BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= NV060415.seq (567 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBC32H8.12c |act1|cps8|actin |Schizosaccharomyces pombe|chr 2||... 161 5e-41 SPAC11H11.06 |arp2|SPAC22F8.01|ARP2/3 actin-organizing complex s... 95 8e-21 SPAC630.03 |arp3|act2|actin-like protein Arp3|Schizosaccharomyce... 85 7e-18 SPBC1347.12 |||actin-like protein Arp1 |Schizosaccharomyces pomb... 83 2e-17 SPBP23A10.08 |alp5|arp4|actin-like protein Arp4|Schizosaccharomy... 55 8e-09 SPBC365.10 |||actin-like protein Arp5 |Schizosaccharomyces pombe... 48 1e-06 SPAC23D3.09 |arp42|arp4|SWI/SNF and RSC complex subunit Arp42|Sc... 45 7e-06 SPAPB18E9.04c |||sequence orphan|Schizosaccharomyces pombe|chr 1... 30 0.27 SPAC26A3.05 |chc1||clathrin heavy chain Chc1 |Schizosaccharomyce... 29 0.36 SPAC1071.06 |arp9||SWI/SNF and RSC complex subunit Arp9|Schizosa... 27 2.5 SPBC13E7.09 |vrp1||verprolin|Schizosaccharomyces pombe|chr 2|||M... 26 3.4 SPCC5E4.04 |cut1||separase|Schizosaccharomyces pombe|chr 3|||Manual 26 4.4 SPAC20G8.05c |cdc15||cell division control protein Cdc15|Schizos... 26 4.4 SPBC115.01c |rrp46||exosome subunit Rrp46 |Schizosaccharomyces p... 25 7.7 >SPBC32H8.12c |act1|cps8|actin |Schizosaccharomyces pombe|chr 2|||Manual Length = 375 Score = 161 bits (392), Expect = 5e-41 Identities = 73/84 (86%), Positives = 75/84 (89%) Frame = +3 Query: 255 DEAQSKRGILTLKYPIEHGIVTNWXDMXKIWHHTFYNELRVAPEXHPVLLTEAPLNPKAN 434 DEAQSKRGILTLKYPIEHGIV NW DM KIWHHTFYNELRVAPE HP LLTEAPLNPK+N Sbjct: 56 DEAQSKRGILTLKYPIEHGIVNNWDDMEKIWHHTFYNELRVAPEEHPCLLTEAPLNPKSN 115 Query: 435 XEKMTXIMFETFNTPAMYVAIQAV 506 EKMT I+FETFN PA YVAIQAV Sbjct: 116 REKMTQIIFETFNAPAFYVAIQAV 139 Score = 113 bits (273), Expect = 1e-26 Identities = 50/60 (83%), Positives = 56/60 (93%) Frame = +1 Query: 94 DEEVAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGMRHRAK 273 +EE+AALV+DNGSGMCKAGFAGDDAPRAVFPSIVGRPRH G+MVGMGQKDSYVG ++K Sbjct: 2 EEEIAALVIDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHHGIMVGMGQKDSYVGDEAQSK 61 Score = 39.5 bits (88), Expect = 3e-04 Identities = 18/19 (94%), Positives = 18/19 (94%) Frame = +2 Query: 509 SLYASGRTXGIVLDSGDGV 565 SLYASGRT GIVLDSGDGV Sbjct: 141 SLYASGRTTGIVLDSGDGV 159 >SPAC11H11.06 |arp2|SPAC22F8.01|ARP2/3 actin-organizing complex subunit Arp2|Schizosaccharomyces pombe|chr 1|||Manual Length = 390 Score = 94.7 bits (225), Expect = 8e-21 Identities = 40/97 (41%), Positives = 65/97 (67%) Frame = +3 Query: 216 RDGRYGTEGLLCRDEAQSKRGILTLKYPIEHGIVTNWXDMXKIWHHTFYNELRVAPEXHP 395 + G + ++ DEA++ R +L +KYP+E+GI+ ++ +M ++W +TF+ +L++ P Sbjct: 43 KTGNVQIKDVMVGDEAEAVRSLLQVKYPMENGIIRDFEEMNQLWDYTFFEKLKIDPRGRK 102 Query: 396 VLLTEAPLNPKANXEKMTXIMFETFNTPAMYVAIQAV 506 +LLTE P+NP AN EKM MFE + +YVAIQAV Sbjct: 103 ILLTEPPMNPVANREKMCETMFERYGFGGVYVAIQAV 139 Score = 48.0 bits (109), Expect = 1e-06 Identities = 21/35 (60%), Positives = 26/35 (74%) Frame = +1 Query: 100 EVAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRP 204 E A +V+DNG+G K G+A D+ PR FPSIVGRP Sbjct: 2 ESAPIVLDNGTGFVKVGYAKDNFPRFQFPSIVGRP 36 Score = 31.9 bits (69), Expect = 0.067 Identities = 13/19 (68%), Positives = 16/19 (84%) Frame = +2 Query: 509 SLYASGRTXGIVLDSGDGV 565 SLYA G + G+V+DSGDGV Sbjct: 141 SLYAQGLSSGVVVDSGDGV 159 >SPAC630.03 |arp3|act2|actin-like protein Arp3|Schizosaccharomyces pombe|chr 1|||Manual Length = 427 Score = 85.0 bits (201), Expect = 7e-18 Identities = 36/74 (48%), Positives = 45/74 (60%) Frame = +3 Query: 285 TLKYPIEHGIVTNWXDMXKIWHHTFYNELRVAPEXHPVLLTEAPLNPKANXEKMTXIMFE 464 +L YPI HG + NW M + W + + LR PE H LLTE PLNP N E IMFE Sbjct: 89 SLDYPIRHGQIENWDHMERFWQQSLFKYLRCEPEDHYFLLTEPPLNPPENRENTAEIMFE 148 Query: 465 TFNTPAMYVAIQAV 506 +FN +Y+A+QAV Sbjct: 149 SFNCAGLYIAVQAV 162 Score = 42.7 bits (96), Expect = 4e-05 Identities = 20/52 (38%), Positives = 32/52 (61%), Gaps = 2/52 (3%) Frame = +1 Query: 112 LVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVG--MGQKDSYVGMR 261 +++DNG+G K G+AG+DAP VFP+++ R G G + K SY+ + Sbjct: 8 IIMDNGTGYSKLGYAGNDAPSYVFPTVIA-TRSAGASSGPAVSSKPSYMASK 58 >SPBC1347.12 |||actin-like protein Arp1 |Schizosaccharomyces pombe|chr 2|||Manual Length = 379 Score = 83.4 bits (197), Expect = 2e-17 Identities = 38/84 (45%), Positives = 54/84 (64%), Gaps = 1/84 (1%) Frame = +3 Query: 258 EAQSKRGILTLKYPIEHGIVTNWXDMXKIWHHTFYNE-LRVAPEXHPVLLTEAPLNPKAN 434 EAQ+ RG+L ++ PIE GI+ NW DM +IW + + ++ L PE HP+LLTE PL N Sbjct: 61 EAQNLRGLLKIQRPIERGIIQNWSDMEEIWSYIYSDQQLNTLPEEHPLLLTEPPLANIRN 120 Query: 435 XEKMTXIMFETFNTPAMYVAIQAV 506 EK+ +ET N PA+ ++Q V Sbjct: 121 KEKIAEYFYETLNVPALSFSLQPV 144 Score = 63.7 bits (148), Expect = 2e-11 Identities = 27/48 (56%), Positives = 35/48 (72%) Frame = +1 Query: 112 LVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVG 255 + +DNGSG KAGFAGDD P+ +FP+ VGR +H+ VM QKD +VG Sbjct: 12 ICIDNGSGFIKAGFAGDDIPKCLFPTCVGRIKHERVMPSSIQKDMFVG 59 Score = 31.1 bits (67), Expect = 0.12 Identities = 13/19 (68%), Positives = 16/19 (84%) Frame = +2 Query: 509 SLYASGRTXGIVLDSGDGV 565 +LYAS RT GIVL+ GDG+ Sbjct: 146 ALYASARTTGIVLECGDGL 164 >SPBP23A10.08 |alp5|arp4|actin-like