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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= NV060429.seq
         (675 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces...   158   6e-40
SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|ch...    88   9e-19
SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosacchar...    87   3e-18
SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|...    80   3e-16
SPBC19G7.16 |iws1||transcription elongation factor complex subun...    27   2.5  
SPAC29E6.01 |pof11|SPAC30.05, mug156|F-box protein Pof11|Schizos...    27   3.3  
SPAP32A8.03c |||ubiquitin-protein ligase E3 |Schizosaccharomyces...    26   4.3  
SPBC19C7.11 |||ClC chloride channel |Schizosaccharomyces pombe|c...    26   5.7  
SPAC6C3.08 |||gankyrin|Schizosaccharomyces pombe|chr 1|||Manual        25   7.6  
SPAC8F11.09c |nnt1||nicotinamide N-methyltransferase Nnt1 |Schiz...    25   7.6  
SPBC11C11.11c ||SPBC3B8.12|ATP-dependent DNA helicase Irc3 |Schi...    25   10.0 

>SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 448

 Score =  158 bits (384), Expect = 6e-40
 Identities = 79/134 (58%), Positives = 93/134 (69%)
 Frame = +1

Query: 256 VPRAILVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDV 435
           VPRA+LVDLEPGTMD+V+SG FG +FRPDN ++GQSGAGN WAKGHYTEGAEL D+VLDV
Sbjct: 60  VPRAVLVDLEPGTMDAVKSGKFGNLFRPDNIIYGQSGAGNIWAKGHYTEGAELADAVLDV 119

Query: 436 VRKEAESW*LPPGIPTDTLARRXHRSGMGTLLIFKIREEYPDRIMNTYSVVHHLKCXTQX 615
           VR+EAE+     G            SGMGTLL+ KIREEYPDR+M T+SV    K     
Sbjct: 120 VRREAEACDALQGFQLTHSLGGGTGSGMGTLLLSKIREEYPDRMMATFSVAPAPKSSDTV 179

Query: 616 LXRXTPHFLVHQLV 657
           +        +HQLV
Sbjct: 180 VEPYNATLSMHQLV 193



 Score =  101 bits (243), Expect = 7e-23
 Identities = 42/58 (72%), Positives = 49/58 (84%)
 Frame = +2

Query: 80  MREIVHIQAGQCGNQIGAKFWEIISDEHGIDPTGAYHGDSDLQLERINVYYNEASGGK 253
           MREIVHIQAGQCGNQ+GA FW  I+DEHG+D  G YHG S+ Q ER+NVY+NEA+GGK
Sbjct: 1   MREIVHIQAGQCGNQVGAAFWSTIADEHGLDSAGIYHGTSEAQHERLNVYFNEAAGGK 58


>SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 455

 Score = 88.2 bits (209), Expect = 9e-19
 Identities = 44/110 (40%), Positives = 66/110 (60%)
 Frame = +1

Query: 256 VPRAILVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDV 435
           VPR+I VDLEP  +D VR+GP+  +F P+  V G+  A NN+A+GHYT G E++DSVL+ 
Sbjct: 66  VPRSIYVDLEPNVIDQVRTGPYKDLFHPEQMVTGKEDASNNYARGHYTVGKEMIDSVLER 125

Query: 436 VRKEAESW*LPPGIPTDTLARRXHRSGMGTLLIFKIREEYPDRIMNTYSV 585
           +R+ A++     G            SG+G LL+ ++  EY  +    +SV
Sbjct: 126 IRRMADNCSGLQGFLVFHSFGGGTGSGLGALLLERLNMEYGKKSNLQFSV 175



 Score = 46.4 bits (105), Expect = 4e-06
 Identities = 41/151 (27%), Positives = 56/151 (37%), Gaps = 8/151 (5%)
 Frame = +2

Query: 80  MREIVHIQAGQCGNQIGAKFWEIISDEHGIDPTGAYHGDSDLQLERINVYYNEASGGKSC 259
           MRE++ +  GQ G QIG   WE+   EHGI P G    +S  ++ + N Y N+  G    
Sbjct: 1   MREVISVHVGQAGVQIGNACWELYCLEHGIGPDGFPTENS--EVHKNNSYLNDGFGTFFS 58

