BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= NV060440.seq (681 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At1g56070.1 68414.m06438 elongation factor 2, putative / EF-2, p... 155 3e-38 At5g25230.1 68418.m02991 elongation factor Tu family protein tra... 104 6e-23 At1g06220.2 68414.m00656 elongation factor Tu family protein sim... 100 8e-22 At1g06220.1 68414.m00655 elongation factor Tu family protein sim... 100 8e-22 At3g22980.1 68416.m02898 elongation factor Tu family protein sim... 87 1e-17 At1g62750.1 68414.m07082 elongation factor Tu family protein sim... 58 7e-09 At2g45030.1 68415.m05606 mitochondrial elongation factor, putati... 42 3e-04 At1g45332.1 68414.m05195 mitochondrial elongation factor, putati... 42 3e-04 At2g47500.1 68415.m05929 kinesin motor protein-related 30 1.2 At1g04650.1 68414.m00462 hypothetical protein 29 2.8 At1g24050.1 68414.m03036 expressed protein 28 5.0 At5g26210.1 68418.m03119 PHD finger family protein contains Pfam... 28 6.6 At4g33440.1 68417.m04751 glycoside hydrolase family 28 protein /... 28 6.6 At3g03405.1 68416.m00338 hypothetical protein temporary automate... 28 6.6 At2g41620.1 68415.m05143 nucleoporin interacting component famil... 28 6.6 At1g10750.1 68414.m01229 expressed protein similar to gi 3128199... 28 6.6 At4g21880.1 68417.m03164 pentatricopeptide (PPR) repeat-containi... 27 8.7 At3g47480.1 68416.m05163 calcium-binding EF hand family protein ... 27 8.7 At3g06850.2 68416.m00813 branched chain alpha-keto acid dehydrog... 27 8.7 At3g06850.1 68416.m00812 branched chain alpha-keto acid dehydrog... 27 8.7 At1g51370.2 68414.m05778 F-box family protein contains F-box dom... 27 8.7 At1g51370.1 68414.m05779 F-box family protein contains F-box dom... 27 8.7 >At1g56070.1 68414.m06438 elongation factor 2, putative / EF-2, putative similar to ELONGATION FACTOR 2 GB:O14460 from [Schizosaccharomyces pombe] Length = 843 Score = 155 bits (376), Expect = 3e-38 Identities = 83/144 (57%), Positives = 104/144 (72%), Gaps = 3/144 (2%) Frame = +2 Query: 257 LVKTGTITTFK--NAHNMKVMKFSVSPVVRVAVEPKNPADLPKLVEGLKRLAKSDPMVQC 430 + K T+T K +AH ++ MKFSVSPVVRVAV+ K +DLPKLVEGLKRLAKSDPMV C Sbjct: 461 ITKNATLTNEKEVDAHPIRAMKFSVSPVVRVAVQCKVASDLPKLVEGLKRLAKSDPMVVC 520 Query: 431 INEESGEHIVAGAGELHLEICLKDLEXTMLAFQSRSLTLSCRTVRP*LRNRN-QLCLSKS 607 EESGEHIVAGAGELHLEICLKDL+ + + + R + +R+ + +SKS Sbjct: 521 TMEESGEHIVAGAGELHLEICLKDLQDDFMGGAEIIKSDPVVSFRETVCDRSTRTVMSKS 580 Query: 608 PNKHNRLFMKAQPMPDGLPEDIXE 679 PNKHNRL+M+A+PM +GL E I + Sbjct: 581 