Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= NV060432.seq
(660 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g02870.1 68418.m00230 60S ribosomal protein L4/L1 (RPL4D) 60S... 118 3e-27
At3g09630.1 68416.m01142 60S ribosomal protein L4/L1 (RPL4A) str... 118 3e-27
At1g57750.1 68414.m06552 cytochrome P450, putative similar to cy... 31 0.90
At1g26400.1 68414.m03220 FAD-binding domain-containing protein s... 30 1.6
At5g55100.2 68418.m06869 SWAP (Suppressor-of-White-APricot)/surp... 29 2.7
At5g55100.1 68418.m06868 SWAP (Suppressor-of-White-APricot)/surp... 29 2.7
At5g07520.1 68418.m00861 glycine-rich protein (GRP18) Oleosin; g... 29 3.6
At4g39480.1 68417.m05585 cytochrome P450 family protein contains... 29 3.6
At3g10310.1 68416.m01237 kinesin motor protein-related similar t... 29 3.6
At2g43680.2 68415.m05430 calmodulin-binding family protein simil... 29 3.6
At2g43680.1 68415.m05429 calmodulin-binding family protein simil... 29 3.6
At5g17650.1 68418.m02069 glycine/proline-rich protein glycine/pr... 28 6.3
At3g12430.1 68416.m01548 expressed protein ; expression supporte... 28 6.3
At3g50110.1 68416.m05478 phosphatase-related similar to PTEN1 GI... 27 8.4
At3g18640.1 68416.m02368 zinc finger protein-related contains si... 27 8.4
At1g80480.1 68414.m09427 PRLI-interacting factor L, putative sim... 27 8.4
At1g76010.1 68414.m08825 expressed protein 27 8.4
At1g48410.2 68414.m05409 argonaute protein (AGO1) identical to S... 27 8.4
At1g48410.1 68414.m05408 argonaute protein (AGO1) identical to S... 27 8.4
>At5g02870.1 68418.m00230 60S ribosomal protein L4/L1 (RPL4D) 60S
roibosomal protein L4, Arabidopsis thaliana,
EMBL:CAA79104
Length = 407
Score = 118 bits (284), Expect = 3e-27
Identities = 55/91 (60%), Positives = 57/91 (62%)
Frame = +3
Query: 255 QTSAESWGTGRAVARIPRVRGGGTHRSGQGAFGNMCRGGRMFAPTKPWRRWHXXXXXXXX 434
QTSAESWGTGRAV+RIPRV GGGTHR+GQ AFGNMCRGGRMFAPTK WRRWH
Sbjct: 67 QTSAESWGTGRAVSRIPRVPGGGTHRAGQAAFGNMCRGGRMFAPTKIWRRWHRRVNVNMK 126
Query: 435 XXXXXXXXXXXXXXXXXQARGHIIERFPSFP 527
ARGH IE P P
Sbjct: 127 RHAIVSAIAATAVPALVMARGHKIENVPEMP 157
Score = 66.1 bits (154), Expect = 2e-11
Identities = 33/63 (52%), Positives = 41/63 (65%), Gaps = 2/63 (3%)
Frame = +1
Query: 76 SVARPLVSVYSEKSE--TVQGAAKPLPFVFKAPIRPDLVNDVHVSMSKNSRQPYCVSKEA 249
+ ARPLV+V + T Q LP V AP+RPD+VN VH +S NSRQPY VSK+A
Sbjct: 5 AAARPLVTVQGLDGDMSTDQSTTVTLPDVMTAPVRPDIVNFVHAQISNNSRQPYAVSKKA 64
Query: 250 GHK 258
GH+
Sbjct: 65 GHQ 67
Score = 35.5 bits (78), Expect = 0.032
Identities = 13/33 (39%), Positives = 23/33 (69%)
Frame = +2
Query: 512 IPELPLVVADKVQEINKTKQAVIFLRRLKAWSD 610
+PE+PLVV+D + + KT A+ L+++ A+ D
Sbjct: 153 VPEMPLVVSDSAEAVEKTSAAIKVLKQIGAYDD 185
>At3g09630.1 68416.m01142 60S ribosomal protein L4/L1 (RPL4A) strong
similarity to 60S ribosomal protein L1 GB:P49691
Length = 406
Score = 118 bits (284), Expect = 3e-27
Identities = 55/91 (60%), Positives = 57/91 (62%)
Frame = +3
