Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= NV060423.seq
(657 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g60390.1 68418.m07574 elongation factor 1-alpha / EF-1-alpha ... 165 2e-41
At1g07940.1 68414.m00863 elongation factor 1-alpha / EF-1-alpha ... 165 2e-41
At1g07930.1 68414.m00862 elongation factor 1-alpha / EF-1-alpha ... 165 2e-41
At1g07920.1 68414.m00861 elongation factor 1-alpha / EF-1-alpha ... 165 2e-41
At5g10630.1 68418.m01231 elongation factor 1-alpha, putative / E... 85 3e-17
At1g18070.1 68414.m02236 EF-1-alpha-related GTP-binding protein,... 82 4e-16
At4g20360.1 68417.m02971 elongation factor Tu / EF-Tu (TUFA) ide... 39 0.003
At4g02930.1 68417.m00399 elongation factor Tu, putative / EF-Tu,... 34 0.096
At4g34100.1 68417.m04838 zinc finger (C3HC4-type RING finger) fa... 33 0.17
At3g22980.1 68416.m02898 elongation factor Tu family protein sim... 33 0.22
At1g62750.1 68414.m07082 elongation factor Tu family protein sim... 33 0.22
At1g56070.1 68414.m06438 elongation factor 2, putative / EF-2, p... 29 2.7
At5g08650.1 68418.m01029 GTP-binding protein LepA, putative 28 4.8
At5g39900.1 68418.m04839 GTP-binding protein LepA, putative GTP-... 28 6.3
At2g45030.1 68415.m05606 mitochondrial elongation factor, putati... 28 6.3
At1g45332.1 68414.m05195 mitochondrial elongation factor, putati... 28 6.3
>At5g60390.1 68418.m07574 elongation factor 1-alpha / EF-1-alpha
identical to SWISS-PROT:P13905 elongation factor 1-alpha
(EF-1-alpha) [Arabidopsis thaliana]
Length = 449
Score = 165 bits (401), Expect = 2e-41
Identities = 95/151 (62%), Positives = 106/151 (70%), Gaps = 4/151 (2%)
Frame = +1
Query: 52 MGKEKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVL 231
MGKEK HINIVVIGHVDSGKSTTTGHLIYK GGIDKR IE+FEKEA EM K SFKYAWVL
Sbjct: 1 MGKEKFHINIVVIGHVDSGKSTTTGHLIYKLGGIDKRVIERFEKEAAEMNKRSFKYAWVL 60
Query: 232 DKLKAERE---XVSQSILLSGSSKLASTMLPSLXLLDTEISFKNMXXXTSQG*LRCAHS* 402
DKLKAERE + ++ ++K T++ + D KNM TSQ CA
Sbjct: 61 DKLKAERERGITIDIALWKFETTKYYCTVIDAPGHRD---FIKNMITGTSQA--DCAVLI 115
Query: 403 LPVXV-NFEAGISKNGQTREHALLAFTLGVK 492
+ FEAGISK+GQTREHALLAFTLGVK
Sbjct: 116 IDSTTGGFEAGISKDGQTREHALLAFTLGVK 146
>At1g07940.1 68414.m00863 elongation factor 1-alpha / EF-1-alpha
identical to GB:CAA34456 from [Arabidopsis thaliana]
(Plant Mol. Biol. 14 (1), 107-110 (1990))
Length = 449
Score = 165 bits (401), Expect = 2e-41
Identities = 95/151 (62%), Positives = 106/151 (70%), Gaps = 4/151 (2%)
Frame = +1
Query: 52 MGKEKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVL 231
MGKEK HINIVVIGHVDSGKSTTTGHLIYK GGIDKR IE+FEKEA EM K SFKYAWVL
Sbjct: 1 MGKEKFHINIVVIGHVDSGKSTTTGHLIYKLGGIDKRVIERFEKEAAEMNKRSFKYAWVL 60
Query: 232 DKLKAERE---XVSQSILLSGSSKLASTMLPSLXLLDTEISFKNMXXXTSQG*LRCAHS* 402
DKLKAERE + ++ ++K T++ + D KNM TSQ CA
