Bombyx BLAST Result against spombe

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= NV060461.seq
         (554 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPBC31F10.06c |sar1||ADP-ribosylation factor Sar1|Schizosaccharo...   100   2e-22
SPAC22F3.05c |alp41||ADP-ribosylation factor Alp41|Schizosacchar...    40   3e-04
SPBC4F6.18c |arf1||ADP-ribosylation factor Arf1|Schizosaccharomy...    38   8e-04
SPBC1539.08 |||ADP-ribosylation factor, Arf family|Schizosacchar...    37   0.002
SPBPJ4664.06 |gpt1||UDP-glucose-glycoprotein glucosyltransferase...    28   0.80 
SPAC23H3.13c |gpa2|git8|heterotrimeric G protein alpha-2 subunit...    27   2.5  
SPBC24C6.06 |gpa1||G-protein alpha subunit |Schizosaccharomyces ...    26   3.2  
SPAPJ698.03c |prp12|sap130|U2 snRNP-associated protein Sap130 |S...    26   4.3  
SPAC12B10.03 |||WD repeat protein, human WDR20 family|Schizosacc...    25   7.5  
SPBC16G5.16 |||transcription factor zf-fungal binuclear cluster ...    25   7.5  
SPCC1020.08 |||wybutosine biosynthesis protein Tyw1|Schizosaccha...    25   9.9  
SPCC11E10.04 |||mitochondrial ATPase expression protein homolog|...    25   9.9  
SPBC1289.12 |usp109||U1 snRNP-associated protein Usp109|Schizosa...    25   9.9  
SPAC16.04 |dus3||tRNA dihydrouridine synthase Dus3 |Schizosaccha...    25   9.9  

>SPBC31F10.06c |sar1||ADP-ribosylation factor
           Sar1|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 190

 Score =  100 bits (239), Expect = 2e-22
 Identities = 60/116 (51%), Positives = 70/116 (60%), Gaps = 1/116 (0%)
 Frame = +3

Query: 207 KVRQALVSGX*QCREDTLLHMLKDXRLAQHVPTLHPTSXXLSIGSMRFTTFDLGGHQQAR 386
           K  + L  G     + TLLHMLK+ RLA   PTLHPTS  L+IG++RFTTFDLGGHQQAR
Sbjct: 19  KHAKMLFLGLDNAGKTTLLHMLKNDRLAVMQPTLHPTSEELAIGNVRFTTFDLGGHQQAR 78

Query: 387 RXXRDXFPAVDAIVFLVDAC-XPAXSAGVQG*TRLVAH*TRTSVTALWLXLGNKID 551
           R  RD FP V+ IV+LVD C     S        L+A      V   +L LGNKID
Sbjct: 79  RLWRDYFPEVNGIVYLVDCCDFERLSESKAELDALLAMEELARVP--FLILGNKID 132



 Score = 49.6 bits (113), Expect = 3e-07
 Identities = 22/34 (64%), Positives = 25/34 (73%)
 Frame = +1

Query: 154 MFILDWFTGVLGFLGLWXKSGKLLFLGLDNAGKT 255
           MFI++WF   L  LGL  K  K+LFLGLDNAGKT
Sbjct: 1   MFIINWFYDALAMLGLVNKHAKMLFLGLDNAGKT 34


>SPAC22F3.05c |alp41||ADP-ribosylation factor
           Alp41|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 186

 Score = 39.5 bits (88), Expect = 3e-04
 Identities = 20/76 (26%), Positives = 40/76 (52%)
 Frame = +3

Query: 216 QALVSGX*QCREDTLLHMLKDXRLAQHVPTLHPTSXXLSIGSMRFTTFDLGGHQQARRXX 395
           + L+ G     + T+L  L +  + +  PT       L +  +RFT +D+GG +  R   
Sbjct: 18  RVLLLGLDNAGKTTILKCLLNEDVNEVSPTFGFQIRTLEVEGLRFTIWDIGGQKTLRNFW 77

