BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= NV060462.seq (558 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBC32H8.12c |act1|cps8|actin |Schizosaccharomyces pombe|chr 2||... 202 4e-53 SPAC11H11.06 |arp2|SPAC22F8.01|ARP2/3 actin-organizing complex s... 121 6e-29 SPBC1347.12 |||actin-like protein Arp1 |Schizosaccharomyces pomb... 113 1e-26 SPAC630.03 |arp3|act2|actin-like protein Arp3|Schizosaccharomyce... 103 2e-23 SPBP23A10.08 |alp5|arp4|actin-like protein Arp4|Schizosaccharomy... 73 4e-14 SPBC365.10 |||actin-like protein Arp5 |Schizosaccharomyces pombe... 66 3e-12 SPAC23D3.09 |arp42|arp4|SWI/SNF and RSC complex subunit Arp42|Sc... 60 3e-10 SPAC1071.06 |arp9||SWI/SNF and RSC complex subunit Arp9|Schizosa... 47 2e-06 SPAPB18E9.04c |||sequence orphan|Schizosaccharomyces pombe|chr 1... 30 0.27 SPAC26A3.05 |chc1||clathrin heavy chain Chc1 |Schizosaccharomyce... 29 0.61 SPBC115.01c |rrp46||exosome subunit Rrp46 |Schizosaccharomyces p... 26 3.3 SPBC13E7.09 |vrp1||verprolin|Schizosaccharomyces pombe|chr 2|||M... 26 3.3 SPCC5E4.04 |cut1||separase|Schizosaccharomyces pombe|chr 3|||Manual 26 4.3 SPAC20G8.05c |cdc15||cell division control protein Cdc15|Schizos... 26 4.3 SPBC19C2.11c |||mitochondrial outer membrane protein |Schizosacc... 25 7.5 SPCC550.12 |arp6||actin-like protein Arp6|Schizosaccharomyces po... 25 10.0 SPAC56F8.02 |||AMP binding enzyme |Schizosaccharomyces pombe|chr... 25 10.0 >SPBC32H8.12c |act1|cps8|actin |Schizosaccharomyces pombe|chr 2|||Manual Length = 375 Score = 202 bits (492), Expect = 4e-53 Identities = 93/101 (92%), Positives = 96/101 (95%) Frame = +2 Query: 254 NEAQSKRGILTLKYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKAN 433 +EAQSKRGILTLKYPIEHGIV NWDDMEKIWHHTFYNELRVAPEEHP LLTEAPLNPK+N Sbjct: 56 DEAQSKRGILTLKYPIEHGIVNNWDDMEKIWHHTFYNELRVAPEEHPCLLTEAPLNPKSN 115 Query: 434 REKMTQIMFETFNTXAMYVAIQAVLSLYASGRTTGIVLDSG 556 REKMTQI+FETFN A YVAIQAVLSLYASGRTTGIVLDSG Sbjct: 116 REKMTQIIFETFNAPAFYVAIQAVLSLYASGRTTGIVLDSG 156 Score = 113 bits (273), Expect = 1e-26 Identities = 50/60 (83%), Positives = 56/60 (93%) Frame = +3 Query: 93 DEEVAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGMRHRAK 272 +EE+AALV+DNGSGMCKAGFAGDDAPRAVFPSIVGRPRH G+MVGMGQKDSYVG ++K Sbjct: 2 EEEIAALVIDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHHGIMVGMGQKDSYVGDEAQSK 61 >SPAC11H11.06 |arp2|SPAC22F8.01|ARP2/3 actin-organizing complex subunit Arp2|Schizosaccharomyces pombe|chr 1|||Manual Length = 390 Score = 121 bits (292), Expect = 6e-29 Identities = 51/114 (44%), Positives = 83/114 (72%) Frame = +2 Query: 215 RDGRYGTEGLLCRNEAQSKRGILTLKYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHP 394 + G + ++ +EA++ R +L +KYP+E+GI+ ++++M ++W +TF+ +L++ P Sbjct: 43 KTGNVQIKDVMVGDEAEAVRSLLQVKYPMENGIIRDFEEMNQLWDYTFFEKLKIDPRGRK 102 Query: 395 VLLTEAPLNPKANREKMTQIMFETFNTXAMYVAIQAVLSLYASGRTTGIVLDSG 