Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= NV060428.seq
(668 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces... 256 2e-69
SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosacchar... 133 2e-32
SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|... 132 6e-32
SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|ch... 131 8e-32
SPAP32A8.03c |||ubiquitin-protein ligase E3 |Schizosaccharomyces... 28 1.1
SPCC550.14 |||vigilin |Schizosaccharomyces pombe|chr 3|||Manual 28 1.1
SPBC19G7.16 |iws1||transcription elongation factor complex subun... 27 2.4
SPBC4C3.12 |sep1||fork head transcription factor Sep1|Schizosacc... 27 3.2
SPAC29E6.01 |pof11|SPAC30.05, mug156|F-box protein Pof11|Schizos... 26 5.6
SPAC6C3.08 |||gankyrin|Schizosaccharomyces pombe|chr 1|||Manual 25 7.5
SPBC646.12c |gap1|src1, sar1|GTPase activating protein Gap1|Schi... 25 7.5
SPBC660.16 |||phosphogluconate dehydrogenase, decarboxylating |S... 25 9.9
SPAPB18E9.04c |||sequence orphan|Schizosaccharomyces pombe|chr 1... 25 9.9
>SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 448
Score = 256 bits (628), Expect = 2e-69
Identities = 116/165 (70%), Positives = 136/165 (82%)
Frame = +2
Query: 80 MREIVHIQAGQCGNQIGAKFWEIISDEHGIDPTGAYHGDSDLQLERINVYYNEASGGQDV 259
MREIVHIQAGQCGNQ+GA FW I+DEHG+D G YHG S+ Q ER+NVY+NEA+GG+ V
Sbjct: 1 MREIVHIQAGQCGNQVGAAFWSTIADEHGLDSAGIYHGTSEAQHERLNVYFNEAAGGKYV 60
Query: 260 PRAILVDLEPGTMDSVRSGPFGQIFRPDNFVFGQXGAGNNWAKGHYTEGAELVDSVLDVV 439
PRA+LVDLEPGTMD+V+SG FG +FRPDN ++GQ GAGN WAKGHYTEGAEL D+VLDVV
Sbjct: 61 PRAVLVDLEPGTMDAVKSGKFGNLFRPDNIIYGQSGAGNIWAKGHYTEGAELADAVLDVV 120
Query: 440 RKEAESCDLPPGIPTDTLARAGGPVSGMGXLLISKIREEYPDRIM 574
R+EAE+CD G T + GG SGMG LL+SKIREEYPDR+M
Sbjct: 121 RREAEACDALQGFQL-THSLGGGTGSGMGTLLLSKIREEYPDRMM 164
Score = 32.3 bits (70), Expect = 0.065
Identities = 26/67 (38%), Positives = 29/67 (43%)
Frame = +3
Query: 468 LQGFQLTHSLGRAARFPVWXXXXXXXXXXXTLTE**NTYSVVPSPKVSDTVSRTVQRHTF 647
LQGFQLTHSLG + T+SV P+PK SDTV T
Sbjct: 130 LQGFQLTHSLGGGTGSGMGTLLLSKIREEYP-DRMMATFSVAPAPKSSDTVVEPYNA-TL 187
Query: 648 SFINLVE 668
S LVE
Sbjct: 188 SMHQLVE 194
>SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha
2|Schizosaccharomyces pombe|chr 2|||Manual
Length = 449
Score = 133 bits (322), Expect = 2e-32
Identities = 67/162 (41%), Positives = 95/162 (58%), Gaps = 2/162 (1%)
Frame = +2
Query: 80 MREIVHIQAGQCGNQIGAKFWEIISDEHGIDPTGAYHGDSDLQLER--INVYYNEASGGQ 253
MREI+ I GQ G QIG WE+ EHGI P G + ++ Q + +++E G+
Sbjct: 1 MREIISIHVGQAGTQIGNACWELYCLEHGIQPNGYMNPETASQNSDGGFSTFFSETGQGK 60
Query: 254 DVPRAILVDLEPGTMDSVRSGPFGQIFRPDNFVFGQXGAGNNWAKGHYTEGAELVDSVLD 433
VPR+I VDLEP +D VR+GP+ +F P+ + G+ A NN+A+GHYT G ELVD V D
Sbjct: 61 YVPRSIYVDLEPNVIDQVRTGPYRDLFHPEQLITGKEDASNNYARGHYTVGKELVDEVTD 120
