Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= NV060431.seq
(673 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g02870.1 68418.m00230 60S ribosomal protein L4/L1 (RPL4D) 60S... 111 4e-25
At3g09630.1 68416.m01142 60S ribosomal protein L4/L1 (RPL4A) str... 111 4e-25
At1g57750.1 68414.m06552 cytochrome P450, putative similar to cy... 31 0.92
At1g26400.1 68414.m03220 FAD-binding domain-containing protein s... 30 1.6
At5g55100.2 68418.m06869 SWAP (Suppressor-of-White-APricot)/surp... 29 2.8
At5g55100.1 68418.m06868 SWAP (Suppressor-of-White-APricot)/surp... 29 2.8
At5g07520.1 68418.m00861 glycine-rich protein (GRP18) Oleosin; g... 29 3.7
At4g39480.1 68417.m05585 cytochrome P450 family protein contains... 29 3.7
At5g17650.1 68418.m02069 glycine/proline-rich protein glycine/pr... 28 6.5
At3g12430.1 68416.m01548 expressed protein ; expression supporte... 28 6.5
At4g29230.1 68417.m04181 no apical meristem (NAM) family protein... 27 8.6
At1g80480.1 68414.m09427 PRLI-interacting factor L, putative sim... 27 8.6
At1g76010.1 68414.m08825 expressed protein 27 8.6
At1g48410.2 68414.m05409 argonaute protein (AGO1) identical to S... 27 8.6
At1g48410.1 68414.m05408 argonaute protein (AGO1) identical to S... 27 8.6
>At5g02870.1 68418.m00230 60S ribosomal protein L4/L1 (RPL4D) 60S
roibosomal protein L4, Arabidopsis thaliana,
EMBL:CAA79104
Length = 407
Score = 111 bits (267), Expect = 4e-25
Identities = 47/52 (90%), Positives = 49/52 (94%)
Frame = +1
Query: 256 QTSAESWGTGRAVARIPRVRGGGTHRSGQGAFGNMCRGGRMFAPTKPWRRWH 411
QTSAESWGTGRAV+RIPRV GGGTHR+GQ AFGNMCRGGRMFAPTK WRRWH
Sbjct: 67 QTSAESWGTGRAVSRIPRVPGGGTHRAGQAAFGNMCRGGRMFAPTKIWRRWH 118
Score = 62.5 bits (145), Expect = 2e-10
Identities = 32/61 (52%), Positives = 39/61 (63%), Gaps = 2/61 (3%)
Frame = +2
Query: 77 SVARPLVSVYSEKSE--TVQGAAKPLPFVFKAPIRPDLVNDVHVSMSKNSRQPYCVSKEA 250
+ ARPLV+V + T Q LP V AP+RPD+VN VH +S NSRQPY VSK+A
Sbjct: 5 AAARPLVTVQGLDGDMSTDQSTTVTLPDVMTAPVRPDIVNFVHAQISNNSRQPYAVSKKA 64
Query: 251 G 253
G
Sbjct: 65 G 65
Score = 37.1 bits (82), Expect = 0.011
Identities = 16/25 (64%), Positives = 18/25 (72%)
Frame = +3
Query: 453 SVAATGVPALVQARGHIIERFPSFP 527
++AAT VPALV ARGH IE P P
Sbjct: 133 AIAATAVPALVMARGHKIENVPEMP 157
Score = 30.3 bits (65), Expect = 1.2
Identities = 11/26 (42%), Positives = 18/26 (69%)
Frame = +2
Query: 512 IPELPLVVADKVQEINKTKQAVIFLR 589
+PE+PLVV+D + + KT A+ L+
Sbjct: 153 VPEMPLVVSDSAEAVEKTSAAIKVLK 178
>At3g09630.1 68416.m01142 60S ribosomal protein L4/L1 (RPL4A) strong
