Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= NV060423.seq
(657 letters)
Database: bee
438 sequences; 146,343 total letters
Searching......................................................done
Score E
Sequences producing significant alignments: (bits) Value
X52884-1|CAA37066.1| 461|Apis mellifera elongation factor 1 alp... 192 2e-51
AF015267-1|AAC38959.1| 461|Apis mellifera elongation factor-1al... 189 2e-50
AY208278-1|AAO48970.1| 274|Apis mellifera elongation factor 1-a... 76 3e-16
EF013389-1|ABK54743.1| 172|Apis mellifera elongation factor 1-a... 71 1e-14
DQ667192-1|ABG75744.1| 489|Apis mellifera pH-sensitive chloride... 25 0.48
DQ667191-1|ABG75743.1| 475|Apis mellifera pH-sensitive chloride... 25 0.48
DQ667190-1|ABG75742.1| 509|Apis mellifera pH-sensitive chloride... 25 0.48
DQ667189-1|ABG75741.1| 458|Apis mellifera pH-sensitive chloride... 25 0.48
AF069739-1|AAC63272.2| 690|Apis mellifera translation initiatio... 25 0.48
AM050259-1|CAJ18340.1| 683|Apis mellifera putative H3K9 methylt... 24 1.1
DQ244074-1|ABB36784.1| 517|Apis mellifera cytochrome P450 monoo... 24 1.5
EF625897-1|ABR45904.1| 684|Apis mellifera hexamerin protein. 22 5.9
EF591128-1|ABQ59246.1| 684|Apis mellifera hexamerin 70a protein. 22 5.9
AY268031-1|AAP23056.1| 810|Apis mellifera dorsal protein splice... 21 7.8
AY268030-1|AAP23055.1| 602|Apis mellifera dorsal protein protein. 21 7.8
AB194707-1|BAD69622.1| 247|Apis mellifera heme oxygenase protein. 21 7.8
>X52884-1|CAA37066.1| 461|Apis mellifera elongation factor 1 alpha
protein.
Length = 461
Score = 192 bits (469), Expect = 2e-51
Identities = 116/203 (57%), Positives = 129/203 (63%), Gaps = 2/203 (0%)
Frame = +1
Query: 52 MGKEKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVL 231
MGKEK HINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVL
Sbjct: 1 MGKEKIHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVL 60
Query: 232 DKLKAEREXVSQSILLSGSSKLASTMLPSLXLLDTEISFKNMXXXTSQG*LRCAHS*LPV 411
DKLKAERE + + A + + KNM TSQ CA +
Sbjct: 61 DKLKAERERGITIDIALWKFETAKYYVTIIDAPGHRDFIKNMITGTSQA--DCAVLIVAA 118
Query: 412 XV-NFEAGISKNGQTREHALLAFTLGVKTAHRRINXNGXSHXNHHTVSPDLREIKKEV-S 585
+ FEAGISKNGQTREHALLAFTLGVK +N + + EIKKEV S
Sbjct: 119 GIGEFEAGISKNGQTREHALLAFTLGVKQLIVGVNKMDMTDPPYSEAR--FEEIKKEVSS 176
Query: 586 FIHQEDWATTQLLFAFVPHFLGW 654
+I + + T + AFVP GW
Sbjct: 177 YIKKIGYNTASV--AFVP-ISGW 196
>AF015267-1|AAC38959.1| 461|Apis mellifera elongation factor-1alpha
F2 protein.