Query: 434 VVRKEAESCDLPPGIPTDTLARAGGPVSGMGXLLISKIREEY 559
+R+ A++C G + GG SG G LL+ ++ EY
Sbjct: 121 KIRRIADNCSGLQGFLV-FHSFGGGTGSGFGALLLERLAMEY 161
>SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|chr
2|||Manual
Length = 446
Score = 132 bits (318), Expect = 6e-32
Identities = 60/167 (35%), Positives = 105/167 (62%), Gaps = 2/167 (1%)
Frame = +2
Query: 83 REIVHIQAGQCGNQIGAKFWEIISDEHGIDPTGAYHGDSDLQLERINVYYNEASGGQDVP 262
REI+ +QAGQCGNQIG++FW+ + EHGI P G + ++R +V++ ++ + +P
Sbjct: 3 REIITLQAGQCGNQIGSQFWQQLCLEHGIGPDGTLESFATEGVDRKDVFFYQSDDTRYIP 62
Query: 263 RAILVDLEPGTMDSVRSGPFGQIFRPDNFVFGQ--XGAGNNWAKGHYTEGAELVDSVLDV 436
RAIL+DLEP ++++ S +G ++ P+N + + GAGNNWA G Y+ + + ++D+
Sbjct: 63 RAILIDLEPRVVNNILSDTYGSLYNPENILITKNGGGAGNNWANG-YSHAERIFEDIMDM 121
Query: 437 VRKEAESCDLPPGIPTDTLARAGGPVSGMGXLLISKIREEYPDRIMK 577
+ +EA+ D G + AGG SG+G L+ ++ + YP +I++
Sbjct: 122 IDREADGSDSLEGFSL-LHSIAGGTGSGLGSFLLERLNDRYPKKIIQ 167
>SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|chr
2|||Manual
Length = 455
Score = 131 bits (317), Expect = 8e-32
Identities = 65/166 (39%), Positives = 97/166 (58%), Gaps = 6/166 (3%)
Frame = +2
Query: 80 MREIVHIQAGQCGNQIGAKFWEIISDEHGIDPTG------AYHGDSDLQLERINVYYNEA 241
MRE++ + GQ G QIG WE+ EHGI P G H ++ + +++E
Sbjct: 1 MREVISVHVGQAGVQIGNACWELYCLEHGIGPDGFPTENSEVHKNNSYLNDGFGTFFSET 60
Query: 242 SGGQDVPRAILVDLEPGTMDSVRSGPFGQIFRPDNFVFGQXGAGNNWAKGHYTEGAELVD 421
G+ VPR+I VDLEP +D VR+GP+ +F P+ V G+ A NN+A+GHYT G E++D
Sbjct: 61 GQGKFVPRSIYVDLEPNVIDQVRTGPYKDLFHPEQMVTGKEDASNNYARGHYTVGKEMID 120
Query: 422 SVLDVVRKEAESCDLPPGIPTDTLARAGGPVSGMGXLLISKIREEY 559
SVL+ +R+ A++C G + GG SG+G LL+ ++ EY
Sbjct: 121 SVLERIRRMADNCSGLQGFLV-FHSFGGGTGSGLGALLLERLNMEY 165
>SPAP32A8.03c |||ubiquitin-protein ligase E3 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 513
Score = 28.3 bits (60), Expect = 1.1
Identities = 15/59 (25%), Positives = 26/59 (44%)
Frame = +2
Query: 323 GQIFRPDNFVFGQXGAGNNWAKGHYTEGAELVDSVLDVVRKEAESCDLPPGIPTDTLAR 499
G+ F P N N G Y GA +D ++ + ++A+ + P P D +A+
Sbjct: 323 GEPFNPANMFSNIFNLSGN--PGDYAWGARGLDDIISQLMEQAQGHNAPAPAPEDVIAK 379
>SPCC550.14 |||vigilin |Schizosaccharomyces pombe|chr 3|||Manual
Length = 1279
Score = 28.3 bits (60), Expect = 1.1
Identities = 18/63 (28%), Positives = 33/63 (52%), Gaps = 1/63 (1%)
Frame = +2
Query: 368 AGNNWA-KGHYTEGAELVDSVLDVVRKEAESCDLPPGIPTDTLARAGGPVSGMGXLLISK 544
+ + WA +GH + + S+ +++ E+C GIPT+ R + G G +I+K
Sbjct: 1177 SSSEWAVRGHKENVEKAIASLEKSIKQVMENCIAYLGIPTNLHRR----IIGSGGSIINK 1232
Query: 545 IRE 553
IR+
Sbjct: 1233 IRK 1235
>SPBC19G7.16 |iws1||transcription elongation factor complex subunit
Iws1 |Schizosaccharomyces pombe|chr 2|||Manual
Length = 428
Score = 27.1 bits (57), Expect = 2.4
Identities = 10/34 (29%), Positives = 22/34 (64%)
Frame = +2
Query: 119 NQIGAKFWEIISDEHGIDPTGAYHGDSDLQLERI 220
