Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= NV060423.seq
(657 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC23A1.10 |ef1a-b||translation elongation factor EF-1 alpha Ef... 176 2e-45
SPCC794.09c |ef1a-a||translation elongation factor EF-1 alpha Ef... 176 2e-45
SPBC839.15c |ef1a-c||translation elongation factor EF-1 alpha Ef... 176 2e-45
SPCC584.04 |sup35|erf3|translation release factor eRF3 |Schizosa... 93 4e-20
SPBC25B2.01 ||SPBC2G5.08|elongation factor 1 alpha related prote... 83 5e-17
SPCP31B10.07 |eft202||translation elongation factor 2 |Schizosac... 36 0.007
SPAC513.01c |eft201|eft2-1, etf2, SPAPYUK71.04c|translation elon... 36 0.007
SPBC9B6.04c |tuf1||mitochondrial translation elongation factor E... 35 0.009
SPBC1306.01c ||SPBC409.22c|translation elongation factor G|Schiz... 29 0.59
SPBC660.10 |||translation elongation factor G|Schizosaccharomyce... 29 0.78
SPBC2G2.11 |||N-myristoyltransferase 1|Schizosaccharomyces pombe... 26 4.2
SPAC140.01 |sdh2||succinate dehydrogenase |Schizosaccharomyces p... 26 5.5
SPAC9E9.03 |leu2||3-isopropylmalate dehydratase Leu2 |Schizosacc... 26 5.5
SPBC1703.06 |pof10||F-box protein Pof10|Schizosaccharomyces pomb... 25 7.3
SPBC17G9.09 |tif213||translation initiation factor eIF2 gamma su... 25 9.6
SPBC1703.13c |||inorganic phosphate transporter |Schizosaccharom... 25 9.6
>SPAC23A1.10 |ef1a-b||translation elongation factor EF-1 alpha
Ef1a-b |Schizosaccharomyces pombe|chr 1|||Manual
Length = 460
Score = 176 bits (429), Expect = 2e-45
Identities = 103/186 (55%), Positives = 121/186 (65%), Gaps = 7/186 (3%)
Frame = +1
Query: 52 MGKEKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVL 231
MGKEK HIN+VVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEA E+GKGSFKYAWVL
Sbjct: 1 MGKEKGHINVVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEATELGKGSFKYAWVL 60
Query: 232 DKLKAEREXVSQSILLSGSSKLASTMLPSLXLLDT---EISFKNMXXXTSQG*LRCAHS* 402
DKLKAERE + I + + T ++ ++D KNM TSQ CA
Sbjct: 61 DKLKAERE---RGITIDIALWKFETPKYNVTVIDAPGHRDFIKNMITGTSQA--DCAILI 115
Query: 403 LPVXV-NFEAGISKNGQTREHALLAFTLGVK---TAHRRINXNGXSHXNHHTVSPDLREI 570
+ FEAGISK+GQTREHALLA+TLGVK A +++ G S + +
Sbjct: 116 IGGGTGEFEAGISKDGQTREHALLAYTLGVKQLIVAVNKMDTTGWSQARFEEIVKETSNF 175
Query: 571 KKEVSF 588
K+V F
Sbjct: 176 IKKVGF 181
>SPCC794.09c |ef1a-a||translation elongation factor EF-1 alpha
Ef1a-a |Schizosaccharomyces pombe|chr 3|||Manual
Length = 460
Score = 176 bits (429), Expect = 2e-45
Identities = 103/186 (55%), Positives = 121/186 (65%), Gaps = 7/186 (3%)
Frame = +1
Query: 52 MGKEKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVL 231
MGKEK HIN+VVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEA E+GKGSFKYAWVL
