Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= NV060449.seq
(685 letters)
Database: bee
438 sequences; 146,343 total letters
Searching......................................................done
Score E
Sequences producing significant alignments: (bits) Value
AY208278-1|AAO48970.1| 274|Apis mellifera elongation factor 1-a... 202 3e-54
AF015267-1|AAC38959.1| 461|Apis mellifera elongation factor-1al... 202 3e-54
EF013389-1|ABK54743.1| 172|Apis mellifera elongation factor 1-a... 194 7e-52
X52884-1|CAA37066.1| 461|Apis mellifera elongation factor 1 alp... 194 9e-52
AF069739-1|AAC63272.2| 690|Apis mellifera translation initiatio... 33 0.003
AM050259-1|CAJ18340.1| 683|Apis mellifera putative H3K9 methylt... 25 0.89
AB253417-1|BAE86928.1| 567|Apis mellifera alpha-glucosidase pro... 23 2.7
D79208-1|BAA11466.1| 567|Apis mellifera alpha-glucosidase protein. 23 3.6
DQ232888-1|ABB36783.1| 499|Apis mellifera cytochrome P450 monoo... 23 3.6
DQ071552-1|AAY82248.1| 495|Apis mellifera anarchy 1 protein. 22 4.7
AB204558-1|BAD89803.1| 1143|Apis mellifera nitric oxide synthase... 22 6.3
DQ342041-1|ABC69933.1| 828|Apis mellifera STIP protein. 21 8.3
AF393495-1|AAL60420.1| 136|Apis mellifera odorant binding prote... 21 8.3
AF388659-3|AAK71993.1| 548|Apis mellifera 1D-myo-inositol-trisp... 21 8.3
AF388659-2|AAK71994.1| 463|Apis mellifera 1D-myo-inositol-trisp... 21 8.3
AF388659-1|AAK71995.1| 782|Apis mellifera 1D-myo-inositol-trisp... 21 8.3
AB253416-1|BAE86927.1| 580|Apis mellifera alpha-glucosidase pro... 21 8.3
>AY208278-1|AAO48970.1| 274|Apis mellifera elongation factor
1-alpha protein.
Length = 274
Score = 202 bits (492), Expect = 3e-54
Identities = 99/126 (78%), Positives = 106/126 (84%)
Frame = +2
Query: 257 GITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISK 436
GITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISK
Sbjct: 13 GITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISK 72
Query: 437 NGQTREHALLAFTLGVKQLIVGVNKWIPLNHHTVSPDLRKSRRKYPHTSRRLGYNPAAVA 616
NGQTREHALLAFTLGVKQLIVGVNK + +++ +++GYNPAAVA
Sbjct: 73 NGQTREHALLAFTLGVKQLIVGVNKMDSTEPPYSETRFEEIKKEVSSYIKKIGYNPAAVA 132
Query: 617 FVPISG 634
FVPISG
Sbjct: 133 FVPISG 138
Score = 28.3 bits (60), Expect = 0.072
Identities = 11/12 (91%), Positives = 12/12 (100%)
Frame = +1
Query: 223 WVLDKLKAERDR 258
WVLDKLKAER+R
Sbjct: 1 WVLDKLKAERER 12
Score = 27.1 bits (57), Expect = 0.17
Identities = 9/11 (81%), Positives = 9/11 (81%)
Frame = +3
Query: 624 PFLGWHGDNML 656
P GWHGDNML
Sbjct: 135 PISGWHGDNML 145
>AF015267-1|AAC38959.1| 461|Apis mellifera elongation factor-1alpha
F2 protein.
