BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= NV060436.seq (598 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC9E9.09c |||aldehyde dehydrogenase|Schizosaccharomyces pombe|... 111 6e-26 SPAC922.07c |||aldehyde dehydrogenase |Schizosaccharomyces pombe... 109 3e-25 SPCC550.10 |meu8||betaine aldehyde dehydrogenase |Schizosaccharo... 91 9e-20 SPAC1002.12c |||succinate-semialdehyde dehydrogenase |Schizosacc... 85 6e-18 SPAC139.05 |||succinate-semialdehyde dehydrogenase |Schizosaccha... 76 3e-15 SPBC24C6.04 |||delta-1-pyrroline-5-carboxylate dehydrogenase|Sch... 48 8e-07 SPBC21C3.15c |||aldehyde dehydrogenase |Schizosaccharomyces pomb... 47 2e-06 SPBC27B12.11c |||transcription factor |Schizosaccharomyces pombe... 26 4.8 SPAC57A7.08 |pzh1||serine/threonine protein phosphatase Pzh1|Sch... 25 6.3 SPAC4F8.14c |hcs1|hcs|3-hydroxy-3-methylglutaryl-CoA synthase|Sc... 25 8.4 >SPAC9E9.09c |||aldehyde dehydrogenase|Schizosaccharomyces pombe|chr 1|||Manual Length = 503 Score = 111 bits (268), Expect = 6e-26 Identities = 51/83 (61%), Positives = 62/83 (74%), Gaps = 1/83 (1%) Frame = +3 Query: 9 GQIIPWNFPILMAAWKLGPALATGCTVVMKPAEQTPLTALYIAQLVKEAGFPPGVVNMLP 188 GQIIPWNFP LM AWK+ PA+A G T+++K AE TPL+AL + + V E GFPPGV+N+L Sbjct: 165 GQIIPWNFPFLMCAWKIAPAVACGNTIILKTAELTPLSALCLTKFVPECGFPPGVINVLS 224 Query: 189 GYG-DTGAAIVDHPDVDKVAFTG 254 G G G AI H D+DKVAFTG Sbjct: 225 GDGRRCGNAISSHMDIDKVAFTG 247 Score = 95.9 bits (228), Expect = 4e-21 Identities = 45/100 (45%), Positives = 63/100 (63%), Gaps = 1/100 (1%) Frame = +2 Query: 254 ATEVGKLIQRGAAST-IKRITLELGGKSPNIVLADTDLPRAVEAAHNALFYNMGQCCXXG 430 +T VG+++ R AAS+ +K++TLELGGKSPNIV D DL A + +FYN GQ C G Sbjct: 248 STGVGRMVMRAAASSNLKKVTLELGGKSPNIVFNDADLDSAAVWTNYGIFYNSGQVCCAG 307 Query: 431 SRTFVXDKIYDQFVELSAERANRRVVGTPFRPXVEQGPQI 550 SR +V + +YD+F++ +A VG PF QG Q+ Sbjct: 308 SRVYVQEDVYDEFIKRMVAKAKTLKVGDPFAEDTFQGAQV 347 >SPAC922.07c |||aldehyde dehydrogenase |Schizosaccharomyces pombe|chr 1|||Manual Length = 496 Score = 109 bits (262), Expect = 3e-25 Identities = 47/83 (56%), Positives = 62/83 (74%), Gaps = 1/83 (1%) Frame = +3 Query: 9 GQIIPWNFPILMAAWKLGPALATGCTVVMKPAEQTPLTALYIAQLVKEAGFPPGVVNMLP 188 GQI+PWN+P+ MA WK+ PALA G +++K AE TPL+ LY A LV+EAGFP GVVN++ Sbjct: 159 GQIVPWNYPLNMAGWKIAPALAAGNCIIIKSAETTPLSLLYFATLVEEAGFPKGVVNIIS 218 Query: 189 GYGD-TGAAIVDHPDVDKVAFTG 254 G G G+ + HP +DK+AFTG Sbjct: 219 GLGTVAGSYMAKHPGIDKIAFTG 241 Score = 87.4 bits (207), Expect = 1e-18 Identities = 43/100 (43%), Positives = 61/100 (61%), Gaps = 1/100 (1%) Frame = +2 Query: 254 ATEVGKLIQRGAASTIKRITLELGGKSPNIVLADTDLPRAVEAAHNALFYNMGQCCXXGS 433 +T+VG ++Q+ AAS +K +TLE GGKSP +V D DL +AV+ A + YN GQ C S Sbjct: 242 STKVGVIVQQLAASNLKAVTLECGGKSPFLVFEDADLDQAVKWAALGIMYNSGQICTSNS 301 Query: 434 RTFVXDKIYDQFVELSAERA-NRRVVGTPFRPXVEQGPQI 550 R +V D +YD+F+EL + +VG PF GP + Sbjct: 302 RIYVQDSVYDKFIELFKKHVIQDYIVGMPFDDNTVVGPVV 341 >SPCC550.10 |meu8||betaine aldehyde dehydrogenase |Schizosaccharomyces pombe|chr 3|||Manual Length = 500 Score = 91.5 bits (217), Expect = 9e-20 Identities = 43/83 (51%), Positives = 58/83 (69%), Gaps = 1/83 (1%) Frame = +3 Query: 9 GQIIPWNFPILMAAWKLGPALATGCTVVMKPAEQTPLTALYIAQLVKEAGFPPGVVNMLP 188 G I PWNFP+ MA WKL PA+A+G VV+KP+E P + L A + KEAG P GV+N++ Sbjct: 167 GVITPWNFPLKMALWKLVPAIASGNCVVLKPSELAPWSCLEFALICKEAGLPDGVLNVII 226 Query: 189 GYG-DTGAAIVDHPDVDKVAFTG 254 G G ++GAA+ HP + +AFTG Sbjct: 227 GSGKESGAALSCHPKIAYLAFTG 249 Score = 76.2 bits (179), Expect = 3e-15 Identities = 37/95 (38%), Positives = 52/95 (54%) Frame = +2 Query: 266 GKLIQRGAASTIKRITLELGGKSPNIVLADTDLPRAVEAAHNALFYNMGQCCXXGSRTFV 445 GK I AA I +TLELGGKSP I+ D DL A+ +A A+F+N G+ C SR V Sbjct: 254 GKKIMHAAAENIVPLTLELGGKSPLIICEDADLSLAIPSAAFAIFFNQGEACTAASRLIV 313 Query: 446 XDKIYDQFVELSAERANRRVVGTPFRPXVEQGPQI 550 + + D+ + AN+ ++G P V GP + Sbjct: 314 HESVADEVLGGLVSEANKLIIGNGLDPQVTLGPVV 348 >SPAC1002.12c |||succinate-semialdehyde dehydrogenase |Schizosaccharomyces pombe|chr 1|||Manual Length = 498 Score = 85.4 bits (202), Expect = 6e-18 Identities = 41/84 (48%), Positives = 55/84 (65%), Gaps = 2/84 (2%) Frame = +3 Query: 9 GQIIPWNFPILMAAWKLGPALATGCTVVMKPAEQTPLTALYIAQLVKEAGFPPGVVNMLP 188 G I PWNFP M K+G ALA GCTVV++PA +TP TAL +A+L + AG P GV+NM+ Sbjct: 164 GIITPWNFPAAMITRKVGAALAAGCTVVIRPAAETPFTALALAKLAERAGVPAGVLNMVT 223 Query: 189 GY--GDTGAAIVDHPDVDKVAFTG 254 + G + +P + KV+FTG Sbjct: 224 ANSPSEHGIELTTNPLIRKVSFTG 247 Score = 80.2 bits (189), Expect = 2e-16 Identities = 38/99 (38%), Positives = 58/99 (58%) Frame = +2 Query: 254 ATEVGKLIQRGAASTIKRITLELGGKSPNIVLADTDLPRAVEAAHNALFYNMGQCCXXGS 433 +T VGK++ + ++ST+K+++LELGG +P IV D DL +A +A F GQ C + Sbjct: 248 STNVGKILAKQSSSTLKKLSLELGGNAPFIVFEDADLEKAADALMACKFRGSGQTCVCAN 307 Query: 434 RTFVXDKIYDQFVELSAERANRRVVGTPFRPXVEQGPQI 550 R +V +YD FV+L ER ++ +G V GP I Sbjct: 308 RIYVHSSVYDAFVDLVTERVSKFKLGYGLDAGVTHGPLI 346 >SPAC139.05 |||succinate-semialdehyde dehydrogenase |Schizosaccharomyces pombe|chr 1|||Manual Length = 493 Score = 76.2 bits (179), Expect = 3e-15 Identities = 38/82 (46%), Positives = 50/82 (60%), Gaps = 2/82 (2%) Frame = +3 Query: 15 IIPWNFPILMAAWKLGPALATGCTVVMKPAEQTPLTALYIAQLVKEAGFPPGVVNMLPGY 194 I PWNFP M A K G ALA GCT + PA +TP L + +L +EAGFP GV+N++ Sbjct: 161 ITPWNFPAAMIARKGGAALAAGCTAIFLPAFRTPYVCLGLVRLAQEAGFPDGVLNVITSS 220 Query: 195 GDT--GAAIVDHPDVDKVAFTG 254 + G + +P V KV+FTG Sbjct: 221 DASAHGKELTTNPIVRKVSFTG 242 Score = 72.5 bits (170), Expect = 4e-14 Identities = 37/99 (37%), Positives = 55/99 (55%) Frame = +2 Query: 254 ATEVGKLIQRGAASTIKRITLELGGKSPNIVLADTDLPRAVEAAHNALFYNMGQCCXXGS 433 +T VGK++ +ASTIK++++ELGG +P IV D + +AVE+ F + GQ C + Sbjct: 243 STNVGKILMGQSASTIKKVSMELGGNAPFIVFPDFPIDQAVESFCTIKFNSCGQVCVCPN 302 Query: 434 RTFVXDKIYDQFVELSAERANRRVVGTPFRPXVEQGPQI 550 R +V +YD+FV E+ VG F GP I Sbjct: 303 RVYVHKNVYDEFVSKLTEKVKTIKVGDGFDSSSAVGPLI 341 >SPBC24C6.04 |||delta-1-pyrroline-5-carboxylate dehydrogenase|Schizosaccharomyces