Sbjct: 1 MGKEKGHINVVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEATELGKGSFKYAWVL 60
Query: 232 DKLKAEREXVSQSILLSGSSKLASTMLPSLXLLDT---EISFKNMXXXTSQG*LRCAHS* 402
DKLKAERE + I + + T ++ ++D KNM TSQ CA
Sbjct: 61 DKLKAERE---RGITIDIALWKFETPKYNVTVIDAPGHRDFIKNMITGTSQA--DCAVLI 115
Query: 403 LPVXV-NFEAGISKNGQTREHALLAFTLGVK---TAHRRINXNGXSHXNHHTVSPDLREI 570
+ FEAGISK+GQTREHALLA+TLGVK A +++ G S + +
Sbjct: 116 IGGGTGEFEAGISKDGQTREHALLAYTLGVKQLIVAVNKMDTTGWSQARFEEIVKETSNF 175
Query: 571 KKEVSF 588
K+V F
Sbjct: 176 IKKVGF 181
>SPBC839.15c |ef1a-c||translation elongation factor EF-1 alpha
Ef1a-c |Schizosaccharomyces pombe|chr 2|||Manual
Length = 460
Score = 176 bits (429), Expect = 2e-45
Identities = 103/186 (55%), Positives = 121/186 (65%), Gaps = 7/186 (3%)
Frame = +1
Query: 52 MGKEKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVL 231
MGKEK HIN+VVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEA E+GKGSFKYAWVL
Sbjct: 1 MGKEKGHINVVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEATELGKGSFKYAWVL 60
Query: 232 DKLKAEREXVSQSILLSGSSKLASTMLPSLXLLDT---EISFKNMXXXTSQG*LRCAHS* 402
DKLKAERE + I + + T ++ ++D KNM TSQ CA
Sbjct: 61 DKLKAERE---RGITIDIALWKFETPKYNVTVIDAPGHRDFIKNMITGTSQA--DCAILI 115
Query: 403 LPVXV-NFEAGISKNGQTREHALLAFTLGVK---TAHRRINXNGXSHXNHHTVSPDLREI 570
+ FEAGISK+GQTREHALLA+TLGVK A +++ G S + +
Sbjct: 116 IGGGTGEFEAGISKDGQTREHALLAYTLGVKQLIVAVNKMDTTGWSQARFEEIVKETSNF 175
Query: 571 KKEVSF 588
K+V F
Sbjct: 176 IKKVGF 181
>SPCC584.04 |sup35|erf3|translation release factor eRF3
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 662
Score = 92.7 bits (220), Expect = 4e-20
Identities = 57/145 (39%), Positives = 76/145 (52%), Gaps = 3/145 (2%)
Frame = +1
Query: 64 KTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVLDKLK 243
K H+NIV IGHVD+GKST G++++ G +DKRT+EK E+EA+E GK S+ +W LD
Sbjct: 236 KEHVNIVFIGHVDAGKSTLGGNILFLTGMVDKRTMEKIEREAKEAGKESWYLSWALDSTS 295
Query: 244 AEREXVSQSILLSGSSKLASTMLPSLXLLDTEISFKNMXXXTSQG*LRCAHS*LPVXV-- 417
ERE + + T LLD K G + L +
Sbjct: 296 EERE---KGKTVEVGRAYFETEHRRFSLLDAP-GHKGYVTNMINGASQADIGVLVISARR 351
Query: 418 -NFEAGISKNGQTREHALLAFTLGV 489
FEAG + GQTREHA+LA T G+
Sbjct: 352 GEFEAGFERGGQTREHAVLARTQGI 376
>SPBC25B2.01 ||SPBC2G5.08|elongation factor 1 alpha related
protein|Schizosaccharomyces pombe|chr 2|||Manual
Length = 592
Score = 82.6 bits (195), Expect = 5e-17
Identities = 51/145 (35%), Positives = 76/145 (52%), Gaps = 3/145 (2%)
Frame = +1
Query: 64 KTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVLDKLK 243
K +++VV GHVDSGKST G ++++ G I+ R+++K EA GKGSF YAW+LD +
Sbjct: 175 KPVVHLVVTGHVDSGKSTMLGRIMFELGEINSRSMQKLHNEAANSGKGSFSYAWLLDTTE 234
Query: 244 AEREXVSQSILLSGSSKLASTMLPSLXLLDTEISFKNMXXXTSQG*LRCAHS*LPVXV-- 417
