BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= NV060474.seq (686 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At5g19820.1 68418.m02355 PBS lyase HEAT-like repeat-containing p... 58 5e-09 At5g24310.2 68418.m02860 expressed protein strong similarity to ... 32 0.31 At5g24310.1 68418.m02861 expressed protein strong similarity to ... 32 0.31 At2g38080.1 68415.m04674 laccase, putative / diphenol oxidase, p... 29 2.2 At2g16950.1 68415.m01953 importin beta-2 subunit family protein ... 29 2.9 At5g43530.1 68418.m05322 SNF2 domain-containing protein / helica... 28 6.7 At1g33320.1 68414.m04121 cystathionine gamma-synthase, chloropla... 28 6.7 At1g10340.2 68414.m01165 ankyrin repeat family protein contains ... 28 6.7 >At5g19820.1 68418.m02355 PBS lyase HEAT-like repeat-containing protein contains Pfam profile: PF03130 PBS lyase HEAT-like repeat Length = 1116 Score = 58.0 bits (134), Expect = 5e-09 Identities = 27/71 (38%), Positives = 40/71 (56%) Frame = +2 Query: 272 NYVAAESALDRMCCGLGGKIMLGLIVGQVPVMLQSEDWXXRHAALMAVSSAGEGCHKQME 451 NY + LDR+ LGG ++ + Q L + +W HA+L+A++ EGC K M Sbjct: 355 NYSMGQECLDRLAISLGGNTIVPVAYQQFSAYLAASEWQKHHASLIALAQIAEGCSKVMI 414 Query: 452 QMLDQVVTAVL 484 + LDQVV+ VL Sbjct: 415 KNLDQVVSMVL 425 Score = 48.4 bits (110), Expect = 4e-06 Identities = 26/77 (33%), Positives = 46/77 (59%), Gaps = 3/77 (3%) Frame = +3 Query: 12 KFLRPQLETIFQVCIKVVGDKDAEDNWRQLALEAMVTLCET---APAMVRKQVPNAVSML 182 +FLR QL I +++ E++ R LA+E +VTL E AP MVRK +P + L Sbjct: 265 RFLRRQLVDIVGSMLQIAEADSLEESTRHLAIEFLVTLAEARERAPGMVRK-LPQFIDRL 323 Query: 183 TPLVLEMMCELDEEPDW 233 ++++M+ +++++P W Sbjct: 324 FAVLMKMLEDIEDDPAW 340 >At5g24310.2 68418.m02860 expressed protein strong similarity to unknown protein (emb|CAB66408.1) Length = 240 Score = 32.3 bits (70), Expect = 0.31 Identities = 16/43 (37%), Positives = 20/43 (46%) Frame = +3 Query: 60 VVGDKDAEDNWRQLALEAMVTLCETAPAMVRKQVPNAVSMLTP 188 V G D ED+W Q T+ ET P VRK + + S P Sbjct: 159 VEGSFDGEDDWNQFRNAVRATIRETPPPPVRKPILQSPSQRKP 201 >At5g24310.1 68418.m02861 expressed protein strong similarity to unknown protein (emb|CAB66408.1) Length = 321 Score = 32.3 bits (70), Expect = 0.31 Identities = 16/43 (37%), Positives = 20/43 (46%) Frame = +3 Query: 60 VVGDKDAEDNWRQLALEAMVTLCETAPAMVRKQVPNAVSMLTP 188 V G D ED+W Q T+ ET P VRK + + S P Sbjct: 159 VEGSFDGEDDWNQFRNAVRATIRETPPPPVRKPILQSPSQRKP 201 >At2g38080.1 68415.m04674 laccase, putative / diphenol oxidase, putative similar to diphenol oxidase [Nicotiana tabacum][GI:1685087] Length = 558 Score = 29.5 bits (63), Expect = 2.2 Identities = 15/45 (33%), Positives = 23/45 (51%) Frame = -1 Query: 272 SGHYRHSSVVLYGPVRLFIKLTHHLKYKWSEHAHGIGHLFTHHGW 138 +G Y ++ L IK+ +H+KY S H HG+ + T GW Sbjct: 51 NGRYPGPTIYAREDDTLLIKVVNHVKYNVSIHWHGVRQVRT--GW 93 >At2g16950.1 68415.m01953 importin beta-2 subunit family protein similar to SP|Q92973 Importin beta-2 subunit (Transportin) {Homo sapiens}; contains Pfam profile PF03810: Importin-beta N-terminal domain Length = 891 Score = 29.1 bits (62), Expect = 2.9 Identities = 12/36 (33%), Positives = 20/36 (55%) Frame = +2 Query: 377 EDWXXRHAALMAVSSAGEGCHKQMEQMLDQVVTAVL 484 E W R AA++A+ + EGC + L ++V +L Sbjct: 405 EAWKQREAAVLALGAIAEGCMNGLYPHLSEIVAFLL 440 >At5g43530.1 68418.m05322 SNF2 domain-containing protein / helicase domain-containing protein / RING finger domain-containing protein similar to SP|P36607 DNA repair protein rad8 {Schizosaccharomyces pombe}; contains Pfam profiles PF00271: Helicase conserved C-terminal domain, PF00176: SNF2 family N-terminal domain, PF00097: Zinc finger, C3HC4 type (RING finger) Length = 1277 Score = 27.9 bits (59), Expect = 6.7 Identities = 12/33 (36%), Positives = 19/33 (57%) Frame = +2 Query: 368 LQSEDWXXRHAALMAVSSAGEGCHKQMEQMLDQ 466 L ED AAL+A++ +GC + +EQ D+ Sbjct: 551 LNLEDDYDERAALLAIAKRRKGCQQSLEQNKDE 583 >At1g33320.1 68414.m04121 cystathionine gamma-synthase, chloroplast, putative / O-succinylhomoserine (Thiol)-lyase, putative strong similarity to SP|P55217 Cystathionine gamma-synthase, chloroplast precursor (EC 4.2.99.9) (CGS) (O-succinylhomoserine (Thiol)-lyase) {Arabidopsis thaliana}; contains Pfam profile PF01053: Cys/Met metabolism PLP-dependent enzyme Length = 412 Score = 27.9 bits (59), Expect = 6.7 Identities = 9/21 (42%), Positives = 15/21 (71%) Frame = +2 Query: 263 NDQNYVAAESALDRMCCGLGG 325 ++Q +V ES ++R+CCG G Sbjct: 9 SNQRFVTRESEVNRICCGFNG 29 >At1g10340.2 68414.m01165 ankyrin repeat family protein contains ankyrin repeat domains, Pfam:PF00023 Length = 574 Score = 27.9 bits (59), Expect = 6.7 Identities = 13/58 (22%), Positives = 29/58 (50%) Frame = +3 Query: 93 RQLALEAMVTLCETAPAMVRKQVPNAVSMLTPLVLEMMCELDEEPDWTIQDNTAMTIM 266 R ++EA + E ++ ++ A+S + +LE +L E W ++D + T++ Sbjct: 114 RSNSIEAARLIAEKTQSIGLGELILAISSIVGTILERFPDLAREEAWVVEDGSQSTLL 171 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 15,110,287 Number of Sequences: 28952 Number of extensions: 324028 Number of successful extensions: 784 Number of sequences better than 10.0: 8 Number of HSP's better than 10.0 without gapping: 760 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 784 length of database: 12,070,560 effective HSP length: 79 effective length of database: 9,783,352 effective search space used: 1457719448 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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