Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= NV060463.seq
(671 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC23A1.12c |||phenylalanine-tRNA ligase beta subunit |Schizosa... 68 1e-12
SPAC19D5.04 |ptr1||HECT domain|Schizosaccharomyces pombe|chr 1||... 36 0.007
SPAC4H3.12c |||sequence orphan|Schizosaccharomyces pombe|chr 1||... 27 2.5
SPCC162.09c |hmg1||3-hydroxy-3-methylglutaryl-CoA reductase|Schi... 27 3.3
SPCC14G10.02 ||SPCC18B5.13|ribosome biogenesis protein Urb1|Schi... 26 5.7
SPAC167.06c |mug143|SPAC57A7.02c|sequence orphan|Schizosaccharom... 25 7.5
SPBC29A3.09c |||AAA family ATPase Gcn20 |Schizosaccharomyces pom... 25 7.5
SPAC144.05 |||ATP-dependent DNA helicase|Schizosaccharomyces pom... 25 7.5
SPAC13F5.03c |||glycerol dehydrogenase |Schizosaccharomyces pomb... 25 7.5
SPCC132.04c |||NAD-dependent glutamate dehydrogenase |Schizosacc... 25 9.9
SPAPJ698.03c |prp12|sap130|U2 snRNP-associated protein Sap130 |S... 25 9.9
>SPAC23A1.12c |||phenylalanine-tRNA ligase beta subunit
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 589
Score = 68.1 bits (159), Expect = 1e-12
Identities = 30/73 (41%), Positives = 50/73 (68%), Gaps = 1/73 (1%)
Frame = +1
Query: 46 SMPPIINSDHSKITLNTKNVFIECTATDLTKAIVVLDTVVSMFSKYCTNEYEVQQCKVFS 225
S+PPIINS+ SKI+++T+++FI+ TATD TK +V++ + +MFS YC + ++ + S
Sbjct: 219 SLPPIINSEFSKISVDTRDIFIDVTATDKTKLEIVVNMMTTMFSCYCEEPFTIEPVNIIS 278
Query: 226 P-DGTYELYPNFN 261
+G + PN N
Sbjct: 279 EHNGCTRVTPNLN 291
Score = 34.3 bits (75), Expect = 0.016
Identities = 18/52 (34%), Positives = 28/52 (53%), Gaps = 3/52 (5%)
Frame = +3
Query: 279 NVDKANNYIGISEEGDKLASLLSRMCLQTA---HEGSVLRVRVPPTRHDVIH 425
++D N G+S D++ LL+RM L ++ L V VPP R D++H
Sbjct: 298 DIDYLNEACGLSLPEDEICHLLTRMMLTAKPNPNDSKTLLVYVPPLRADILH 349
Score = 27.9 bits (59), Expect = 1.4
Identities = 12/27 (44%), Positives = 17/27 (62%), Gaps = 1/27 (3%)
Frame = +2
Query: 431 DLYEDIAIAYGYNRIARR-PVRAVTSG 508
D+ ED+ IAYGY+ + P +VT G
Sbjct: 352 DIMEDLGIAYGYDNLKHTYPAHSVTFG 378
>SPAC19D5.04 |ptr1||HECT domain|Schizosaccharomyces pombe|chr
1|||Manual
Length = 3227
Score = 35.5 bits (78), Expect = 0.007
Identities = 21/56 (37%), Positives = 28/56 (50%)
Frame = -1
Query: 422 NDVVPRGGXSHAQHGALVRGLETHPGQQRR*LITFFTDPNIVVGFININKFFSILK 255
N P G ++HGAL+R L PG RR +I DP+ F+N N +LK
Sbjct: 2024 NTETPSGADILSRHGALLRDLFPLPGLSRRVMIINSNDPSRSRPFLNNNASEGLLK 2079
>SPAC4H3.12c |||sequence orphan|Schizosaccharomyces pombe|chr
1|||Manual
Length = 101
Score = 27.1 bits (57), Expect = 2.5
Identities = 15/48 (31%), Positives = 21/48 (43%), Gaps = 3/48 (6%)
Frame = +1
Query: 64 NSDHSKITLNTKNVFIECTATDLTKAIVVLDTVVSMFSKY---CTNEY 198
N H + N + C L K I+ L+T+V +F Y NEY
Sbjct: 7 NYSHKNFNVTNNNFQVSCFNPILLKIILFLNTIVCIFYVYKIALCNEY 54
>SPCC162.09c |hmg1||3-hydroxy-3-methylglutaryl-CoA
reductase|Schizosaccharomyces pombe|chr 3|||Manual
Length = 1053
Score = 26.6 bits (56), Expect = 3.3
Identities = 15/41 (36%), Positives = 22/41 (53%)
Frame = +1
