Bombyx BLAST Result against spombe

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= NV060440.seq
         (681 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPCP31B10.07 |eft202||translation elongation factor 2 |Schizosac...   162   5e-41
SPAC513.01c |eft201|eft2-1, etf2, SPAPYUK71.04c|translation elon...   162   5e-41
SPBC215.12 |cwf10|spef2, snu114|GTPase Cwf10 |Schizosaccharomyce...    84   2e-17
SPCC553.08c |||GTPase Ria1 |Schizosaccharomyces pombe|chr 3|||Ma...    82   6e-17
SPBC1306.01c ||SPBC409.22c|translation elongation factor G|Schiz...    43   5e-05
SPBC660.10 |||translation elongation factor G|Schizosaccharomyce...    38   0.001
SPAC22H12.02 |tfg3|taf14|transcription factor TFIIF complex subu...    29   0.62 
SPCC4B3.17 |cbp3||ubiquinol cytochrome-c reductase assembly prot...    27   2.5  
SPCC737.08 |||midasin |Schizosaccharomyces pombe|chr 3|||Manual        27   3.3  
SPAC24H6.13 |||DUF221 family protein|Schizosaccharomyces pombe|c...    26   4.4  
SPCC622.12c |||NADP-specific glutamate dehydrogenase |Schizosacc...    26   5.8  
SPAC12G12.12 |||NST UDP-galactose transporter|Schizosaccharomyce...    26   5.8  
SPBC1347.01c |rev1|SPBC215.16c|deoxycytidyl transferase Rev1 |Sc...    26   5.8  
SPBC3F6.05 |rga1||GTPase activating protein Rga1|Schizosaccharom...    26   5.8  
SPAC23E2.03c |ste7||meiotic suppressor protein Ste7|Schizosaccha...    25   7.7  

>SPCP31B10.07 |eft202||translation elongation factor 2
           |Schizosaccharomyces pombe|chr 3|||Manual
          Length = 842

 Score =  162 bits (393), Expect = 5e-41
 Identities = 85/136 (62%), Positives = 101/136 (74%), Gaps = 1/136 (0%)
 Frame = +2

Query: 257 LVKTGTITTFKNAHNMKVMKFSVSPVVRVAVEPKNPADLPKLVEGLKRLAKSDPMVQCIN 436
           LVK+GT+TT + AHNMKVMKFSVSPVV+VAVE KN  DLPKLVEGLKRL+KSDP V C  
Sbjct: 463 LVKSGTLTTSEVAHNMKVMKFSVSPVVQVAVEVKNGNDLPKLVEGLKRLSKSDPCVLCTT 522

Query: 437 EESGEHIVAGAGELHLEICLKDLEXTMLAFQSRSLTLSCRTVRP*LRNRNQL-CLSKSPN 613
            ESGEHIVAGAGELHLEICLKDL+        + ++    + R  +   + +  LSKSPN
Sbjct: 523 SESGEHIVAGAGELHLEICLKDLQEDHAGIPLK-ISPPVVSYRESVSEPSSMTALSKSPN 581

Query: 614 KHNRLFMKAQPMPDGL 661
           KHNR+FM A+PM + L
Sbjct: 582 KHNRIFMTAEPMSEEL 597



 Score =  132 bits (320), Expect = 3e-32
 Identities = 60/82 (73%), Positives = 69/82 (84%)
 Frame = +3

Query: 9   VYVSKMVPTSDKGRFYAFGRVFSGKVVTGQKARIMGPNFTPGKKEDLYEKTIQRTILMMG 188
           +YVSKMVPTSD+GRFYAFGRVFSG V +G K RI GPN+ PGKK+DL+ K IQRT+LMMG
Sbjct: 380 IYVSKMVPTSDRGRFYAFGRVFSGTVRSGLKVRIQGPNYVPGKKDDLFIKAIQRTVLMMG 439

Query: 189 RYVEAIEDVPSGNICGLVGVDQ 254
             +E IED P+GNI GLVGVDQ
Sbjct: 440 SRIEPIEDCPAGNIIGLVGVDQ 461


>SPAC513.01c |eft201|eft2-1, etf2, SPAPYUK71.04c|translation
           elongation factor 2 |Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 842