PNKHNRLYMEARPMEEGLAEAIDD 604 Score = 126 bits (303), Expect = 2e-29 Identities = 53/82 (64%), Positives = 68/82 (82%) Frame = +3 Query: 9 VYVSKMVPTSDKGRFYAFGRVFSGKVVTGQKARIMGPNFTPGKKEDLYEKTIQRTILMMG 188 +YVSKM+P SDKGRF+AFGRVF+GKV TG K RIMGPN+ PG+K+DLY K++QRT++ MG Sbjct: 378 LYVSKMIPASDKGRFFAFGRVFAGKVSTGMKVRIMGPNYIPGEKKDLYTKSVQRTVIWMG 437 Query: 189 RYVEAIEDVPSGNICGLVGVDQ 254 + E +EDVP GN +VG+DQ Sbjct: 438 KRQETVEDVPCGNTVAMVGLDQ 459 >At5g25230.1 68418.m02991 elongation factor Tu family protein translation Elongation Factor 2, Schizosaccharomyces pombe, PIR:T39902 Length = 973 Score = 104 bits (249), Expect = 6e-23 Identities = 55/145 (37%), Positives = 88/145 (60%), Gaps = 6/145 (4%) Frame = +2 Query: 257 LVKTGTITTF---KNAHNMKVMKFSVSPVVRVAVEPKNPADLPKLVEGLKRLAKSDPMVQ 427 ++KT T+ ++ + + +KF+ PVV+ A EP NP++LPK+VEGL++++KS P+ Sbjct: 559 IMKTATLCNASYDEDVYIFRALKFNTLPVVKTATEPLNPSELPKMVEGLRKISKSYPLAI 618 Query: 428 CINEESGEHIVAGAGELHLEICLKDLEXTMLAFQSR---SLTLSCRTVRP*LRNRNQLCL 598 EESGEH + G GEL+L+ +KDL Q + + C TV + + + C Sbjct: 619 TKVEESGEHTILGTGELYLDSIIKDLRELYSEVQVKVADPVVSFCETV---VESSSMKCF 675 Query: 599 SKSPNKHNRLFMKAQPMPDGLPEDI 673 +++PNK N+L M A+P+ GL EDI Sbjct: 676 AETPNKKNKLTMIAEPLDRGLAEDI 700 Score = 62.5 bits (145), Expect = 2e-10 Identities = 29/81 (35%), Positives = 46/81 (56%) Frame = +3 Query: 9 VYVSKMVPTSDKGRFYAFGRVFSGKVVTGQKARIMGPNFTPGKKEDLYEKTIQRTILMMG 188 V V+K+ P SD F FGRV+SG++ TGQ R++G ++P +ED+ K + + + Sbjct: 476 VNVTKLYPKSDTSVFDVFGRVYSGRLQTGQSVRVLGEGYSPEDEEDMTIKEVTKLWIYQA 535 Query: 189 RYVEAIEDVPSGNICGLVGVD 251 RY + P G+ + GVD Sbjct: 536 RYRIPVSSAPPGSWVLIEGVD 556 >At1g06220.2 68414.m00656 elongation factor Tu family protein similar to Cryptosporidium parvum elongation factor-2 GB:U21667 GI:706974 from [Cryptosporidium parvum] Length = 987 Score = 100 bits (240), Expect = 8e-22 Identities = 52/145 (35%), Positives = 88/145 (60%), Gaps = 6/145 (4%) Frame = +2 Query: 257 LVKTGTITTF---KNAHNMKVMKFSVSPVVRVAVEPKNPADLPKLVEGLKRLAKSDPMVQ 427 ++KT T+ ++ + + ++F+ PVV+ A EP NP++LPK+VEGL++++KS P+ Sbjct: 573 IMKTATLCNASYDEDVYIFRALQFNTLPVVKTATEPLNPSELPKMVEGLRKISKSYPLAI 632 Query: 428 CINEESGEHIVAGAGELHLEICLKDLEXTMLAFQSR---SLTLSCRTVRP*LRNRNQLCL 598 EESGEH + G GEL+L+ +KDL + + + C TV + + + C Sbjct: 633 TKVEESGEHTILGTGELYLDSIMKDLRELYSEVEVKVADPVVSFCETV---VESSSMKCF 689 Query: 599 SKSPNKHNRLFMKAQPMPDGLPEDI 673 +++PNK N++ M A+P+ GL EDI Sbjct: 690 AETPNKKNKITMIAEPLDRGLAEDI 714 Score = 62.5 bits (145), Expect = 2e-10 Identities = 29/81 (35%), Positives = 46/81 (56%) Frame = +3 Query: 9 