556 +LLTE P+NP ANREKM + MFE + +YVAIQAVLSLYA G ++G+V+DSG Sbjct: 103 ILLTEPPMNPVANREKMCETMFERYGFGGVYVAIQAVLSLYAQGLSSGVVVDSG 156 Score = 48.0 bits (109), Expect = 9e-07 Identities = 21/35 (60%), Positives = 26/35 (74%) Frame = +3 Query: 99 EVAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRP 203 E A +V+DNG+G K G+A D+ PR FPSIVGRP Sbjct: 2 ESAPIVLDNGTGFVKVGYAKDNFPRFQFPSIVGRP 36 >SPBC1347.12 |||actin-like protein Arp1 |Schizosaccharomyces pombe|chr 2|||Manual Length = 379 Score = 113 bits (273), Expect = 1e-26 Identities = 52/102 (50%), Positives = 73/102 (71%), Gaps = 1/102 (0%) Frame = +2 Query: 254 NEAQSKRGILTLKYPIEHGIVTNWDDMEKIWHHTFYNE-LRVAPEEHPVLLTEAPLNPKA 430 +EAQ+ RG+L ++ PIE GI+ NW DME+IW + + ++ L PEEHP+LLTE PL Sbjct: 60 SEAQNLRGLLKIQRPIERGIIQNWSDMEEIWSYIYSDQQLNTLPEEHPLLLTEPPLANIR 119 Query: 431 NREKMTQIMFETFNTXAMYVAIQAVLSLYASGRTTGIVLDSG 556 N+EK+ + +ET N A+ ++Q VL+LYAS RTTGIVL+ G Sbjct: 120 NKEKIAEYFYETLNVPALSFSLQPVLALYASARTTGIVLECG 161 Score = 63.7 bits (148), Expect = 2e-11 Identities = 27/48 (56%), Positives = 35/48 (72%) Frame = +3 Query: 111 LVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVG 254 + +DNGSG KAGFAGDD P+ +FP+ VGR +H+ VM QKD +VG Sbjct: 12 ICIDNGSGFIKAGFAGDDIPKCLFPTCVGRIKHERVMPSSIQKDMFVG 59 >SPAC630.03 |arp3|act2|actin-like protein Arp3|Schizosaccharomyces pombe|chr 1|||Manual Length = 427 Score = 103 bits (246), Expect = 2e-23 Identities = 52/110 (47%), Positives = 67/110 (60%), Gaps = 9/110 (8%) Frame = +2 Query: 254 NEAQSKRGI-LTLKYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKA 430 N+A K +L YPI HG + NWD ME+ W + + LR PE+H LLTE PLNP Sbjct: 78 NDALKKASAGYSLDYPIRHGQIENWDHMERFWQQSLFKYLRCEPEDHYFLLTEPPLNPPE 137 Query: 431 NREKMTQIMFETFNTXAMYVAIQAVLSLYASGRT--------TGIVLDSG 556 NRE +IMFE+FN +Y+A+QAVL+L AS + TG V+DSG Sbjct: 138 NRENTAEIMFESFNCAGLYIAVQAVLALAASWTSSKVTDRSLTGTVVDSG 187 Score = 42.7 bits (96), Expect = 4e-05 Identities = 20/52 (38%), Positives = 32/52 (61%), Gaps = 2/52 (3%) Frame = +3 Query: 111 LVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVG--MGQKDSYVGMR 260 +++DNG+G K G+AG+DAP VFP+++ R G G + K SY+ + Sbjct: 8 IIMDNGTGYSKLGYAGNDAPSYVFPTVIA-TRSAGASSGPAVSSKPSYMASK 58 >SPBP23A10.08 |alp5|arp4|actin-like protein Arp4|Schizosaccharomyces pombe|chr 2|||Manual Length = 433 Score = 72.5 bits (170), Expect = 4e-14 Identities = 34/92 (36%), Positives = 51/92 (55%) Frame = +2 Query: 281 LTLKYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMF 460 + +K I +G V NWD +W + +L+ P EHP+L+TE NP NR K + MF Sbjct: 63 MEIKNAIRNGWVENWDVTVDLWRYGLEQQLKTNPLEHPILITEPFDNPPENRVKTLETMF 122 Query: 461 ETFNTXAMYVAIQAVLSLYASGRTTGIVLDSG 556 E+ A Y+A Q + +ASG+ T ++D G Sbjct: 123 ESLRCPATYLAKQETCAAFASGKGTACLVDIG 154 Score = 40.3 bits (90), Expect = 2e-04 Identities = 16/34 (47%), Positives = 24/34 (70%) Frame = +3 Query: 96 EEVAALVVDNGSGMCKAGFAGDDAPRAVFPSIVG 