BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= NV060461.seq (554 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBC31F10.06c |sar1||ADP-ribosylation factor Sar1|Schizosaccharo... 100 2e-22 SPAC22F3.05c |alp41||ADP-ribosylation factor Alp41|Schizosacchar... 40 3e-04 SPBC4F6.18c |arf1||ADP-ribosylation factor Arf1|Schizosaccharomy... 38 8e-04 SPBC1539.08 |||ADP-ribosylation factor, Arf family|Schizosacchar... 37 0.002 SPBPJ4664.06 |gpt1||UDP-glucose-glycoprotein glucosyltransferase... 28 0.80 SPAC23H3.13c |gpa2|git8|heterotrimeric G protein alpha-2 subunit... 27 2.5 SPBC24C6.06 |gpa1||G-protein alpha subunit |Schizosaccharomyces ... 26 3.2 SPAPJ698.03c |prp12|sap130|U2 snRNP-associated protein Sap130 |S... 26 4.3 SPAC12B10.03 |||WD repeat protein, human WDR20 family|Schizosacc... 25 7.5 SPBC16G5.16 |||transcription factor zf-fungal binuclear cluster ... 25 7.5 SPCC1020.08 |||wybutosine biosynthesis protein Tyw1|Schizosaccha... 25 9.9 SPCC11E10.04 |||mitochondrial ATPase expression protein homolog|... 25 9.9 SPBC1289.12 |usp109||U1 snRNP-associated protein Usp109|Schizosa... 25 9.9 SPAC16.04 |dus3||tRNA dihydrouridine synthase Dus3 |Schizosaccha... 25 9.9 >SPBC31F10.06c |sar1||ADP-ribosylation factor Sar1|Schizosaccharomyces pombe|chr 2|||Manual Length = 190 Score = 100 bits (239), Expect = 2e-22 Identities = 60/116 (51%), Positives = 70/116 (60%), Gaps = 1/116 (0%) Frame = +3 Query: 207 KVRQALVSGX*QCREDTLLHMLKDXRLAQHVPTLHPTSXXLSIGSMRFTTFDLGGHQQAR 386 K + L G + TLLHMLK+ RLA PTLHPTS L+IG++RFTTFDLGGHQQAR Sbjct: 19 KHAKMLFLGLDNAGKTTLLHMLKNDRLAVMQPTLHPTSEELAIGNVRFTTFDLGGHQQAR 78 Query: 387 RXXRDXFPAVDAIVFLVDAC-XPAXSAGVQG*TRLVAH*TRTSVTALWLXLGNKID 551 R RD FP V+ IV+LVD C S L+A V +L LGNKID Sbjct: 79 RLWRDYFPEVNGIVYLVDCCDFERLSESKAELDALLAMEELARVP--FLILGNKID 132 Score = 49.6 bits (113), Expect = 3e-07 Identities = 22/34 (64%), Positives = 25/34 (73%) Frame = +1 Query: 154 MFILDWFTGVLGFLGLWXKSGKLLFLGLDNAGKT 255 MFI++WF L LGL K K+LFLGLDNAGKT Sbjct: 1 MFIINWFYDALAMLGLVNKHAKMLFLGLDNAGKT 34 >SPAC22F3.05c |alp41||ADP-ribosylation factor Alp41|Schizosaccharomyces pombe|chr 1|||Manual Length = 186 Score = 39.5 bits (88), Expect = 3e-04 Identities = 20/76 (26%), Positives = 40/76 (52%) Frame = +3 Query: 216 QALVSGX*QCREDTLLHMLKDXRLAQHVPTLHPTSXXLSIGSMRFTTFDLGGHQQARRXX 395 + L+ G + T+L L + + + PT L + +RFT +D+GG + R Sbjct: 18 RVLLLGLDNAGKTTILKCLLNEDVNEVSPTFGFQIRTLEVEGLRFTIWDIGGQKTLRNFW 77 Query: 396 RDXFPAVDAIVFLVDA 443 ++ F + +AI+++VD+ Sbjct: 78 KNYFESTEAIIWVVDS 93 >SPBC4F6.18c |arf1||ADP-ribosylation factor Arf1|Schizosaccharomyces pombe|chr 2|||Manual Length = 180 Score = 38.3 bits (85), Expect = 8e-04 Identities = 20/74 (27%), Positives = 36/74 (48%) Frame = +3 Query: 222 LVSGX*QCREDTLLHMLKDXRLAQHVPTLHPTSXXLSIGSMRFTTFDLGGHQQARRXXRD 401 L+ G + T+L+ LK + +PT+ + ++ FT +D+GG + R R Sbjct: 21 LMVGLDAAGKTTILYKLKLGEIVTTIPTIGFNVETVEYRNISFTVWDVGGQDKIRPLWRH 80 Query: 402 XFPAVDAIVFLVDA 443 F I+F+VD+ Sbjct: 81 YFQNTQGIIFVVDS 94 >SPBC1539.08 |||ADP-ribosylation factor, Arf family|Schizosaccharomyces pombe|chr 2|||Manual Length = 184 Score = 36.7 bits (81), Expect = 0.002 Identities = 29/116 (25%), Positives = 52/116 (44%), Gaps = 1/116 (0%) Frame = +3 Query: 207 KVRQALVSGX*QCREDTLLHMLKDXRLAQHVPTLHPTSXXLSIGSMRFTTFDLGGHQQAR 386 K + L+ G + T+L+ LK + +PT+ ++ +++F +D+GG + R Sbjct: 20 KEMRILMLGLDAAGKTTILYKLKLNQSVVTIPTVGFNVETVTYKNIKFNVWDVGGQDKIR 79 Query: 387 RXXRDXFPAVDAIVFLVDACXP-AXSAGVQG*TRLVAH*TRTSVTALWLXLGNKID 551 R F ++F+VD+ S Q R+++ R L L L NK D Sbjct: 80 PLWRHYFTGTKGLIFVVDSADSNRISEARQELHRIIS--DREMRDCLLLVLANKQD 133 >SPBPJ4664.06 |gpt1||UDP-glucose-glycoprotein glucosyltransferase Gpt1|Schizosaccharomyces pombe|chr 2|||Manual Length = 1448 Score = 28.3 bits (60), Expect = 0.80 Identities = 17/54 (31%), Positives = 27/54 (50%) Frame = +2 Query: 71 ILQISAKTRDTLPLGLVINIVQFNYIQKCLSSTGSLECWDFLXYGKSQASSCFW 232 IL+ ++ TR+ L + ++ I++ L + GSL D L G S A FW Sbjct: 633 ILRDASLTRNPLVYPSAKSSIKSIDIKRVLENVGSLNHEDILLIGSSNAKYSFW 686 >SPAC23H3.13c |gpa2|git8|heterotrimeric G protein alpha-2 subunit Gpa2 |Schizosaccharomyces pombe|chr 1|||Manual Length = 354 Score = 26.6 bits (56), Expect = 2.5 Identities = 15/54 (27%), Positives = 25/54 (46%) Frame = +3 Query: 276 DXRLAQHVPTLHPTSXXLSIGSMRFTTFDLGGHQQARRXXRDXFPAVDAIVFLV 437 D L TL + ++ ++ FD+GG + RR F V++I+F V Sbjct: 170 DDILRSRNSTLGISEISFTLDHLQIRMFDVGGQRTERRKWIYCFENVNSIIFCV 223 >SPBC24C6.06 |gpa1||G-protein alpha subunit |Schizosaccharomyces pombe|chr 2|||Manual Length = 407 Score = 26.2 bits (55), Expect = 3.2 Identities = 12/27 (44%), Positives = 16/27 (59%) Frame = +3 Query: 357 FDLGGHQQARRXXRDXFPAVDAIVFLV 437 FD+GG + RR F V A++FLV Sbjct: 239 FDVGGQRSERRKWIHCFENVTALLFLV 265 >SPAPJ698.03c |prp12|sap130|U2 snRNP-associated protein Sap130 |Schizosaccharomyces pombe|chr 1|||Manual Length = 1206 Score = 25.8 bits (54), Expect = 4.3 Identities = 9/29 (31%), Positives = 20/29 (68%) Frame = +2 Query: 62 QRNILQISAKTRDTLPLGLVINIVQFNYI 148 Q N++++ K DT+PL + +NI++ ++ Sbjct: 338 QGNVVELRLKYFDTVPLAVQLNILKTGFL 366 >SPAC12B10.03 |||WD repeat protein, human