BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= NV060451.seq (683 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, wit... 29 0.83 SPAC17C9.03 |tif471||translation initiation factor eIF4G |Schizo... 28 1.1 SPBP8B7.30c |thi5||transcription factor Thi5|Schizosaccharomyces... 28 1.4 SPBC1105.07c |||nuclear pore associated protein Thp1-Sac3 comple... 28 1.4 SPBC11B10.08 |||conserved fungal protein|Schizosaccharomyces pom... 28 1.4 SPCC1739.13 |ssa2||heat shock protein Ssa2|Schizosaccharomyces p... 28 1.4 SPAC343.12 |rds1||conserved fungal protein|Schizosaccharomyces p... 27 1.9 SPAC222.10c |byr4||two-component GAP Byr4|Schizosaccharomyces po... 27 1.9 SPAC1D4.11c |lkh1|kic1|dual specificity protein kinase Lkh1|Schi... 27 1.9 SPBC800.09 |sum2||G2/M transition checkpoint protein Sum2|Schizo... 27 2.5 SPBC649.04 |uvi15||UV-induced protein Uvi15|Schizosaccharomyces ... 26 4.4 SPAC18B11.10 |tup11||transcriptional corepressor Tup11|Schizosac... 26 4.4 SPAC3C7.09 |set8||lysine methyltransferase Set8 |Schizosaccharom... 26 4.4 SPBC15C4.01c |oca3||TPR repeat protein Oca3|Schizosaccharomyces ... 26 4.4 SPAC6G9.04 |mug79||meiotically upregulated gene Mug79|Schizosacc... 26 5.8 SPAC19G12.10c |cpy1|pcy1|vacuolar carboxypeptidase Y|Schizosacch... 26 5.8 SPBC3B8.02 |php5||CCAAT-binding factor complex subunit Php5|Schi... 26 5.8 SPAPJ760.02c |app1||App1 protein|Schizosaccharomyces pombe|chr 1... 26 5.8 SPAC31G5.01 |sap49|SPAPB1A11.05|RNA-binding protein Sap49|Schizo... 26 5.8 >SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, with EF hand and WH2 motif |Schizosaccharomyces pombe|chr 1|||Manual Length = 1794 Score = 28.7 bits (61), Expect = 0.83 Identities = 24/64 (37%), Positives = 29/64 (45%), Gaps = 5/64 (7%) Frame = +1 Query: 307 QPLPGMQHGFQPGF--QPGYQPGFAPGYPQPSGYPVPVMQQPGPQAPGGWMNMPQ--GLQ 474 QP+ + G QP + G QP PG QP MQ PQ G MPQ G+Q Sbjct: 633 QPMAPQRTGMQPMMPQRTGMQPQM-PGMQQPMAPQRTGMQPMAPQRTGMQPMMPQRTGMQ 691 Query: 475 -QLP 483 Q+P Sbjct: 692 PQMP 695 Score = 27.5 bits (58), Expect = 1.9 Identities = 21/48 (43%), Positives = 25/48 (52%), Gaps = 9/48 (18%) Frame = +1 Query: 331 GFQPGFQPGYQPGFAP---GY--PQPSGYPVPVMQQPG----PQAPGG 447 GF Q G PGFAP G+ PQP+G+ + QQP PQ GG Sbjct: 110 GFMQPQQTGAIPGFAPQPTGFVQPQPTGF---MSQQPASFMQPQRTGG 154 Score = 25.8 bits (54), Expect = 5.8 Identities = 24/69 (34%), Positives = 31/69 (44%), Gaps = 5/69 (7%) Frame = +1 Query: 307 QPLPGMQHGFQP---GFQPGYQPGFAPGYPQPSGYPVPVMQQPGPQAPGGWMNMPQGLQQ 477 QP+ + G QP GFQ P PQ +G+ P+M PQ G M G QQ Sbjct: 456 QPMMPQRTGLQPQMTGFQQPMVPQRTGMQPQMTGFQQPMM----PQRTGLQPQM-TGFQQ 510 Query: 478 --LPSRFGI 498 +P R G+ Sbjct: 511 PMVPQRTGM 519 Score = 25.8 bits (54), Expect = 5.8 Identities = 15/38 (39%), Positives = 16/38 (42%) Frame = +2 Query: 140 MSHKPTPYSPNFPASHGYVPPPEGEKPNESYPMHPQSG 253 MS P P +P PA PPP P S P P G Sbjct: 1712 MSVPPPPSAPPMPAGPPSAPPP--PLPASSAPSVPNPG 1747 >SPAC17C9.03 |tif471||translation initiation factor eIF4G |Schizosaccharomyces pombe|chr 1|||Manual Length = 1403 Score = 28.3 bits (60), Expect = 1.1 Identities = 18/53 (33%), Positives = 25/53 (47%), Gaps = 3/53 (5%) Frame = +2 Query: 107 IYEKGYETELTMSHKPTPYSPNFP---ASHGYVPPPEGEKPNESYPMHPQSGF 256 I + GY + + TPY+ NFP S V E PN S + P++GF Sbjct: 413 ILQPGYVPQYYPVYHQTPYTQNFPNMSRSGSQVSDQVVESPNSS-TLSPRNGF 464 Score = 25.8 bits (54), Expect = 5.8 Identities = 14/35 (40%), Positives = 17/35 (48%), Gaps = 1/35 (2%) Frame = +1 Query: 334 FQPGFQPGYQPGFAPGYPQ-PSGYPVPVMQQPGPQ 435 + P QPGY P + P Y Q P P M + G Q Sbjct: 410 YGPILQPGYVPQYYPVYHQTPYTQNFPNMSRSGSQ 444 >SPBP8B7.30c |thi5||transcription factor Thi5|Schizosaccharomyces pombe|chr 2|||Manual Length = 857 Score = 27.9 bits (59), Expect = 1.4 Identities = 12/37 (32%), Positives = 13/37 (35%) Frame = +2 Query: 164 SPNFPASHGYVPPPEGEKPNESYPMHPQSGFLAHHPL 274 S N P H P N P HP FL + L Sbjct: 791 SSNIPLGHALGNPESNNSSNSFKPSHPSQSFLVNESL 827 >SPBC1105.07c |||nuclear pore associated protein Thp1-Sac3 complex subunit |Schizosaccharomyces pombe|chr 2|||Manual Length = 442 Score = 27.9 bits (59), Expect = 1.4 Identities = 17/50 (34%), Positives = 22/50 (44%), Gaps = 7/50 (14%) Frame = +3 Query: 138 QCHINQHHTHQIS-------LQVMDMYLHQKEKSQMKVTPCILKAASWPT 266 +CH+ Q HQ LQ D HQK S + +T C+L PT Sbjct: 232 RCHLYQRKIHQAKDHLLFSFLQCPDECYHQKRLSLIYLTTCLLILGKSPT 281 >SPBC11B10.08 |||conserved fungal protein|Schizosaccharomyces pombe|chr 2|||Manual Length = 204 Score = 27.9 bits (59), Expect = 1.4 Identities = 18/48 (37%), Positives = 18/48 (37%) Frame = +1 Query: 304 AQPLPGMQHGFQPGFQPGYQPGFAPGYPQPSGYPVPVMQQPGPQAPGG 447 A P P G P QP Y P YP Y PV QP A G Sbjct: 98 AAPQPYPPQGGYPQ-QPYYYPNQPNYYPAQPAYAQPVYAQPATSARRG 144 >SPCC1739.13 |ssa2||heat shock protein Ssa2|Schizosaccharomyces pombe|chr 3|||Manual Length = 647 Score = 27.9 bits (59), Expect = 1.4 Identities = 12/22 (54%), Positives = 12/22 (54%) Frame = +1 Query: 316 PGMQHGFQPGFQPGYQPGFAPG 381 PG G PG PG PG APG Sbjct: 615 PGGMPGAAPGAAPGAAPGAAPG 636 Score = 25.4 bits (53), Expect = 7.7 Identities = 11/19 (57%), Positives = 11/19 (57%) Frame = +1 Query: 325 QHGFQPGFQPGYQPGFAPG 381 Q G PG PG PG APG Sbjct: 610 QAGGAPGGMPGAAPGAAPG 628 >SPAC343.12 |rds1||conserved fungal protein|Schizosaccharomyces pombe|chr 1|||Manual