197 +EV+A+V+D GS + GF+G+D P+ V PS G Sbjct: 9 DEVSAIVIDPGSKWTRIGFSGEDIPKCVLPSYCG 42 >SPBC365.10 |||actin-like protein Arp5 |Schizosaccharomyces pombe|chr 2|||Manual Length = 721 Score = 66.1 bits (154), Expect = 3e-12 Identities = 36/117 (30%), Positives = 64/117 (54%), Gaps = 3/117 (2%) Frame = +2 Query: 215 RDGRYGTEGLLCRNEAQSKRGILTL-KYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEH 391 RD + L N+ + G ++ + P E +++NWD ME++ +TF +L + EH Sbjct: 58 RDRKLSRTSTLVGNDTLIEVGSRSIARSPFERNVISNWDLMEQVLDYTFL-KLGIDRMEH 116 Query: 392 PVLLTEAPLNPKANREKMTQIMFETFNTXAMYVAIQAVLSLYASGR--TTGIVLDSG 556 P+ +TE NP R MT+++FE +N ++ I + S Y + + ++GIVL+ G Sbjct: 117 PICMTEPLANPTYVRSTMTELLFELYNAPSVAYGIDGLFSFYHNTKPSSSGIVLNLG 173 Score = 35.9 bits (79), Expect = 0.004 Identities = 15/32 (46%), Positives = 22/32 (68%) Frame = +3 Query: 111 LVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPR 206 LV+DNGS +AG+ G+ P+ VF ++V R R Sbjct: 27 LVIDNGSWQLRAGWGGEKDPKLVFDNLVSRYR 58 >SPAC23D3.09 |arp42|arp4|SWI/SNF and RSC complex subunit Arp42|Schizosaccharomyces pombe|chr 1|||Manual Length = 430 Score = 59.7 bits (138), Expect = 3e-10 Identities = 24/84 (28%), Positives = 49/84 (58%) Frame = +2 Query: 305 HGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMFETFNTXAM 484 +GI+ +W+ W +L+V P E+ +++TE NP++ R+++ + FE + A Sbjct: 74 NGIIQDWESTLYTWERGLKEKLQVNPTEYAMMITEPSWNPQSVRQQIMEAAFEQLHVPAF 133 Query: 485 YVAIQAVLSLYASGRTTGIVLDSG 556 Y+ QAV +A+ ++T +++D G Sbjct: 134 YLTKQAVCVAFANSKSTALIVDIG 157 Score = 36.7 bits (81), Expect = 0.002 Identities = 14/34 (41%), Positives = 23/34 (67%) Frame = +3 Query: 96 EEVAALVVDNGSGMCKAGFAGDDAPRAVFPSIVG 197 EE+ +LV+D GS + G+AG+++P + PS G Sbjct: 8 EEIPSLVIDPGSCWTRFGYAGEESPMTILPSYYG 41 >SPAC1071.06 |arp9||SWI/SNF and RSC complex subunit Arp9|Schizosaccharomyces pombe|chr 1|||Manual Length = 523 Score = 47.2 bits (107), Expect = 2e-06 Identities = 28/91 (30%), Positives = 43/91 (47%), Gaps = 4/91 (4%) Frame = +2 Query: 296 PIEHGIVTNWDDMEKIWHHTFYNELRVAPEE----HPVLLTEAPLNPKANREKMTQIMFE 463 PI+ G V +W+ ++ W H Y+ L P + +PV L +RE TQ FE Sbjct: 113 PIQRGRVVDWEALKAFWKH-LYSLLLKDPNDTTFRYPVCLVIPTYWSLYDRELATQFFFE 171 Query: 464 TFNTXAMYVAIQAVLSLYASGRTTGIVLDSG 556 +A + ++ LYA G G+V+D G Sbjct: 172 ECQVPGFTIAYEPLMGLYAIGILHGLVIDIG 202 >SPAPB18E9.04c |||sequence orphan|Schizosaccharomyces pombe|chr 1|||Manual Length = 800 Score = 29.9 bits (64), Expect = 0.27 Identities = 19/55 (34%), Positives = 23/55 (41%), Gaps = 1/55 (1%) Frame = -3 Query: 253 PT*ESFCPI-PTITP*WRGLPTIEGNTARGASSPAKPALHIPEPLSTTNAATSSS 92 PT S P+ PT+ P P N S P PL+TTN TS+S Sbjct: 401 PTGNSTTPVTPTVPPTSSSTPLTTTNCTTSTSVPYTSTPVTSTPLATTNCTTSTS 455 >SPAC26A3.05 |chc1||clathrin heavy chain Chc1 |Schizosaccharomyces pombe|chr 1|||Manual Length = 1666 Score = 28.7 bits (61), Expect = 0.61 Identities = 14/42 (33%), Positives = 25/42 (59%), Gaps = 2/42 (4%) Frame = +2 Query: 356 FYNELRVAPEEHPVLLTE--APLNPKANREKMTQIMFETFNT 475 +Y L E+HP+LLT+ A L P+ + ++ +I ++ NT Sbjct: 1399 YYRALNFYLEQHPMLLTDLLAALTPRIDHPRVIRIFEKSENT 1440 >SPBC115.01c |rrp46||exosome subunit Rrp46 |Schizosaccharomyces pombe|chr 2|||Manual Length = 226 Score = 26.2 bits (55), Expect = 3.3 Identities = 10/25 (40%), Positives = 18/25 (72%) Frame = +2 Query: 398 LLTEAPLNPKANREKMTQIMFETFN 472 +L APL+ + +KM +++FET+N Sbjct: 181 VLETAPLHAEEVSKKMKELLFETYN 205 >SPBC13E7.09 |vrp1||verprolin|Schizosaccharomyces pombe|chr 2|||Manual Length = 309 Score = 26.2 bits (55), Expect = 3.3 Identities = 13/31 (41%), Positives = 16/31 (51%) Frame = -3 Query: 232 PIPTITP*WRGLPTIEGNTARGASSPAKPAL 140 P+PT P LPT NT + P+ PAL Sbjct: 74 PVPTGAP---SLPTSSNNTQQAEERPSMPAL 101 >SPCC5E4.04 |cut1||separase|Schizosaccharomyces pombe|chr 3|||Manual Length = 1828 Score = 25.8 bits (54), Expect = 4.3 Identities = 12/23 (52%), Positives = 14/23 (60%) Frame = -1 Query: 138 TYRSHCLLPTRQLLRRTS*ISEI 70 TYR H +PT LL T +SEI Sbjct: 168 TYRDHDAIPTATLLNPTLDVSEI 190 >SPAC20G8.05c |cdc15||cell division control protein Cdc15|Schizosaccharomyces pombe|chr 1|||Manual Length = 927 Score = 25.8 bits (54), Expect = 4.3 Identities = 12/26 (46%), Positives = 14/26 (53%) Frame = -1 Query: 252 LHKSPSVPYRPSRPDGGAFPRSRGTR 175 + K+PS PY SRP A S TR Sbjct: 310 ISKAPSYPYSSSRPSASASLASSPTR 335 >SPBC19C2.11c |||mitochondrial outer membrane protein |Schizosaccharomyces pombe|chr 2|||Manual Length = 452 Score = 25.0 bits (52), Expect = 7.5 Identities = 13/40 (32%), Positives = 22/40 (55%) Frame = -1 Query: 333 MSSQLVTIPCSMGYLRVRIPLLLCASFLHKSPSVPYRPSR 214 +SS +IP +L+ I + L + F + PS+ Y+ SR Sbjct: 157 VSSSFDSIPAIARFLQREIEVQLVSLFQEELPSIIYKMSR 196 >SPCC550.12 |arp6||actin-like protein Arp6|Schizosaccharomyces pombe|chr 3|||Manual Length = 401 Score = 24.6 bits (51), Expect = 10.0 Identities = 10/16 (62%), Positives = 13/16 (81%) Frame = +3 Query: 111 LVVDNGSGMCKAGFAG 158 +V+DNG+ KAGFAG Sbjct: 8 IVLDNGAYHIKAGFAG 23 >SPAC56F8.02 |||AMP binding enzyme |Schizosaccharomyces pombe|chr 1|||Manual Length = 1517 Score = 24.6 bits (51), Expect = 10.0 Identities = 9/25 (36%), Positives = 15/25 (60%) Frame = +3 Query: 156 GDDAPRAVFPSIVGRPRHQGVMVGM 230 G+ PRA F ++ P H G+++ M Sbjct: 502 GNQNPRATFVPLLCLPEHGGMVISM 526 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,419,278 Number of Sequences: 5004 Number of extensions: 51997 Number of successful extensions: 157 Number of sequences better than 10.0: 17 Number of HSP's better than 10.0 without gapping: 136 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 153 length of database: 2,362,478 effective HSP length: 69 effective length of database: 2,017,202 effective search space used: 233995432 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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