 Score =  162 bits (393), Expect = 5e-41
 Identities = 85/136 (62%), Positives = 101/136 (74%), Gaps = 1/136 (0%)
 Frame = +2

Query: 257 LVKTGTITTFKNAHNMKVMKFSVSPVVRVAVEPKNPADLPKLVEGLKRLAKSDPMVQCIN 436
           LVK+GT+TT + AHNMKVMKFSVSPVV+VAVE KN  DLPKLVEGLKRL+KSDP V C  
Sbjct: 463 LVKSGTLTTSEVAHNMKVMKFSVSPVVQVAVEVKNGNDLPKLVEGLKRLSKSDPCVLCTT 522

Query: 437 EESGEHIVAGAGELHLEICLKDLEXTMLAFQSRSLTLSCRTVRP*LRNRNQL-CLSKSPN 613
            ESGEHIVAGAGELHLEICLKDL+        + ++    + R  +   + +  LSKSPN
Sbjct: 523 SESGEHIVAGAGELHLEICLKDLQEDHAGIPLK-ISPPVVSYRESVSEPSSMTALSKSPN 581

Query: 614 KHNRLFMKAQPMPDGL 661
           KHNR+FM A+PM + L
Sbjct: 582 KHNRIFMTAEPMSEEL 597



 Score =  132 bits (320), Expect = 3e-32
 Identities = 60/82 (73%), Positives = 69/82 (84%)
 Frame = +3

Query: 9   VYVSKMVPTSDKGRFYAFGRVFSGKVVTGQKARIMGPNFTPGKKEDLYEKTIQRTILMMG 188
           +YVSKMVPTSD+GRFYAFGRVFSG V +G K RI GPN+ PGKK+DL+ K IQRT+LMMG
Sbjct: 380 IYVSKMVPTSDRGRFYAFGRVFSGTVRSGLKVRIQGPNYVPGKKDDLFIKAIQRTVLMMG 439

Query: 189 RYVEAIEDVPSGNICGLVGVDQ 254
             +E IED P+GNI GLVGVDQ
Sbjct: 440 SRIEPIEDCPAGNIIGLVGVDQ 461


>SPBC215.12 |cwf10|spef2, snu114|GTPase Cwf10 |Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 983

 Score = 84.2 bits (199), Expect = 2e-17
 Identities = 43/114 (37%), Positives = 64/114 (56%)
 Frame = +2

Query: 332 VVRVAVEPKNPADLPKLVEGLKRLAKSDPMVQCINEESGEHIVAGAGELHLEICLKDLEX 511
           V +VAVEP NP++LPKL++GL++  KS P+     EESGEH + G GE++++  L DL  
Sbjct: 600 VFKVAVEPHNPSELPKLLDGLRKTNKSYPLSITKVEESGEHTIFGTGEMYMDCLLYDLRT 659

Query: 512 TMLAFQSRSLTLSCRTVRP*LRNRNQLCLSKSPNKHNRLFMKAQPMPDGLPEDI 673
                + R      R     +   +  C S +PNK NR+ M  +P+  G+  DI
Sbjct: 660 LYSEIEIRVSDPVARFCETAVDTSSIKCFSDTPNKKNRITMVVEPLEKGISNDI 713



 Score = 56.8 bits (131), Expect = 3e-09
 Identities = 28/81 (34%), Positives = 46/81 (56%)
 Frame = +3

Query: 9   VYVSKMVPTSDKGRFYAFGRVFSGKVVTGQKARIMGPNFTPGKKEDLYEKTIQRTILMMG 188
           ++V+K+  T D   FYAF RV+SG+V  GQK +++G N++   +ED+    I    +   
Sbjct: 489 MHVTKLYNTVDANNFYAFARVYSGQVKKGQKVKVLGENYSLEDEEDMVVAHIAEICVPCA 548

Query: 189 RYVEAIEDVPSGNICGLVGVD 251
           RY   ++   +G +  L GVD
Sbjct: 549 RYRLHVDGAVAGMLVLLGGVD 569


>SPCC553.08c |||GTPase Ria1 |Schizosaccharomyces pombe|chr
           3|||Manual
          Length = 1000

 Score = 82.2 bits (194), Expect = 6e-17
 Identities = 38/83 (45%), Positives = 56/83 (67%)
 Frame = +2