protein Arp4|Schizosaccharomyces pombe|chr 2|||Manual Length = 433 Score = 54.8 bits (126), Expect = 8e-09 Identities = 26/73 (35%), Positives = 37/73 (50%) Frame = +3 Query: 282 LTLKYPIEHGIVTNWXDMXKIWHHTFYNELRVAPEXHPVLLTEAPLNPKANXEKMTXIMF 461 + +K I +G V NW +W + +L+ P HP+L+TE NP N K MF Sbjct: 63 MEIKNAIRNGWVENWDVTVDLWRYGLEQQLKTNPLEHPILITEPFDNPPENRVKTLETMF 122 Query: 462 ETFNTPAMYVAIQ 500 E+ PA Y+A Q Sbjct: 123 ESLRCPATYLAKQ 135 Score = 40.3 bits (90), Expect = 2e-04 Identities = 16/34 (47%), Positives = 24/34 (70%) Frame = +1 Query: 97 EEVAALVVDNGSGMCKAGFAGDDAPRAVFPSIVG 198 +EV+A+V+D GS + GF+G+D P+ V PS G Sbjct: 9 DEVSAIVIDPGSKWTRIGFSGEDIPKCVLPSYCG 42 >SPBC365.10 |||actin-like protein Arp5 |Schizosaccharomyces pombe|chr 2|||Manual Length = 721 Score = 47.6 bits (108), Expect = 1e-06 Identities = 21/70 (30%), Positives = 37/70 (52%) Frame = +3 Query: 297 PIEHGIVTNWXDMXKIWHHTFYNELRVAPEXHPVLLTEAPLNPKANXEKMTXIMFETFNT 476 P E +++NW M ++ +TF +L + HP+ +TE NP MT ++FE +N Sbjct: 86 PFERNVISNWDLMEQVLDYTFL-KLGIDRMEHPICMTEPLANPTYVRSTMTELLFELYNA 144 Query: 477 PAMYVAIQAV 506 P++ I + Sbjct: 145 PSVAYGIDGL 154 Score = 35.9 bits (79), Expect = 0.004 Identities = 15/32 (46%), Positives = 22/32 (68%) Frame = +1 Query: 112 LVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPR 207 LV+DNGS +AG+ G+ P+ VF ++V R R Sbjct: 27 LVIDNGSWQLRAGWGGEKDPKLVFDNLVSRYR 58 >SPAC23D3.09 |arp42|arp4|SWI/SNF and RSC complex subunit Arp42|Schizosaccharomyces pombe|chr 1|||Manual Length = 430 Score = 45.2 bits (102), Expect = 7e-06 Identities = 19/67 (28%), Positives = 35/67 (52%) Frame = +3 Query: 306 HGIVTNWXDMXKIWHHTFYNELRVAPEXHPVLLTEAPLNPKANXEKMTXIMFETFNTPAM 485 +GI+ +W W +L+V P + +++TE NP++ +++ FE + PA Sbjct: 74 NGIIQDWESTLYTWERGLKEKLQVNPTEYAMMITEPSWNPQSVRQQIMEAAFEQLHVPAF 133 Query: 486 YVAIQAV 506 Y+ QAV Sbjct: 134 YLTKQAV 140 Score = 36.7 bits (81), Expect = 0.002 Identities = 14/34 (41%), Positives = 23/34 (67%) Frame = +1 Query: 97 EEVAALVVDNGSGMCKAGFAGDDAPRAVFPSIVG 198 EE+ +LV+D GS + G+AG+++P + PS G Sbjct: 8 EEIPSLVIDPGSCWTRFGYAGEESPMTILPSYYG 41 >SPAPB18E9.04c |||sequence orphan|Schizosaccharomyces pombe|chr 1|||Manual Length = 800 Score = 29.9 bits (64), Expect = 0.27 Identities = 19/55 (34%), Positives = 23/55 (41%), Gaps = 1/55 (1%) Frame = -2 Query: 254 PT*ESFCPI-PTITP*WRGLPTIEGNTARGASSPAKPALHIPEPLSTTNAATSSS 93 PT S P+ PT+ P P N S P PL+TTN TS+S Sbjct: 401 PTGNSTTPVTPTVPPTSSSTPLTTTNCTTSTSVPYTSTPVTSTPLATTNCTTSTS 455 >SPAC26A3.05 |chc1||clathrin heavy chain Chc1 |Schizosaccharomyces pombe|chr 1|||Manual