Length = 461
Score = 189 bits (461), Expect = 2e-50
Identities = 118/206 (57%), Positives = 133/206 (64%), Gaps = 5/206 (2%)
Frame = +1
Query: 52 MGKEKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVL 231
MGKEK HINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVL
Sbjct: 1 MGKEKIHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVL 60
Query: 232 DKLKAERE---XVSQSILLSGSSKLASTMLPSLXLLDTEISFKNMXXXTSQG*LRCAHS* 402
DKLKAERE + ++ +SK T++ + D KNM TSQ CA
Sbjct: 61 DKLKAERERGITIDIALWKFETSKYYVTIIDAPGHRD---FIKNMITGTSQA--DCAVLI 115
Query: 403 LPVXV-NFEAGISKNGQTREHALLAFTLGVKTAHRRINXNGXSHXNHHTVSPDLREIKKE 579
+ FEAGISKNGQTREHALLAFTLGVK +N + + EIKKE
Sbjct: 116 VAAGTGEFEAGISKNGQTREHALLAFTLGVKQLIVGVNKMDSTEPPYSETR--FEEIKKE 173
Query: 580 V-SFIHQEDWATTQLLFAFVPHFLGW 654
V S+I + + + AFVP GW
Sbjct: 174 VSSYIKKIGYNPAAV--AFVP-ISGW 196
Score = 29.5 bits (63), Expect = 0.030
Identities = 33/103 (32%), Positives = 46/103 (44%), Gaps = 6/103 (5%)
Frame = +2
Query: 365 GPLRADCAVLIRSCRXX*----ISKXGSLRTVKPVSMPCSLSPSVSKQLIVG*TKMGF-- 526
G +ADCAVLI + ISK G R ++ + KQLIVG KM
Sbjct: 105 GTSQADCAVLIVAAGTGEFEAGISKNGQTREHALLAFTLGV-----KQLIVGVNKMDSTE 159
Query: 527 PTXTTIQ*AQI*GKSRRKYPSYIKKIGLQPSCCSLSCPIFWXG 655
P + + +I +++ SYIKKIG P+ + W G
Sbjct: 160 PPYSETRFEEI----KKEVSSYIKKIGYNPAAVAFVPISGWHG 198
>AY208278-1|AAO48970.1| 274|Apis mellifera elongation factor
1-alpha protein.
Length = 274
Score = 75.8 bits (178), Expect = 3e-16
Identities = 57/131 (43%), Positives = 69/131 (52%), Gaps = 1/131 (0%)
Frame = +3
Query: 261 ITIDIALWKFETSXYXVTIIXAPGHRDFXQEHXXXDLSGLIALCSFVAAGXXEFRS-XDL 437
ITIDIALWKFETS Y VTII APGHRDF + A+ VAAG EF +
Sbjct: 14 ITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVL-IVAAGTGEFEAGISK 72
Query: 438 *ERSNP*ACLARFHPRCQNSSS*DKXKWXFPLEPPYSEPRFEGNQEGSILHTSRRLGYNP 617
++ A LA Q +K EPPYSE RFE ++ + +++GYNP
Sbjct: 73 NGQTREHALLAFTLGVKQLIVGVNKMD---STEPPYSETRFEEIKK-EVSSYIKKIGYNP 128
Query: 618 AAVRFRAPFSG 650
AAV F P SG
Sbjct: 129 AAVAF-VPISG 138
Score = 71.3 bits (167), Expect = 7e-15
Identities = 62/149 (41%), Positives = 77/149 (51%), Gaps = 5/149 (3%)
Frame = +1
Query: 223 WVLDKLKAEREX---VSQSILLSGSSKLASTMLPSLXLLDTEISFKNMXXXTSQG*LRCA 393
WVLDKLKAERE + ++ +SK T++ + D KNM TSQ CA
Sbjct: 1 WVLDKLKAERERGITIDIALWKFETSKYYVTIIDAPGHRDF---IKNMITGTSQA--DCA 55
Query: 394 HS*LPVXVN-FEAGISKNGQTREHALLAFTLGVKTAHRRINXNGXSHXNHHTVSPDLREI 570
+ FEAGISKNGQTREHALLAFTLGVK +N + + EI
Sbjct: 56 VLIVAAGTGEFEAGISKNGQTREHALLAFTLGVKQLIVGVNKMDSTEPPYSETR--FEEI 113
Query: 571 KKEV-SFIHQEDWATTQLLFAFVPHFLGW 654
KKEV S+I + + + AFVP GW
Sbjct: 114 KKEVSSYIKKIGYNPAAV--AFVP-ISGW 139
Score = 29.5 bits (63), Expect = 0.030
Identities = 33/103 (32%), Positives = 46/103 (44%), Gaps = 6/103 (5%)
Frame = +2
Query: 365 GPLRADCAVLIRSCRXX*----ISKXGSLRTVKPVSMPCSLSPSVSKQLIVG*TKMGF-- 526
G +ADCAVLI + ISK G R ++ + KQLIVG KM
Sbjct: 48 GTSQADCAVLIVAAGTGEFEAGISKNGQTREHALLAFTLGV-----KQLIVGVNKMDSTE 102
Query: 527 PTXTTIQ*AQI*GKSRRKYPSYIKKIGLQPSCCSLSCPIFWXG 655
P + + +I +++ SYIKKIG P+ + W G
Sbjct: 103 PPYSETRFEEI----KKEVSSYIKKIGYNPAAVAFVPISGWHG 141
>EF013389-1|ABK54743.1| 172|Apis mellifera elongation factor
1-alpha protein.