Length = 461
Score = 202 bits (492), Expect = 3e-54
Identities = 99/126 (78%), Positives = 106/126 (84%)
Frame = +2
Query: 257 GITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISK 436
GITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISK
Sbjct: 70 GITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISK 129
Query: 437 NGQTREHALLAFTLGVKQLIVGVNKWIPLNHHTVSPDLRKSRRKYPHTSRRLGYNPAAVA 616
NGQTREHALLAFTLGVKQLIVGVNK + +++ +++GYNPAAVA
Sbjct: 130 NGQTREHALLAFTLGVKQLIVGVNKMDSTEPPYSETRFEEIKKEVSSYIKKIGYNPAAVA 189
Query: 617 FVPISG 634
FVPISG
Sbjct: 190 FVPISG 195
Score = 146 bits (354), Expect = 2e-37
Identities = 67/69 (97%), Positives = 68/69 (98%)
Frame = +1
Query: 52 MGKEKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVL 231
MGKEK HINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVL
Sbjct: 1 MGKEKIHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVL 60
Query: 232 DKLKAERDR 258
DKLKAER+R
Sbjct: 61 DKLKAERER 69
Score = 27.1 bits (57), Expect = 0.17
Identities = 9/11 (81%), Positives = 9/11 (81%)
Frame = +3
Query: 624 PFLGWHGDNML 656
P GWHGDNML
Sbjct: 192 PISGWHGDNML 202
>EF013389-1|ABK54743.1| 172|Apis mellifera elongation factor
1-alpha protein.
Length = 172
Score = 194 bits (473), Expect = 7e-52
Identities = 95/122 (77%), Positives = 102/122 (83%)
Frame = +2
Query: 269 DIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNGQT 448
DIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNGQT
Sbjct: 1 DIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNGQT 60
Query: 449 REHALLAFTLGVKQLIVGVNKWIPLNHHTVSPDLRKSRRKYPHTSRRLGYNPAAVAFVPI 628
REHALLAFTLGVKQLIVGVNK + +++ +++GYNPAAVAFVPI
Sbjct: 61 REHALLAFTLGVKQLIVGVNKMDSTEPPYSETRFEEIKKEVSSYIKKIGYNPAAVAFVPI 120
Query: 629 SG 634
SG
Sbjct: 121 SG 122
Score = 27.1 bits (57), Expect = 0.17
Identities = 9/11 (81%), Positives = 9/11 (81%)
Frame = +3
Query: 624 PFLGWHGDNML 656
P GWHGDNML
Sbjct: 119 PISGWHGDNML 129
>X52884-1|CAA37066.1| 461|Apis mellifera elongation factor 1 alpha
protein.
Length = 461
Score = 194 bits (472), Expect = 9e-52
Identities = 95/126 (75%), Positives = 105/126 (83%)
Frame = +2
Query: 257 GITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISK 436
GITIDIALWKFET+KYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAG GEFEAGISK
Sbjct: 70 GITIDIALWKFETAKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGIGEFEAGISK 129
Query: 437 NGQTREHALLAFTLGVKQLIVGVNKWIPLNHHTVSPDLRKSRRKYPHTSRRLGYNPAAVA 616
NGQTREHALLAFTLGVKQLIVGVNK + + +++ +++GYN A+VA
Sbjct: 130 NGQTREHALLAFTLGVKQLIVGVNKMDMTDPPYSEARFEEIKKEVSSYIKKIGYNTASVA 189
Query: 617 FVPISG 634
FVPISG
Sbjct: 190 FVPISG 195
Score = 146 bits (354), Expect = 2e-37
Identities = 67/69 (97%), Positives = 68/69 (98%)
Frame = +1
Query: 52 MGKEKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVL 231
MGKEK HINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVL
Sbjct: 1 MGKEKIHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVL 60
Query: 232 DKLKAERDR 258
DKLKAER+R
Sbjct: 61 DKLKAERER 69
Score = 27.1 bits (57), Expect = 0.17
Identities = 9/11 (81%), Positives = 9/11 (81%)
Frame = +3
Query: 624 PFLGWHGDNML 656
P GWHGDNML
Sbjct: 192 PISGWHGDNML 202
>AF069739-1|AAC63272.2| 690|Apis mellifera translation initiation
factor 2 protein.
