BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= NV060432.seq (660 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBC1711.06 |rpl401|rpl4-1, rpl4|60S ribosomal protein L2|Schizo... 116 4e-27 SPBP8B7.03c |rpl402|rpl4-2, rpl4|60S ribosomal protein L2|Schizo... 116 4e-27 SPBC1709.08 |cft1||cleavage factor one Cft1 |Schizosaccharomyces... 27 1.8 SPAC3G6.04 |rnp24||RNA-binding protein Rnp24|Schizosaccharomyces... 27 3.2 SPBC577.12 |mug71||endoribonuclease |Schizosaccharomyces pombe|c... 26 4.2 SPAC1D4.02c |||human GRASP protein homolog |Schizosaccharomyces ... 26 5.5 SPAC1D4.08 |pis1||CDP-diacylglycerol--inositol 3-phosphatidyltra... 26 5.5 SPAC3C7.07c |||arginine-tRNA protein transferase |Schizosaccharo... 25 9.7 >SPBC1711.06 |rpl401|rpl4-1, rpl4|60S ribosomal protein L2|Schizosaccharomyces pombe|chr 2|||Manual Length = 363 Score = 116 bits (278), Expect = 4e-27 Identities = 54/91 (59%), Positives = 56/91 (61%) Frame = +3 Query: 255 QTSAESWGTGRAVARIPRVRGGGTHRSGQGAFGNMCRGGRMFAPTKPWRRWHXXXXXXXX 434 QTSAESWGTGRA+ARIPRV GGGTHRSGQ AFGNMCR GRMFAPTK WR+WH Sbjct: 61 QTSAESWGTGRALARIPRVGGGGTHRSGQAAFGNMCRSGRMFAPTKTWRKWHVKVNQNEK 120 Query: 435 XXXXXXXXXXXXXXXXXQARGHIIERFPSFP 527 ARGH IE P P Sbjct: 121 RYAIASAVAASGVPSLLLARGHRIEEIPEVP 151 Score = 77.4 bits (182), Expect = 2e-15 Identities = 35/62 (56%), Positives = 46/62 (74%) Frame = +1 Query: 73 LSVARPLVSVYSEKSETVQGAAKPLPFVFKAPIRPDLVNDVHVSMSKNSRQPYCVSKEAG 252 ++ ARP VS+Y+ K +V LPFVFKAPIRPDLV VH +++KN RQPY VS++AG Sbjct: 1 MAAARPTVSIYN-KDGSVSSETLALPFVFKAPIRPDLVRSVHTAVAKNKRQPYAVSEKAG 59 Query: 253 HK 258 H+ Sbjct: 60 HQ 61 Score = 44.4 bits (100), Expect = 1e-05 Identities = 19/35 (54%), Positives = 26/35 (74%) Frame = +2 Query: 509 KIPELPLVVADKVQEINKTKQAVIFLRRLKAWSDI 613 +IPE+PLVV D VQ KTK+AV L+ +KA+ D+ Sbjct: 146 EIPEVPLVVDDAVQSFQKTKEAVALLKEIKAYRDV 180 >SPBP8B7.03c |rpl402|rpl4-2, rpl4|60S ribosomal protein L2|Schizosaccharomyces pombe|chr 2|||Manual Length = 363 Score = 116 bits (278), Expect = 4e-27 Identities = 54/91 (59%), Positives = 56/91 (61%) Frame = +3 Query: 255 QTSAESWGTGRAVARIPRVRGGGTHRSGQGAFGNMCRGGRMFAPTKPWRRWHXXXXXXXX 434 QTSAESWGTGRA+ARIPRV GGGTHRSGQ AFGNMCR GRMFAPTK WR+WH Sbjct: 61 QTSAESWGTGRALARIPRVGGGGTHRSGQAAFGNMCRSGRMFAPTKTWRKWHVKVNQNEK 120 Query: 435 XXXXXXXXXXXXXXXXXQARGHIIERFPSFP 527 ARGH IE P P Sbjct: 121 RYAISSAVAASGVPSLLLARGHRIEEIPEVP 151 Score = 78.2 bits (184), Expect = 1e-15 Identities = 36/62 (58%), Positives = 46/62 (74%) Frame = +1 Query: 73 LSVARPLVSVYSEKSETVQGAAKPLPFVFKAPIRPDLVNDVHVSMSKNSRQPYCVSKEAG 252 ++ ARP VS+YS K +V LPFVFKAPIRPDLV VH +++KN RQPY VS++AG Sbjct: 1 MAAARPTVSIYS-KDGSVSSETIALPFVFKAPIRPDLVRSVHTAVAKNKRQPYAVSEKAG 59 Query: 253 HK 258 H+ Sbjct: 60 HQ 61 Score = 44.4 bits (100), Expect = 1e-05 