N++G E+++++ +DPT A + DLQ++ +
Sbjct: 133 NELGENEEEVLTEQKQLDPTLAAKKELDLQMDAV 166
>SPBC4C3.12 |sep1||fork head transcription factor
Sep1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 663
Score = 26.6 bits (56), Expect = 3.2
Identities = 19/57 (33%), Positives = 27/57 (47%), Gaps = 2/57 (3%)
Frame = -3
Query: 564 SGYSSLILEMRRXPIPETGP-PARASVSVGI-PGGKSHDSASFRTTSKTESTSSAPS 400
S YS L + + P+ P P AS G+ GGK ++ ASF+ S + PS
Sbjct: 21 SKYSELPIAYQEIPLQSLPPYPKVASKLKGVVAGGKENNIASFQKPSSKATRPYIPS 77
>SPAC29E6.01 |pof11|SPAC30.05, mug156|F-box protein
Pof11|Schizosaccharomyces pombe|chr 1|||Manual
Length = 506
Score = 25.8 bits (54), Expect = 5.6
Identities = 14/35 (40%), Positives = 18/35 (51%), Gaps = 1/35 (2%)
Frame = +2
Query: 314 GPFGQIFRPDNFVFGQXGAG-NNWAKGHYTEGAEL 415
GP+G +F P F+F G NW+ Y E A L
Sbjct: 157 GPYGTMFLPQQFIFDSNGRPLLNWSY-LYKEHAHL 190
>SPAC6C3.08 |||gankyrin|Schizosaccharomyces pombe|chr 1|||Manual
Length = 234
Score = 25.4 bits (53), Expect = 7.5
Identities = 11/25 (44%), Positives = 17/25 (68%)
Frame = +2
Query: 383 AKGHYTEGAELVDSVLDVVRKEAES 457
A+GH G ELV + D +RK++E+
Sbjct: 183 AEGHPDVGVELVRAGADTLRKDSEN 207
>SPBC646.12c |gap1|src1, sar1|GTPase activating protein
Gap1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 766
Score = 25.4 bits (53), Expect = 7.5
Identities = 15/46 (32%), Positives = 27/46 (58%)
Frame = -2
Query: 232 VVYIDALQLQVRVPMVSTGGVDAVLVGDDLPELSSDLVAALTSLDM 95
++Y+DA L +++ + G A V DLP L +D V++L S+ +
Sbjct: 511 ILYVDAKTLFIQLLRLLPSGHPATRVPLDLP-LIADSVSSLKSMSL 555
>SPBC660.16 |||phosphogluconate dehydrogenase, decarboxylating
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 492
Score = 25.0 bits (52), Expect = 9.9
Identities = 17/64 (26%), Positives = 26/64 (40%), Gaps = 1/64 (1%)
Frame = +2
Query: 377 NWAKGHYTEGAELVDSVLDVVRKEAESCDL-PPGIPTDTLARAGGPVSGMGXLLISKIRE 553
N AKG GA ++ + ++K L G P D L P+ G +++
Sbjct: 48 NEAKGKSIVGAHSLEEFVSKLKKPRVCILLVKAGKPVDYLIEGLAPLLEKGDIIVDGGNS 107
Query: 554 EYPD 565
YPD
Sbjct: 108 HYPD 111
>SPAPB18E9.04c |||sequence orphan|Schizosaccharomyces pombe|chr
1|||Manual
Length = 800
Score = 25.0 bits (52), Expect = 9.9
Identities = 14/39 (35%), Positives = 16/39 (41%)
Frame = -3
Query: 525 PIPETGPPARASVSVGIPGGKSHDSASFRTTSKTESTSS 409
P+P T S S+ GG S S T STSS
Sbjct: 107 PLPSTSTSCTTSTSIPPTGGSSSLSTPITPTVPPTSTSS 145
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,505,733
Number of Sequences: 5004
Number of extensions: 47116
Number of successful extensions: 154
Number of sequences better than 10.0: 13
Number of HSP's better than 10.0 without gapping: 137
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 145
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 305854096
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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