Length = 690
Score = 33.1 bits (72), Expect = 0.003
Identities = 16/35 (45%), Positives = 18/35 (51%)
Frame = +2
Query: 308 VTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTG 412
VT +D PGH FI G D VL+VAA G
Sbjct: 195 VTFLDTPGHAAFISMRHRGAHITDIVVLVVAADDG 229
Score = 25.4 bits (53), Expect = 0.51
Identities = 9/18 (50%), Positives = 13/18 (72%)
Frame = +1
Query: 64 KTHINIVVIGHVDSGKST 117
K H + ++GHVD GK+T
Sbjct: 143 KRHPIVTIMGHVDHGKTT 160
>AM050259-1|CAJ18340.1| 683|Apis mellifera putative H3K9
methyltransferase protein.
Length = 683
Score = 24.6 bits (51), Expect = 0.89
Identities = 12/23 (52%), Positives = 14/23 (60%)
Frame = +1
Query: 49 EMGKEKTHINIVVIGHVDSGKST 117
E+ + INI IGHV GKST
Sbjct: 35 EVISRQATINIGTIGHVAHGKST 57
>AB253417-1|BAE86928.1| 567|Apis mellifera alpha-glucosidase
protein.
Length = 567
Score = 23.0 bits (47), Expect = 2.7
Identities = 13/34 (38%), Positives = 18/34 (52%)
Frame = +2
Query: 293 TSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLI 394
T KYY D P + FIKN+ ++ +D LI
Sbjct: 294 TMKYYDYGADFPFNFAFIKNVSRDSNSSDFKKLI 327
>D79208-1|BAA11466.1| 567|Apis mellifera alpha-glucosidase protein.
Length = 567
Score = 22.6 bits (46), Expect = 3.6
Identities = 12/34 (35%), Positives = 18/34 (52%)
Frame = +2
Query: 293 TSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLI 394
T KYY D P + FIKN+ ++ +D L+
Sbjct: 294 TMKYYDYGADFPFNFAFIKNVSRDSNSSDFKKLV 327
>DQ232888-1|ABB36783.1| 499|Apis mellifera cytochrome P450
monooxygenase protein.
Length = 499
Score = 22.6 bits (46), Expect = 3.6
Identities = 11/29 (37%), Positives = 16/29 (55%)
Frame = -3
Query: 260 YRSRSAFSLSNTQAYLKDPLPISWASFSN 174
Y +RS F L N +KD L ++ F+N
Sbjct: 76 YATRSPFLLLNDPELIKDILIRDFSKFAN 104
>DQ071552-1|AAY82248.1| 495|Apis mellifera anarchy 1 protein.
Length = 495
Score = 22.2 bits (45), Expect = 4.7
Identities = 11/36 (30%), Positives = 19/36 (52%)
Frame = -1
Query: 307 IVLASFELPESNIDCDTDHAQPLVCPIPKHI*RILY 200
IV+ FE+ +S D + A IP ++ R+L+
Sbjct: 54 IVIGGFEIEKSEDDSFNNQADKSEKRIPLYVCRVLH 89
Score = 21.4 bits (43), Expect = 8.3
Identities = 24/107 (22%), Positives = 46/107 (42%), Gaps = 3/107 (2%)
Frame = -1
Query: 532 MVVQWNPFVYSYDELFDTEGESEQGMLTGLTVLRDTSFEFTGTGSYDEHSAISLRGSCDH 353
M V ++ + S+ E +T+ G+ + T+ D EF +S + +
Sbjct: 329 MEVPYSGTLISHYEDGETKSRFISGIRSEQTIF-DVKPEFGSIYFLGNYSLVPTTTASPT 387
Query: 352 VLDEISVSRSINDGNI---VLASFELPESNIDCDTDHAQPLVCPIPK 221
+ S +I+ +I V+ L E N+D + DH + ++ P PK
Sbjct: 388 TEPSTTTSTTISQKHIKVFVVNKDILHEHNVDDNEDHDENMIIP-PK 433
>AB204558-1|BAD89803.1| 1143|Apis mellifera nitric oxide synthase
protein.
