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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= NV060421.seq
         (666 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At5g16440.1 68418.m01921 isopentenyl-diphosphate delta-isomerase...    53   1e-07
At3g02780.1 68416.m00270 isopentenyl-diphosphate delta-isomerase...    51   8e-07
At4g28190.1 68417.m04041 expressed protein                             28   6.4  
At3g06290.1 68416.m00722 SAC3/GANP family protein contains Pfam ...    28   6.4  
At5g12970.1 68418.m01487 C2 domain-containing protein contains I...    27   8.5  

>At5g16440.1 68418.m01921 isopentenyl-diphosphate delta-isomerase I
           / isopentenyl diphosphate:dimethylallyl diphosphate
           isomerase I (IPP1) identical to SP|Q38929
          Length = 233

 Score = 53.2 bits (122), Expect = 1e-07
 Identities = 25/49 (51%), Positives = 34/49 (69%), Gaps = 1/49 (2%)
 Frame = +3

Query: 261 IGTATKRECHKVGP-DGDVLLHRAFSVFLFNKRGDMFLQRRSSQKVTYP 404
           +G  TK  CH +   + + LLHRAFSVFLFN + ++ LQ+RS  KVT+P
Sbjct: 32  VGHDTKYNCHLMEKIEAENLLHRAFSVFLFNSKYELLLQQRSKTKVTFP 80



 Score = 30.7 bits (66), Expect = 0.91
 Identities = 15/34 (44%), Positives = 18/34 (52%), Gaps = 1/34 (2%)
 Frame = +2

Query: 383 KPKSNISRFYTNACCSHPLYID-EKPEEIITAAR 481
           K K      +TN CCSHPLY + E  EE +   R
Sbjct: 74  KTKVTFPLVWTNTCCSHPLYRESELIEENVLGVR 107


>At3g02780.1 68416.m00270 isopentenyl-diphosphate delta-isomerase II
           / isopentenyl diphosphate:dimethylallyl diphosphate
           isomerase II (IPP2) identical to isopentenyl
           diphosphate:dimethylallyl diphosphate isomerase (IPP2)
           GB:U49259 [Arabidopsis thaliana]
          Length = 284

 Score = 50.8 bits (116), Expect = 8e-07
 Identities = 24/49 (48%), Positives = 34/49 (69%), Gaps = 1/49 (2%)
 Frame = +3

Query: 261 IGTATKRECHKV-GPDGDVLLHRAFSVFLFNKRGDMFLQRRSSQKVTYP 404
           +G  +K  CH +   +   LLHRAFSVFLFN + ++ LQ+RS+ KVT+P
Sbjct: 83  VGHDSKYNCHLMENIEAKNLLHRAFSVFLFNSKYELLLQQRSNTKVTFP 131



 Score = 28.7 bits (61), Expect = 3.7
 Identities = 9/11 (81%), Positives = 10/11 (90%)
 Frame = +2

Query: 410 YTNACCSHPLY 442
           +TN CCSHPLY
Sbjct: 134 WTNTCCSHPLY 144


>At4g28190.1 68417.m04041 expressed protein
          Length = 237

 Score = 27.9 bits (59), Expect = 6.4
 Identities = 8/22 (36%), Positives = 15/22 (68%)
 Frame = -1

Query: 621 WKNSMWSIXGSPXTPITRVVIV 556
           WKN++W I G    P+++ V++
Sbjct: 91  WKNNVWVIIGGEKVPLSKTVLL 112


>At3g06290.1 68416.m00722 SAC3/GANP family protein contains Pfam
            profile: PF03399 SAC3/GANP family
          Length = 1720

 Score = 27.9 bits (59), Expect = 6.4
 Identities = 11/29 (37%), Positives = 21/29 (72%)
 Frame = -2

Query: 278  LCSRSYEVSFSSTSRQISLSKASAWANSR 192
            LC+++  V+ SS++ Q++ S AS W +S+
Sbjct: 1175 LCTQTKSVNSSSSASQVTHSAASRWLSSK 1203


>At5g12970.1 68418.m01487 C2 domain-containing protein contains
           INTERPRO:IPR000008 C2 domain
          Length = 769

 Score = 27.5 bits (58), Expect = 8.5
 Identities = 13/46 (28%), Positives = 22/46 (47%)
 Frame = +3

Query: 9   RRSRDTLLFILYCNVKV*FSKIHTYENVGQKANKITVEHPKSREKI 146
           R  R T LF+L+C +      +  ++ V   A    + HP+ R K+
Sbjct: 705 RDPRATTLFVLFCLIAAIVLYVTPFQVVALLAGIYVLRHPRFRHKL 750


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 14,044,276
Number of Sequences: 28952
Number of extensions: 285935
Number of successful extensions: 654
Number of sequences better than 10.0: 5
Number of HSP's better than 10.0 without gapping: 632
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 654
length of database: 12,070,560
effective HSP length: 78
effective length of database: 9,812,304
effective search space used: 1403159472
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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