Query: 396 RDXFPAVDAIVFLVDA 443
           ++ F + +AI+++VD+
Sbjct: 78  KNYFESTEAIIWVVDS 93


>SPBC4F6.18c |arf1||ADP-ribosylation factor Arf1|Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 180

 Score = 38.3 bits (85), Expect = 8e-04
 Identities = 20/74 (27%), Positives = 36/74 (48%)
 Frame = +3

Query: 222 LVSGX*QCREDTLLHMLKDXRLAQHVPTLHPTSXXLSIGSMRFTTFDLGGHQQARRXXRD 401
           L+ G     + T+L+ LK   +   +PT+      +   ++ FT +D+GG  + R   R 
Sbjct: 21  LMVGLDAAGKTTILYKLKLGEIVTTIPTIGFNVETVEYRNISFTVWDVGGQDKIRPLWRH 80

Query: 402 XFPAVDAIVFLVDA 443
            F     I+F+VD+
Sbjct: 81  YFQNTQGIIFVVDS 94


>SPBC1539.08 |||ADP-ribosylation factor, Arf
           family|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 184

 Score = 36.7 bits (81), Expect = 0.002
 Identities = 29/116 (25%), Positives = 52/116 (44%), Gaps = 1/116 (0%)
 Frame = +3

Query: 207 KVRQALVSGX*QCREDTLLHMLKDXRLAQHVPTLHPTSXXLSIGSMRFTTFDLGGHQQAR 386
           K  + L+ G     + T+L+ LK  +    +PT+      ++  +++F  +D+GG  + R
Sbjct: 20  KEMRILMLGLDAAGKTTILYKLKLNQSVVTIPTVGFNVETVTYKNIKFNVWDVGGQDKIR 79

Query: 387 RXXRDXFPAVDAIVFLVDACXP-AXSAGVQG*TRLVAH*TRTSVTALWLXLGNKID 551
              R  F     ++F+VD+      S   Q   R+++   R     L L L NK D
Sbjct: 80  PLWRHYFTGTKGLIFVVDSADSNRISEARQELHRIIS--DREMRDCLLLVLANKQD 133


>SPBPJ4664.06 |gpt1||UDP-glucose-glycoprotein glucosyltransferase
           Gpt1|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 1448

 Score = 28.3 bits (60), Expect = 0.80
 Identities = 17/54 (31%), Positives = 27/54 (50%)
 Frame = +2

Query: 71  ILQISAKTRDTLPLGLVINIVQFNYIQKCLSSTGSLECWDFLXYGKSQASSCFW 232
           IL+ ++ TR+ L      + ++   I++ L + GSL   D L  G S A   FW
Sbjct: 633 ILRDASLTRNPLVYPSAKSSIKSIDIKRVLENVGSLNHEDILLIGSSNAKYSFW 686


>SPAC23H3.13c |gpa2|git8|heterotrimeric G protein alpha-2 subunit
           Gpa2 |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 354

 Score = 26.6 bits (56), Expect = 2.5
 Identities = 15/54 (27%), Positives = 25/54 (46%)
 Frame = +3

Query: 276 DXRLAQHVPTLHPTSXXLSIGSMRFTTFDLGGHQQARRXXRDXFPAVDAIVFLV 437
           D  L     TL  +    ++  ++   FD+GG +  RR     F  V++I+F V
Sbjct: 170 DDILRSRNSTLGISEISFTLDHLQIRMFDVGGQRTERRKWIYCFENVNSIIFCV 223


>SPBC24C6.06 |gpa1||G-protein alpha subunit |Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 407

 Score = 26.2 bits (55), Expect = 3.2
 Identities = 12/27 (44%), Positives = 16/27 (59%)
 Frame = +3

Query: 357 FDLGGHQQARRXXRDXFPAVDAIVFLV 437
           FD+GG +  RR     F  V A++FLV
Sbjct: 239 FDVGGQRSERRKWIHCFENVTALLFLV 265


>SPAPJ698.03c |prp12|sap130|U2 snRNP-associated protein Sap130
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 1206