Query: 109 IECTATDLTKAIVVLDTVVSMFSKYCTNEYEVQQCKVFSPD 231
+E D+T+A+V+ TVVS S+ T E C V+ D
Sbjct: 633 LERVLKDVTRAVVIRRTVVSRSSR--TKTLESSNCPVYHYD 671
>SPCC14G10.02 ||SPCC18B5.13|ribosome biogenesis protein
Urb1|Schizosaccharomyces pombe|chr 3|||Manual
Length = 1568
Score = 25.8 bits (54), Expect = 5.7
Identities = 16/41 (39%), Positives = 21/41 (51%), Gaps = 1/41 (2%)
Frame = +1
Query: 79 KITLNTKNVF-IECTATDLTKAIVVLDTVVSMFSKYCTNEY 198
KI NT N + E DL K L+T + S YC+NE+
Sbjct: 1286 KIAANTVNQYPSEWEKIDL-KVFDDLETTEGLLSSYCSNEW 1325
>SPAC167.06c |mug143|SPAC57A7.02c|sequence
orphan|Schizosaccharomyces pombe|chr 1|||Manual
Length = 121
Score = 25.4 bits (53), Expect = 7.5
Identities = 12/25 (48%), Positives = 16/25 (64%)
Frame = +3
Query: 309 ISEEGDKLASLLSRMCLQTAHEGSV 383
+ EE DKL SLL + ++TA E V
Sbjct: 57 VDEEEDKLNSLLGNIKVRTAQEDGV 81
>SPBC29A3.09c |||AAA family ATPase Gcn20 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 736
Score = 25.4 bits (53), Expect = 7.5
Identities = 13/50 (26%), Positives = 22/50 (44%)
Frame = +1
Query: 142 IVVLDTVVSMFSKYCTNEYEVQQCKVFSPDGTYELYPNFNIEKNLLMLIK 291
++++ V K CT+ ++ V DGT Y F + + M IK
Sbjct: 686 VILVSHDVDFLDKTCTSIWQCDHNVVSKFDGTISQYKKFCLSQQPTMTIK 735
>SPAC144.05 |||ATP-dependent DNA helicase|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 1375
Score = 25.4 bits (53), Expect = 7.5
Identities = 10/25 (40%), Positives = 16/25 (64%)
Frame = -2
Query: 244 IRKFHQERKLYIVVLHTRSCSISRT 170
+++F +ER L ++ LH RS S T
Sbjct: 1243 LKRFKEERSLQVLTLHARSQSSGLT 1267
>SPAC13F5.03c |||glycerol dehydrogenase |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 450
Score = 25.4 bits (53), Expect = 7.5
Identities = 9/38 (23%), Positives = 21/38 (55%)
Frame = +1
Query: 217 VFSPDGTYELYPNFNIEKNLLMLIKPTTILGSVKKVIS 330
+++PDG ++ Y + + NL+ + + V+ +IS
Sbjct: 191 IYTPDGQFQKYSFYPLNPNLIFIDTDVIVRAPVRFLIS 228
>SPCC132.04c |||NAD-dependent glutamate dehydrogenase
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 1106
Score = 25.0 bits (52), Expect = 9.9
Identities = 11/33 (33%), Positives = 18/33 (54%)
Frame = +1
Query: 160 VVSMFSKYCTNEYEVQQCKVFSPDGTYELYPNF 258
++ + K CTNE +V ++F T+ L NF
Sbjct: 528 LMDLIQKTCTNEEDVSVMEMFVKFNTHLLKTNF 560
>SPAPJ698.03c |prp12|sap130|U2 snRNP-associated protein Sap130
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1206
Score = 25.0 bits (52), Expect = 9.9
Identities = 9/13 (69%), Positives = 10/13 (76%)
Frame = -2
Query: 652 IFKHPNFPXEYVL 614
I KHPNFP Y+L
Sbjct: 806 IVKHPNFPVLYIL 818
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,392,856
Number of Sequences: 5004
Number of extensions: 42050
Number of successful extensions: 111
Number of sequences better than 10.0: 11
Number of HSP's better than 10.0 without gapping: 108
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 111
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 307866294
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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