BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= NV060465.seq (662 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPCC338.11c |rrg1|uvi22|methyltransferase |Schizosaccharomyces p... 34 0.016 SPAC3A11.03 |||methyltransferase |Schizosaccharomyces pombe|chr ... 33 0.037 SPAC8F11.09c |nnt1||nicotinamide N-methyltransferase Nnt1 |Schiz... 33 0.049 SPAC23H4.01c ||SPAP27G11.01|sterol binding ankyrin repeat protei... 30 0.26 SPBC365.15 |alp4||gamma tubulin complex Spc97/GCP2 subunit Alp4|... 30 0.34 SPBP16F5.03c |||phosphatidylinositol kinase |Schizosaccharomyces... 27 3.2 SPAC1071.11 |||NADH-dependent flavin oxidoreductase |Schizosacch... 27 3.2 SPAC1B3.15c |||membrane transporter|Schizosaccharomyces pombe|ch... 27 3.2 SPBC1105.17 |cnp1|sim2|CENP-A|Schizosaccharomyces pombe|chr 2|||... 27 3.2 SPBC8D2.10c |rmt3|rmt3|type I ribosomal protein arginine N-methy... 26 4.2 SPBC3H7.09 |mug142||palmitoyltransferase|Schizosaccharomyces pom... 25 7.4 >SPCC338.11c |rrg1|uvi22|methyltransferase |Schizosaccharomyces pombe|chr 3|||Manual Length = 303 Score = 34.3 bits (75), Expect = 0.016 Identities = 19/38 (50%), Positives = 20/38 (52%) Frame = +1 Query: 139 FYTWPSAPLLXWYLWTQRRHIRGLRVLELGCGTGLPGI 252 F TW SAPLL L + LELG GTGL GI Sbjct: 114 FKTWGSAPLLSANLPKWEDLSNSINALELGAGTGLVGI 151 >SPAC3A11.03 |||methyltransferase |Schizosaccharomyces pombe|chr 1|||Manual Length = 247 Score = 33.1 bits (72), Expect = 0.037 Identities = 18/37 (48%), Positives = 22/37 (59%) Frame = +1 Query: 145 TWPSAPLLXWYLWTQRRHIRGLRVLELGCGTGLPGIL 255 TW + L Y++ Q G+RVLELG GTGL IL Sbjct: 152 TWEAGMALAEYIY-QHPVQSGMRVLELGAGTGLVSIL 187 >SPAC8F11.09c |nnt1||nicotinamide N-methyltransferase Nnt1 |Schizosaccharomyces pombe|chr 1|||Manual Length = 255 Score = 32.7 bits (71), Expect = 0.049 Identities = 16/41 (39%), Positives = 22/41 (53%) Frame = +1 Query: 133 YSFYTWPSAPLLXWYLWTQRRHIRGLRVLELGCGTGLPGIL 255 ++ Y W S L Y+ +R +VLELG G GLP I+ Sbjct: 53 WAHYLWNSGIELANYIDKNPDTVRAKKVLELGAGAGLPSIV 93 >SPAC23H4.01c ||SPAP27G11.01|sterol binding ankyrin repeat protein|Schizosaccharomyces pombe|chr 1|||Manual Length = 945 Score = 30.3 bits (65), Expect = 0.26 Identities = 18/51 (35%), Positives = 25/51 (49%) Frame = -3 Query: 567 SIDQSVLRSW*EHKKRAXSASSRYATVDSTSSNCDGS*KQSDARIRSTGRK 415 +++ + L SW H KR Y + TSS DGS K +D I T R+ Sbjct: 17 TVESNSLLSWQLHVKRKSIKFDIYHKKNDTSSLLDGSNKNTDRSILHTKRQ 67 >SPBC365.15 |alp4||gamma tubulin complex Spc97/GCP2 subunit Alp4|Schizosaccharomyces pombe|chr 2|||Manual Length = 784 Score = 29.9 bits (64), Expect = 0.34 Identities = 13/47 (27%), Positives = 26/47 (55%) Frame = +2 Query: 368 QIVGLTWGLFLGDLHNLRPVDLILASDCFYEPSQFEEVLSTVAYLLE 508 +++ WG F+G+L N R VD ++ + + +E + T + LL+ Sbjct: 655 EVIETHWGKFMGELENARTVDNLMQEHIDFLDTCLKECMLTNSRLLK 