Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= NV060416.seq
(554 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At4g39520.1 68417.m05588 GTP-binding protein, putative similar t... 111 3e-25
At1g17470.1 68414.m02143 developmentally regulated GTP-binding p... 105 3e-23
At1g72660.1 68414.m08403 developmentally regulated GTP-binding p... 103 6e-23
At5g43060.1 68418.m05256 cysteine proteinase, putative / thiol p... 28 3.6
At4g31490.1 68417.m04473 coatomer beta subunit, putative / beta-... 27 6.4
At4g31480.1 68417.m04472 coatomer beta subunit, putative / beta-... 27 6.4
At2g01440.1 68415.m00067 ATP-dependent DNA helicase, putative 27 6.4
At1g48540.2 68414.m05428 leucine-rich repeat family protein 27 6.4
At1g48540.1 68414.m05427 leucine-rich repeat family protein 27 6.4
At5g18570.1 68418.m02195 GTP1/OBG family protein similar to SP|P... 27 8.4
>At4g39520.1 68417.m05588 GTP-binding protein, putative similar to
SP|Q9Y295 Developmentally regulated GTP-binding protein
1 (DRG 1) {Homo sapiens}; contains Pfam profiles
PF02824: TGS domain, PF01018: GTP1/OBG family
Length = 369
Score = 111 bits (267), Expect = 3e-25
Identities = 58/88 (65%), Positives = 60/88 (68%), Gaps = 2/88 (2%)
Frame = +1
Query: 250 LGLLKAKLAKYRSQLLEPSKKGGD--KGEGFDVLKSGDARVALIGFPSVGKXXXXXXXXX 423
LGLLKAKLAK R LL P KGG GEGFDV KSGD+RV L+GFPSVGK
Sbjct: 28 LGLLKAKLAKLRRDLLAPPTKGGGGGAGEGFDVTKSGDSRVGLVGFPSVGKSTLLNKLTG 87
Query: 424 XXXEAASYEFTTLTCIPGVIEYRGXNIQ 507
E ASYEFTTLTCIPGVI YRG IQ
Sbjct: 88 TFSEVASYEFTTLTCIPGVITYRGAKIQ 115
Score = 31.1 bits (67), Expect = 0.52
Identities = 15/26 (57%), Positives = 20/26 (76%)
Frame = +3
Query: 477 SYRISRXQHTMLDLPGIIEGAAQGQG 554
+YR ++ Q +LDLPGIIEGA G+G
Sbjct: 108 TYRGAKIQ--LLDLPGIIEGAKDGKG 131
>At1g17470.1 68414.m02143 developmentally regulated GTP-binding
protein (DRG1) identical to developmentally regulated
GTP binding protein (DRG1) [Arabidopsis thaliana]
GI:2345150
Length = 399
Score = 105 bits (251), Expect = 3e-23
Identities = 54/86 (62%), Positives = 57/86 (66%)
Frame = +1
Query: 250 LGLLKAKLAKYRSQLLEPSKKGGDKGEGFDVLKSGDARVALIGFPSVGKXXXXXXXXXXX 429
LG LKAK+AK R+QLLEP K GEGF+V K G RVALIGFPSVGK
Sbjct: 27 LGQLKAKIAKLRTQLLEPPKGASGGGEGFEVTKYGHGRVALIGFPSVGKSTLLTMLTGTH 86
Query: 430 XEAASYEFTTLTCIPGVIEYRGXNIQ 507
EAASYEFTTLTCIPGVI Y IQ
Sbjct: 87 SEAASYEFTTLTCIPGVIHYNDTKIQ 112
Score = 32.3 bits (70), Expect = 0.22
Identities = 15/27 (55%), Positives = 16/27 (59%)
Frame = +2
Query: 173 MGXLXXXXXXXXXXARTQKNKATEYHL 253
MG + ARTQKNKATEYHL
Sbjct: 1 MGIIERIKEIEAEMARTQKNKATEYHL 27
Score = 31.5 bits (68), Expect = 0.39
Identities = 12/16 (75%), Positives = 16/16 (100%)
Frame = +3
Query: 507 MLDLPGIIEGAAQGQG 554
+LDLPGIIEGA++G+G
Sbjct: 113 LLDLPGIIEGASEGKG 128
>At1g72660.1 68414.m08403 developmentally regulated GTP-binding
protein, putative very strong similarity to
developmentally regulated GTP binding protein (DRG1)
[Arabidopsis thaliana] GI:2345150
Length = 399
Score = 103 bits (248), Expect = 6e-23
Identities = 53/86 (61%), Positives = 57/86 (66%)
Frame = +1
Query: 250 LGLLKAKLAKYRSQLLEPSKKGGDKGEGFDVLKSGDARVALIGFPSVGKXXXXXXXXXXX 429
LG LKAK+AK R+QLLEP K G+GF+V K G RVALIGFPSVGK
Sbjct: 27 LGQLKAKIAKLRTQLLEPPKGSSGGGDGFEVTKYGHGRVALIGFPSVGKSTLLTMLTGTH 86
Query: 430 XEAASYEFTTLTCIPGVIEYRGXNIQ 507
EAASYEFTTLTCIPGVI Y IQ
Sbjct: 87 SEAASYEFTTLTCIPGVIHYNDTKIQ 112
Score = 31.9 bits (69), Expect = 0.30
Identities = 15/27 (55%), Positives = 16/27 (59%)
Frame = +2
Query: 173 MGXLXXXXXXXXXXARTQKNKATEYHL 253
MG + ARTQKNKATEYHL
Sbjct: 1 MGIVERIKEIEAEMARTQKNKATEYHL 27
Score = 31.5 bits (68), Expect = 0.39
