BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= NV060455.seq (672 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAP32A8.03c |||ubiquitin-protein ligase E3 |Schizosaccharomyces... 34 0.021 SPAC12B10.01c ||SPAC31F12.02c, SPAC637.15c|ubiquitin-protein lig... 26 5.7 SPAC23D3.06c |nup146||nucleoporin Nup146|Schizosaccharomyces pom... 25 7.5 SPAC12G12.14c |pfs2||WD repeat protein Pfs2|Schizosaccharomyces ... 25 9.9 >SPAP32A8.03c |||ubiquitin-protein ligase E3 |Schizosaccharomyces pombe|chr 1|||Manual Length = 513 Score = 33.9 bits (74), Expect = 0.021 Identities = 13/25 (52%), Positives = 18/25 (72%) Frame = +2 Query: 512 LVGAPGDYVFGGEGLDAVVTQLLGQ 586 L G PGDY +G GLD +++QL+ Q Sbjct: 338 LSGNPGDYAWGARGLDDIISQLMEQ 362 >SPAC12B10.01c ||SPAC31F12.02c, SPAC637.15c|ubiquitin-protein ligase E3 |Schizosaccharomyces pombe|chr 1|||Manual Length = 1647 Score = 25.8 bits (54), Expect = 5.7 Identities = 9/28 (32%), Positives = 17/28 (60%) Frame = -1 Query: 93 NFQQKFNNRHFFEHSQSMDFLKKKKKTR 10 N + K +N+H S+ + F KK++ T+ Sbjct: 30 NLRSKSDNKHRLNTSEELPFTKKRRATK 57 >SPAC23D3.06c |nup146||nucleoporin Nup146|Schizosaccharomyces pombe|chr 1|||Manual Length = 1325 Score = 25.4 bits (53), Expect = 7.5 Identities = 11/32 (34%), Positives = 17/32 (53%) Frame = +2 Query: 569 TQLLGQFGELRAXXSTTGSASPHYHRRYSRRD 664 + ++ QF L S+T S HY R + R+D Sbjct: 1277 SDMMTQFRALGISQSSTKQVSAHYLREFERQD 1308 >SPAC12G12.14c |pfs2||WD repeat protein Pfs2|Schizosaccharomyces pombe|chr 1|||Manual Length = 509 Score = 25.0 bits (52), Expect = 9.9 Identities = 24/105 (22%), Positives = 39/105 (37%), Gaps = 6/105 (5%) Frame = -1 Query: 315 NHHPLHSPIAL*NH*-HNMGMLILQNIFKFYITPVAEKP*C-----WSSFYHCVSHVKLI 154 NH+ L P L H + + + + P AE P WS YH + H+ Sbjct: 318 NHYSLDEPPLLSQQKYHEKHPNVTLSASSYLLYPTAEIPFAHDLGIWSMQYHPLGHLLCT 377 Query: 153 NTTAPINTYYLNSIPKDCALNFQQKFNNRHFFEHSQSMDFLKKKK 19 + ++ S P D ++ +RH QS L ++K Sbjct: 378 GSNDKTTRFWSRSRPDD-----KESTMDRHHLGEEQSEAMLSQRK 417 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,600,811 Number of Sequences: 5004 Number of extensions: 49688 Number of successful extensions: 123 Number of sequences better than 10.0: 4 Number of HSP's better than 10.0 without gapping: 119 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 123 length of database: 2,362,478 effective HSP length: 70 effective length of database: 2,012,198 effective search space used: 307866294 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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