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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= NV060460.seq
         (681 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At3g57290.1 68416.m06377 eukaryotic translation initiation facto...    67   1e-11
At3g62050.1 68416.m06971 expressed protein contains Pfam profile...    29   3.8  
At3g32100.1 68416.m04084 hypothetical protein                          29   3.8  
At1g64320.1 68414.m07289 myosin heavy chain-related similar to m...    28   5.0  
At1g60420.1 68414.m06802 DC1 domain-containing protein contains ...    27   8.7  

>At3g57290.1 68416.m06377 eukaryotic translation initiation factor
           3E / eIF3e (TIF3E1) identical to eukaryotic initiation
           factor 3E subunit [Arabidopsis thaliana]
           gi|12407658|gb|AAG53613
          Length = 441

 Score = 66.9 bits (156), Expect = 1e-11
 Identities = 45/182 (24%), Positives = 86/182 (47%)
 Frame = +3

Query: 87  SYSKFDLTFKIGQYLDRHLVFPLLEFLAAKETYDQSELLQAKLEILSKTNMIDYVTTSEE 266
           S   +DLT  I   LDRHLVFP+ EFL  ++ Y   ++L++K+++L++TNM+DY     +
Sbjct: 4   SKQNYDLTPLIAPNLDRHLVFPIFEFLQERQLYPDEQILKSKIQLLNQTNMVDYAMDIHK 63

Query: 267 CSILKKTLLKKLYSGEV*FFRNCRNFKMQ*SQY*DSCKEMM**XTIXTMRDPKTLIXHLS 446
                +   +++           ++ +   +        ++    +  +R  K     + 
Sbjct: 64  SLYHTEDAPQEMVERRTEVVARLKSLEEAAAPL---VSFLLNPNAVQELRADKQYNLQM- 119

Query: 447 TNKEYEFKIEMIDSMYRLAKYAMNVXIMWSQHHIFTFGQLVMSPTDKNYLSVLWGKLASE 626
             + Y+   + I+++Y+ AK+       +S    + +    +    +  LS LWGKLASE
Sbjct: 120 LKERYQIGPDQIEALYQYAKFQFECG-NYSGAADYLYQYRTLCSNLERSLSALWGKLASE 178

Query: 627 IL 632
           IL
Sbjct: 179 IL 180



 Score = 45.2 bits (102), Expect = 4e-05
 Identities = 26/103 (25%), Positives = 50/103 (48%)
 Frame = +2

Query: 257 IRRMLYPEEDTPEEIIQRRGVVLSELQELQDAVEPVLRLMQRDDVMXNNXXHEGS*NSYX 436
           I + LY  ED P+E+++RR  V++ L+ L++A  P++  +   + +      +     Y 
Sbjct: 61  IHKSLYHTEDAPQEMVERRTEVVARLKSLEEAAAPLVSFLLNPNAVQELRADK----QYN 116

Query: 437 PSFDKQGV*V*N*NDR*HVSFGQIRYECGNYVESASYLYFWST 565
               K+   +          + + ++ECGNY  +A YLY + T
Sbjct: 117 LQMLKERYQIGPDQIEALYQYAKFQFECGNYSGAADYLYQYRT 159


>At3g62050.1 68416.m06971 expressed protein contains Pfam profile
           PF04396: Protein of unknown function, DUF537
          Length = 157

 Score = 28.7 bits (61), Expect = 3.8
 Identities = 14/41 (34%), Positives = 25/41 (60%), Gaps = 1/41 (2%)
 Frame = -3

Query: 349 ILKFLQFRKNYTSPLYNFFRSVFF-RIEHSSDVVT*SIMLV 230
           +++ L+ +KNY   +YNF   V++ + EHS      SI+L+
Sbjct: 117 VVQLLKSKKNYNFIIYNFILYVYYQKTEHSYLAPIPSILLI 157


>At3g32100.1 68416.m04084 hypothetical protein
          Length = 79

 Score = 28.7 bits (61), Expect = 3.8
 Identities = 16/47 (34%), Positives = 26/47 (55%)
 Frame = +3

Query: 162 FLAAKETYDQSELLQAKLEILSKTNMIDYVTTSEECSILKKTLLKKL 302
           FL++ +    SE+L + +   S    ++  T+ E CSI KKT LK +
Sbjct: 9   FLSSSQALSSSEMLVSGMTTPSSKIAVED-TSVERCSITKKTCLKPI 54


>At1g64320.1 68414.m07289 myosin heavy chain-related similar to
           myosin heavy chain (GI:4249703) [Rana catesbeiana];
           similar to smooth muscle myosin heavy chain SM2
           (GI:2352945) [Homo sapiens]
          Length = 476

 Score = 28.3 bits (60), Expect = 5.0
 Identities = 14/38 (36%), Positives = 23/38 (60%)
 Frame = +3

Query: 195 ELLQAKLEILSKTNMIDYVTTSEECSILKKTLLKKLYS 308
           E+  AKL +  K + ++ +T   ECS  ++TLLK+  S
Sbjct: 358 EMESAKLWVSEKKSEVETLTAKLECSEAQETLLKEKLS 395


>At1g60420.1 68414.m06802 DC1 domain-containing protein contains
           Pfam domain PF03107: DC1 domain
          Length = 578

 Score = 27.5 bits (58), Expect = 8.7
 Identities = 13/44 (29%), Positives = 22/44 (50%)
 Frame = +1

Query: 250 LLHQKNALS*RRHS*RNYTAERCSSFGTAGTSRCSRASIETHAK 381
           +LH+++ L   R   + YT ++C   GT  +  C     + HAK
Sbjct: 501 VLHEEHELELTRV--QVYTCDKCEEEGTIWSYHCDECDFDLHAK 542


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 13,907,323
Number of Sequences: 28952
Number of extensions: 266965
Number of successful extensions: 666
Number of sequences better than 10.0: 5
Number of HSP's better than 10.0 without gapping: 650
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 666
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1438152744
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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