Sbjct: 61 DKLKAERERGITIDIALWKFETTKYYCTVIDAPGHRD---FIKNMITGTSQA--DCAVLI 115
Query: 403 LPVXV-NFEAGISKNGQTREHALLAFTLGVK 492
+ FEAGISK+GQTREHALLAFTLGVK
Sbjct: 116 IDSTTGGFEAGISKDGQTREHALLAFTLGVK 146
>At1g07930.1 68414.m00862 elongation factor 1-alpha / EF-1-alpha
identical to GB:CAA34456 from [Arabidopsis thaliana]
(Plant Mol. Biol. 14 (1), 107-110 (1990))
Length = 449
Score = 165 bits (401), Expect = 2e-41
Identities = 95/151 (62%), Positives = 106/151 (70%), Gaps = 4/151 (2%)
Frame = +1
Query: 52 MGKEKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVL 231
MGKEK HINIVVIGHVDSGKSTTTGHLIYK GGIDKR IE+FEKEA EM K SFKYAWVL
Sbjct: 1 MGKEKFHINIVVIGHVDSGKSTTTGHLIYKLGGIDKRVIERFEKEAAEMNKRSFKYAWVL 60
Query: 232 DKLKAERE---XVSQSILLSGSSKLASTMLPSLXLLDTEISFKNMXXXTSQG*LRCAHS* 402
DKLKAERE + ++ ++K T++ + D KNM TSQ CA
Sbjct: 61 DKLKAERERGITIDIALWKFETTKYYCTVIDAPGHRD---FIKNMITGTSQA--DCAVLI 115
Query: 403 LPVXV-NFEAGISKNGQTREHALLAFTLGVK 492
+ FEAGISK+GQTREHALLAFTLGVK
Sbjct: 116 IDSTTGGFEAGISKDGQTREHALLAFTLGVK 146
>At1g07920.1 68414.m00861 elongation factor 1-alpha / EF-1-alpha
identical to GB:CAA34456 from [Arabidopsis thaliana]
(Plant Mol. Biol. 14 (1), 107-110 (1990))
Length = 449
Score = 165 bits (401), Expect = 2e-41
Identities = 95/151 (62%), Positives = 106/151 (70%), Gaps = 4/151 (2%)
Frame = +1
Query: 52 MGKEKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVL 231
MGKEK HINIVVIGHVDSGKSTTTGHLIYK GGIDKR IE+FEKEA EM K SFKYAWVL
Sbjct: 1 MGKEKFHINIVVIGHVDSGKSTTTGHLIYKLGGIDKRVIERFEKEAAEMNKRSFKYAWVL 60
Query: 232 DKLKAERE---XVSQSILLSGSSKLASTMLPSLXLLDTEISFKNMXXXTSQG*LRCAHS* 402
DKLKAERE + ++ ++K T++ + D KNM TSQ CA
Sbjct: 61 DKLKAERERGITIDIALWKFETTKYYCTVIDAPGHRD---FIKNMITGTSQA--DCAVLI 115
Query: 403 LPVXV-NFEAGISKNGQTREHALLAFTLGVK 492
+ FEAGISK+GQTREHALLAFTLGVK
Sbjct: 116 IDSTTGGFEAGISKDGQTREHALLAFTLGVK 146
>At5g10630.1 68418.m01231 elongation factor 1-alpha, putative /
EF-1-alpha, putative contains similarity to
SWISS-PROT:Q9YAV0 elongation factor 1-alpha (EF-1-alpha)
[Aeropyrum pernix]
Length = 667
Score = 85.4 bits (202), Expect = 3e-17
Identities = 54/153 (35%), Positives = 83/153 (54%), Gaps = 4/153 (2%)
Frame = +1
Query: 67 THINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVLDKLKA 246
+ +N+ ++GHVDSGKST +G L++ G I ++ + K+EKEA+ GKGSF YAW LD+
Sbjct: 238 SQLNLAIVGHVDSGKSTLSGRLLHLLGRISQKQMHKYEKEAKLQGKGSFAYAWALDESAE 297
Query: 247 EREXVSQSILLSGSSKLASTMLPSLXLLDTEISFKNMXXXTSQG*LRCAHS*LPVXVN-- 420
ERE + I ++ + ++ + LLD+ K+ G + + L + +
Sbjct: 298 ERE---RGITMTVAVAYFNSKRHHVVLLDSP-GHKDFVPNMIAGATQADAAILVIDASVG 353
Query: 421 -FEAGISK-NGQTREHALLAFTLGVKTAHRRIN 513
FEAG GQTREHA + GV+ IN
Sbjct: 354 AFEAGFDNLKGQTREHARVLRGFGVEQVIVAIN 386
>At1g18070.1 68414.m02236 EF-1-alpha-related GTP-binding protein,