VYVSKMVPTSDKGRFYAFGRVFSGKVVTGQKARIMGPNFTPGKKEDLYEKTIQRTILMMG 188 V V+K+ P SD F FGRV+SG++ TGQ R++G ++P +ED+ K + + + Sbjct: 490 VNVTKLYPKSDTSVFDVFGRVYSGRLQTGQSVRVLGEGYSPEDEEDMTIKEVTKLWIYQA 549 Query: 189 RYVEAIEDVPSGNICGLVGVD 251 RY + P G+ + GVD Sbjct: 550 RYRIPVSSAPPGSWVLIEGVD 570 >At1g06220.1 68414.m00655 elongation factor Tu family protein similar to Cryptosporidium parvum elongation factor-2 GB:U21667 GI:706974 from [Cryptosporidium parvum] Length = 987 Score = 100 bits (240), Expect = 8e-22 Identities = 52/145 (35%), Positives = 88/145 (60%), Gaps = 6/145 (4%) Frame = +2 Query: 257 LVKTGTITTF---KNAHNMKVMKFSVSPVVRVAVEPKNPADLPKLVEGLKRLAKSDPMVQ 427 ++KT T+ ++ + + ++F+ PVV+ A EP NP++LPK+VEGL++++KS P+ Sbjct: 573 IMKTATLCNASYDEDVYIFRALQFNTLPVVKTATEPLNPSELPKMVEGLRKISKSYPLAI 632 Query: 428 CINEESGEHIVAGAGELHLEICLKDLEXTMLAFQSR---SLTLSCRTVRP*LRNRNQLCL 598 EESGEH + G GEL+L+ +KDL + + + C TV + + + C Sbjct: 633 TKVEESGEHTILGTGELYLDSIMKDLRELYSEVEVKVADPVVSFCETV---VESSSMKCF 689 Query: 599 SKSPNKHNRLFMKAQPMPDGLPEDI 673 +++PNK N++ M A+P+ GL EDI Sbjct: 690 AETPNKKNKITMIAEPLDRGLAEDI 714 Score = 62.5 bits (145), Expect = 2e-10 Identities = 29/81 (35%), Positives = 46/81 (56%) Frame = +3 Query: 9 VYVSKMVPTSDKGRFYAFGRVFSGKVVTGQKARIMGPNFTPGKKEDLYEKTIQRTILMMG 188 V V+K+ P SD F FGRV+SG++ TGQ R++G ++P +ED+ K + + + Sbjct: 490 VNVTKLYPKSDTSVFDVFGRVYSGRLQTGQSVRVLGEGYSPEDEEDMTIKEVTKLWIYQA 549 Query: 189 RYVEAIEDVPSGNICGLVGVD 251 RY + P G+ + GVD Sbjct: 550 RYRIPVSSAPPGSWVLIEGVD 570 >At3g22980.1 68416.m02898 elongation factor Tu family protein similar to eukaryotic translation elongation factor 2 GB:NP_001952 [Homo sapiens] Length = 1015 Score = 87.0 bits (206), Expect = 1e-17 Identities = 37/82 (45%), Positives = 59/82 (71%) Frame = +2 Query: 263 KTGTITTFKNAHNMKVMKFSVSPVVRVAVEPKNPADLPKLVEGLKRLAKSDPMVQCINEE 442 K+ T+++ +N + M+F VSP +RVA+EP +PAD+ L++GL+ L ++DP V+ Sbjct: 515 KSATLSSTRNCWPLASMEFQVSPTLRVAIEPSDPADMSALMKGLRLLNRADPFVEITVSA 574 Query: 443 SGEHIVAGAGELHLEICLKDLE 508 GEH++A AGE+HLE C+KDL+ Sbjct: 575 RGEHVLAAAGEVHLERCVKDLK 596 Score = 39.1 bits (87), Expect = 0.003 Identities = 21/70 (30%), Positives = 37/70 (52%), Gaps = 4/70 (5%) Frame = +3 Query: 51 FYAFGRVFSGKVVTGQKARIMGPNFTPGKKED----LYEKTIQRTILMMGRYVEAIEDVP 218 F AF R+FSG + GQ+ ++ + P K E + E + LMMG+ + + +V Sbjct: 440 FLAFARIFSGVLRAGQRVFVITALYDPLKGESSHKYIQEAELHSLYLMMGQGLTPVTEVK 499 Query: 219 SGNICGLVGV 248 +GN+ + G+ Sbjct: 500 AGNVVAIRGL 509 >At1g62750.1 68414.m07082 elongation factor Tu family protein similar to elongation factor G