BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= NV060465.seq
(662 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPCC338.11c |rrg1|uvi22|methyltransferase |Schizosaccharomyces p... 34 0.016
SPAC3A11.03 |||methyltransferase |Schizosaccharomyces pombe|chr ... 33 0.037
SPAC8F11.09c |nnt1||nicotinamide N-methyltransferase Nnt1 |Schiz... 33 0.049
SPAC23H4.01c ||SPAP27G11.01|sterol binding ankyrin repeat protei... 30 0.26
SPBC365.15 |alp4||gamma tubulin complex Spc97/GCP2 subunit Alp4|... 30 0.34
SPBP16F5.03c |||phosphatidylinositol kinase |Schizosaccharomyces... 27 3.2
SPAC1071.11 |||NADH-dependent flavin oxidoreductase |Schizosacch... 27 3.2
SPAC1B3.15c |||membrane transporter|Schizosaccharomyces pombe|ch... 27 3.2
SPBC1105.17 |cnp1|sim2|CENP-A|Schizosaccharomyces pombe|chr 2|||... 27 3.2
SPBC8D2.10c |rmt3|rmt3|type I ribosomal protein arginine N-methy... 26 4.2
SPBC3H7.09 |mug142||palmitoyltransferase|Schizosaccharomyces pom... 25 7.4
>SPCC338.11c |rrg1|uvi22|methyltransferase |Schizosaccharomyces
pombe|chr 3|||Manual
Length = 303
Score = 34.3 bits (75), Expect = 0.016
Identities = 19/38 (50%), Positives = 20/38 (52%)
Frame = +1
Query: 139 FYTWPSAPLLXWYLWTQRRHIRGLRVLELGCGTGLPGI 252
F TW SAPLL L + LELG GTGL GI
Sbjct: 114 FKTWGSAPLLSANLPKWEDLSNSINALELGAGTGLVGI 151
>SPAC3A11.03 |||methyltransferase |Schizosaccharomyces pombe|chr
1|||Manual
Length = 247
Score = 33.1 bits (72), Expect = 0.037
Identities = 18/37 (48%), Positives = 22/37 (59%)
Frame = +1
Query: 145 TWPSAPLLXWYLWTQRRHIRGLRVLELGCGTGLPGIL 255
TW + L Y++ Q G+RVLELG GTGL IL
Sbjct: 152 TWEAGMALAEYIY-QHPVQSGMRVLELGAGTGLVSIL 187
>SPAC8F11.09c |nnt1||nicotinamide N-methyltransferase Nnt1
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 255
Score = 32.7 bits (71), Expect = 0.049
Identities = 16/41 (39%), Positives = 22/41 (53%)
Frame = +1
Query: 133 YSFYTWPSAPLLXWYLWTQRRHIRGLRVLELGCGTGLPGIL 255
++ Y W S L Y+ +R +VLELG G GLP I+
Sbjct: 53 WAHYLWNSGIELANYIDKNPDTVRAKKVLELGAGAGLPSIV 93
>SPAC23H4.01c ||SPAP27G11.01|sterol binding ankyrin repeat
protein|Schizosaccharomyces pombe|chr 1|||Manual
Length = 945
Score = 30.3 bits (65), Expect = 0.26
Identities = 18/51 (35%), Positives = 25/51 (49%)
Frame = -3
Query: 567 SIDQSVLRSW*EHKKRAXSASSRYATVDSTSSNCDGS*KQSDARIRSTGRK 415
+++ + L SW H KR Y + TSS DGS K +D I T R+
Sbjct: 17 TVESNSLLSWQLHVKRKSIKFDIYHKKNDTSSLLDGSNKNTDRSILHTKRQ 67
>SPBC365.15 |alp4||gamma tubulin complex Spc97/GCP2 subunit
Alp4|Schizosaccharomyces pombe|chr 2|||Manual
Length = 784
Score = 29.9 bits (64), Expect = 0.34
Identities = 13/47 (27%), Positives = 26/47 (55%)
Frame = +2
Query: 368 QIVGLTWGLFLGDLHNLRPVDLILASDCFYEPSQFEEVLSTVAYLLE 508
+++ WG F+G+L N R VD ++ + + +E + T + LL+