putative similar to EF-1-alpha-related GTP-binding
protein gi|1009232|gb|AAA79032
Length = 532
Score = 81.8 bits (193), Expect = 4e-16
Identities = 50/143 (34%), Positives = 73/143 (51%)
Frame = +1
Query: 61 EKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVLDKL 240
+K H+N+V IGHVD+GKST G +++ G +D R I+K+EKEA++ + S+ A+++D
Sbjct: 98 KKRHLNVVFIGHVDAGKSTIGGQILFLSGQVDDRQIQKYEKEAKDKSRESWYMAYIMDTN 157
Query: 241 KAEREXVSQSILLSGSSKLASTMLPSLXLLDTEISFKNMXXXTSQG*LRCAHS*LPVXVN 420
+ ER + + ST L + NM SQ +
Sbjct: 158 EEERLKGKTVEVGRAHFETESTRFTILDAPGHKSYVPNMISGASQADIGVLVI-SARKGE 216
Query: 421 FEAGISKNGQTREHALLAFTLGV 489
FE G + GQTREH LA TLGV
Sbjct: 217 FETGYERGGQTREHVQLAKTLGV 239
Score = 27.5 bits (58), Expect = 8.3
Identities = 10/27 (37%), Positives = 16/27 (59%)
Frame = +3
Query: 264 TIDIALWKFETSXYXVTIIXAPGHRDF 344
T+++ FET TI+ APGH+ +
Sbjct: 166 TVEVGRAHFETESTRFTILDAPGHKSY 192
>At4g20360.1 68417.m02971 elongation factor Tu / EF-Tu (TUFA)
identical to SWISS-PROT:P17745 elongation factor Tu,
chloroplast precursor (EF-Tu) [Arabidopsis thaliana]
Length = 476
Score = 39.1 bits (87), Expect = 0.003
Identities = 17/45 (37%), Positives = 25/45 (55%)
Frame = +1
Query: 49 KMGKEKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEK 183
K ++K H+NI IGHVD GK+T T L I +K+++
Sbjct: 72 KFERKKPHVNIGTIGHVDHGKTTLTAALTMALASIGSSVAKKYDE 116
>At4g02930.1 68417.m00399 elongation factor Tu, putative / EF-Tu,
putative similar to mitochondrial elongation factor Tu
[Arabidopsis thaliana] gi|1149571|emb|CAA61511
Length = 454
Score = 33.9 bits (74), Expect = 0.096
Identities = 16/40 (40%), Positives = 22/40 (55%)
Frame = +1
Query: 4 TSSGYYTQFVIRD*PKMGKEKTHINIVVIGHVDSGKSTTT 123
+SS + T R + K H+N+ IGHVD GK+T T
Sbjct: 45 SSSTFGTSSFWRSMATFTRNKPHVNVGTIGHVDHGKTTLT 84
>At4g34100.1 68417.m04838 zinc finger (C3HC4-type RING finger) family
protein contains Pfam profile: PF00097 zinc finger, C3HC4
type (RING finger)
Length = 1092
Score = 33.1 bits (72), Expect = 0.17
Identities = 22/65 (33%), Positives = 33/65 (50%), Gaps = 1/65 (1%)
Frame = -1
Query: 609 SPIFLMYEGYFL-LDFPQIWAHCMVVXVGXPIXVYPTMSCFDTEGESEQGMLTGLTVLRD 433
SP+FL+Y+ + L L F +IW +++ PI + F+ E L GL VLR+
Sbjct: 907 SPVFLLYQDWALGLIFLKIWTRLVMLDHMLPIVDDSWRAKFERVREDGFSRLQGLWVLRE 966
Query: 432 PXFEI 418
F I
Sbjct: 967 IVFPI 971
>At3g22980.1 68416.m02898 elongation factor Tu family protein
similar to eukaryotic translation elongation factor 2
GB:NP_001952 [Homo sapiens]
Length = 1015
Score = 32.7 bits (71), Expect = 0.22
Identities = 13/25 (52%), Positives = 16/25 (64%)
Frame = +1
Query: 76 NIVVIGHVDSGKSTTTGHLIYKCGG 150
NI ++ HVD GK+T HLI GG
Sbjct: 11 NICILAHVDHGKTTLADHLIASSGG 35
>At1g62750.1 68414.m07082 elongation factor Tu family protein
similar to elongation factor G SP:P34811 [Glycine max
(Soybean)]
Length = 783
