BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= NV060460.seq (681 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At3g57290.1 68416.m06377 eukaryotic translation initiation facto... 67 1e-11 At3g62050.1 68416.m06971 expressed protein contains Pfam profile... 29 3.8 At3g32100.1 68416.m04084 hypothetical protein 29 3.8 At1g64320.1 68414.m07289 myosin heavy chain-related similar to m... 28 5.0 At1g60420.1 68414.m06802 DC1 domain-containing protein contains ... 27 8.7 >At3g57290.1 68416.m06377 eukaryotic translation initiation factor 3E / eIF3e (TIF3E1) identical to eukaryotic initiation factor 3E subunit [Arabidopsis thaliana] gi|12407658|gb|AAG53613 Length = 441 Score = 66.9 bits (156), Expect = 1e-11 Identities = 45/182 (24%), Positives = 86/182 (47%) Frame = +3 Query: 87 SYSKFDLTFKIGQYLDRHLVFPLLEFLAAKETYDQSELLQAKLEILSKTNMIDYVTTSEE 266 S +DLT I LDRHLVFP+ EFL ++ Y ++L++K+++L++TNM+DY + Sbjct: 4 SKQNYDLTPLIAPNLDRHLVFPIFEFLQERQLYPDEQILKSKIQLLNQTNMVDYAMDIHK 63 Query: 267 CSILKKTLLKKLYSGEV*FFRNCRNFKMQ*SQY*DSCKEMM**XTIXTMRDPKTLIXHLS 446 + +++ ++ + + ++ + +R K + Sbjct: 64 SLYHTEDAPQEMVERRTEVVARLKSLEEAAAPL---VSFLLNPNAVQELRADKQYNLQM- 119 Query: 447 TNKEYEFKIEMIDSMYRLAKYAMNVXIMWSQHHIFTFGQLVMSPTDKNYLSVLWGKLASE 626 + Y+ + I+++Y+ AK+ +S + + + + LS LWGKLASE Sbjct: 120 LKERYQIGPDQIEALYQYAKFQFECG-NYSGAADYLYQYRTLCSNLERSLSALWGKLASE 178 Query: 627 IL 632 IL Sbjct: 179 IL 180 Score = 45.2 bits (102), Expect = 4e-05 Identities = 26/103 (25%), Positives = 50/103 (48%) Frame = +2 Query: 257 IRRMLYPEEDTPEEIIQRRGVVLSELQELQDAVEPVLRLMQRDDVMXNNXXHEGS*NSYX 436 I + LY ED P+E+++RR V++ L+ L++A P++ + + + + Y Sbjct: 61 IHKSLYHTEDAPQEMVERRTEVVARLKSLEEAAAPLVSFLLNPNAVQELRADK----QYN 116 Query: 437 PSFDKQGV*V*N*NDR*HVSFGQIRYECGNYVESASYLYFWST 565 K+ + + + ++ECGNY +A YLY + T Sbjct: 117 LQMLKERYQIGPDQIEALYQYAKFQFECGNYSGAADYLYQYRT 159 >At3g62050.1 68416.m06971 expressed protein contains Pfam profile PF04396: Protein of unknown function, DUF537 Length = 157 Score = 28.7 bits (61), Expect = 3.8 Identities = 14/41 (34%), Positives = 25/41 (60%), Gaps = 1/41 (2%) Frame = -3 Query: 349 ILKFLQFRKNYTSPLYNFFRSVFF-RIEHSSDVVT*SIMLV 230 +++ L+ +KNY +YNF V++ + EHS SI+L+ Sbjct: 117 VVQLLKSKKNYNFIIYNFILYVYYQKTEHSYLAPIPSILLI 157 >At3g32100.1 68416.m04084 hypothetical protein Length = 79 Score = 28.7 bits (61), Expect = 3.8 Identities = 16/47 (34%), Positives = 26/47 (55%) Frame = +3 Query: 162 FLAAKETYDQSELLQAKLEILSKTNMIDYVTTSEECSILKKTLLKKL 302 FL++ + SE+L + + S ++ T+ E CSI KKT LK + Sbjct: 9 FLSSSQALSSSEMLVSGMTTPSSKIAVED-TSVERCSITKKTCLKPI 54 >At1g64320.1 68414.m07289 myosin heavy chain-related similar to myosin heavy chain (GI:4249703) [Rana catesbeiana]; similar to smooth muscle myosin heavy chain SM2 (GI:2352945) [Homo sapiens] Length = 476 Score = 28.3 bits (60), Expect = 5.0 Identities = 14/38 (36%), Positives = 23/38 (60%) Frame = +3 Query: 195 ELLQAKLEILSKTNMIDYVTTSEECSILKKTLLKKLYS 308 E+ AKL + K + ++ +T ECS ++TLLK+ S Sbjct: 358 EMESAKLWVSEKKSEVETLTAKLECSEAQETLLKEKLS 395 >At1g60420.1 68414.m06802 DC1 domain-containing protein contains Pfam domain PF03107: DC1 domain Length = 578 Score = 27.5 bits (58), Expect = 8.7 Identities = 13/44 (29%), Positives = 22/44 (50%) Frame = +1 Query: 250 LLHQKNALS*RRHS*RNYTAERCSSFGTAGTSRCSRASIETHAK 381 +LH+++ L R + YT ++C GT + C + HAK Sbjct: 501 VLHEEHELELTRV--QVYTCDKCEEEGTIWSYHCDECDFDLHAK 542 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 13,907,323 Number of Sequences: 28952 Number of extensions: 266965 Number of successful extensions: 666 Number of sequences better than 10.0: 5 Number of HSP's better than 10.0 without gapping: 650 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 666 length of database: 12,070,560 effective HSP length: 79 effective length of database: 9,783,352 effective search space used: 1438152744 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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