Identities = 19/35 (54%), Positives = 26/35 (74%) Frame = +2 Query: 509 KIPELPLVVADKVQEINKTKQAVIFLRRLKAWSDI 613 +IPE+PLVV D VQ KTK+AV L+ +KA+ D+ Sbjct: 146 EIPEVPLVVDDAVQSFQKTKEAVALLKEIKAYRDV 180 >SPBC1709.08 |cft1||cleavage factor one Cft1 |Schizosaccharomyces pombe|chr 2|||Manual Length = 1441 Score = 27.5 bits (58), Expect = 1.8 Identities = 9/29 (31%), Positives = 19/29 (65%) Frame = +1 Query: 211 KNSRQPYCVSKEAGHKPVLNHGVLDVLSP 297 ++ +PY ++ + + P+ N G LD++SP Sbjct: 1054 EDGNEPYAITDDNDYLPMANTGSLDLVSP 1082 >SPAC3G6.04 |rnp24||RNA-binding protein Rnp24|Schizosaccharomyces pombe|chr 1|||Manual Length = 369 Score = 26.6 bits (56), Expect = 3.2 Identities = 12/47 (25%), Positives = 24/47 (51%) Frame = -2 Query: 536 LQPREARESFNNVSSSLNERWDAGSSNGCRQGRSPLSEVDATVPTPP 396 ++P + +S NN +++ S ++G + + E+D T P PP Sbjct: 1 MEPIQKMDSLNNKEGQPDKKRKKHESE--KEGENEVLEIDVTKPVPP 45 >SPBC577.12 |mug71||endoribonuclease |Schizosaccharomyces pombe|chr 2|||Manual Length = 606 Score = 26.2 bits (55), Expect = 4.2 Identities = 10/26 (38%), Positives = 17/26 (65%) Frame = -2 Query: 134 APCTVSLFSEYTDTKGRATDRLISLL 57 A C V ++SEYT G ++ L++L+ Sbjct: 490 ADCVVKIWSEYTKNTGESSPVLVALV 515 >SPAC1D4.02c |||human GRASP protein homolog |Schizosaccharomyces pombe|chr 1|||Manual Length = 345 Score = 25.8 bits (54), Expect = 5.5 Identities = 12/41 (29%), Positives = 17/41 (41%) Frame = -2 Query: 518 RESFNNVSSSLNERWDAGSSNGCRQGRSPLSEVDATVPTPP 396 R+S V+ N W + GC G L + A + PP Sbjct: 178 RDSTRQVTIVPNRHWGGNGAIGCGVGHGVLHRLPAPLSGPP 218 >SPAC1D4.08 |pis1||CDP-diacylglycerol--inositol 3-phosphatidyltransferase Pis1 |Schizosaccharomyces pombe|chr 1|||Manual Length = 251 Score = 25.8 bits (54), Expect = 5.5 Identities = 14/28 (50%), Positives = 20/28 (71%), Gaps = 2/28 (7%) Frame = -3 Query: 91 KVG-LPIDSFRYFSEAIPLKY-TLIAVI 14 K+G LP+ SF Y + +PL Y TL+AV+ Sbjct: 186 KLGYLPVPSFIYSTGELPLSYPTLLAVL 213 >SPAC3C7.07c |||arginine-tRNA protein transferase |Schizosaccharomyces pombe|chr 1|||Manual Length = 361 Score = 25.0 bits (52), Expect = 9.7 Identities = 9/22 (40%), Positives = 15/22 (68%), Gaps = 2/22 (9%) Frame = -1 Query: 312 GHAEFGRQHV--QYPMIQHWFV 253 G++ F +HV +P ++HWFV Sbjct: 320 GNSTFPTRHVLSYFPQVKHWFV 341 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,469,360 Number of Sequences: 5004 Number of extensions: 47330 Number of successful extensions: 143 Number of sequences better than 10.0: 8 Number of HSP's better than 10.0 without gapping: 133 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 141 length of database: 2,362,478 effective HSP length: 70 effective length of database: 2,012,198 effective search space used: 299817502 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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