BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= NV060463.seq
(671 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At1g72550.2 68414.m08390 tRNA synthetase beta subunit family pro... 93 1e-19
At1g72550.1 68414.m08389 tRNA synthetase beta subunit family pro... 93 1e-19
At5g43470.2 68418.m05315 disease resistance protein (CC-NBS-LRR ... 29 3.7
At5g43470.1 68418.m05314 disease resistance protein (CC-NBS-LRR ... 29 3.7
At4g12440.1 68417.m01968 adenine phosphoribosyltransferase, puta... 29 3.7
At5g48620.1 68418.m06013 disease resistance protein (CC-NBS-LRR ... 27 8.6
>At1g72550.2 68414.m08390 tRNA synthetase beta subunit family
protein contains Pfam profiles: PF03484
phenylalanine-tRNA synthetase, B5 domain, PF03483 B3/4
domain; an isoform contains a non-consensus TG acceptor
splice site at a terminal exon.
Length = 584
Score = 93.5 bits (222), Expect = 1e-19
Identities = 41/75 (54%), Positives = 57/75 (76%)
Frame = +1
Query: 40 LLSMPPIINSDHSKITLNTKNVFIECTATDLTKAIVVLDTVVSMFSKYCTNEYEVQQCKV 219
+LS+PPIIN HS ITL TKNVFIECTATDLTKA +VL+T+V+ FS++C ++E++ +V
Sbjct: 231 VLSLPPIINGAHSAITLQTKNVFIECTATDLTKAKIVLNTMVTTFSEFCARKFEIEPVEV 290
Query: 220 FSPDGTYELYPNFNI 264
DG +YP+ +
Sbjct: 291 TYDDGKSYIYPDLAV 305
Score = 35.1 bits (77), Expect = 0.043
Identities = 19/46 (41%), Positives = 29/46 (63%), Gaps = 5/46 (10%)
Frame = +3
Query: 303 IGISEEGDKLASLLSRMCLQTAHEGS-----VLRVRVPPTRHDVIH 425
IG+S + +++ SLL+RM LQ S ++V VPP+R DV+H
Sbjct: 319 IGVSLKVEQVTSLLTRMQLQAEQAKSSDNQCAIKVHVPPSRSDVLH 364
Score = 28.7 bits (61), Expect = 3.7
Identities = 11/18 (61%), Positives = 14/18 (77%)
Frame = +2
Query: 431 DLYEDIAIAYGYNRIARR 484
D+ ED+AIAYG+N I R
Sbjct: 367 DVMEDVAIAYGFNNIPTR 384
>At1g72550.1 68414.m08389 tRNA synthetase beta subunit family
protein contains Pfam profiles: PF03484
phenylalanine-tRNA synthetase, B5 domain, PF03483 B3/4
domain; an isoform contains a non-consensus TG acceptor
splice site at a terminal exon.
Length = 598
Score = 93.5 bits (222), Expect = 1e-19
Identities = 41/75 (54%), Positives = 57/75 (76%)
Frame = +1
Query: 40 LLSMPPIINSDHSKITLNTKNVFIECTATDLTKAIVVLDTVVSMFSKYCTNEYEVQQCKV 219
+LS+PPIIN HS ITL TKNVFIECTATDLTKA +VL+T+V+ FS++C ++E++ +V
Sbjct: 231 VLSLPPIINGAHSAITLQTKNVFIECTATDLTKAKIVLNTMVTTFSEFCARKFEIEPVEV 290
Query: 220 FSPDGTYELYPNFNI 264
DG +YP+ +
Sbjct: 291 TYDDGKSYIYPDLAV 305
Score = 35.1 bits (77), Expect = 0.043
Identities = 19/46 (41%), Positives = 29/46 (63%), Gaps = 5/46 (10%)
Frame = +3
Query: 303 IGISEEGDKLASLLSRMCLQTAHEGS-----VLRVRVPPTRHDVIH 425
IG+S + +++ SLL+RM LQ S ++V VPP+R DV+H
Sbjct: 319 IGVSLKVEQVTSLLTRMQLQAEQAKSSDNQCAIKVHVPPSRSDVLH 364
Score = 28.7 bits (61), Expect = 3.7
Identities = 11/18 (61%), Positives = 14/18 (77%)
Frame = +2
Query: 431 DLYEDIAIAYGYNRIARR 484
D+ ED+AIAYG+N I R
