BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= NV060421.seq (666 letters) Database: nematostella 59,808 sequences; 16,821,457 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SB_58402| Best HMM Match : No HMM Matches (HMM E-Value=.) 61 7e-10 SB_56787| Best HMM Match : Keratin_B2 (HMM E-Value=0.34) 30 1.5 SB_35375| Best HMM Match : RVT_1 (HMM E-Value=0.00074) 29 2.6 SB_47598| Best HMM Match : No HMM Matches (HMM E-Value=.) 28 7.9 >SB_58402| Best HMM Match : No HMM Matches (HMM E-Value=.) Length = 259 Score = 61.3 bits (142), Expect = 7e-10 Identities = 29/51 (56%), Positives = 36/51 (70%), Gaps = 1/51 (1%) Frame = +3 Query: 255 NFIGTATKRECH-KVGPDGDVLLHRAFSVFLFNKRGDMFLQRRSSQKVTYP 404 N G ATKRECH K LLHRAFSVFLFN +GD+ +Q+R+ K+T+P Sbjct: 59 NVQGCATKRECHLKENIVEQGLLHRAFSVFLFNSKGDLLIQQRADTKITFP 109 Score = 35.1 bits (77), Expect = 0.052 Identities = 11/18 (61%), Positives = 16/18 (88%) Frame = +2 Query: 410 YTNACCSHPLYIDEKPEE 463 +TN+CCSHPLY++ + EE Sbjct: 112 FTNSCCSHPLYVESEMEE 129 >SB_56787| Best HMM Match : Keratin_B2 (HMM E-Value=0.34) Length = 527 Score = 30.3 bits (65), Expect = 1.5 Identities = 21/91 (23%), Positives = 42/91 (46%) Frame = -2 Query: 314 HITIRSYFMTFSLCSRSYEVSFSSTSRQISLSKASAWANSRPSLSRVAGFTGSEAKNLFS 135 H+TI S+ + ++ SRS V+ +S SR ++++ S T + N Sbjct: 337 HVTITSHHIKVTITSRSRHVTITSRSRHVTITSRSRHGTIISRSRHHDNITVTTCHN-HI 395 Query: 134 TLRVLHSDLVSFLTNIFVCMNFTELHFYITI 42 T+ H+ + S + ++ + T H +IT+ Sbjct: 396 TVTTCHNHITSHQGHNYITV--TTCHNHITV 424 >SB_35375| Best HMM Match : RVT_1 (HMM E-Value=0.00074) Length = 996 Score = 29.5 bits (63), Expect = 2.6 Identities = 12/31 (38%), Positives = 18/31 (58%) Frame = +3 Query: 63 FSKIHTYENVGQKANKITVEHPKSREKIFGF 155 F +IH G + KIT HPK++ ++ GF Sbjct: 760 FHRIHRVHREGFRHKKITPRHPKAQGQVEGF 790 >SB_47598| Best HMM Match : No HMM Matches (HMM E-Value=.) Length = 2332 Score = 27.9 bits (59), Expect = 7.9 Identities = 17/38 (44%), Positives = 19/38 (50%) Frame = -2 Query: 620 GRTVCGXSXVRPTLPSPGSX*CTRVMTVTVPGPTDLVD 507 GR VC S L SPGS C V T+ VPG + D Sbjct: 321 GRMVCICSDATVRLISPGSGAC--VTTLLVPGMQTIAD 356 Database: nematostella Posted date: Oct 22, 2007 1:22 PM Number of letters in database: 16,821,457 Number of sequences in database: 59,808 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 20,160,494 Number of Sequences: 59808 Number of extensions: 422940 Number of successful extensions: 781 Number of sequences better than 10.0: 4 Number of HSP's better than 10.0 without gapping: 717 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 780 length of database: 16,821,457 effective HSP length: 79 effective length of database: 12,096,625 effective search space used: 1717720750 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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