similarity to 60S ribosomal protein L1 GB:P49691
Length = 406
Score = 111 bits (267), Expect = 4e-25
Identities = 47/52 (90%), Positives = 49/52 (94%)
Frame = +1
Query: 256 QTSAESWGTGRAVARIPRVRGGGTHRSGQGAFGNMCRGGRMFAPTKPWRRWH 411
QTSAESWGTGRAV+RIPRV GGGTHR+GQ AFGNMCRGGRMFAPTK WRRWH
Sbjct: 66 QTSAESWGTGRAVSRIPRVPGGGTHRAGQAAFGNMCRGGRMFAPTKIWRRWH 117
Score = 64.5 bits (150), Expect = 6e-11
Identities = 32/64 (50%), Positives = 43/64 (67%), Gaps = 2/64 (3%)
Frame = +2
Query: 68 MSLSVARPLVSVYSEKSE--TVQGAAKPLPFVFKAPIRPDLVNDVHVSMSKNSRQPYCVS 241
M+ + ARPLV++ + + T Q + LP V AP+RPD+VN VH +S NSRQPY VS
Sbjct: 1 MAAAAARPLVTIQTLDGDMSTDQSSTVVLPDVMTAPVRPDIVNFVHAQISNNSRQPYAVS 60
Query: 242 KEAG 253
K+AG
Sbjct: 61 KKAG 64
Score = 37.1 bits (82), Expect = 0.011
Identities = 16/25 (64%), Positives = 18/25 (72%)
Frame = +3
Query: 453 SVAATGVPALVQARGHIIERFPSFP 527
++AAT VPALV ARGH IE P P
Sbjct: 132 AIAATAVPALVMARGHKIENVPEMP 156
Score = 30.3 bits (65), Expect = 1.2
Identities = 11/26 (42%), Positives = 18/26 (69%)
Frame = +2
Query: 512 IPELPLVVADKVQEINKTKQAVIFLR 589
+PE+PLVV+D + + KT A+ L+
Sbjct: 152 VPEMPLVVSDSAEAVEKTSAAIKVLK 177
>At1g57750.1 68414.m06552 cytochrome P450, putative similar to
cytochrome P450 GI:4688670 from [Catharanthus roseus]
Length = 497
Score = 30.7 bits (66), Expect = 0.92
Identities = 21/70 (30%), Positives = 35/70 (50%)
Frame = +2
Query: 92 LVSVYSEKSETVQGAAKPLPFVFKAPIRPDLVNDVHVSMSKNSRQPYCVSKEAGPKPVLN 271
LV +++ SE+++ PLPF K+P +PD++ H + NS+ C+ + V
Sbjct: 351 LVYLHAALSESMR-LYPPLPFNHKSPAKPDVLPSGH-KVDANSKIVICIYALGRMRSVWG 408
Query: 272 HGVLDVLSPE 301
LD PE
Sbjct: 409 EDALD-FKPE 417
>At1g26400.1 68414.m03220 FAD-binding domain-containing protein
similar to SP|P30986 reticuline oxidase precursor
(Berberine-bridge-forming enzyme) (BBE)
(Tetrahydroprotoberberine synthase) [Eschscholzia
californica]; contains PF01565 FAD binding domain
Length = 527
Score = 29.9 bits (64), Expect = 1.6
Identities = 14/25 (56%), Positives = 17/25 (68%)
Frame = -2
Query: 570 CLVLLISWTLSATTKGSSGIFQ*CV 496
CLVLL+S +A TK SGIF C+
Sbjct: 9 CLVLLVSILRAAVTKPDSGIFTGCL 33
>At5g55100.2 68418.m06869 SWAP (Suppressor-of-White-APricot)/surp
domain-containing protein contains Pfam domain PF01805:
Surp module
Length = 844
Score = 29.1 bits (62), Expect = 2.8
Identities = 12/41 (29%), Positives = 21/41 (51%), Gaps = 1/41 (2%)
Frame = -1
Query: 382 RTYVHHDTCYRRHPDRTY-EYHHHGHAEFGRQHVQYPMIQH 263
R++ H + +H D + E+HHH H R+H ++H