Query: 260 XXXXXXXXXXXXXXLSALDLXXXXXXXXXXXXXXPAPVTTGPRDTTRRVLSSSI-----Q 424
                             ++              P  + TG  D +              
Sbjct: 59  ETGQGKFVPRSIYVDLEPNVIDQVRTGPYKDLFHPEQMVTGKEDASNNYARGHYTVGKEM 118

Query: 425 F*TSFERKRNRGD-C--LQGFQLTHSLGGGT 508
             +  ER R   D C  LQGF + HS GGGT
Sbjct: 119 IDSVLERIRRMADNCSGLQGFLVFHSFGGGT 149


>SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha
           2|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 449

 Score = 86.6 bits (205), Expect = 3e-18
 Identities = 44/110 (40%), Positives = 63/110 (57%)
 Frame = +1

Query: 256 VPRAILVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDV 435
           VPR+I VDLEP  +D VR+GP+  +F P+  + G+  A NN+A+GHYT G ELVD V D 
Sbjct: 62  VPRSIYVDLEPNVIDQVRTGPYRDLFHPEQLITGKEDASNNYARGHYTVGKELVDEVTDK 121

Query: 436 VRKEAESW*LPPGIPTDTLARRXHRSGMGTLLIFKIREEYPDRIMNTYSV 585
           +R+ A++     G            SG G LL+ ++  EY  +    +SV
Sbjct: 122 IRRIADNCSGLQGFLVFHSFGGGTGSGFGALLLERLAMEYTKKSKLQFSV 171



 Score = 46.0 bits (104), Expect = 5e-06
 Identities = 23/60 (38%), Positives = 32/60 (53%), Gaps = 2/60 (3%)
 Frame = +2

Query: 80  MREIVHIQAGQCGNQIGAKFWEIISDEHGIDPTGAYHGDSDLQLE--RINVYYNEASGGK 253
           MREI+ I  GQ G QIG   WE+   EHGI P G  + ++  Q      + +++E   GK
Sbjct: 1   MREIISIHVGQAGTQIGNACWELYCLEHGIQPNGYMNPETASQNSDGGFSTFFSETGQGK 60


>SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 446

 Score = 80.2 bits (189), Expect = 3e-16
 Identities = 37/112 (33%), Positives = 67/112 (59%), Gaps = 2/112 (1%)
 Frame = +1

Query: 256 VPRAILVDLEPGTMDSVRSGPFGQIFRPDNFVFGQS--GAGNNWAKGHYTEGAELVDSVL 429
           +PRAIL+DLEP  ++++ S  +G ++ P+N +  ++  GAGNNWA G Y+    + + ++
Sbjct: 61  IPRAILIDLEPRVVNNILSDTYGSLYNPENILITKNGGGAGNNWANG-YSHAERIFEDIM 119

Query: 430 DVVRKEAESW*LPPGIPTDTLARRXHRSGMGTLLIFKIREEYPDRIMNTYSV 585
           D++ +EA+      G            SG+G+ L+ ++ + YP +I+ TYSV
Sbjct: 120 DMIDREADGSDSLEGFSLLHSIAGGTGSGLGSFLLERLNDRYPKKIIQTYSV 171



 Score = 56.4 bits (130), Expect = 4e-09
 Identities = 23/53 (43%), Positives = 37/53 (69%)
 Frame = +2

Query: 83  REIVHIQAGQCGNQIGAKFWEIISDEHGIDPTGAYHGDSDLQLERINVYYNEA 241
           REI+ +QAGQCGNQIG++FW+ +  EHGI P G     +   ++R +V++ ++
Sbjct: 3   REIITLQAGQCGNQIGSQFWQQLCLEHGIGPDGTLESFATEGVDRKDVFFYQS 55