Query: 255 QTSAESWGTGRAVARIPRVRGGGTHRSGQGAFGNMCRGGRMFAPTKPWRRWHXXXXXXXX 434
QTSAESWGTGRAV+RIPRV GGGTHR+GQ AFGNMCRGGRMFAPTK WRRWH
Sbjct: 66 QTSAESWGTGRAVSRIPRVPGGGTHRAGQAAFGNMCRGGRMFAPTKIWRRWHRRVNVNMK 125
Query: 435 XXXXXXXXXXXXXXXXXQARGHIIERFPSFP 527
ARGH IE P P
Sbjct: 126 RHAIVSAIAATAVPALVMARGHKIENVPEMP 156
Score = 68.1 bits (159), Expect = 5e-12
Identities = 33/66 (50%), Positives = 45/66 (68%), Gaps = 2/66 (3%)
Frame = +1
Query: 67 MSLSVARPLVSVYSEKSE--TVQGAAKPLPFVFKAPIRPDLVNDVHVSMSKNSRQPYCVS 240
M+ + ARPLV++ + + T Q + LP V AP+RPD+VN VH +S NSRQPY VS
Sbjct: 1 MAAAAARPLVTIQTLDGDMSTDQSSTVVLPDVMTAPVRPDIVNFVHAQISNNSRQPYAVS 60
Query: 241 KEAGHK 258
K+AGH+
Sbjct: 61 KKAGHQ 66
Score = 35.5 bits (78), Expect = 0.032
Identities = 13/33 (39%), Positives = 23/33 (69%)
Frame = +2
Query: 512 IPELPLVVADKVQEINKTKQAVIFLRRLKAWSD 610
+PE+PLVV+D + + KT A+ L+++ A+ D
Sbjct: 152 VPEMPLVVSDSAEAVEKTSAAIKVLKQIGAYDD 184
>At1g57750.1 68414.m06552 cytochrome P450, putative similar to
cytochrome P450 GI:4688670 from [Catharanthus roseus]
Length = 497
Score = 30.7 bits (66), Expect = 0.90
Identities = 21/70 (30%), Positives = 35/70 (50%)
Frame = +1
Query: 91 LVSVYSEKSETVQGAAKPLPFVFKAPIRPDLVNDVHVSMSKNSRQPYCVSKEAGHKPVLN 270
LV +++ SE+++ PLPF K+P +PD++ H + NS+ C+ + V
Sbjct: 351 LVYLHAALSESMR-LYPPLPFNHKSPAKPDVLPSGH-KVDANSKIVICIYALGRMRSVWG 408
Query: 271 HGVLDVLSPE 300
LD PE
Sbjct: 409 EDALD-FKPE 417
>At1g26400.1 68414.m03220 FAD-binding domain-containing protein
similar to SP|P30986 reticuline oxidase precursor
(Berberine-bridge-forming enzyme) (BBE)
(Tetrahydroprotoberberine synthase) [Eschscholzia
californica]; contains PF01565 FAD binding domain
Length = 527
Score = 29.9 bits (64), Expect = 1.6
Identities = 14/25 (56%), Positives = 17/25 (68%)
Frame = -1
Query: 570 CLVLLISWTLSATTKGSSGIFQ*CV 496
CLVLL+S +A TK SGIF C+
Sbjct: 9 CLVLLVSILRAAVTKPDSGIFTGCL 33
>At5g55100.2 68418.m06869 SWAP (Suppressor-of-White-APricot)/surp
domain-containing protein contains Pfam domain PF01805:
Surp module
Length = 844
Score = 29.1 bits (62), Expect = 2.7
Identities = 12/41 (29%), Positives = 21/41 (51%), Gaps = 1/41 (2%)
Frame = -1
Query: 381 RTYVHHDTCYRRHPDRTY-EYHHHGHAEFGRQHVQYPMIQH 262
R++ H + +H D + E+HHH H R+H ++H
Sbjct: 735 RSHHHRSRKHEKHRDSSDDEHHHHRHRSSRRKHEDSSDVEH 775
>At5g55100.1 68418.m06868 SWAP (Suppressor-of-White-APricot)/surp
domain-containing protein contains Pfam domain PF01805:
Surp module
Length = 843
Score = 29.1 bits (62), Expect = 2.7
Identities = 12/41 (29%), Positives = 21/41 (51%), Gaps = 1/41 (2%)
Frame = -1
Query: 381 RTYVHHDTCYRRHPDRTY-EYHHHGHAEFGRQHVQYPMIQH 262
R++ H + +H D + E+HHH H R+H ++H
Sbjct: 735 RSHHHRSRKHEKHRDSSDDEHHHHRHRSSRRKHEDSSDVEH 775
>At5g07520.1 68418.m00861 glycine-rich protein (GRP18) Oleosin;
glycine-rich protein 18 (GRP18) PMID:11431566;
Length = 228
Score = 28.7 bits (61), Expect = 3.6
Identities = 18/64 (28%), Positives = 30/64 (46%), Gaps = 3/64 (4%)
