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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= NV060455.seq
         (672 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPAP32A8.03c |||ubiquitin-protein ligase E3 |Schizosaccharomyces...    34   0.021
SPAC12B10.01c ||SPAC31F12.02c, SPAC637.15c|ubiquitin-protein lig...    26   5.7  
SPAC23D3.06c |nup146||nucleoporin Nup146|Schizosaccharomyces pom...    25   7.5  
SPAC12G12.14c |pfs2||WD repeat protein Pfs2|Schizosaccharomyces ...    25   9.9  

>SPAP32A8.03c |||ubiquitin-protein ligase E3 |Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 513

 Score = 33.9 bits (74), Expect = 0.021
 Identities = 13/25 (52%), Positives = 18/25 (72%)
 Frame = +2

Query: 512 LVGAPGDYVFGGEGLDAVVTQLLGQ 586
           L G PGDY +G  GLD +++QL+ Q
Sbjct: 338 LSGNPGDYAWGARGLDDIISQLMEQ 362


>SPAC12B10.01c ||SPAC31F12.02c, SPAC637.15c|ubiquitin-protein ligase
           E3 |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 1647

 Score = 25.8 bits (54), Expect = 5.7
 Identities = 9/28 (32%), Positives = 17/28 (60%)
 Frame = -1

Query: 93  NFQQKFNNRHFFEHSQSMDFLKKKKKTR 10
           N + K +N+H    S+ + F KK++ T+
Sbjct: 30  NLRSKSDNKHRLNTSEELPFTKKRRATK 57


>SPAC23D3.06c |nup146||nucleoporin Nup146|Schizosaccharomyces
            pombe|chr 1|||Manual
          Length = 1325

 Score = 25.4 bits (53), Expect = 7.5
 Identities = 11/32 (34%), Positives = 17/32 (53%)
 Frame = +2

Query: 569  TQLLGQFGELRAXXSTTGSASPHYHRRYSRRD 664
            + ++ QF  L    S+T   S HY R + R+D
Sbjct: 1277 SDMMTQFRALGISQSSTKQVSAHYLREFERQD 1308


>SPAC12G12.14c |pfs2||WD repeat protein Pfs2|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 509

 Score = 25.0 bits (52), Expect = 9.9
 Identities = 24/105 (22%), Positives = 39/105 (37%), Gaps = 6/105 (5%)
 Frame = -1

Query: 315 NHHPLHSPIAL*NH*-HNMGMLILQNIFKFYITPVAEKP*C-----WSSFYHCVSHVKLI 154
           NH+ L  P  L     H     +  +   + + P AE P       WS  YH + H+   
Sbjct: 318 NHYSLDEPPLLSQQKYHEKHPNVTLSASSYLLYPTAEIPFAHDLGIWSMQYHPLGHLLCT 377

Query: 153 NTTAPINTYYLNSIPKDCALNFQQKFNNRHFFEHSQSMDFLKKKK 19
            +      ++  S P D     ++   +RH     QS   L ++K
Sbjct: 378 GSNDKTTRFWSRSRPDD-----KESTMDRHHLGEEQSEAMLSQRK 417


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,600,811
Number of Sequences: 5004
Number of extensions: 49688
Number of successful extensions: 123
Number of sequences better than 10.0: 4
Number of HSP's better than 10.0 without gapping: 119
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 123
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 307866294
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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