BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= NV060455.seq
(672 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAP32A8.03c |||ubiquitin-protein ligase E3 |Schizosaccharomyces... 34 0.021
SPAC12B10.01c ||SPAC31F12.02c, SPAC637.15c|ubiquitin-protein lig... 26 5.7
SPAC23D3.06c |nup146||nucleoporin Nup146|Schizosaccharomyces pom... 25 7.5
SPAC12G12.14c |pfs2||WD repeat protein Pfs2|Schizosaccharomyces ... 25 9.9
>SPAP32A8.03c |||ubiquitin-protein ligase E3 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 513
Score = 33.9 bits (74), Expect = 0.021
Identities = 13/25 (52%), Positives = 18/25 (72%)
Frame = +2
Query: 512 LVGAPGDYVFGGEGLDAVVTQLLGQ 586
L G PGDY +G GLD +++QL+ Q
Sbjct: 338 LSGNPGDYAWGARGLDDIISQLMEQ 362
>SPAC12B10.01c ||SPAC31F12.02c, SPAC637.15c|ubiquitin-protein ligase
E3 |Schizosaccharomyces pombe|chr 1|||Manual
Length = 1647
Score = 25.8 bits (54), Expect = 5.7
Identities = 9/28 (32%), Positives = 17/28 (60%)
Frame = -1
Query: 93 NFQQKFNNRHFFEHSQSMDFLKKKKKTR 10
N + K +N+H S+ + F KK++ T+
Sbjct: 30 NLRSKSDNKHRLNTSEELPFTKKRRATK 57
>SPAC23D3.06c |nup146||nucleoporin Nup146|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 1325
Score = 25.4 bits (53), Expect = 7.5
Identities = 11/32 (34%), Positives = 17/32 (53%)
Frame = +2
Query: 569 TQLLGQFGELRAXXSTTGSASPHYHRRYSRRD 664
+ ++ QF L S+T S HY R + R+D
Sbjct: 1277 SDMMTQFRALGISQSSTKQVSAHYLREFERQD 1308
>SPAC12G12.14c |pfs2||WD repeat protein Pfs2|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 509
Score = 25.0 bits (52), Expect = 9.9
Identities = 24/105 (22%), Positives = 39/105 (37%), Gaps = 6/105 (5%)
Frame = -1
Query: 315 NHHPLHSPIAL*NH*-HNMGMLILQNIFKFYITPVAEKP*C-----WSSFYHCVSHVKLI 154
NH+ L P L H + + + + P AE P WS YH + H+
Sbjct: 318 NHYSLDEPPLLSQQKYHEKHPNVTLSASSYLLYPTAEIPFAHDLGIWSMQYHPLGHLLCT 377
Query: 153 NTTAPINTYYLNSIPKDCALNFQQKFNNRHFFEHSQSMDFLKKKK 19
+ ++ S P D ++ +RH QS L ++K
Sbjct: 378 GSNDKTTRFWSRSRPDD-----KESTMDRHHLGEEQSEAMLSQRK 417
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,600,811
Number of Sequences: 5004
Number of extensions: 49688
Number of successful extensions: 123
Number of sequences better than 10.0: 4
Number of HSP's better than 10.0 without gapping: 119
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 123
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 307866294
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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