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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= NV060432.seq
         (660 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPBC1711.06 |rpl401|rpl4-1, rpl4|60S ribosomal protein L2|Schizo...   116   4e-27
SPBP8B7.03c |rpl402|rpl4-2, rpl4|60S ribosomal protein L2|Schizo...   116   4e-27
SPBC1709.08 |cft1||cleavage factor one Cft1 |Schizosaccharomyces...    27   1.8  
SPAC3G6.04 |rnp24||RNA-binding protein Rnp24|Schizosaccharomyces...    27   3.2  
SPBC577.12 |mug71||endoribonuclease |Schizosaccharomyces pombe|c...    26   4.2  
SPAC1D4.02c |||human GRASP protein homolog |Schizosaccharomyces ...    26   5.5  
SPAC1D4.08 |pis1||CDP-diacylglycerol--inositol 3-phosphatidyltra...    26   5.5  
SPAC3C7.07c |||arginine-tRNA protein transferase |Schizosaccharo...    25   9.7  

>SPBC1711.06 |rpl401|rpl4-1, rpl4|60S ribosomal protein
           L2|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 363

 Score =  116 bits (278), Expect = 4e-27
 Identities = 54/91 (59%), Positives = 56/91 (61%)
 Frame = +3

Query: 255 QTSAESWGTGRAVARIPRVRGGGTHRSGQGAFGNMCRGGRMFAPTKPWRRWHXXXXXXXX 434
           QTSAESWGTGRA+ARIPRV GGGTHRSGQ AFGNMCR GRMFAPTK WR+WH        
Sbjct: 61  QTSAESWGTGRALARIPRVGGGGTHRSGQAAFGNMCRSGRMFAPTKTWRKWHVKVNQNEK 120

Query: 435 XXXXXXXXXXXXXXXXXQARGHIIERFPSFP 527
                             ARGH IE  P  P
Sbjct: 121 RYAIASAVAASGVPSLLLARGHRIEEIPEVP 151



 Score = 77.4 bits (182), Expect = 2e-15
 Identities = 35/62 (56%), Positives = 46/62 (74%)
 Frame = +1

Query: 73  LSVARPLVSVYSEKSETVQGAAKPLPFVFKAPIRPDLVNDVHVSMSKNSRQPYCVSKEAG 252
           ++ ARP VS+Y+ K  +V      LPFVFKAPIRPDLV  VH +++KN RQPY VS++AG
Sbjct: 1   MAAARPTVSIYN-KDGSVSSETLALPFVFKAPIRPDLVRSVHTAVAKNKRQPYAVSEKAG 59

Query: 253 HK 258
           H+
Sbjct: 60  HQ 61



 Score = 44.4 bits (100), Expect = 1e-05
 Identities = 19/35 (54%), Positives = 26/35 (74%)
 Frame = +2

Query: 509 KIPELPLVVADKVQEINKTKQAVIFLRRLKAWSDI 613
           +IPE+PLVV D VQ   KTK+AV  L+ +KA+ D+
Sbjct: 146 EIPEVPLVVDDAVQSFQKTKEAVALLKEIKAYRDV 180


>SPBP8B7.03c |rpl402|rpl4-2, rpl4|60S ribosomal protein
           L2|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 363

 Score =  116 bits (278), Expect = 4e-27
 Identities = 54/91 (59%), Positives = 56/91 (61%)
 Frame = +3

Query: 255 QTSAESWGTGRAVARIPRVRGGGTHRSGQGAFGNMCRGGRMFAPTKPWRRWHXXXXXXXX 434
           QTSAESWGTGRA+ARIPRV GGGTHRSGQ AFGNMCR GRMFAPTK WR+WH        
Sbjct: 61  QTSAESWGTGRALARIPRVGGGGTHRSGQAAFGNMCRSGRMFAPTKTWRKWHVKVNQNEK 120

Query: 435 XXXXXXXXXXXXXXXXXQARGHIIERFPSFP 527
                             ARGH IE  P  P
Sbjct: 121 RYAISSAVAASGVPSLLLARGHRIEEIPEVP 151



 Score = 78.2 bits (184), Expect = 1e-15
 Identities = 36/62 (58%), Positives = 46/62 (74%)
 Frame = +1