ER ++ + + +S + + D ++ G + L V
Sbjct: 235 EER---ARGVTMDVASTTFESDKKIYEIGDAP-GHRDFISGMIAGASSADFAVLVVDSSQ 290
Query: 418 -NFEAGISKNGQTREHALLAFTLGV 489
NFE G +NGQTREHA L LG+
Sbjct: 291 NNFERGFLENGQTREHAYLLRALGI 315
Score = 29.9 bits (64), Expect = 0.34
Identities = 13/28 (46%), Positives = 17/28 (60%)
Frame = +3
Query: 261 ITIDIALWKFETSXYXVTIIXAPGHRDF 344
+T+D+A FE+ I APGHRDF
Sbjct: 241 VTMDVASTTFESDKKIYEIGDAPGHRDF 268
>SPCP31B10.07 |eft202||translation elongation factor 2
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 842
Score = 35.5 bits (78), Expect = 0.007
Identities = 17/32 (53%), Positives = 19/32 (59%)
Frame = +1
Query: 52 MGKEKTHINIVVIGHVDSGKSTTTGHLIYKCG 147
MGK N+ VI HVD GKST T L+ K G
Sbjct: 13 MGKPSNVRNMSVIAHVDHGKSTLTDSLVQKAG 44
Score = 26.2 bits (55), Expect = 4.2
Identities = 10/24 (41%), Positives = 16/24 (66%)
Frame = +2
Query: 335 QRFXSRTXSXGPLRADCAVLIRSC 406
Q++ + T GP+ +CAV IR+C
Sbjct: 349 QQYRAETLYEGPMDDECAVGIRNC 372
>SPAC513.01c |eft201|eft2-1, etf2, SPAPYUK71.04c|translation
elongation factor 2 |Schizosaccharomyces pombe|chr
1|||Manual
Length = 842
Score = 35.5 bits (78), Expect = 0.007
Identities = 17/32 (53%), Positives = 19/32 (59%)
Frame = +1
Query: 52 MGKEKTHINIVVIGHVDSGKSTTTGHLIYKCG 147
MGK N+ VI HVD GKST T L+ K G
Sbjct: 13 MGKPSNVRNMSVIAHVDHGKSTLTDSLVQKAG 44
Score = 26.2 bits (55), Expect = 4.2
Identities = 10/24 (41%), Positives = 16/24 (66%)
Frame = +2
Query: 335 QRFXSRTXSXGPLRADCAVLIRSC 406
Q++ + T GP+ +CAV IR+C
Sbjct: 349 QQYRAETLYEGPMDDECAVGIRNC 372
>SPBC9B6.04c |tuf1||mitochondrial translation elongation factor
EF-Tu Tuf1 |Schizosaccharomyces pombe|chr 2|||Manual
Length = 439
Score = 35.1 bits (77), Expect = 0.009
Identities = 16/29 (55%), Positives = 20/29 (68%)
Frame = +1
Query: 58 KEKTHINIVVIGHVDSGKSTTTGHLIYKC 144
++K H+NI IGHVD GK+T T I KC
Sbjct: 49 RKKPHVNIGTIGHVDHGKTTLTA-AITKC 76
Score = 26.2 bits (55), Expect = 4.2
Identities = 18/48 (37%), Positives = 23/48 (47%), Gaps = 1/48 (2%)
Frame = +1
Query: 448 QTREHALLAFTLGVKTAHRRINXNGXSHXNH-HTVSPDLREIKKEVSF 588
QTREH LLA +GVK IN + V ++RE+ E F
Sbjct: 156 QTREHLLLARQVGVKQIVVYINKVDMVEPDMIELVEMEMRELLSEYGF 203
>SPBC1306.01c ||SPBC409.22c|translation elongation factor
G|Schizosaccharomyces pombe|chr 2|||Manual
Length = 770
Score = 29.1 bits (62), Expect = 0.59
Identities = 12/26 (46%), Positives = 17/26 (65%)
Frame = +1
Query: 76 NIVVIGHVDSGKSTTTGHLIYKCGGI 153
NI + H+DSGK+T T ++Y G I
Sbjct: 61 NIGISAHIDSGKTTFTERVLYYTGRI 86
>SPBC660.10 |||translation elongation factor G|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 813
Score = 28.7 bits (61), Expect = 0.78
Identities = 10/24 (41%), Positives = 17/24 (70%)
Frame = +1
Query: 76 NIVVIGHVDSGKSTTTGHLIYKCG 147