Frame = +3
Query: 216 LEAALLREQGGWSQTSAESWGTGRAVARIPRVRGGGTHRSGQ---GAFGNMCRGGRMFAP 386
+ AL+R + G ++ +AE + + + ++P G G G+ G FGN GG F
Sbjct: 76 MAVALIRRRMG-AKPTAEGTSSAQPLLKLPVYGGYGGFWGGKKFSGTFGNKPGGGNPFGD 134
Query: 387 TKPW 398
W
Sbjct: 135 ISKW 138
>At4g39480.1 68417.m05585 cytochrome P450 family protein contains
Pfam profile: PF00067 cytochrome P450
Length = 989
Score = 28.7 bits (61), Expect = 3.6
Identities = 16/53 (30%), Positives = 26/53 (49%)
Frame = +1
Query: 142 PLPFVFKAPIRPDLVNDVHVSMSKNSRQPYCVSKEAGHKPVLNHGVLDVLSPE 300
P+PF K+P +PD++ H + NSR +C+ K V ++ PE
Sbjct: 384 PVPFNHKSPAKPDVLPSGH-KVKANSRILFCLYSLGRMKSVWGEDAME-FKPE 434
Score = 28.7 bits (61), Expect = 3.6
Identities = 16/53 (30%), Positives = 26/53 (49%)
Frame = +1
Query: 142 PLPFVFKAPIRPDLVNDVHVSMSKNSRQPYCVSKEAGHKPVLNHGVLDVLSPE 300
P+PF K+P +PD++ H + NS+ +C+ K V L+ PE
Sbjct: 857 PVPFQHKSPTKPDVLPSGH-KVDANSKILFCLYSLGRMKSVWGEDALE-FKPE 907
>At3g10310.1 68416.m01237 kinesin motor protein-related similar to
carboxy-terminal kinesin 2 GB:P79955 [Xenopus laevis]
Length = 897
Score = 28.7 bits (61), Expect = 3.6
Identities = 18/46 (39%), Positives = 25/46 (54%), Gaps = 2/46 (4%)
Frame = -2
Query: 518 RESFNNVSSSLN-ERWDAGSSNGCRQGRSPLSEVDATVP-TPPGLR 387
+E N+ +L E W+ SNG ++ +SP S AT TPP LR
Sbjct: 645 KEQIENLKRALGTEEWN-NVSNGSKEIKSPFSRPIATTERTPPRLR 689
>At2g43680.2 68415.m05430 calmodulin-binding family protein similar
to SF16 protein [Helianthus annuus] GI:560150; contains
Pfam profile PF00612: IQ calmodulin-binding motif
Length = 669
Score = 28.7 bits (61), Expect = 3.6
Identities = 13/33 (39%), Positives = 16/33 (48%)
Frame = +2
Query: 365 WWTYVRPHEALAALAPSRQPPTAESGLGGSRCC 463
WW +V LA+ APS P + L SR C
Sbjct: 465 WWNWVDRQNPLASPAPSYSQPQRDFRLTPSRLC 497
>At2g43680.1 68415.m05429 calmodulin-binding family protein similar
to SF16 protein [Helianthus annuus] GI:560150; contains
Pfam profile PF00612: IQ calmodulin-binding motif
Length = 668
Score = 28.7 bits (61), Expect = 3.6
Identities = 13/33 (39%), Positives = 16/33 (48%)
Frame = +2
Query: 365 WWTYVRPHEALAALAPSRQPPTAESGLGGSRCC 463
WW +V LA+ APS P + L SR C
Sbjct: 464 WWNWVDRQNPLASPAPSYSQPQRDFRLTPSRLC 496
>At5g17650.1 68418.m02069 glycine/proline-rich protein
glycine/proline-rich protein GPRP - Arabidopsis
thaliana, EMBL:X84315
Length = 173
Score = 27.9 bits (59), Expect = 6.3
Identities = 10/24 (41%), Positives = 12/24 (50%)
Frame = -1
Query: 369 HHDTCYRRHPDRTYEYHHHGHAEF 298
HH Y H Y Y +HGH +F
Sbjct: 122 HHHGHYGHHHGHGYGYGYHGHGKF 145
>At3g12430.1 68416.m01548 expressed protein ; expression supported
by MPSS
Length = 238
Score = 27.9 bits (59), Expect = 6.3
Identities = 12/40 (30%), Positives = 22/40 (55%), Gaps = 2/40 (5%)
Frame = -3
Query: 145 GAWLHPAPSHSSLNTPTLKVGLPID--SFRYFSEAIPLKY 32
G W+H SH+ ++ L VG+ + Y+S + P++Y
Sbjct: 38 GQWIHDVLSHNRFSSHPLVVGVGVQWTPSSYYSASSPVRY 77
>At3g50110.1 68416.m05478 phosphatase-related similar to PTEN1