Length = 402 Score = 27.5 bits (58), Expect = 1.9 Identities = 11/30 (36%), Positives = 15/30 (50%) Frame = +2 Query: 170 NFPASHGYVPPPEGEKPNESYPMHPQSGFL 259 NFPA H PP N S+P +P ++ Sbjct: 248 NFPALHLVSPPVHTNFTNGSFPQYPNGTYM 277 >SPAC222.10c |byr4||two-component GAP Byr4|Schizosaccharomyces pombe|chr 1|||Manual Length = 665 Score = 27.5 bits (58), Expect = 1.9 Identities = 13/37 (35%), Positives = 21/37 (56%) Frame = +3 Query: 189 DMYLHQKEKSQMKVTPCILKAASWPTTHSYATPRPTS 299 ++ L +K+ +++ TP A S HS+ TPR TS Sbjct: 398 ELKLRKKQTTKVSGTPKSKHAGSTQEWHSHTTPRSTS 434 >SPAC1D4.11c |lkh1|kic1|dual specificity protein kinase Lkh1|Schizosaccharomyces pombe|chr 1|||Manual Length = 690 Score = 27.5 bits (58), Expect = 1.9 Identities = 17/46 (36%), Positives = 23/46 (50%), Gaps = 2/46 (4%) Frame = +2 Query: 143 SHKPTPYSPNFPASHGYV--PPPEGEKPNESYPMHPQSGFLAHHPL 274 SH P ++P P S V PPP + P+ YP+ S L H P+ Sbjct: 136 SHHPPLHNP-LPVSCQPVLRPPPVPQVPSHWYPVSLPSPNLPHQPI 180 >SPBC800.09 |sum2||G2/M transition checkpoint protein Sum2|Schizosaccharomyces pombe|chr 2|||Manual Length = 426 Score = 27.1 bits (57), Expect = 2.5 Identities = 20/61 (32%), Positives = 25/61 (40%), Gaps = 2/61 (3%) Frame = +1 Query: 325 QHGFQPGFQPGYQPGFAPGYPQP--SGYPVPVMQQPGPQAPGGWMNMPQGLQQLPSRFGI 498 Q G +P F GFA P GY +P Q+P APG P + PS + Sbjct: 176 QMGQRPEFAQNVSQGFAGQAPYNVRPGYGMPSNQKPPNFAPGMPAPGPTAVSASPSLQSM 235 Query: 499 P 501 P Sbjct: 236 P 236 >SPBC649.04 |uvi15||UV-induced protein Uvi15|Schizosaccharomyces pombe|chr 2|||Manual Length = 87 Score = 26.2 bits (55), Expect = 4.4 Identities = 19/54 (35%), Positives = 21/54 (38%), Gaps = 8/54 (14%) Frame = +1 Query: 310 PLPGMQHGFQPGFQPGYQPGFAPGYPQPSGYP---VPV-----MQQPGPQAPGG 447 P P Q P + P Q GY P GYP P +QQP PGG Sbjct: 14 PAPPQQAYGGPNYYPPQQNYPQQGYAPPQGYPQGGYPAQQPMYVQQPQASDPGG 67 >SPAC18B11.10 |tup11||transcriptional corepressor Tup11|Schizosaccharomyces pombe|chr 1|||Manual Length = 614 Score = 26.2 bits (55), Expect = 4.4 Identities = 14/36 (38%), Positives = 14/36 (38%), Gaps = 1/36 (2%) Frame = +1 Query: 340 PGFQPGYQPGFAPGYPQPSGYPV-PVMQQPGPQAPG 444 P P P Y P Y V QQP PQ PG Sbjct: 180 PAISTSNLPSTTPLYIPPVNYGANQVSQQPNPQLPG 215 >SPAC3C7.09 |set8||lysine methyltransferase Set8 |Schizosaccharomyces pombe|chr 1|||Manual Length = 429 Score = 26.2 bits (55), Expect = 4.4 Identities = 10/29 (34%), Positives = 19/29 (65%) Frame = +2 Query: 131 ELTMSHKPTPYSPNFPASHGYVPPPEGEK 217 E+T+++ S F S+G++P PEG++ Sbjct: 226 EVTINYGSEKGSAEFLFSYGFLPEPEGDR 254 >SPBC15C4.01c |oca3||TPR repeat protein Oca3|Schizosaccharomyces pombe|chr 2|||Manual Length = 282 Score = 26.2 bits (55), Expect = 4.4 Identities = 13/31 (41%), Positives = 17/31 (54%) Frame = -3 Query: 612 FXPTLFNKLCNLLFVSTPTKASNNSTXLMHY 520 F P LF +L +L FV + A+N L HY Sbjct: 171 FEPRLFARLGDLYFVLAQSNATNYWFSLKHY 201 >SPAC6G9.04 |mug79||meiotically upregulated gene Mug79|Schizosaccharomyces pombe|chr 1|||Manual Length = 1318 Score = 25.8 bits (54), Expect = 5.8 Identities = 15/32 (46%), Positives = 16/32 (50%) Frame = +3 Query: 228 VTPCILKAASWPTTHSYATPRPTSWGTTPSGH 323 V P L AA PTT + T PTS P GH Sbjct: 358 VQPQALAAAESPTTKAPTTKAPTS-EAPPKGH 388 >SPAC19G12.10c |cpy1|pcy1|vacuolar carboxypeptidase Y|Schizosaccharomyces pombe|chr 1|||Manual Length = 1002 Score = 25.8 bits (54), Expect = 5.8 Identities = 20/68 (29%), Positives = 24/68 (35%), Gaps = 4/68 (5%) Frame = +2 Query: 122 YETELTMSHKPTPYSPNFPASHG----YVPPPEGEKPNESYPMHPQSGFLAHHPLLCHTP 289 ++ E H+ FPA H PPP KP E P P HH H P Sbjct: 180 FDDERPKHHEFDDEDREFPAHHEPGEHMPPPPMHHKPGEHMPPPPM-----HHEPGEHMP 234 Query: 290 AYILGHNP 313 + H P Sbjct: 235 PPPMHHEP 242 >SPBC3B8.02 |php5||CCAAT-binding factor complex subunit Php5|Schizosaccharomyces pombe|chr 2|||Manual Length = 415 Score = 25.8 bits (54), Expect = 5.8 Identities = 15/52 (28%), Positives = 22/52 (42%), Gaps = 2/52 (3%) Frame = +1 Query: 331 GFQPGFQPGYQPGFAP--GYPQPSGYPVPVMQQPGPQAPGGWMNMPQGLQQL 480 G G QP P G+P P+G +P Q Q + + P +QQ+ Sbjct: 211 GGMNGLQPFPTQAGLPNQGFPMPTGSQLPFSNQQSSQPSMQYSSHPSRMQQM 262 >SPAPJ760.02c |app1||App1 protein|Schizosaccharomyces pombe|chr 1|||Manual Length = 857 Score = 25.8 bits (54), Expect = 5.8 Identities = 12/27 (44%), Positives = 15/27 (55%) Frame = +2 Query: 149 KPTPYSPNFPASHGYVPPPEGEKPNES 229 KPTP+ + PA V P E KP+ S Sbjct: 252 KPTPFESHGPAKQISVQPSEHPKPSIS 278 >SPAC31G5.01 |sap49|SPAPB1A11.05|RNA-binding protein Sap49|Schizosaccharomyces pombe|chr 1|||Manual Length = 335 Score = 25.8 bits (54), Expect = 5.8 Identities = 16/40 (40%), Positives = 23/40 (57%) Frame = +3 Query: 210 EKSQMKVTPCILKAASWPTTHSYATPRPTSWGTTPSGHAA 329 +K+++ VTP ++ P S ATP PTS TP+ AA Sbjct: 198 KKNKVAVTP----QSTLPPGFSPATPAPTSAANTPATIAA 233 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,656,115 Number of Sequences: 5004 Number of extensions: 54105 Number of successful extensions: 194 Number of sequences better than 10.0: 19 Number of HSP's better than 10.0 without gapping: 172 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 192 length of database: 2,362,478 effective HSP length: 70 effective length of database: 2,012,198 effective search space used: 315915086 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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