N+ +I H+D+GK+T T ++Y G
Sbjct: 30 NVGIIAHIDAGKTTLTEKMLYYGG 53
Score = 25.0 bits (52), Expect = 9.6
Identities = 12/31 (38%), Positives = 15/31 (48%)
Frame = +3
Query: 261 ITIDIALWKFETSXYXVTIIXAPGHRDFXQE 353
ITI+ A F + +I PGH DF E
Sbjct: 79 ITINSAAISFTWRNQRINLIDTPGHADFTFE 109
>SPBC2G2.11 |||N-myristoyltransferase 1|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 466
Score = 26.2 bits (55), Expect = 4.2
Identities = 13/34 (38%), Positives = 21/34 (61%)
Frame = +1
Query: 556 DLREIKKEVSFIHQEDWATTQLLFAFVPHFLGWA 657
+L E+ + ++ + ED AT L FA++ FL WA
Sbjct: 104 ELSEVHELLTENYVED-ATAMLRFAYISEFLRWA 136
>SPAC140.01 |sdh2||succinate dehydrogenase |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 252
Score = 25.8 bits (54), Expect = 5.5
Identities = 7/11 (63%), Positives = 9/11 (81%)
Frame = +2
Query: 617 SCCSLSCPIFW 649
+CCS SCP +W
Sbjct: 169 ACCSTSCPSYW 179
>SPAC9E9.03 |leu2||3-isopropylmalate dehydratase Leu2
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 758
Score = 25.8 bits (54), Expect = 5.5
Identities = 17/49 (34%), Positives = 28/49 (57%), Gaps = 2/49 (4%)
Frame = +1
Query: 484 GVKTAHRRINXNGXSHXN-HHTVSPDLREIKKEV-SFIHQEDWATTQLL 624
G++TA R++ + H + D R+ K++ SFIHQ D + TQ+L
Sbjct: 49 GLRTAGRKVRHPELALATVDHNIPTDPRKDMKDIASFIHQPD-SRTQVL 96
>SPBC1703.06 |pof10||F-box protein Pof10|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 662
Score = 25.4 bits (53), Expect = 7.3
Identities = 15/43 (34%), Positives = 19/43 (44%)
Frame = -3
Query: 238 VCPIPKHI*RILYPFPGPPSRTSRWYVCQYHHICRSSDQWWWT 110
V +PK I I++ F P S S C+Y S D W T
Sbjct: 31 VLNLPKEILIIIFSFLDPRSLLSAQCTCKYWKKLLSDDLSWRT 73
>SPBC17G9.09 |tif213||translation initiation factor eIF2 gamma
subunit|Schizosaccharomyces pombe|chr 2|||Manual
Length = 446
Score = 25.0 bits (52), Expect = 9.6
Identities = 12/24 (50%), Positives = 14/24 (58%)
Frame = +1
Query: 46 PKMGKEKTHINIVVIGHVDSGKST 117
P + + INI IGHV GKST
Sbjct: 15 PAIISRQATINIGTIGHVAHGKST 38
>SPBC1703.13c |||inorganic phosphate transporter
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 311
Score = 25.0 bits (52), Expect = 9.6
Identities = 8/19 (42%), Positives = 12/19 (63%)
Frame = -3
Query: 193 PGPPSRTSRWYVCQYHHIC 137
P PP +T + Y QY+ +C
Sbjct: 8 PAPPKKTLQLYTPQYYGLC 26
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,439,556
Number of Sequences: 5004
Number of extensions: 43145
Number of successful extensions: 141
Number of sequences better than 10.0: 16
Number of HSP's better than 10.0 without gapping: 126
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 135
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 297805304
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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