GI:5566292 from [Drosophila melanogaster]; contains
prosite evidence: PS00383: Tyrosine specific protein
phosphatases active site
Length = 632
Score = 27.5 bits (58), Expect = 8.4
Identities = 16/45 (35%), Positives = 21/45 (46%)
Frame = -2
Query: 527 REARESFNNVSSSLNERWDAGSSNGCRQGRSPLSEVDATVPTPPG 393
REA E + +S+ + D G PLS+ D VP PPG
Sbjct: 42 REAHEDSISTEASV-AKVDDTQMPASSTGSEPLSKTDDIVPCPPG 85
>At3g18640.1 68416.m02368 zinc finger protein-related contains
similarity to zinc finger proteins (CCCH type)
Length = 676
Score = 27.5 bits (58), Expect = 8.4
Identities = 12/28 (42%), Positives = 16/28 (57%)
Frame = -2
Query: 536 LQPREARESFNNVSSSLNERWDAGSSNG 453
+QP +A N VSS+ N+ W G S G
Sbjct: 488 VQPNQATTQSNVVSSNPNQLWGLGMSTG 515
>At1g80480.1 68414.m09427 PRLI-interacting factor L, putative
similar to PRLI-interacting factor L [Arabidopsis
thaliana] GI:11139268; contains Pfam profile PF02492:
Cobalamin synthesis protein/P47K
Length = 444
Score = 27.5 bits (58), Expect = 8.4
Identities = 9/28 (32%), Positives = 13/28 (46%)
Frame = -1
Query: 369 HHDTCYRRHPDRTYEYHHHGHAEFGRQH 286
HHD + H D +++ HH H H
Sbjct: 311 HHDHDHDHHHDHNHDHDHHHHDGHDHHH 338
>At1g76010.1 68414.m08825 expressed protein
Length = 350
Score = 27.5 bits (58), Expect = 8.4
Identities = 17/43 (39%), Positives = 18/43 (41%)
Frame = +3
Query: 240 QGGWSQTSAESWGTGRAVARIPRVRGGGTHRSGQGAFGNMCRG 368
QG + G GR R R RGGG R G G F N G
Sbjct: 303 QGRGGYDGPQGRGRGRGRGRGGRGRGGG--RGGDGGFNNRSDG 343
>At1g48410.2 68414.m05409 argonaute protein (AGO1) identical to
SP|O04379 Argonaute protein (AGO1) {Arabidopsis
thaliana}; contains Pfam profiles PF02171: Piwi domain,
PF02170: PAZ domain
Length = 1050
Score = 27.5 bits (58), Expect = 8.4
Identities = 15/45 (33%), Positives = 22/45 (48%)
Frame = +3
Query: 240 QGGWSQTSAESWGTGRAVARIPRVRGGGTHRSGQGAFGNMCRGGR 374
+G S+ + G GR R +GGG H+ G+G +GGR
Sbjct: 15 EGSGSREAGPVSGGGRGSQRGGFQQGGGQHQGGRGYTPQPQQGGR 59
>At1g48410.1 68414.m05408 argonaute protein (AGO1) identical to
SP|O04379 Argonaute protein (AGO1) {Arabidopsis
thaliana}; contains Pfam profiles PF02171: Piwi domain,
PF02170: PAZ domain
Length = 1048
Score = 27.5 bits (58), Expect = 8.4
Identities = 15/45 (33%), Positives = 22/45 (48%)
Frame = +3
Query: 240 QGGWSQTSAESWGTGRAVARIPRVRGGGTHRSGQGAFGNMCRGGR 374
+G S+ + G GR R +GGG H+ G+G +GGR
Sbjct: 15 EGSGSREAGPVSGGGRGSQRGGFQQGGGQHQGGRGYTPQPQQGGR 59
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 13,549,802
Number of Sequences: 28952
Number of extensions: 282220
Number of successful extensions: 977
Number of sequences better than 10.0: 19
Number of HSP's better than 10.0 without gapping: 899
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 965
length of database: 12,070,560
effective HSP length: 78
effective length of database: 9,812,304
effective search space used: 1383534864
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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