BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= NV060465.seq (662 letters) Database: bee 438 sequences; 146,343 total letters Searching......................................................done Score E Sequences producing significant alignments: (bits) Value DQ667193-1|ABG75745.1| 510|Apis mellifera cys-loop ligand-gated... 25 0.49 DQ026031-1|AAY87890.1| 601|Apis mellifera nicotinic acetylcholi... 22 6.0 AY540846-1|AAS48080.1| 541|Apis mellifera neuronal nicotinic ac... 21 7.9 AF159569-1|AAF70859.1| 1124|Apis mellifera period clock protein ... 21 7.9 >DQ667193-1|ABG75745.1| 510|Apis mellifera cys-loop ligand-gated ion channel subunit protein. Length = 510 Score = 25.4 bits (53), Expect = 0.49 Identities = 7/9 (77%), Positives = 9/9 (100%) Frame = +3 Query: 393 CFWVIYITY 419 C+WVIY+TY Sbjct: 486 CYWVIYVTY 494 Score = 21.4 bits (43), Expect = 7.9 Identities = 10/16 (62%), Positives = 12/16 (75%) Frame = +3 Query: 588 DSKVHFLGPGTSLSES 635 DS++ FLGP SLS S Sbjct: 95 DSRLSFLGPIKSLSLS 110 >DQ026031-1|AAY87890.1| 601|Apis mellifera nicotinic acetylcholine receptor alpha1subunit protein. Length = 601 Score = 21.8 bits (44), Expect = 6.0 Identities = 7/24 (29%), Positives = 14/24 (58%) Frame = -1 Query: 368 ASHDWVPSR*LHNKQKGDEAISVM 297 + H W+P L+N G+ +++M Sbjct: 99 SEHIWLPDIVLYNNADGNYEVTIM 122 >AY540846-1|AAS48080.1| 541|Apis mellifera neuronal nicotinic acetylcholine receptorApisa2 subunit protein. Length = 541 Score = 21.4 bits (43), Expect = 7.9 Identities = 7/24 (29%), Positives = 13/24 (54%) Frame = -1 Query: 368 ASHDWVPSR*LHNKQKGDEAISVM 297 + H W+P L+N G+ ++ M Sbjct: 99 SEHIWLPDIVLYNNADGEYGVTTM 122 >AF159569-1|AAF70859.1| 1124|Apis mellifera period clock protein protein. Length = 1124 Score = 21.4 bits (43), Expect = 7.9 Identities = 12/48 (25%), Positives = 23/48 (47%), Gaps = 3/48 (6%) Frame = -2 Query: 244 AVLFRNLTPTL---SDREYVAFASTSTKXAEAQMATCKTNNRLTIAPG 110 ++ N PT+ SDR+ A +TS K + +++ ++PG Sbjct: 868 SISHENNQPTMNKCSDRKRPASQATSVKAEPGSIMAMSESSKKVLSPG 915 Database: bee Posted date: Oct 23, 2007 1:17 PM Number of letters in database: 146,343 Number of sequences in database: 438 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 179,395 Number of Sequences: 438 Number of extensions: 3992 Number of successful extensions: 6 Number of sequences better than 10.0: 4 Number of HSP's better than 10.0 without gapping: 5 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 6 length of database: 146,343 effective HSP length: 56 effective length of database: 121,815 effective search space used: 19977660 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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