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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= NV060421.seq
         (666 letters)

Database: nematostella 
           59,808 sequences; 16,821,457 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SB_58402| Best HMM Match : No HMM Matches (HMM E-Value=.)              61   7e-10
SB_56787| Best HMM Match : Keratin_B2 (HMM E-Value=0.34)               30   1.5  
SB_35375| Best HMM Match : RVT_1 (HMM E-Value=0.00074)                 29   2.6  
SB_47598| Best HMM Match : No HMM Matches (HMM E-Value=.)              28   7.9  

>SB_58402| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 259

 Score = 61.3 bits (142), Expect = 7e-10
 Identities = 29/51 (56%), Positives = 36/51 (70%), Gaps = 1/51 (1%)
 Frame = +3

Query: 255 NFIGTATKRECH-KVGPDGDVLLHRAFSVFLFNKRGDMFLQRRSSQKVTYP 404
           N  G ATKRECH K       LLHRAFSVFLFN +GD+ +Q+R+  K+T+P
Sbjct: 59  NVQGCATKRECHLKENIVEQGLLHRAFSVFLFNSKGDLLIQQRADTKITFP 109



 Score = 35.1 bits (77), Expect = 0.052
 Identities = 11/18 (61%), Positives = 16/18 (88%)
 Frame = +2

Query: 410 YTNACCSHPLYIDEKPEE 463
           +TN+CCSHPLY++ + EE
Sbjct: 112 FTNSCCSHPLYVESEMEE 129


>SB_56787| Best HMM Match : Keratin_B2 (HMM E-Value=0.34)
          Length = 527

 Score = 30.3 bits (65), Expect = 1.5
 Identities = 21/91 (23%), Positives = 42/91 (46%)
 Frame = -2

Query: 314 HITIRSYFMTFSLCSRSYEVSFSSTSRQISLSKASAWANSRPSLSRVAGFTGSEAKNLFS 135
           H+TI S+ +  ++ SRS  V+ +S SR ++++  S               T +   N   
Sbjct: 337 HVTITSHHIKVTITSRSRHVTITSRSRHVTITSRSRHGTIISRSRHHDNITVTTCHN-HI 395

Query: 134 TLRVLHSDLVSFLTNIFVCMNFTELHFYITI 42
           T+   H+ + S   + ++ +  T  H +IT+
Sbjct: 396 TVTTCHNHITSHQGHNYITV--TTCHNHITV 424


>SB_35375| Best HMM Match : RVT_1 (HMM E-Value=0.00074)
          Length = 996

 Score = 29.5 bits (63), Expect = 2.6
 Identities = 12/31 (38%), Positives = 18/31 (58%)
 Frame = +3

Query: 63  FSKIHTYENVGQKANKITVEHPKSREKIFGF 155
           F +IH     G +  KIT  HPK++ ++ GF
Sbjct: 760 FHRIHRVHREGFRHKKITPRHPKAQGQVEGF 790


>SB_47598| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 2332

 Score = 27.9 bits (59), Expect = 7.9
 Identities = 17/38 (44%), Positives = 19/38 (50%)
 Frame = -2

Query: 620 GRTVCGXSXVRPTLPSPGSX*CTRVMTVTVPGPTDLVD 507
           GR VC  S     L SPGS  C  V T+ VPG   + D
Sbjct: 321 GRMVCICSDATVRLISPGSGAC--VTTLLVPGMQTIAD 356


  Database: nematostella
    Posted date:  Oct 22, 2007  1:22 PM
  Number of letters in database: 16,821,457
  Number of sequences in database:  59,808
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 20,160,494
Number of Sequences: 59808
Number of extensions: 422940
Number of successful extensions: 781
Number of sequences better than 10.0: 4
Number of HSP's better than 10.0 without gapping: 717
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 780
length of database: 16,821,457
effective HSP length: 79
effective length of database: 12,096,625
effective search space used: 1717720750
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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