SP:P34811 [Glycine max (Soybean)] Length = 783 Score = 57.6 bits (133), Expect = 7e-09 Identities = 31/80 (38%), Positives = 49/80 (61%), Gaps = 1/80 (1%) Frame = +2 Query: 272 TITTFKNAHNMKVMKFSVSPVVRVAVEPKNPADLPKLVEGLKRLAKSDPMVQ-CINEESG 448 T++ +N ++ M F PV++VA+EPK AD+ K+ GL +LA+ DP +EE Sbjct: 475 TLSDPENPVVLERMDFP-DPVIKVAIEPKTKADIDKMATGLIKLAQEDPSFHFSRDEEMN 533 Query: 449 EHIVAGAGELHLEICLKDLE 508 + ++ G GELHLEI + L+ Sbjct: 534 QTVIEGMGELHLEIIVDRLK 553 >At2g45030.1 68415.m05606 mitochondrial elongation factor, putative similar to SP|P25039 Elongation factor G 1, mitochondrial precursor (mEF-G-1) {Saccharomyces cerevisiae}; contains Pfam profiles PF00009: Elongation factor Tu GTP binding domain, PF03764: Elongation factor G domain IV, PF00679: Elongation factor G C-terminus Length = 754 Score = 42.3 bits (95), Expect = 3e-04 Identities = 21/55 (38%), Positives = 34/55 (61%), Gaps = 1/55 (1%) Frame = +2 Query: 329 PVVRVAVEPKNPADLPKLVEGLKRLAKSDPMVQC-INEESGEHIVAGAGELHLEI 490 PV+ +AV+P + + + L R K DP + ++ ESG+ I++G GELHL+I Sbjct: 461 PVMSLAVQPVSKDSGGQFSKALNRFQKEDPTFRVGLDPESGQTIISGMGELHLDI 515 >At1g45332.1 68414.m05195 mitochondrial elongation factor, putative similar to mitochondrial elongation factor GI:3917 from [Saccharomyces cerevisiae] Length = 754 Score = 42.3 bits (95), Expect = 3e-04 Identities = 21/55 (38%), Positives = 34/55 (61%), Gaps = 1/55 (1%) Frame = +2 Query: 329 PVVRVAVEPKNPADLPKLVEGLKRLAKSDPMVQC-INEESGEHIVAGAGELHLEI 490 PV+ +AV+P + + + L R K DP + ++ ESG+ I++G GELHL+I Sbjct: 461 PVMSLAVQPVSKDSGGQFSKALNRFQKEDPTFRVGLDPESGQTIISGMGELHLDI 515 >At2g47500.1 68415.m05929 kinesin motor protein-related Length = 974 Score = 30.3 bits (65), Expect = 1.2 Identities = 14/36 (38%), Positives = 20/36 (55%) Frame = +2 Query: 287 KNAHNMKVMKFSVSPVVRVAVEPKNPADLPKLVEGL 394 +N N S+S +VR + K P D+PKL+E L Sbjct: 218 ENDSNKLSSPSSLSTLVRAVLSDKKPEDVPKLIESL 253 >At1g04650.1 68414.m00462 hypothetical protein Length = 936 Score = 29.1 bits (62), Expect = 2.8 Identities = 16/45 (35%), Positives = 22/45 (48%) Frame = -2 Query: 239 KTTDVTRGHILNSFNITPHHKDCTLDSLLIQVLFLSRCKVWSHDA 105 KTT G +LN+ +T + LDSL + CK SHD+ Sbjct: 334 KTTVDLLGALLNAAELTQEFRLTLLDSLFVDEFSNQICKKQSHDS 378 >At1g24050.1 68414.m03036 expressed protein Length = 173 Score = 28.3 bits (60), Expect = 5.0 Identities = 12/31 (38%), Positives = 19/31 (61%) Frame = +3 Query: 42 KGRFYAFGRVFSGKVVTGQKARIMGPNFTPG 134 +G +A G ++S KV+TG + R +G PG Sbjct: 15 EGEKFAVGNIYSVKVITGDEFRGIGTKPRPG 45 >At5g26210.1 68418.m03119 PHD finger family protein