Length = 1666 Score = 29.5 bits (63), Expect = 0.36 Identities = 19/53 (35%), Positives = 29/53 (54%), Gaps = 5/53 (9%) Frame = +3 Query: 357 FYNELRVAPEXHPVLLTE--APLNPKANXEKMTXIMFETFNTPAM---YVAIQ 500 +Y L E HP+LLT+ A L P+ + ++ I ++ NTP + VAIQ Sbjct: 1399 YYRALNFYLEQHPMLLTDLLAALTPRIDHPRVIRIFEKSENTPLILNFMVAIQ 1451 >SPAC1071.06 |arp9||SWI/SNF and RSC complex subunit Arp9|Schizosaccharomyces pombe|chr 1|||Manual Length = 523 Score = 26.6 bits (56), Expect = 2.5 Identities = 19/72 (26%), Positives = 27/72 (37%), Gaps = 4/72 (5%) Frame = +3 Query: 297 PIEHGIVTNWXDMXKIWHHTFYNELRVAPE----XHPVLLTEAPLNPKANXEKMTXIMFE 464 PI+ G V +W + W H Y+ L P +PV L + E T FE Sbjct: 113 PIQRGRVVDWEALKAFWKH-LYSLLLKDPNDTTFRYPVCLVIPTYWSLYDRELATQFFFE 171 Query: 465 TFNTPAMYVAIQ 500 P +A + Sbjct: 172 ECQVPGFTIAYE 183 >SPBC13E7.09 |vrp1||verprolin|Schizosaccharomyces pombe|chr 2|||Manual Length = 309 Score = 26.2 bits (55), Expect = 3.4 Identities = 13/31 (41%), Positives = 16/31 (51%) Frame = -2 Query: 233 PIPTITP*WRGLPTIEGNTARGASSPAKPAL 141 P+PT P LPT NT + P+ PAL Sbjct: 74 PVPTGAP---SLPTSSNNTQQAEERPSMPAL 101 >SPCC5E4.04 |cut1||separase|Schizosaccharomyces pombe|chr 3|||Manual Length = 1828 Score = 25.8 bits (54), Expect = 4.4 Identities = 12/23 (52%), Positives = 14/23 (60%) Frame = -3 Query: 139 TYRSHCLLPTRQLLRRTS*ISEI 71 TYR H +PT LL T +SEI Sbjct: 168 TYRDHDAIPTATLLNPTLDVSEI 190 >SPAC20G8.05c |cdc15||cell division control protein Cdc15|Schizosaccharomyces pombe|chr 1|||Manual Length = 927 Score = 25.8 bits (54), Expect = 4.4 Identities = 12/26 (46%), Positives = 14/26 (53%) Frame = -3 Query: 253 LHKSPSVPYRPSRPDGGAFPRSRGTR 176 + K+PS PY SRP A S TR Sbjct: 310 ISKAPSYPYSSSRPSASASLASSPTR 335 >SPBC115.01c |rrp46||exosome subunit Rrp46 |Schizosaccharomyces pombe|chr 2|||Manual Length = 226 Score = 25.0 bits (52), Expect = 7.7 Identities = 10/25 (40%), Positives = 17/25 (68%) Frame = +3 Query: 399 LLTEAPLNPKANXEKMTXIMFETFN 473 +L APL+ + +KM ++FET+N Sbjct: 181 VLETAPLHAEEVSKKMKELLFETYN 205 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,294,472 Number of Sequences: 5004 Number of extensions: 46133 Number of successful extensions: 120 Number of sequences better than 10.0: 14 Number of HSP's better than 10.0 without gapping: 99 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 117 length of database: 2,362,478 effective HSP length: 69 effective length of database: 2,017,202 effective search space used: 240047038 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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