Length = 172
Score = 70.5 bits (165), Expect = 1e-14
Identities = 54/128 (42%), Positives = 66/128 (51%), Gaps = 1/128 (0%)
Frame = +3
Query: 270 DIALWKFETSXYXVTIIXAPGHRDFXQEHXXXDLSGLIALCSFVAAGXXEFRS-XDL*ER 446
DIALWKFETS Y VTII APGHRDF + A+ VAAG EF + +
Sbjct: 1 DIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVL-IVAAGTGEFEAGISKNGQ 59
Query: 447 SNP*ACLARFHPRCQNSSS*DKXKWXFPLEPPYSEPRFEGNQEGSILHTSRRLGYNPAAV 626
+ A LA Q +K EPPYSE RFE ++ + +++GYNPAAV
Sbjct: 60 TREHALLAFTLGVKQLIVGVNKMD---STEPPYSETRFEEIKK-EVSSYIKKIGYNPAAV 115
Query: 627 RFRAPFSG 650
F P SG
Sbjct: 116 AF-VPISG 122
Score = 59.3 bits (137), Expect = 3e-11
Identities = 47/104 (45%), Positives = 55/104 (52%), Gaps = 2/104 (1%)
Frame = +1
Query: 349 KNMXXXTSQG*LRCAHS*LPVXVN-FEAGISKNGQTREHALLAFTLGVKTAHRRINXNGX 525
KNM TSQ CA + FEAGISKNGQTREHALLAFTLGVK +N
Sbjct: 27 KNMITGTSQA--DCAVLIVAAGTGEFEAGISKNGQTREHALLAFTLGVKQLIVGVNKMDS 84
Query: 526 SHXNHHTVSPDLREIKKEV-SFIHQEDWATTQLLFAFVPHFLGW 654
+ + EIKKEV S+I + + + AFVP GW
Sbjct: 85 TEPPYSETR--FEEIKKEVSSYIKKIGYNPAAV--AFVP-ISGW 123
Score = 29.5 bits (63), Expect = 0.030
Identities = 33/103 (32%), Positives = 46/103 (44%), Gaps = 6/103 (5%)
Frame = +2
Query: 365 GPLRADCAVLIRSCRXX*----ISKXGSLRTVKPVSMPCSLSPSVSKQLIVG*TKMGF-- 526
G +ADCAVLI + ISK G R ++ + KQLIVG KM
Sbjct: 32 GTSQADCAVLIVAAGTGEFEAGISKNGQTREHALLAFTLGV-----KQLIVGVNKMDSTE 86
Query: 527 PTXTTIQ*AQI*GKSRRKYPSYIKKIGLQPSCCSLSCPIFWXG 655
P + + +I +++ SYIKKIG P+ + W G
Sbjct: 87 PPYSETRFEEI----KKEVSSYIKKIGYNPAAVAFVPISGWHG 125
>DQ667192-1|ABG75744.1| 489|Apis mellifera pH-sensitive chloride
channel variant 4 protein.
Length = 489
Score = 25.4 bits (53), Expect = 0.48
Identities = 10/27 (37%), Positives = 16/27 (59%)
Frame = +1
Query: 550 SPDLREIKKEVSFIHQEDWATTQLLFA 630
SPD +K EV F+ Q+ W +L ++
Sbjct: 99 SPDESSLKYEVEFLLQQQWYDPRLRYS 125
>DQ667191-1|ABG75743.1| 475|Apis mellifera pH-sensitive chloride
channel variant 3 protein.
Length = 475
Score = 25.4 bits (53), Expect = 0.48
Identities = 10/27 (37%), Positives = 16/27 (59%)
Frame = +1
Query: 550 SPDLREIKKEVSFIHQEDWATTQLLFA 630
SPD +K EV F+ Q+ W +L ++
Sbjct: 99 SPDESSLKYEVEFLLQQQWYDPRLRYS 125
>DQ667190-1|ABG75742.1| 509|Apis mellifera pH-sensitive chloride
channel variant 1 protein.
Length = 509
Score = 25.4 bits (53), Expect = 0.48
Identities = 10/27 (37%), Positives = 16/27 (59%)
Frame = +1
Query: 550 SPDLREIKKEVSFIHQEDWATTQLLFA 630
SPD +K EV F+ Q+ W +L ++
Sbjct: 150 SPDESSLKYEVEFLLQQQWYDPRLRYS 176
>DQ667189-1|ABG75741.1| 458|Apis mellifera pH-sensitive chloride
channel protein.