>SPBC19G7.16 |iws1||transcription elongation factor complex subunit
           Iws1 |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 428

 Score = 27.1 bits (57), Expect = 2.5
 Identities = 10/34 (29%), Positives = 22/34 (64%)
 Frame = +2

Query: 119 NQIGAKFWEIISDEHGIDPTGAYHGDSDLQLERI 220
           N++G    E+++++  +DPT A   + DLQ++ +
Sbjct: 133 NELGENEEEVLTEQKQLDPTLAAKKELDLQMDAV 166


>SPAC29E6.01 |pof11|SPAC30.05, mug156|F-box protein
           Pof11|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 506

 Score = 26.6 bits (56), Expect = 3.3
 Identities = 14/35 (40%), Positives = 19/35 (54%), Gaps = 1/35 (2%)
 Frame = +1

Query: 313 GPFGQIFRPDNFVFGQSGAG-NNWAKGHYTEGAEL 414
           GP+G +F P  F+F  +G    NW+   Y E A L
Sbjct: 157 GPYGTMFLPQQFIFDSNGRPLLNWSY-LYKEHAHL 190


>SPAP32A8.03c |||ubiquitin-protein ligase E3 |Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 513

 Score = 26.2 bits (55), Expect = 4.3
 Identities = 15/59 (25%), Positives = 25/59 (42%)
 Frame = +1

Query: 322 GQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKEAESW*LPPGIPTDTLAR 498
           G+ F P N          N   G Y  GA  +D ++  + ++A+    P   P D +A+
Sbjct: 323 GEPFNPANMFSNIFNLSGN--PGDYAWGARGLDDIISQLMEQAQGHNAPAPAPEDVIAK 379


>SPBC19C7.11 |||ClC chloride channel |Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 812

 Score = 25.8 bits (54), Expect = 5.7
 Identities = 13/49 (26%), Positives = 22/49 (44%), Gaps = 1/49 (2%)
 Frame = -1

Query: 495 SECVSWNPWRQSPRFRFLSNDV*N*IDEL-STLRVVSLGPVVTGAGLSE 352
           S C +W PW       +L       +  L + + V  + P+  G+G+SE
Sbjct: 164 SSCTAWKPWTYKFSLNYLIYTAFALLFVLCAAIMVRDVAPLAAGSGISE 212


>SPAC6C3.08 |||gankyrin|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 234

 Score = 25.4 bits (53), Expect = 7.6
 Identities = 11/25 (44%), Positives = 17/25 (68%)
 Frame = +1

Query: 382 AKGHYTEGAELVDSVLDVVRKEAES 456
           A+GH   G ELV +  D +RK++E+
Sbjct: 183 AEGHPDVGVELVRAGADTLRKDSEN 207


>SPAC8F11.09c |nnt1||nicotinamide N-methyltransferase Nnt1
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 255

 Score = 25.4 bits (53), Expect = 7.6
 Identities = 13/32 (40%), Positives = 16/32 (50%)
 Frame = -2

Query: 371 PAPDCPKTKLSGRKICPKGPERTESMVPGSKS 276
           P+   PK  L  R I P GPE  +  + GS S
Sbjct: 20  PSTPPPKEVLHTRVIVPNGPEEIKLRLVGSHS 51


>SPBC11C11.11c ||SPBC3B8.12|ATP-dependent DNA helicase Irc3
           |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 606

 Score = 25.0 bits (52), Expect = 10.0
 Identities = 17/39 (43%), Positives = 22/39 (56%)
 Frame = -1

Query: 420 IDELSTLRVVSLGPVVTGAGLSEDEVVRTEDLSERSRAD 304
           +D   +LR VSL    T AGLS DEV    + S+ S A+
Sbjct: 369 LDFCDSLRRVSLHVDPTLAGLSPDEVENFYNKSKNSLAN 407


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,551,737
Number of Sequences: 5004
Number of extensions: 48717
Number of successful extensions: 142
Number of sequences better than 10.0: 11
Number of HSP's better than 10.0 without gapping: 125
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 134
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 309878492
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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