Sbjct: 735 RSHHHRSRKHEKHRDSSDDEHHHHRHRSSRRKHEDSSDVEH 775
>At5g55100.1 68418.m06868 SWAP (Suppressor-of-White-APricot)/surp
domain-containing protein contains Pfam domain PF01805:
Surp module
Length = 843
Score = 29.1 bits (62), Expect = 2.8
Identities = 12/41 (29%), Positives = 21/41 (51%), Gaps = 1/41 (2%)
Frame = -1
Query: 382 RTYVHHDTCYRRHPDRTY-EYHHHGHAEFGRQHVQYPMIQH 263
R++ H + +H D + E+HHH H R+H ++H
Sbjct: 735 RSHHHRSRKHEKHRDSSDDEHHHHRHRSSRRKHEDSSDVEH 775
>At5g07520.1 68418.m00861 glycine-rich protein (GRP18) Oleosin;
glycine-rich protein 18 (GRP18) PMID:11431566;
Length = 228
Score = 28.7 bits (61), Expect = 3.7
Identities = 18/64 (28%), Positives = 30/64 (46%), Gaps = 3/64 (4%)
Frame = +1
Query: 217 LEAALLREQGGWSQTSAESWGTGRAVARIPRVRGGGTHRSGQ---GAFGNMCRGGRMFAP 387
+ AL+R + G ++ +AE + + + ++P G G G+ G FGN GG F
Sbjct: 76 MAVALIRRRMG-AKPTAEGTSSAQPLLKLPVYGGYGGFWGGKKFSGTFGNKPGGGNPFGD 134
Query: 388 TKPW 399
W
Sbjct: 135 ISKW 138
>At4g39480.1 68417.m05585 cytochrome P450 family protein contains
Pfam profile: PF00067 cytochrome P450
Length = 989
Score = 28.7 bits (61), Expect = 3.7
Identities = 16/53 (30%), Positives = 26/53 (49%)
Frame = +2
Query: 143 PLPFVFKAPIRPDLVNDVHVSMSKNSRQPYCVSKEAGPKPVLNHGVLDVLSPE 301
P+PF K+P +PD++ H + NSR +C+ K V ++ PE
Sbjct: 384 PVPFNHKSPAKPDVLPSGH-KVKANSRILFCLYSLGRMKSVWGEDAME-FKPE 434
Score = 28.7 bits (61), Expect = 3.7
Identities = 16/53 (30%), Positives = 26/53 (49%)
Frame = +2
Query: 143 PLPFVFKAPIRPDLVNDVHVSMSKNSRQPYCVSKEAGPKPVLNHGVLDVLSPE 301
P+PF K+P +PD++ H + NS+ +C+ K V L+ PE
Sbjct: 857 PVPFQHKSPTKPDVLPSGH-KVDANSKILFCLYSLGRMKSVWGEDALE-FKPE 907
>At5g17650.1 68418.m02069 glycine/proline-rich protein
glycine/proline-rich protein GPRP - Arabidopsis
thaliana, EMBL:X84315
Length = 173
Score = 27.9 bits (59), Expect = 6.5
Identities = 10/24 (41%), Positives = 12/24 (50%)
Frame = -1
Query: 370 HHDTCYRRHPDRTYEYHHHGHAEF 299
HH Y H Y Y +HGH +F
Sbjct: 122 HHHGHYGHHHGHGYGYGYHGHGKF 145
>At3g12430.1 68416.m01548 expressed protein ; expression supported
by MPSS
Length = 238
Score = 27.9 bits (59), Expect = 6.5
Identities = 12/40 (30%), Positives = 22/40 (55%), Gaps = 2/40 (5%)
Frame = -3
Query: 146 GAWLHPAPSHSSLNTPTLKVGLPID--SFRYFSEAIPLKY 33
G W+H SH+ ++ L VG+ + Y+S + P++Y
Sbjct: 38 GQWIHDVLSHNRFSSHPLVVGVGVQWTPSSYYSASSPVRY 77
>At4g29230.1 68417.m04181 no apical meristem (NAM) family protein
similar to NAM family proteins GP|12751304|,
GP|6223650|, GP|9758909 - Arabidopsis
thaliana,PIR2:T04621
Length = 498