Length = 1143
Score = 21.8 bits (44), Expect = 6.3
Identities = 10/32 (31%), Positives = 16/32 (50%)
Frame = -3
Query: 632 QKWARKRQQLGCSPIFLMYEDTSFLISSNLGS 537
+KWA + C L +DT ++ +LGS
Sbjct: 655 RKWAADTFAVACETFCLDDDDTLLEVALSLGS 686
>DQ342041-1|ABC69933.1| 828|Apis mellifera STIP protein.
Length = 828
Score = 21.4 bits (43), Expect = 8.3
Identities = 9/28 (32%), Positives = 16/28 (57%)
Frame = +1
Query: 487 TAHRRSKQMDSTEPPYSEPRFEEIKKEV 570
T+ R++ S PP ++PR E + + V
Sbjct: 725 TSLERTQPTMSQMPPTAQPRMERLAEAV 752
>AF393495-1|AAL60420.1| 136|Apis mellifera odorant binding protein
ASP4 protein.
Length = 136
Score = 21.4 bits (43), Expect = 8.3
Identities = 8/20 (40%), Positives = 12/20 (60%)
Frame = +1
Query: 580 IKKIGLQPSCCRFRAHFWDG 639
+KK+G +C + HF DG
Sbjct: 49 MKKLGCFEACVFQKLHFMDG 68
>AF388659-3|AAK71993.1| 548|Apis mellifera
1D-myo-inositol-trisphosphate 3-kinaseisoform C protein.
Length = 548
Score = 21.4 bits (43), Expect = 8.3
Identities = 12/32 (37%), Positives = 14/32 (43%)
Frame = -3
Query: 668 VEGSQHVVSVPSQKWARKRQQLGCSPIFLMYE 573
VEG H V Q+ R L SP F +E
Sbjct: 447 VEGYPHAVPKYIQRLKAIRATLKASPFFASHE 478
>AF388659-2|AAK71994.1| 463|Apis mellifera
1D-myo-inositol-trisphosphate 3-kinaseisoform B protein.
Length = 463
Score = 21.4 bits (43), Expect = 8.3
Identities = 12/32 (37%), Positives = 14/32 (43%)
Frame = -3
Query: 668 VEGSQHVVSVPSQKWARKRQQLGCSPIFLMYE 573
VEG H V Q+ R L SP F +E
Sbjct: 362 VEGYPHAVPKYIQRLKAIRATLKASPFFASHE 393
>AF388659-1|AAK71995.1| 782|Apis mellifera
1D-myo-inositol-trisphosphate 3-kinaseisoform A protein.
Length = 782
Score = 21.4 bits (43), Expect = 8.3
Identities = 12/32 (37%), Positives = 14/32 (43%)
Frame = -3
Query: 668 VEGSQHVVSVPSQKWARKRQQLGCSPIFLMYE 573
VEG H V Q+ R L SP F +E
Sbjct: 681 VEGYPHAVPKYIQRLKAIRATLKASPFFASHE 712
>AB253416-1|BAE86927.1| 580|Apis mellifera alpha-glucosidase
protein.
Length = 580
Score = 21.4 bits (43), Expect = 8.3
Identities = 7/20 (35%), Positives = 13/20 (65%)
Frame = +2
Query: 512 WIPLNHHTVSPDLRKSRRKY 571
W+P+N + S +L +R+Y
Sbjct: 443 WLPVNENYKSLNLAAQKREY 462
Database: bee
Posted date: Oct 23, 2007 1:17 PM
Number of letters in database: 146,343
Number of sequences in database: 438
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 199,711
Number of Sequences: 438
Number of extensions: 4349
Number of successful extensions: 44
Number of sequences better than 10.0: 17
Number of HSP's better than 10.0 without gapping: 27
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 36
length of database: 146,343
effective HSP length: 56
effective length of database: 121,815
effective search space used: 20830365
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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