701 >SPBP16F5.03c |||phosphatidylinositol kinase |Schizosaccharomyces pombe|chr 2|||Manual Length = 3699 Score = 26.6 bits (56), Expect = 3.2 Identities = 14/27 (51%), Positives = 20/27 (74%), Gaps = 1/27 (3%) Frame = -2 Query: 631 SLSEVPGPRKCTFESPL-LQ*SFNRPV 554 SLS+VP PRK TF+ + LQ S ++P+ Sbjct: 2708 SLSDVPTPRKLTFQCFMTLQKSVSQPL 2734 >SPAC1071.11 |||NADH-dependent flavin oxidoreductase |Schizosaccharomyces pombe|chr 1|||Manual Length = 244 Score = 26.6 bits (56), Expect = 3.2 Identities = 15/35 (42%), Positives = 21/35 (60%), Gaps = 1/35 (2%) Frame = +2 Query: 50 FSAQDPLQTATLE-VNIWRLLSRSYCQPVIRFTRG 151 FS + TA+ + VN +RLL R + QPV+ T G Sbjct: 25 FSNFNRYSTASHDNVNAFRLLMRRFAQPVVIITSG 59 >SPAC1B3.15c |||membrane transporter|Schizosaccharomyces pombe|chr 1|||Manual Length = 628 Score = 26.6 bits (56), Expect = 3.2 Identities = 13/47 (27%), Positives = 23/47 (48%) Frame = -3 Query: 393 KPHVRPTICKSRLGTKPLASQQAER*RSDLGNVRLSVRVTSLRTSRQ 253 KP + P+I S KP +SQ + +L ++ +S+ S+Q Sbjct: 9 KPSISPSIASSFPTVKPFSSQNSTTSNPELSHIEKESNASSIIISQQ 55 >SPBC1105.17 |cnp1|sim2|CENP-A|Schizosaccharomyces pombe|chr 2|||Manual Length = 120 Score = 26.6 bits (56), Expect = 3.2 Identities = 12/29 (41%), Positives = 15/29 (51%), Gaps = 1/29 (3%) Frame = -1 Query: 248 PGSPVPQPNSNTLRPRICRLC-VHKYQXS 165 PG P+P+P RP L + KYQ S Sbjct: 10 PGDPIPRPRKKRYRPGTTALREIRKYQRS 38 >SPBC8D2.10c |rmt3|rmt3|type I ribosomal protein arginine N-methytransferase Rmt3|Schizosaccharomyces pombe|chr 2|||Manual Length = 543 Score = 26.2 bits (55), Expect = 4.2 Identities = 13/36 (36%), Positives = 20/36 (55%), Gaps = 1/36 (2%) Frame = +1 Query: 193 RHI-RGLRVLELGCGTGLPGILPRSAEARNPHRQSN 297 +HI G VL++GCGTG+ + A A+ + N Sbjct: 251 KHIFAGKTVLDVGCGTGILSMFCAKAGAKKVYAVDN 286 >SPBC3H7.09 |mug142||palmitoyltransferase|Schizosaccharomyces pombe|chr 2|||Manual Length = 350 Score = 25.4 bits (53), Expect = 7.4 Identities = 20/61 (32%), Positives = 32/61 (52%), Gaps = 6/61 (9%) Frame = +1 Query: 103 IVIPELLSAGYS-FYTW----PSAPLLXWYLWTQRRHIRGLRVLEL-GCGTGLPGILPRS 264 +++P +L +S F+ W P+ P+ YL+ L V+ + C T PGILPR+ Sbjct: 94 LILPGVLFFIFSAFWLWHHVSPAVPITFAYLYA-------LAVVSMFKCSTADPGILPRN 146 Query: 265 A 267 A Sbjct: 147 A 147 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,703,837 Number of Sequences: 5004 Number of extensions: 54165 Number of successful extensions: 153 Number of sequences better than 10.0: 11 Number of HSP's better than 10.0 without gapping: 146 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 152 length of database: 2,362,478 effective HSP length: 70 effective length of database: 2,012,198 effective search space used: 301829700 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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