 Score = 25.8 bits (54), Expect = 4.3
 Identities = 9/29 (31%), Positives = 20/29 (68%)
 Frame = +2

Query: 62  QRNILQISAKTRDTLPLGLVINIVQFNYI 148
           Q N++++  K  DT+PL + +NI++  ++
Sbjct: 338 QGNVVELRLKYFDTVPLAVQLNILKTGFL 366


>SPAC12B10.03 |||WD repeat protein, human WDR20
           family|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 543

 Score = 25.0 bits (52), Expect = 7.5
 Identities = 12/32 (37%), Positives = 19/32 (59%), Gaps = 3/32 (9%)
 Frame = -1

Query: 227 NKSLPDFXHXPRNPNTPVNQ---SRINIFEYN 141
           ++S P     P +PNTPV +    RINI +++
Sbjct: 34  SRSTPQITGSPLDPNTPVKRLFFMRINIAQFS 65


>SPBC16G5.16 |||transcription factor zf-fungal binuclear cluster
           type |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 827

 Score = 25.0 bits (52), Expect = 7.5
 Identities = 10/21 (47%), Positives = 13/21 (61%)
 Frame = -1

Query: 227 NKSLPDFXHXPRNPNTPVNQS 165
           N  LP F   P + ++PVNQS
Sbjct: 79  NLKLPSFLAPPNDKDSPVNQS 99


>SPCC1020.08 |||wybutosine biosynthesis protein
           Tyw1|Schizosaccharomyces pombe|chr 3|||Manual
          Length = 688

 Score = 24.6 bits (51), Expect = 9.9
 Identities = 11/32 (34%), Positives = 20/32 (62%)
 Frame = -3

Query: 330 TVPRM*DVK*VHVEQVXHLSTCVRVCLPCIVK 235
           TV RM  VK  ++EQ+   +  +R+ +PC ++
Sbjct: 511 TVYRMTLVKGFNMEQIKEYTELIRLGVPCFIE 542


>SPCC11E10.04 |||mitochondrial ATPase expression protein
           homolog|Schizosaccharomyces pombe|chr 3|||Manual
          Length = 443

 Score = 24.6 bits (51), Expect = 9.9
 Identities = 12/41 (29%), Positives = 21/41 (51%)
 Frame = +2

Query: 74  LQISAKTRDTLPLGLVINIVQFNYIQKCLSSTGSLECWDFL 196
           LQI    +D   LG+  NIV + +I +    TG+    +++
Sbjct: 113 LQIKLILQDADRLGIQFNIVDYTHILRVAMYTGNKHILEYI 153


>SPBC1289.12 |usp109||U1 snRNP-associated protein
           Usp109|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 352

 Score = 24.6 bits (51), Expect = 9.9
 Identities = 10/27 (37%), Positives = 16/27 (59%)
 Frame = -1

Query: 251 FPALSXPRNKSLPDFXHXPRNPNTPVN 171
           +PAL+ P   S+ D  + P  P +P+N
Sbjct: 260 WPALAAPVYPSMKDVPNNPFTPFSPIN 286


>SPAC16.04 |dus3||tRNA dihydrouridine synthase Dus3
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 617

 Score = 24.6 bits (51), Expect = 9.9
 Identities = 11/39 (28%), Positives = 19/39 (48%)
 Frame = +2

Query: 359 RPRWASAGASXVARLXPRGGRHSVPGRRVXTGXVCRSPR 475
           +P WA        R   RGGR  + G ++ TG + ++ +
Sbjct: 281 QPEWALVRGLNYEREMMRGGRRGILGVQLATGKLWQATK 319


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 1,860,796
Number of Sequences: 5004
Number of extensions: 30669
Number of successful extensions: 67
Number of sequences better than 10.0: 14
Number of HSP's better than 10.0 without gapping: 62
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 64
length of database: 2,362,478
effective HSP length: 69
effective length of database: 2,017,202
effective search space used: 231978230
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)