WDR20 family|Schizosaccharomyces pombe|chr 1|||Manual Length = 543 Score = 25.0 bits (52), Expect = 7.5 Identities = 12/32 (37%), Positives = 19/32 (59%), Gaps = 3/32 (9%) Frame = -1 Query: 227 NKSLPDFXHXPRNPNTPVNQ---SRINIFEYN 141 ++S P P +PNTPV + RINI +++ Sbjct: 34 SRSTPQITGSPLDPNTPVKRLFFMRINIAQFS 65 >SPBC16G5.16 |||transcription factor zf-fungal binuclear cluster type |Schizosaccharomyces pombe|chr 2|||Manual Length = 827 Score = 25.0 bits (52), Expect = 7.5 Identities = 10/21 (47%), Positives = 13/21 (61%) Frame = -1 Query: 227 NKSLPDFXHXPRNPNTPVNQS 165 N LP F P + ++PVNQS Sbjct: 79 NLKLPSFLAPPNDKDSPVNQS 99 >SPCC1020.08 |||wybutosine biosynthesis protein Tyw1|Schizosaccharomyces pombe|chr 3|||Manual Length = 688 Score = 24.6 bits (51), Expect = 9.9 Identities = 11/32 (34%), Positives = 20/32 (62%) Frame = -3 Query: 330 TVPRM*DVK*VHVEQVXHLSTCVRVCLPCIVK 235 TV RM VK ++EQ+ + +R+ +PC ++ Sbjct: 511 TVYRMTLVKGFNMEQIKEYTELIRLGVPCFIE 542 >SPCC11E10.04 |||mitochondrial ATPase expression protein homolog|Schizosaccharomyces pombe|chr 3|||Manual Length = 443 Score = 24.6 bits (51), Expect = 9.9 Identities = 12/41 (29%), Positives = 21/41 (51%) Frame = +2 Query: 74 LQISAKTRDTLPLGLVINIVQFNYIQKCLSSTGSLECWDFL 196 LQI +D LG+ NIV + +I + TG+ +++ Sbjct: 113 LQIKLILQDADRLGIQFNIVDYTHILRVAMYTGNKHILEYI 153 >SPBC1289.12 |usp109||U1 snRNP-associated protein Usp109|Schizosaccharomyces pombe|chr 2|||Manual Length = 352 Score = 24.6 bits (51), Expect = 9.9 Identities = 10/27 (37%), Positives = 16/27 (59%) Frame = -1 Query: 251 FPALSXPRNKSLPDFXHXPRNPNTPVN 171 +PAL+ P S+ D + P P +P+N Sbjct: 260 WPALAAPVYPSMKDVPNNPFTPFSPIN 286 >SPAC16.04 |dus3||tRNA dihydrouridine synthase Dus3 |Schizosaccharomyces pombe|chr 1|||Manual Length = 617 Score = 24.6 bits (51), Expect = 9.9 Identities = 11/39 (28%), Positives = 19/39 (48%) Frame = +2 Query: 359 RPRWASAGASXVARLXPRGGRHSVPGRRVXTGXVCRSPR 475 +P WA R RGGR + G ++ TG + ++ + Sbjct: 281 QPEWALVRGLNYEREMMRGGRRGILGVQLATGKLWQATK 319 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 1,860,796 Number of Sequences: 5004 Number of extensions: 30669 Number of successful extensions: 67 Number of sequences better than 10.0: 14 Number of HSP's better than 10.0 without gapping: 62 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 64 length of database: 2,362,478 effective HSP length: 69 effective length of database: 2,017,202 effective search space used: 231978230 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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