pombe|chr 2|||Manual Length = 548 Score = 48.4 bits (110), Expect = 8e-07 Identities = 25/81 (30%), Positives = 41/81 (50%), Gaps = 1/81 (1%) Frame = +3 Query: 15 IIPWNFPILMAAWKLGPALATGCTVVMKPAEQTPLTALYIAQLVKEAGFPPGVVNMLPGY 194 I P+NF + P L G V+MKP++ L++ + Q+ +EAG P G + +PG Sbjct: 189 ITPFNFTAIAGNLAAAPLLM-GNVVLMKPSDHAVLSSYIVYQIFREAGLPAGALQFIPGD 247 Query: 195 G-DTGAAIVDHPDVDKVAFTG 254 + +HP+ + FTG Sbjct: 248 AVEVSKVCFNHPEFAGLHFTG 268 >SPBC21C3.15c |||aldehyde dehydrogenase |Schizosaccharomyces pombe|chr 2|||Manual Length = 522 Score = 46.8 bits (106), Expect = 2e-06 Identities = 26/96 (27%), Positives = 41/96 (42%) Frame = +2 Query: 263 VGKLIQRGAASTIKRITLELGGKSPNIVLADTDLPRAVEAAHNALFYNMGQCCXXGSRTF 442 + KL+ AA + + LELGGK P I+ D L + +F + GQ C R Sbjct: 221 IAKLVAASAAKQLTPLCLELGGKDPCILTDDHRLEEILSIVMRGVFQSAGQNCIGIERII 280 Query: 443 VXDKIYDQFVELSAERANRRVVGTPFRPXVEQGPQI 550 D +YD + R + +G + V+ G + Sbjct: 281 ALDGVYDTIITKLYNRISTMRLGMYTQNDVDMGAMV 316 Score = 33.5 bits (73), Expect = 0.024 Identities = 21/84 (25%), Positives = 37/84 (44%), Gaps = 4/84 (4%) Frame = +3 Query: 15 IIPWNFPILMAAWKLGPALATGCTVVMKPAEQTP-LTALY---IAQLVKEAGFPPGVVNM 182 ++ WN+P+ A + AL G +V+K +E T T Y + L++ G P +V Sbjct: 134 LVSWNYPLHNALGPIISALFAGNAIVVKGSELTAWSTHQYCEMVRSLLQSMGHSPELVQC 193 Query: 183 LPGYGDTGAAIVDHPDVDKVAFTG 254 + D + H + + F G Sbjct: 194 ITCLPDVADHLTSHSGIKHITFIG 217 >SPBC27B12.11c |||transcription factor |Schizosaccharomyces pombe|chr 2|||Manual Length = 738 Score = 25.8 bits (54), Expect = 4.8 Identities = 12/26 (46%), Positives = 16/26 (61%), Gaps = 3/26 (11%) Frame = -1 Query: 409 HVVEERVV---RGLHGARQVGVGQHD 341 HVVE + RG+HG + G+ QHD Sbjct: 649 HVVERPCIHSLRGIHGQQDGGLEQHD 674 >SPAC57A7.08 |pzh1||serine/threonine protein phosphatase Pzh1|Schizosaccharomyces pombe|chr 1|||Manual Length = 515 Score = 25.4 bits (53), Expect = 6.3 Identities = 12/31 (38%), Positives = 17/31 (54%) Frame = -3 Query: 116 RRLLGRLHDHSTTRRQSWPELPSRHQYREVP 24 + +LG+ D + RR S SRH+Y E P Sbjct: 35 KTVLGKGKDSNHDRRTSTDTTHSRHRYPETP 65 >SPAC4F8.14c |hcs1|hcs|3-hydroxy-3-methylglutaryl-CoA synthase|Schizosaccharomyces pombe|chr 1|||Manual Length = 447 Score = 25.0 bits (52), Expect = 8.4 Identities = 12/31 (38%), Positives = 16/31 (51%), Gaps = 2/31 (6%) Frame = +2 Query: 8 RSDNTMELPYIDG--GLEARASSGDGLYCGH 94 + D T E PY+DG LE + DG Y + Sbjct: 195 KPDLTSEYPYVDGHFSLECYVKALDGAYANY 225 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,095,474 Number of Sequences: 5004 Number of extensions: 36099 Number of successful extensions: 121 Number of sequences better than 10.0: 10 Number of HSP's better than 10.0 without gapping: 109 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 118 length of database: 2,362,478 effective HSP length: 69 effective length of database: 2,017,202 effective search space used: 260219058 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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