Score = 32.7 bits (71), Expect = 0.22
Identities = 12/28 (42%), Positives = 20/28 (71%)
Frame = +1
Query: 64 KTHINIVVIGHVDSGKSTTTGHLIYKCG 147
K + NI ++ H+D+GK+TTT ++Y G
Sbjct: 94 KDYRNIGIMAHIDAGKTTTTERILYYTG 121
>At1g56070.1 68414.m06438 elongation factor 2, putative / EF-2,
putative similar to ELONGATION FACTOR 2 GB:O14460 from
[Schizosaccharomyces pombe]
Length = 843
Score = 29.1 bits (62), Expect = 2.7
Identities = 13/24 (54%), Positives = 15/24 (62%)
Frame = +1
Query: 76 NIVVIGHVDSGKSTTTGHLIYKCG 147
N+ VI HVD GKST T L+ G
Sbjct: 21 NMSVIAHVDHGKSTLTDSLVAAAG 44
>At5g08650.1 68418.m01029 GTP-binding protein LepA, putative
Length = 681
Score = 28.3 bits (60), Expect = 4.8
Identities = 11/33 (33%), Positives = 18/33 (54%)
Frame = +1
Query: 76 NIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEK 174
N +I H+D GKST L+ G + R +++
Sbjct: 88 NFSIIAHIDHGKSTLADKLLQVTGTVQNRDMKE 120
>At5g39900.1 68418.m04839 GTP-binding protein LepA, putative
GTP-binding protein GUF1 - Saccharomyces cerevisiae,
PIR:S50374
Length = 661
Score = 27.9 bits (59), Expect = 6.3
Identities = 16/40 (40%), Positives = 19/40 (47%)
Frame = +1
Query: 40 D*PKMGKEKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDK 159
D K EK N +I H+D GKST L+ G I K
Sbjct: 57 DLTKFPSEKIR-NFSIIAHIDHGKSTLADRLMELTGTIKK 95
>At2g45030.1 68415.m05606 mitochondrial elongation factor, putative
similar to SP|P25039 Elongation factor G 1,
mitochondrial precursor (mEF-G-1) {Saccharomyces
cerevisiae}; contains Pfam profiles PF00009: Elongation
factor Tu GTP binding domain, PF03764: Elongation factor
G domain IV, PF00679: Elongation factor G C-terminus
Length = 754
Score = 27.9 bits (59), Expect = 6.3
Identities = 11/26 (42%), Positives = 17/26 (65%)
Frame = +1
Query: 76 NIVVIGHVDSGKSTTTGHLIYKCGGI 153
NI + H+DSGK+T T +++ G I
Sbjct: 67 NIGISAHIDSGKTTLTERVLFYTGRI 92
>At1g45332.1 68414.m05195 mitochondrial elongation factor, putative
similar to mitochondrial elongation factor GI:3917 from
[Saccharomyces cerevisiae]
Length = 754
Score = 27.9 bits (59), Expect = 6.3
Identities = 11/26 (42%), Positives = 17/26 (65%)
Frame = +1
Query: 76 NIVVIGHVDSGKSTTTGHLIYKCGGI 153
NI + H+DSGK+T T +++ G I
Sbjct: 67 NIGISAHIDSGKTTLTERVLFYTGRI 92
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 13,033,365
Number of Sequences: 28952
Number of extensions: 236575
Number of successful extensions: 629
Number of sequences better than 10.0: 16
Number of HSP's better than 10.0 without gapping: 602
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 619
length of database: 12,070,560
effective HSP length: 78
effective length of database: 9,812,304
effective search space used: 1373722560
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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