BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= NV060421.seq (666 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBC106.15 |idi1||isopentenyl-diphosphate delta-isomerase Idi1|S... 51 2e-07 SPAC17A2.10c |||sequence orphan|Schizosaccharomyces pombe|chr 1|... 25 7.4 SPBC1861.06c |mug131||S. pombe specific UPF0300 family protein 4... 25 9.8 SPAC13G7.05 |||acyl-coA-sterol acyltransferase |Schizosaccharomy... 25 9.8 SPBC17A3.05c |||DNAJ/DUF1977 DNAJB12 homolog|Schizosaccharomyces... 25 9.8 >SPBC106.15 |idi1||isopentenyl-diphosphate delta-isomerase Idi1|Schizosaccharomyces pombe|chr 2|||Manual Length = 227 Score = 50.8 bits (116), Expect = 2e-07 Identities = 20/44 (45%), Positives = 33/44 (75%) Frame = +3 Query: 273 TKRECHKVGPDGDVLLHRAFSVFLFNKRGDMFLQRRSSQKVTYP 404 TK+ECH + LLHRAFS+F+F+++ + LQ+R+ +K+T+P Sbjct: 36 TKKECHLMENINKGLLHRAFSMFIFDEQNRLLLQQRAEEKITFP 79 Score = 26.6 bits (56), Expect = 3.2 Identities = 9/22 (40%), Positives = 12/22 (54%) Frame = +2 Query: 374 EEIKPKSNISRFYTNACCSHPL 439 + + K +TN CCSHPL Sbjct: 70 QRAEEKITFPSLWTNTCCSHPL 91 >SPAC17A2.10c |||sequence orphan|Schizosaccharomyces pombe|chr 1|||Manual Length = 230 Score = 25.4 bits (53), Expect = 7.4 Identities = 19/57 (33%), Positives = 30/57 (52%) Frame = -2 Query: 254 SFSSTSRQISLSKASAWANSRPSLSRVAGFTGSEAKNLFSTLRVLHSDLVSFLTNIF 84 S S +++SLS + + RP + + FTGSE +LF L + L+ FL+ F Sbjct: 76 SLSINPKKLSLSFSFPLSQKRPFPNFLHPFTGSEL-SLFRCLLLFFFFLLFFLSFSF 131 >SPBC1861.06c |mug131||S. pombe specific UPF0300 family protein 4|Schizosaccharomyces pombe|chr 2|||Manual Length = 433 Score = 25.0 bits (52), Expect = 9.8 Identities = 12/33 (36%), Positives = 19/33 (57%) Frame = -2 Query: 164 TGSEAKNLFSTLRVLHSDLVSFLTNIFVCMNFT 66 T EA +L S+L H+D + L+N+ +FT Sbjct: 78 TSMEALDLSSSLTTPHADKLLKLSNLIAAKSFT 110 >SPAC13G7.05 |||acyl-coA-sterol acyltransferase |Schizosaccharomyces pombe|chr 1|||Manual Length = 537 Score = 25.0 bits (52), Expect = 9.8 Identities = 11/35 (31%), Positives = 18/35 (51%) Frame = -2 Query: 167 FTGSEAKNLFSTLRVLHSDLVSFLTNIFVCMNFTE 63 F+ S S +R+LH++ L + C+N TE Sbjct: 219 FSYSHHMGYISEIRILHNEYEKLLKFVRECLNSTE 253 >SPBC17A3.05c |||DNAJ/DUF1977 DNAJB12 homolog|Schizosaccharomyces pombe|chr 2|||Manual Length = 403 Score = 25.0 bits (52), Expect = 9.8 Identities = 20/54 (37%), Positives = 27/54 (50%), Gaps = 2/54 (3%) Frame = -3 Query: 172 QVSQVLKPK-IFSRLLGCSTVILLAF*PTFSYV-*ILLNYTFTLQYNINNNVSR 17 Q +Q + PK IF +LL VIL AF FS+ +N ++ Q N V R Sbjct: 254 QQAQDMPPKSIFYQLLPLIVVILFAFLSNFSWSDSTSVNTRYSFQQNYKYTVPR 307 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,669,707 Number of Sequences: 5004 Number of extensions: 53003 Number of successful extensions: 130 Number of sequences better than 10.0: 5 Number of HSP's better than 10.0 without gapping: 126 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 130 length of database: 2,362,478 effective HSP length: 70 effective length of database: 2,012,198 effective search space used: 303841898 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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