Sbjct: 367 DVMEDVAIAYGFNNIPTR 384
>At5g43470.2 68418.m05315 disease resistance protein (CC-NBS-LRR
class), putative domain signature CC-NBS-LRR exists,
suggestive of a disease resistance protein. Closest
Col-0 homolog to RPP8
Length = 908
Score = 28.7 bits (61), Expect = 3.7
Identities = 16/65 (24%), Positives = 34/65 (52%), Gaps = 3/65 (4%)
Frame = +3
Query: 261 YREELIN---VDKANNYIGISEEGDKLASLLSRMCLQTAHEGSVLRVRVPPTRHDVIHAW 431
Y EEL+ V +NY+ + ++ ++ +CL A E + L++ + PT I+A
Sbjct: 466 YLEELVRRNLVIADDNYLSWQSKYCQMHDMMREVCLSKAKEENFLQIIIDPTCTSTINAQ 525
Query: 432 TSTKT 446
+ +++
Sbjct: 526 SPSRS 530
>At5g43470.1 68418.m05314 disease resistance protein (CC-NBS-LRR
class), putative domain signature CC-NBS-LRR exists,
suggestive of a disease resistance protein. Closest
Col-0 homolog to RPP8
Length = 908
Score = 28.7 bits (61), Expect = 3.7
Identities = 16/65 (24%), Positives = 34/65 (52%), Gaps = 3/65 (4%)
Frame = +3
Query: 261 YREELIN---VDKANNYIGISEEGDKLASLLSRMCLQTAHEGSVLRVRVPPTRHDVIHAW 431
Y EEL+ V +NY+ + ++ ++ +CL A E + L++ + PT I+A
Sbjct: 466 YLEELVRRNLVIADDNYLSWQSKYCQMHDMMREVCLSKAKEENFLQIIIDPTCTSTINAQ 525
Query: 432 TSTKT 446
+ +++
Sbjct: 526 SPSRS 530
>At4g12440.1 68417.m01968 adenine phosphoribosyltransferase,
putative strong similarity to SP|P31166 Adenine
phosphoribosyltransferase 1 (EC 2.4.2.7) (APRT)
{Arabidopsis thaliana}; contains Pfam profile PF00156:
Phosphoribosyl transferase domain
Length = 178
Score = 28.7 bits (61), Expect = 3.7
Identities = 17/54 (31%), Positives = 24/54 (44%)
Frame = +2
Query: 434 LYEDIAIAYGYNRIARRPVRAVTSGDRTPPTNXTEXAGXNECARXRATLKRFXF 595
++E+ + YG +R+ V AV SGDR + G CA K F F
Sbjct: 99 IFEEYELEYGSDRLEMH-VEAVDSGDRALVVDDLIATGGTLCAAMNLLSKFFSF 151
>At5g48620.1 68418.m06013 disease resistance protein (CC-NBS-LRR
class), putative domain signature CC-NBS-LRR exists,
suggestive of a disease resistance protein.
Length = 908
Score = 27.5 bits (58), Expect = 8.6
Identities = 16/65 (24%), Positives = 32/65 (49%), Gaps = 3/65 (4%)
Frame = +3
Query: 261 YREELIN---VDKANNYIGISEEGDKLASLLSRMCLQTAHEGSVLRVRVPPTRHDVIHAW 431
Y EEL+ V N Y+ + ++ ++ +CL A E + L++ PT I+A
Sbjct: 466 YLEELVRRNLVIADNRYLSLEFNFCQMHDMMREVCLSKAKEENFLQIIKDPTSTSTINAQ 525
Query: 432 TSTKT 446
+ +++
Sbjct: 526 SPSRS 530
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 12,576,898
Number of Sequences: 28952
Number of extensions: 223224
Number of successful extensions: 512
Number of sequences better than 10.0: 6
Number of HSP's better than 10.0 without gapping: 499
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 510
length of database: 12,070,560
effective HSP length: 78
effective length of database: 9,812,304
effective search space used: 1422784080
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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