Query: 257 LVKTGTITTFKNAHNMKVMKFSVSPVVRVAVEPKNPADLPKLVEGLKRLAKSDPMVQCIN 436
           +++T T+ +  N  N+  +   + P+VRVA+EP  P ++ KLV GL  L ++DP VQ   
Sbjct: 538 VLRTATLCSSPNGPNLVGVTQQMEPIVRVALEPVRPFEMNKLVTGLDMLNQADPCVQIAV 597

Query: 437 EESGEHIVAGAGELHLEICLKDL 505
           EE+GEH++  AGE+HLE CLKDL
Sbjct: 598 EENGEHVIMCAGEIHLERCLKDL 620



 Score = 50.8 bits (116), Expect = 2e-07
 Identities = 24/71 (33%), Positives = 39/71 (54%), Gaps = 1/71 (1%)
 Frame = +3

Query: 39  DKGRFYAFGRVFSGKVVTGQKARIMGPNFTPGKKEDLYEK-TIQRTILMMGRYVEAIEDV 215
           DK     F R++SG +  GQ+  + GP + P   E    K T++   LMMG+ +  +E V
Sbjct: 464 DKDILIGFARIYSGTISVGQEVYVYGPKYDPVNPEKHITKVTVESLYLMMGQELVYLETV 523

Query: 216 PSGNICGLVGV 248
           P+GN+  + G+
Sbjct: 524 PAGNVFAIGGL 534


>SPBC1306.01c ||SPBC409.22c|translation elongation factor
           G|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 770

 Score = 42.7 bits (96), Expect = 5e-05
 Identities = 25/74 (33%), Positives = 41/74 (55%), Gaps = 1/74 (1%)
 Frame = +2

Query: 272 TITTFKNAHNMKVMKFSVSPVVRVAVEPKNPADLPKLVEGLKRLAKSDPMVQC-INEESG 448
           T T    ++ M  M F   PV+ ++++PK+  D     + L R  + DP  +  ++ ES 
Sbjct: 456 TFTDGSVSYTMTSM-FVPEPVISLSLKPKSK-DTTSFSKALNRFQREDPTFRVQLDNESK 513

Query: 449 EHIVAGAGELHLEI 490
           E I++G GELHLE+
Sbjct: 514 ETIISGMGELHLEV 527


>SPBC660.10 |||translation elongation factor G|Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 813

 Score = 38.3 bits (85), Expect = 0.001
 Identities = 18/60 (30%), Positives = 34/60 (56%), Gaps = 1/60 (1%)
 Frame = +2

Query: 329 PVVRVAVEPKNPADLPKLVEGLKRLAKSDPMVQCINE-ESGEHIVAGAGELHLEICLKDL 505
           PV   ++EP +  D P L+E L  + + DP  +   + E+G+ ++ G G +HL++  + L
Sbjct: 443 PVCIASIEPYSLKDEPALLEALANMNREDPSFRYTQDLENGQLLIQGMGIMHLQVSYERL 502


>SPAC22H12.02 |tfg3|taf14|transcription factor TFIIF complex subunit
           Tfg3|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 241

 Score = 29.1 bits (62), Expect = 0.62
 Identities = 16/49 (32%), Positives = 26/49 (53%), Gaps = 6/49 (12%)
 Frame = +2

Query: 362 PADLPKLVEGLKRLAKSD--PMVQCINEESGEHIVA----GAGELHLEI 490
           P D+ KL EGL++L + D   +VQ +NE     +        GE H+++
Sbjct: 172 PVDMDKLAEGLQKLQEDDLLQVVQMVNENKTPDMYVRNDIEGGEFHIDL 220


>SPCC4B3.17 |cbp3||ubiquinol cytochrome-c reductase assembly protein
           Cbp3|Schizosaccharomyces pombe|chr 3|||Manual
          Length = 283

 Score = 27.1 bits (57), Expect = 2.5
 Identities = 12/33 (36%), Positives = 18/33 (54%)
 Frame = -1

Query: 594 QSWFRFLSYGLTVRHDRVRLLDWNASMVXSRSL 496
           QSWF+     L + H R+R L  N  +  S++L
Sbjct: 137 QSWFQITQLHLWILHTRIRGLPKNEKVAFSQAL 169