Sbjct: 655 EVIETHWGKFMGELENARTVDNLMQEHIDFLDTCLKECMLTNSRLLK 701
>SPBP16F5.03c |||phosphatidylinositol kinase |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 3699
Score = 26.6 bits (56), Expect = 3.2
Identities = 14/27 (51%), Positives = 20/27 (74%), Gaps = 1/27 (3%)
Frame = -2
Query: 631 SLSEVPGPRKCTFESPL-LQ*SFNRPV 554
SLS+VP PRK TF+ + LQ S ++P+
Sbjct: 2708 SLSDVPTPRKLTFQCFMTLQKSVSQPL 2734
>SPAC1071.11 |||NADH-dependent flavin oxidoreductase
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 244
Score = 26.6 bits (56), Expect = 3.2
Identities = 15/35 (42%), Positives = 21/35 (60%), Gaps = 1/35 (2%)
Frame = +2
Query: 50 FSAQDPLQTATLE-VNIWRLLSRSYCQPVIRFTRG 151
FS + TA+ + VN +RLL R + QPV+ T G
Sbjct: 25 FSNFNRYSTASHDNVNAFRLLMRRFAQPVVIITSG 59
>SPAC1B3.15c |||membrane transporter|Schizosaccharomyces pombe|chr
1|||Manual
Length = 628
Score = 26.6 bits (56), Expect = 3.2
Identities = 13/47 (27%), Positives = 23/47 (48%)
Frame = -3
Query: 393 KPHVRPTICKSRLGTKPLASQQAER*RSDLGNVRLSVRVTSLRTSRQ 253
KP + P+I S KP +SQ + +L ++ +S+ S+Q
Sbjct: 9 KPSISPSIASSFPTVKPFSSQNSTTSNPELSHIEKESNASSIIISQQ 55
>SPBC1105.17 |cnp1|sim2|CENP-A|Schizosaccharomyces pombe|chr
2|||Manual
Length = 120
Score = 26.6 bits (56), Expect = 3.2
Identities = 12/29 (41%), Positives = 15/29 (51%), Gaps = 1/29 (3%)
Frame = -1
Query: 248 PGSPVPQPNSNTLRPRICRLC-VHKYQXS 165
PG P+P+P RP L + KYQ S
Sbjct: 10 PGDPIPRPRKKRYRPGTTALREIRKYQRS 38
>SPBC8D2.10c |rmt3|rmt3|type I ribosomal protein arginine
N-methytransferase Rmt3|Schizosaccharomyces pombe|chr
2|||Manual
Length = 543
Score = 26.2 bits (55), Expect = 4.2
Identities = 13/36 (36%), Positives = 20/36 (55%), Gaps = 1/36 (2%)
Frame = +1
Query: 193 RHI-RGLRVLELGCGTGLPGILPRSAEARNPHRQSN 297
+HI G VL++GCGTG+ + A A+ + N
Sbjct: 251 KHIFAGKTVLDVGCGTGILSMFCAKAGAKKVYAVDN 286
>SPBC3H7.09 |mug142||palmitoyltransferase|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 350
Score = 25.4 bits (53), Expect = 7.4
Identities = 20/61 (32%), Positives = 32/61 (52%), Gaps = 6/61 (9%)
Frame = +1
Query: 103 IVIPELLSAGYS-FYTW----PSAPLLXWYLWTQRRHIRGLRVLEL-GCGTGLPGILPRS 264
+++P +L +S F+ W P+ P+ YL+ L V+ + C T PGILPR+
Sbjct: 94 LILPGVLFFIFSAFWLWHHVSPAVPITFAYLYA-------LAVVSMFKCSTADPGILPRN 146
Query: 265 A 267
A
Sbjct: 147 A 147
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,703,837
Number of Sequences: 5004
Number of extensions: 54165
Number of successful extensions: 153
Number of sequences better than 10.0: 11
Number of HSP's better than 10.0 without gapping: 146
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 152
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 301829700
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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