Length = 458
Score = 25.4 bits (53), Expect = 0.48
Identities = 10/27 (37%), Positives = 16/27 (59%)
Frame = +1
Query: 550 SPDLREIKKEVSFIHQEDWATTQLLFA 630
SPD +K EV F+ Q+ W +L ++
Sbjct: 99 SPDESSLKYEVEFLLQQQWYDPRLRYS 125
>AF069739-1|AAC63272.2| 690|Apis mellifera translation initiation
factor 2 protein.
Length = 690
Score = 25.4 bits (53), Expect = 0.48
Identities = 9/18 (50%), Positives = 13/18 (72%)
Frame = +1
Query: 64 KTHINIVVIGHVDSGKST 117
K H + ++GHVD GK+T
Sbjct: 143 KRHPIVTIMGHVDHGKTT 160
>AM050259-1|CAJ18340.1| 683|Apis mellifera putative H3K9
methyltransferase protein.
Length = 683
Score = 24.2 bits (50), Expect = 1.1
Identities = 11/15 (73%), Positives = 11/15 (73%)
Frame = +1
Query: 73 INIVVIGHVDSGKST 117
INI IGHV GKST
Sbjct: 43 INIGTIGHVAHGKST 57
>DQ244074-1|ABB36784.1| 517|Apis mellifera cytochrome P450
monooxygenase protein.
Length = 517
Score = 23.8 bits (49), Expect = 1.5
Identities = 11/20 (55%), Positives = 14/20 (70%)
Frame = -2
Query: 65 FSLPIFG*SRITNCV*YPEL 6
FSLPIFG I +C+ Y +L
Sbjct: 57 FSLPIFGTRWIFSCIGYYKL 76
>EF625897-1|ABR45904.1| 684|Apis mellifera hexamerin protein.
Length = 684
Score = 21.8 bits (44), Expect = 5.9
Identities = 8/20 (40%), Positives = 12/20 (60%)
Frame = +3
Query: 507 DKXKWXFPLEPPYSEPRFEG 566
DK + FPL+ P + +EG
Sbjct: 643 DKRSFGFPLDKPLYDFNYEG 662
>EF591128-1|ABQ59246.1| 684|Apis mellifera hexamerin 70a protein.
Length = 684
Score = 21.8 bits (44), Expect = 5.9
Identities = 8/20 (40%), Positives = 12/20 (60%)
Frame = +3
Query: 507 DKXKWXFPLEPPYSEPRFEG 566
DK + FPL+ P + +EG
Sbjct: 643 DKRSFGFPLDKPLYDFNYEG 662
>AY268031-1|AAP23056.1| 810|Apis mellifera dorsal protein splice
variant B protein.
Length = 810
Score = 21.4 bits (43), Expect = 7.8
Identities = 8/18 (44%), Positives = 12/18 (66%)
Frame = +3
Query: 510 KXKWXFPLEPPYSEPRFE 563
K K+ PL+P S+P F+
Sbjct: 199 KRKFNVPLQPVVSDPIFD 216
>AY268030-1|AAP23055.1| 602|Apis mellifera dorsal protein protein.
Length = 602
Score = 21.4 bits (43), Expect = 7.8
Identities = 8/18 (44%), Positives = 12/18 (66%)
Frame = +3
Query: 510 KXKWXFPLEPPYSEPRFE 563
K K+ PL+P S+P F+
Sbjct: 199 KRKFNVPLQPVVSDPIFD 216
>AB194707-1|BAD69622.1| 247|Apis mellifera heme oxygenase protein.
Length = 247
Score = 21.4 bits (43), Expect = 7.8
Identities = 11/36 (30%), Positives = 16/36 (44%)
Frame = +1
Query: 541 HTVSPDLREIKKEVSFIHQEDWATTQLLFAFVPHFL 648
H++S L K F+ WA L+F V +L
Sbjct: 21 HSISDSLVNAKLAFGFLDNSVWADGLLVFYEVFRYL 56
Database: bee
Posted date: Oct 23, 2007 1:17 PM
Number of letters in database: 146,343
Number of sequences in database: 438
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 168,076
Number of Sequences: 438
Number of extensions: 2813
Number of successful extensions: 40
Number of sequences better than 10.0: 16
Number of HSP's better than 10.0 without gapping: 23
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 28
length of database: 146,343
effective HSP length: 56
effective length of database: 121,815
effective search space used: 19734030
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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