>SPCC737.08 |||midasin |Schizosaccharomyces pombe|chr 3|||Manual
          Length = 4717

 Score = 26.6 bits (56), Expect = 3.3
 Identities = 19/68 (27%), Positives = 32/68 (47%)
 Frame = +3

Query: 9    VYVSKMVPTSDKGRFYAFGRVFSGKVVTGQKARIMGPNFTPGKKEDLYEKTIQRTILMMG 188
            +YV   VP  D  RFY + ++    + T  +   +  +F P   +D  EK  +   L  G
Sbjct: 2356 IYVWTNVPDCDISRFYCYTKMLGEWMFTLTEKTKLLESFLP---KDSLEKFSELQNLSTG 2412

Query: 189  RYVEAIED 212
             +++AI D
Sbjct: 2413 LHMQAIWD 2420


>SPAC24H6.13 |||DUF221 family protein|Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 871

 Score = 26.2 bits (55), Expect = 4.4
 Identities = 10/22 (45%), Positives = 15/22 (68%)
 Frame = +2

Query: 596 LSKSPNKHNRLFMKAQPMPDGL 661
           ++KS  KHN+L  K +P+P  L
Sbjct: 243 INKSVKKHNKLVKKHKPLPSTL 264


>SPCC622.12c |||NADP-specific glutamate dehydrogenase
           |Schizosaccharomyces pombe|chr 3|||Manual
          Length = 451

 Score = 25.8 bits (54), Expect = 5.8
 Identities = 15/45 (33%), Positives = 25/45 (55%), Gaps = 1/45 (2%)
 Frame = +3

Query: 114 GPNFTP-GKKEDLYEKTIQRTILMMGRYVEAIEDVPSGNICGLVG 245
           G +F P GK ++   +  Q  +  + RY+    DVP+G+I G+ G
Sbjct: 115 GSDFDPKGKSDNEIRRFSQAFMRQLFRYIGPQTDVPAGDI-GVTG 158


>SPAC12G12.12 |||NST UDP-galactose transporter|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 324

 Score = 25.8 bits (54), Expect = 5.8
 Identities = 10/19 (52%), Positives = 15/19 (78%)
 Frame = +1

Query: 556 YRETVAEESEPALSLKVAQ 612
           Y++++AEESEP  S+ V Q
Sbjct: 20  YQDSIAEESEPIKSMAVLQ 38


>SPBC1347.01c |rev1|SPBC215.16c|deoxycytidyl transferase Rev1
           |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 935

 Score = 25.8 bits (54), Expect = 5.8
 Identities = 12/35 (34%), Positives = 19/35 (54%)
 Frame = +1

Query: 520 CIPIKKSDPVVSYRETVAEESEPALSLKVAQQAQP 624
           CI  KK  P ++YR   A  ++  LS   ++ +QP
Sbjct: 133 CIKQKKILPWINYRTFQASSAQATLSFVASKPSQP 167


>SPBC3F6.05 |rga1||GTPase activating protein
           Rga1|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 1150

 Score = 25.8 bits (54), Expect = 5.8
 Identities = 15/32 (46%), Positives = 21/32 (65%)
 Frame = -1

Query: 291 FLKVVMVPVLTKTDRLQQDHRCYQRAHPQ*LQ 196
           +LK+++   LT   RL+QDH C + A PQ LQ
Sbjct: 423 YLKLLIRISLTGGLRLEQDHAC-KHALPQFLQ 453


>SPAC23E2.03c |ste7||meiotic suppressor protein
           Ste7|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 569

 Score = 25.4 bits (53), Expect = 7.7
 Identities = 10/33 (30%), Positives = 22/33 (66%), Gaps = 2/33 (6%)
 Frame = -1

Query: 477 SSPAPATMCSPDSSL--IHCTMGSDLARRLRPS 385
           S+ +P+T+CSPD+++  ++   G  L  ++ P+
Sbjct: 403 SNVSPSTICSPDNNVTFVNLAPGEKLTFKIEPN 435


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,909,887
Number of Sequences: 5004
Number of extensions: 59734
Number of successful extensions: 216
Number of sequences better than 10.0: 15
Number of HSP's better than 10.0 without gapping: 196
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 208
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 313902888
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)