Query: 73  LSVARPLVSVYSEKSETVQGAAKPLPFVFKAPIRPDLVNDVHVSMSKNSRQPYCVSKEAG 252
           ++ ARP VS+YS K  +V      LPFVFKAPIRPDLV  VH +++KN RQPY VS++AG
Sbjct: 1   MAAARPTVSIYS-KDGSVSSETIALPFVFKAPIRPDLVRSVHTAVAKNKRQPYAVSEKAG 59

Query: 253 HK 258
           H+
Sbjct: 60  HQ 61



 Score = 44.4 bits (100), Expect = 1e-05
 Identities = 19/35 (54%), Positives = 26/35 (74%)
 Frame = +2

Query: 509 KIPELPLVVADKVQEINKTKQAVIFLRRLKAWSDI 613
           +IPE+PLVV D VQ   KTK+AV  L+ +KA+ D+
Sbjct: 146 EIPEVPLVVDDAVQSFQKTKEAVALLKEIKAYRDV 180


>SPBC1709.08 |cft1||cleavage factor one Cft1 |Schizosaccharomyces
            pombe|chr 2|||Manual
          Length = 1441

 Score = 27.5 bits (58), Expect = 1.8
 Identities = 9/29 (31%), Positives = 19/29 (65%)
 Frame = +1

Query: 211  KNSRQPYCVSKEAGHKPVLNHGVLDVLSP 297
            ++  +PY ++ +  + P+ N G LD++SP
Sbjct: 1054 EDGNEPYAITDDNDYLPMANTGSLDLVSP 1082


>SPAC3G6.04 |rnp24||RNA-binding protein Rnp24|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 369

 Score = 26.6 bits (56), Expect = 3.2
 Identities = 12/47 (25%), Positives = 24/47 (51%)
 Frame = -2

Query: 536 LQPREARESFNNVSSSLNERWDAGSSNGCRQGRSPLSEVDATVPTPP 396
           ++P +  +S NN     +++     S   ++G + + E+D T P PP
Sbjct: 1   MEPIQKMDSLNNKEGQPDKKRKKHESE--KEGENEVLEIDVTKPVPP 45


>SPBC577.12 |mug71||endoribonuclease |Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 606

 Score = 26.2 bits (55), Expect = 4.2
 Identities = 10/26 (38%), Positives = 17/26 (65%)
 Frame = -2

Query: 134 APCTVSLFSEYTDTKGRATDRLISLL 57
           A C V ++SEYT   G ++  L++L+
Sbjct: 490 ADCVVKIWSEYTKNTGESSPVLVALV 515


>SPAC1D4.02c |||human GRASP protein homolog |Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 345

 Score = 25.8 bits (54), Expect = 5.5
 Identities = 12/41 (29%), Positives = 17/41 (41%)
 Frame = -2

Query: 518 RESFNNVSSSLNERWDAGSSNGCRQGRSPLSEVDATVPTPP 396
           R+S   V+   N  W    + GC  G   L  + A +  PP
Sbjct: 178 RDSTRQVTIVPNRHWGGNGAIGCGVGHGVLHRLPAPLSGPP 218


>SPAC1D4.08 |pis1||CDP-diacylglycerol--inositol
           3-phosphatidyltransferase Pis1 |Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 251

 Score = 25.8 bits (54), Expect = 5.5
 Identities = 14/28 (50%), Positives = 20/28 (71%), Gaps = 2/28 (7%)
 Frame = -3

Query: 91  KVG-LPIDSFRYFSEAIPLKY-TLIAVI 14
           K+G LP+ SF Y +  +PL Y TL+AV+
Sbjct: 186 KLGYLPVPSFIYSTGELPLSYPTLLAVL 213


>SPAC3C7.07c |||arginine-tRNA protein transferase
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 361

 Score = 25.0 bits (52), Expect = 9.7
 Identities = 9/22 (40%), Positives = 15/22 (68%), Gaps = 2/22 (9%)
 Frame = -1

Query: 312 GHAEFGRQHV--QYPMIQHWFV 253
           G++ F  +HV   +P ++HWFV
Sbjct: 320 GNSTFPTRHVLSYFPQVKHWFV 341


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,469,360
Number of Sequences: 5004
Number of extensions: 47330
Number of successful extensions: 143
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 133
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 141
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 299817502
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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