contains Pfam domain, PF00628: PHD-finger Length = 255 Score = 27.9 bits (59), Expect = 6.6 Identities = 11/31 (35%), Positives = 18/31 (58%) Frame = +2 Query: 581 RNQLCLSKSPNKHNRLFMKAQPMPDGLPEDI 673 + LCL PN+H + + A+ +P LPE + Sbjct: 45 KENLCLYGHPNEHWEVNLPAEEVPPELPEPV 75 >At4g33440.1 68417.m04751 glycoside hydrolase family 28 protein / polygalacturonase (pectinase) family protein weak similarity to SP|P27644 Polygalacturonase (EC 3.2.1.15) (Pectinase) {Agrobacterium tumefaciens}; contains PF00295: Glycosyl hydrolases family 28 Length = 475 Score = 27.9 bits (59), Expect = 6.6 Identities = 10/36 (27%), Positives = 21/36 (58%) Frame = +2 Query: 218 LW*HLWSCWSRSVLVKTGTITTFKNAHNMKVMKFSV 325 +W LW W+R+++ G + KN+HN+ + ++ Sbjct: 193 MWWELW--WNRTLVHTRGHLIELKNSHNILISNLTL 226 >At3g03405.1 68416.m00338 hypothetical protein temporary automated functional assignment Length = 193 Score = 27.9 bits (59), Expect = 6.6 Identities = 12/36 (33%), Positives = 20/36 (55%) Frame = -1 Query: 558 VRHDRVRLLDWNASMVXSRSLRQISRWSSPAPATMC 451 VR D RL+ WN + +RS++ + W + + A C Sbjct: 27 VRGDNTRLVVWNPYIGQTRSIKLVKGWDTCSYAFGC 62 >At2g41620.1 68415.m05143 nucleoporin interacting component family protein contains Pfam profile PF04097: Nucleoporin interacting component Length = 861 Score = 27.9 bits (59), Expect = 6.6 Identities = 12/39 (30%), Positives = 19/39 (48%) Frame = -2 Query: 125 KVWSHDASFLSGNNLARENASKGVETTFVGGRHHLAHVH 9 K+W + ++ R+ SK + +G RHHL H H Sbjct: 251 KIWQLVQAITGEDSAVRQGVSKRMALA-IGARHHLQHGH 288 >At1g10750.1 68414.m01229 expressed protein similar to gi 3128199 F4I1.5 putative proteinase from Arabidopsis thaliana BAC gb AC004521 Length = 467 Score = 27.9 bits (59), Expect = 6.6 Identities = 19/59 (32%), Positives = 32/59 (54%), Gaps = 1/59 (1%) Frame = -1 Query: 591 SWFRFLSYGLTVRHDRVRLLD-WNASMVXSRSLRQISRWSSPAPATMCSPDSSLIHCTM 418 S+ LS L+ R+ +R LD N ++ LR+I++ P+ T+ SPD +I C + Sbjct: 77 SFSSVLSENLSPRNQTLRPLDELNKLKAINQHLRKINK---PSIKTIHSPDGDIIDCVL 132 >At4g21880.1 68417.m03164 pentatricopeptide (PPR) repeat-containing protein contains Pfam profile PF01535: PPR repeat Length = 845 Score = 27.5 bits (58), Expect = 8.7 Identities = 10/25 (40%), Positives = 15/25 (60%) Frame = +1 Query: 25 WCRPPTKVVSTPLDAFSLARLLPDK 99 WC+P TK+ T L + ++PDK Sbjct: 801 WCKPYTKLTLTLLFVVEVVAIVPDK 825 >At3g47480.1 68416.m05163 calcium-binding EF hand family protein contains INTERPRO:IPR002048 calcium-binding EF-hand domain Length = 183 Score = 27.5 bits (58), Expect = 8.7 Identities = 14/45 (31%), Positives = 27/45 (60%), Gaps = 1/45 (2%) Frame = +2 Query: 329 PVVRVAVEPKNPADLPKLVEGLKRLAK-SDPMVQCINEESGEHIV 460 P+++ V P N AD+ VE +K + S+ + +C+ EE E+++ Sbjct: 63 PIIKKVVVP-NRADIKTSVEEVKAIIDDSEALYECLIEEGEEYLL 106 >At3g06850.2 68416.m00813 branched chain alpha-keto acid dehydrogenase E2 subunit (din3) identical to branched chain alpha-keto acid dehydrogenase E2 subunit (din3) [Arabidopsis thaliana] GI:7021284 Length = 483 Score = 27.5 bits (58), Expect = 8.7 Identities = 18/75 (24%), Positives = 32/75 (42%) Frame = +2 Query: 338 RVAVEPKNPADLPKLVEGLKRLAKSDPMVQCINEESGEHIVAGAGELHLEICLKDLEXTM 517 +VA+ +P D+ K+ E L RLA D + +S E + G + E L L Sbjct: 128 KVALISHSPGDIIKVGETLVRLAVEDSQDSLLTTDSSEIVTLGGSKQGTENLLGALSTPA 187 Query: 518 LAFQSRSLTLSCRTV 562 + ++ L + + Sbjct: 188 VRNLAKDLGIDINVI 202 >At3g06850.1 68416.m00812 branched chain alpha-keto acid dehydrogenase E2 subunit (din3) identical to branched chain alpha-keto acid dehydrogenase E2 subunit (din3) [Arabidopsis thaliana] GI:7021284 Length = 483 Score = 27.5 bits (58), Expect = 8.7 Identities = 18/75 (24%), Positives = 32/75 (42%) Frame = +2 Query: 338 RVAVEPKNPADLPKLVEGLKRLAKSDPMVQCINEESGEHIVAGAGELHLEICLKDLEXTM 517 +VA+ +P D+ K+ E L RLA D + +S E + G + E L L Sbjct: 128 KVALISHSPGDIIKVGETLVRLAVEDSQDSLLTTDSSEIVTLGGSKQGTENLLGALSTPA 187 Query: 518 LAFQSRSLTLSCRTV 562 + ++ L + + Sbjct: 188 VRNLAKDLGIDINVI 202 >At1g51370.2 68414.m05778 F-box family protein contains F-box domain Pfam:PF00646 Length = 346 Score = 27.5 bits (58), Expect = 8.7 Identities = 11/27 (40%), Positives = 13/27 (48%) Frame = -1 Query: 606 DFERQSWFRFLSYGLTVRHDRVRLLDW 526 D R SW R L L HD+ L+ W Sbjct: 83 DSHRDSWIRKLRLDLGYHHDKYDLMSW 109 >At1g51370.1 68414.m05779 F-box family protein contains F-box domain Pfam:PF00646 Length = 435 Score = 27.5 bits (58), Expect = 8.7 Identities = 11/27 (40%), Positives = 13/27 (48%) Frame = -1 Query: 606 DFERQSWFRFLSYGLTVRHDRVRLLDW 526 D R SW R L L HD+ L+ W Sbjct: 83 DSHRDSWIRKLRLDLGYHHDKYDLMSW 109 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 15,620,110 Number of Sequences: 28952 Number of extensions: 326423 Number of successful extensions: 1064 Number of sequences better than 10.0: 22 Number of HSP's better than 10.0 without gapping: 1015 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 1052 length of database: 12,070,560 effective HSP length: 79 effective length of database: 9,783,352 effective search space used: 1438152744 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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