Score = 27.5 bits (58), Expect = 8.6
Identities = 17/59 (28%), Positives = 22/59 (37%), Gaps = 4/59 (6%)
Frame = -1
Query: 349 RHPDRTYEYHHHGHAEFGRQHVQYPMIQHWFGTSLLAHAVGL---PRV-LGHRNVNIID 185
RH + HH H H Q H F S H + P L H ++NI+D
Sbjct: 346 RHQPSSSTSHHMAHDHHHHHHQQQQQRHHAFNISQPTHPISTIISPSTSLHHASINILD 404
>At1g80480.1 68414.m09427 PRLI-interacting factor L, putative
similar to PRLI-interacting factor L [Arabidopsis
thaliana] GI:11139268; contains Pfam profile PF02492:
Cobalamin synthesis protein/P47K
Length = 444
Score = 27.5 bits (58), Expect = 8.6
Identities = 9/28 (32%), Positives = 13/28 (46%)
Frame = -1
Query: 370 HHDTCYRRHPDRTYEYHHHGHAEFGRQH 287
HHD + H D +++ HH H H
Sbjct: 311 HHDHDHDHHHDHNHDHDHHHHDGHDHHH 338
>At1g76010.1 68414.m08825 expressed protein
Length = 350
Score = 27.5 bits (58), Expect = 8.6
Identities = 17/43 (39%), Positives = 18/43 (41%)
Frame = +1
Query: 241 QGGWSQTSAESWGTGRAVARIPRVRGGGTHRSGQGAFGNMCRG 369
QG + G GR R R RGGG R G G F N G
Sbjct: 303 QGRGGYDGPQGRGRGRGRGRGGRGRGGG--RGGDGGFNNRSDG 343
>At1g48410.2 68414.m05409 argonaute protein (AGO1) identical to
SP|O04379 Argonaute protein (AGO1) {Arabidopsis
thaliana}; contains Pfam profiles PF02171: Piwi domain,
PF02170: PAZ domain
Length = 1050
Score = 27.5 bits (58), Expect = 8.6
Identities = 15/45 (33%), Positives = 22/45 (48%)
Frame = +1
Query: 241 QGGWSQTSAESWGTGRAVARIPRVRGGGTHRSGQGAFGNMCRGGR 375
+G S+ + G GR R +GGG H+ G+G +GGR
Sbjct: 15 EGSGSREAGPVSGGGRGSQRGGFQQGGGQHQGGRGYTPQPQQGGR 59
>At1g48410.1 68414.m05408 argonaute protein (AGO1) identical to
SP|O04379 Argonaute protein (AGO1) {Arabidopsis
thaliana}; contains Pfam profiles PF02171: Piwi domain,
PF02170: PAZ domain
Length = 1048
Score = 27.5 bits (58), Expect = 8.6
Identities = 15/45 (33%), Positives = 22/45 (48%)
Frame = +1
Query: 241 QGGWSQTSAESWGTGRAVARIPRVRGGGTHRSGQGAFGNMCRGGR 375
+G S+ + G GR R +GGG H+ G+G +GGR
Sbjct: 15 EGSGSREAGPVSGGGRGSQRGGFQQGGGQHQGGRGYTPQPQQGGR 59
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 14,317,872
Number of Sequences: 28952
Number of extensions: 300547
Number of successful extensions: 969
Number of sequences better than 10.0: 15
Number of HSP's better than 10.0 without gapping: 886
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 957
length of database: 12,070,560
effective HSP length: 78
effective length of database: 9,812,304
effective search space used: 1422784080
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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