)
Length = 721
Score = 27.5 bits (58), Expect = 8.1
Identities = 16/40 (40%), Positives = 23/40 (57%), Gaps = 1/40 (2%)
Frame = +3
Query: 201 ESIASVSMIHCAL-SNSDPRDVDLLGEPRHTKIRIEVACH 317
+ I V HC L SN+ D+D++GE ++K RI V H
Sbjct: 408 KEIEKVCSPHCILASNTSTIDLDVIGEKTNSKDRI-VGAH 446
>At3g56700.1 68416.m06307 male sterility protein, putative similar
to SP|Q08891 Male sterility protein 2 {Arabidopsis
thaliana}; contains Pfam profile PF03015: Male sterility
protein
Length = 527
Score = 27.5 bits (58), Expect = 8.1
Identities = 10/22 (45%), Positives = 15/22 (68%)
Frame = -1
Query: 370 VICPSLVQASGGELFPGWWHAT 305
+I PS++++S E FPGW T
Sbjct: 301 IIRPSIIESSYNEPFPGWIQGT 322
>At3g16940.1 68416.m02165 calmodulin-binding protein similar to
anther ethylene-upregulated protein ER1 GI:11612392 from
[Nicotiana tabacum]; contains Pfam profile: PF00612 IQ
calmodulin-binding motif (3 copies)
Length = 852
Score = 27.5 bits (58), Expect = 8.1
Identities = 15/37 (40%), Positives = 19/37 (51%)
Frame = +1
Query: 493 RGKQFVAASRDGGSSVQSDFHRCSSRQEIDNLTTSTL 603
RG Q VAA D Q DF++ S RQ + L S +
Sbjct: 775 RGLQ-VAAEEDSPGEAQEDFYKTSQRQAEERLERSVV 810
>At2g41520.2 68415.m05131 DNAJ heat shock N-terminal domain-containing
protein contains Pfam profiles PF00226: DnaJ domain,
PF00515: TPR Domain
Length = 1077
Score = 27.5 bits (58), Expect = 8.1
Identities = 10/24 (41%), Positives = 12/24 (50%)
Frame = -2
Query: 87 SPPYDTSTSFMILAWRRGSNISDW 16
SPPY TS + +WR N W
Sbjct: 1053 SPPYQTSRRYWKDSWRTNQNTPSW 1076
>At2g41520.1 68415.m05130 DNAJ heat shock N-terminal domain-containing
protein contains Pfam profiles PF00226: DnaJ domain,
PF00515: TPR Domain
Length = 1108
Score = 27.5 bits (58), Expect = 8.1
Identities = 10/24 (41%), Positives = 12/24 (50%)
Frame = -2
Query: 87 SPPYDTSTSFMILAWRRGSNISDW 16
SPPY TS + +WR N W
Sbjct: 1084 SPPYQTSRRYWKDSWRTNQNTPSW 1107
>At1g49990.1 68414.m05610 expressed protein ; expression supported
by MPSS
Length = 371
Score = 27.5 bits (58), Expect = 8.1
Identities = 11/28 (39%), Positives = 16/28 (57%)
Frame = -3
Query: 569 RELHRWKSLCTEEPPSLEAATNCFPLAL 486
+E RW+ + PS + NCFPLA+
Sbjct: 261 KESERWQLIWEVVMPSFISDVNCFPLAM 288
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 15,035,226
Number of Sequences: 28952
Number of extensions: 317309
Number of successful extensions: 943
Number of sequences better than 10.0: 15
Number of HSP's better than 10.0 without gapping: 915
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 943
length of database: 12,070,560
effective HSP length: 78
effective length of database: 9,812,304
effective search space used: 1334473344
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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