BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= NV060473.seq (428 letters) Database: nematostella 59,808 sequences; 16,821,457 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SB_37381| Best HMM Match : Ribosomal_S12 (HMM E-Value=0) 125 1e-29 SB_33031| Best HMM Match : Ribosomal_S12 (HMM E-Value=0) 65 2e-11 SB_34124| Best HMM Match : No HMM Matches (HMM E-Value=.) 29 1.6 SB_19884| Best HMM Match : No HMM Matches (HMM E-Value=.) 28 2.9 SB_46453| Best HMM Match : DUF947 (HMM E-Value=0.13) 28 2.9 SB_32242| Best HMM Match : Pam16 (HMM E-Value=7.4e-20) 27 6.6 SB_37206| Best HMM Match : MFS_1 (HMM E-Value=3.4e-17) 27 6.6 SB_20006| Best HMM Match : No HMM Matches (HMM E-Value=.) 27 8.7 >SB_37381| Best HMM Match : Ribosomal_S12 (HMM E-Value=0) Length = 413 Score = 125 bits (302), Expect = 1e-29 Identities = 56/66 (84%), Positives = 62/66 (93%) Frame = +3 Query: 63 NHRREQRWADKEFKKAHMGTKWKANPFGGASHAKGIVLEKVGVEAKQPNSAIRKCVRVQL 242 +HRR+Q+W DK +KKAH+GT KANPFGGASHAKGIVLEKVGVEAKQPNSAIRKCVRVQL Sbjct: 14 SHRRDQKWHDKAYKKAHLGTALKANPFGGASHAKGIVLEKVGVEAKQPNSAIRKCVRVQL 73 Query: 243 IKNGRK 260 IKNG+K Sbjct: 74 IKNGKK 79 Score = 45.6 bits (103), Expect = 2e-05 Identities = 18/21 (85%), Positives = 20/21 (95%) Frame = +2 Query: 254 KKVTAFVPRDGCLNHIEENDE 316 KK+TAFVP DGCLN+IEENDE Sbjct: 78 KKITAFVPNDGCLNYIEENDE 98 Score = 36.3 bits (80), Expect = 0.011 Identities = 13/17 (76%), Positives = 16/17 (94%) Frame = +1 Query: 322 VAGFGRXGHAVGDIPGV 372 ++GFGR GHAVGDIPG+ Sbjct: 101 ISGFGRRGHAVGDIPGI 117 >SB_33031| Best HMM Match : Ribosomal_S12 (HMM E-Value=0) Length = 143 Score = 65.3 bits (152), Expect = 2e-11 Identities = 29/35 (82%), Positives = 33/35 (94%) Frame = +1 Query: 322 VAGFGRXGHAVGDIPGVRYXVVKVANVSLLALYKE 426 ++GFGR GHAVGDIPGVR+ VVKVANVSLLAL+KE Sbjct: 102 ISGFGRRGHAVGDIPGVRFKVVKVANVSLLALFKE 136 Score = 54.0 bits (124), Expect = 5e-08 Identities = 24/28 (85%), Positives = 27/28 (96%) Frame = +3 Query: 177 EKVGVEAKQPNSAIRKCVRVQLIKNGRK 260 ++ GVEAKQPNSAIRKCVRVQLIKNG+K Sbjct: 53 QEPGVEAKQPNSAIRKCVRVQLIKNGKK 80 Score = 45.6 bits (103), Expect = 2e-05 Identities = 18/21 (85%), Positives = 20/21 (95%) Frame = +2 Query: 254 KKVTAFVPRDGCLNHIEENDE 316 KK+TAFVP DGCLN+IEENDE Sbjct: 79 KKITAFVPNDGCLNYIEENDE 99 >SB_34124| Best HMM Match : No HMM Matches (HMM E-Value=.) Length = 468 Score = 29.1 bits (62), Expect = 1.6 Identities = 15/33 (45%), Positives = 18/33 (54%) Frame = +2 Query: 107 SPHGYEMEG*PFRWCISRKGHRPRESWCRS*AA 205 S HG MEG P W +S G P+ S C S +A Sbjct: 85 SQHGTRMEGVPVCWYVSVWGLSPQVSQCDSVSA 117 >SB_19884| Best HMM Match : No HMM Matches (HMM E-Value=.) Length = 3486 Score = 28.3 bits (60), Expect = 2.9 Identities = 17/60 (28%), Positives = 32/60 (53%), Gaps = 3/60 (5%) Frame = -1 Query: 287 NRHGGRMRSLS--SVLNELYTDAFADGRVGLLSFYTNFLEDDALCVRCTTE-RVSLPFRT 117 +R+GGRM+SL+ S N + D + + + + ED++ + +T + LP+RT Sbjct: 1851 SRNGGRMKSLANRSGRNGVIRDLLGSQALDTAASFESLTEDESAAITGSTVCSIPLPYRT 1910 >SB_46453| Best HMM Match : DUF947 (HMM E-Value=0.13) Length = 943 Score = 28.3 bits (60), Expect = 2.9 Identities = 17/35 (48%), Positives = 22/35 (62%), Gaps = 3/35 (8%) Frame = -2 Query: 175 RTMPFA*D-APPKGLAFHFVPMW-AFLN-SLSAHR 80 R +PF+ APPKG F VP+W +F N S+ HR Sbjct: 902 RKVPFSSVLAPPKGYRFLIVPLWRSFSNRSVFGHR 936 >SB_32242| Best HMM Match : Pam16 (HMM E-Value=7.4e-20) Length = 255 Score = 27.1 bits (57), Expect = 6.6 Identities = 10/24 (41%), Positives = 18/24 (75%) Frame = +1 Query: 88 RTKNSRKPTWVRNGRLTLSVVHLT 159 R N++KP +R+GR+T+S+ +T Sbjct: 110 RDTNTKKPVHLRDGRVTVSLAAMT 133 >SB_37206| Best HMM Match : MFS_1 (HMM E-Value=3.4e-17) Length = 1088 Score = 27.1 bits (57), Expect = 6.6 Identities = 13/31 (41%), Positives = 16/31 (51%) Frame = -2 Query: 268 CGHFLPFLMSCTRTHLRMAELGCLASTPTFS 176 CG FL F M+ + ELGC+ S P S Sbjct: 608 CGAFLTFGMAAAVIGPTLLELGCVTSRPVNS 638 >SB_20006| Best HMM Match : No HMM Matches (HMM E-Value=.) Length = 608 Score = 26.6 bits (56), Expect = 8.7 Identities = 16/37 (43%), Positives = 19/37 (51%) Frame = -1 Query: 341 LRPNPATSIRRFLRCGLGNRHGGRMRSLSSVLNELYT 231 LR +I R L+ GL N LSSV N+LYT Sbjct: 504 LRKEALENICRSLKAGLTNDENCVQAFLSSVSNKLYT 540 Database: nematostella Posted date: Oct 22, 2007 1:22 PM Number of letters in database: 16,821,457 Number of sequences in database: 59,808 Lambda K H 0.316 0.132 0.379 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 14,142,662 Number of Sequences: 59808 Number of extensions: 305951 Number of successful extensions: 584 Number of sequences better than 10.0: 8 Number of HSP's better than 10.0 without gapping: 540 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 584 length of database: 16,821,457 effective HSP length: 75 effective length of database: 12,335,857 effective search space used: 826502419 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.6 bits)
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