Identities = 12/16 (75%), Positives = 16/16 (100%)
Frame = +3
Query: 507 MLDLPGIIEGAAQGQG 554
+LDLPGIIEGA++G+G
Sbjct: 113 LLDLPGIIEGASEGKG 128
>At5g43060.1 68418.m05256 cysteine proteinase, putative / thiol
protease, putative similar to cysteine proteinase RD21A
precursor (thiol protease) GI:435619, SP:P43297 from
[Arabidopsis thaliana]
Length = 463
Score = 28.3 bits (60), Expect = 3.6
Identities = 14/35 (40%), Positives = 19/35 (54%)
Frame = -1
Query: 377 PIKATRASPDFSTSKPSPLSPPFFEGSSNCDLYFA 273
PIK + P+ S PSP+ PP + CD YF+
Sbjct: 352 PIKKGQNPPNPGPSPPSPIKPP-----TTCDKYFS 381
>At4g31490.1 68417.m04473 coatomer beta subunit, putative /
beta-coat protein, putative / beta-COP, putative similar
to Coatomer beta subunit (Beta-coat protein) (Beta-COP)
from {Rattus norvegicus} SP|P23514, {Mus musculus}
SP|Q9JIF7, {Homo sapiens} SP|P53618; contains Pfam
profile: PF01602 Adaptin N terminal region
Length = 948
Score = 27.5 bits (58), Expect = 6.4
Identities = 14/42 (33%), Positives = 24/42 (57%)
Frame = -2
Query: 388 QKESLSKQLEHHQTLVHRNLHLCLHLFLKVPATVIYILLIWL 263
Q L K E+ Q L+ +H C F +V +TV+++L+ +L
Sbjct: 364 QSGELEKNGEYRQMLIQA-IHACAVKFPEVASTVVHLLMDFL 404
>At4g31480.1 68417.m04472 coatomer beta subunit, putative /
beta-coat protein, putative / beta-COP, putative similar
to Coatomer beta subunit (Beta-coat protein) (Beta-COP)
from {Rattus norvegicus} SP|P23514, {Mus musculus}
SP|Q9JIF7, {Homo sapiens} SP|P53618; contains Pfam
profile: PF01602 Adaptin N terminal region
Length = 971
Score = 27.5 bits (58), Expect = 6.4
Identities = 14/42 (33%), Positives = 24/42 (57%)
Frame = -2
Query: 388 QKESLSKQLEHHQTLVHRNLHLCLHLFLKVPATVIYILLIWL 263
Q L K E+ Q L+ +H C F +V +TV+++L+ +L
Sbjct: 387 QSGELEKNGEYRQMLIQA-IHACAVKFPEVASTVVHLLMDFL 427
>At2g01440.1 68415.m00067 ATP-dependent DNA helicase, putative
Length = 953
Score = 27.5 bits (58), Expect = 6.4
Identities = 13/41 (31%), Positives = 20/41 (48%)
Frame = +1
Query: 250 LGLLKAKLAKYRSQLLEPSKKGGDKGEGFDVLKSGDARVAL 372
L ++ K KY LL K DK E + +SG+ ++ L
Sbjct: 787 LEIVTKKFPKYNCGLLHGRMKSDDKEEALNKFRSGETQILL 827
>At1g48540.2 68414.m05428 leucine-rich repeat family protein
Length = 1051
Score = 27.5 bits (58), Expect = 6.4
Identities = 10/20 (50%), Positives = 13/20 (65%)
Frame = -2
Query: 268 WLLRXQVIFSRFIFLCSRDF 209
WLLR + R +F+CS DF
Sbjct: 955 WLLRSLFVAGRRLFICSEDF 974
>At1g48540.1 68414.m05427 leucine-rich repeat family protein
Length = 1063
Score = 27.5 bits (58), Expect = 6.4
Identities = 10/20 (50%), Positives = 13/20 (65%)
Frame = -2
Query: 268 WLLRXQVIFSRFIFLCSRDF 209
WLLR + R +F+CS DF
Sbjct: 955 WLLRSLFVAGRRLFICSEDF 974
>At5g18570.1 68418.m02195 GTP1/OBG family protein similar to
SP|P20964 Spo0B-associated GTP-binding protein {Bacillus
subtilis}; contains Pfam profile PF01018: GTP1/OBG
family
Length = 681
Score = 27.1 bits (57), Expect = 8.4
Identities = 14/44 (31%), Positives = 20/44 (45%)
Frame = +1
Query: 358 ARVALIGFPSVGKXXXXXXXXXXXXEAASYEFTTLTCIPGVIEY 489
A V ++G P+ GK A+Y FTTL GV+ +
Sbjct: 379 ADVGIVGAPNAGKSTLLSVISAAQPTIANYPFTTLLPNLGVVSF 422
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 9,338,887
Number of Sequences: 28952
Number of extensions: 157422
Number of successful extensions: 452
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 421
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 450
length of database: 12,070,560
effective HSP length: 77
effective length of database: 9,841,256
effective search space used: 1053014392
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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