BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= NV060407.seq (570 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPCP31B10.07 |eft202||translation elongation factor 2 |Schizosac... 105 6e-24 SPAC513.01c |eft201|eft2-1, etf2, SPAPYUK71.04c|translation elon... 105 6e-24 SPCC553.08c |||GTPase Ria1 |Schizosaccharomyces pombe|chr 3|||Ma... 66 3e-12 SPBC1306.01c ||SPBC409.22c|translation elongation factor G|Schiz... 50 2e-07 SPAC1B3.04c |||mitochondrial GTPase Guf1 |Schizosaccharomyces po... 46 5e-06 SPBC660.10 |||translation elongation factor G|Schizosaccharomyce... 38 8e-04 SPBC215.12 |cwf10|spef2, snu114|GTPase Cwf10 |Schizosaccharomyce... 38 0.001 SPAC23A1.10 |ef1a-b||translation elongation factor EF-1 alpha Ef... 34 0.017 SPBC9B6.04c |tuf1||mitochondrial translation elongation factor E... 34 0.017 SPCC794.09c |ef1a-a||translation elongation factor EF-1 alpha Ef... 34 0.017 SPBC839.15c |ef1a-c||translation elongation factor EF-1 alpha Ef... 34 0.017 SPAC17A2.10c |||sequence orphan|Schizosaccharomyces pombe|chr 1|... 29 0.48 SPBC1271.15c |||translation initiation factor IF-2Mt|Schizosacch... 28 1.1 SPBC25H2.15 |||programmed cell death protein homolog|Schizosacch... 27 1.5 SPCC584.04 |sup35|erf3|translation release factor eRF3 |Schizosa... 26 3.4 SPBC30B4.01c |wsc1|SPBC3D6.14c|transmembrane receptor Wsc1 |Schi... 26 4.5 SPBC25B2.01 ||SPBC2G5.08|elongation factor 1 alpha related prote... 25 5.9 SPAC25G10.02 |cce1|ydc2|mitochondrial cruciform cutting endonucl... 25 5.9 SPBC19F5.03 |||inositol polyphosphate phosphatase |Schizosacchar... 25 5.9 SPCC1235.05c |fft2||fun thirty related protein Fft2|Schizosaccha... 25 5.9 SPAP14E8.02 |||transcription factor |Schizosaccharomyces pombe|c... 25 5.9 SPBC17G9.09 |tif213||translation initiation factor eIF2 gamma su... 25 7.8 >SPCP31B10.07 |eft202||translation elongation factor 2 |Schizosaccharomyces pombe|chr 3|||Manual Length = 842 Score = 105 bits (251), Expect = 6e-24 Identities = 48/70 (68%), Positives = 58/70 (82%) Frame = +1 Query: 49 MVNFTVDEIRGMMDKKRNIRNMSVIAHVDHGKSTLTDSLVSKAGIIAGARAGETRFTDTR 228 MV FT +E+R +M K N+RNMSVIAHVDHGKSTLTDSLV KAGII+ A+AG+ RF DTR Sbjct: 1 MVAFTPEEVRNLMGKPSNVRNMSVIAHVDHGKSTLTDSLVQKAGIISAAKAGDARFMDTR 60 Query: 229 KDEQDRCITL 258 DEQ+R +T+ Sbjct: 61 ADEQERGVTI 70 Score = 74.5 bits (175), Expect = 1e-14 Identities = 40/62 (64%), Positives = 48/62 (77%), Gaps = 1/62 (1%) Frame = +3 Query: 255 IKSTAISMFFELEEKDLVFITNPDQREKSE-KGFLINLIDSPGHVDFSSEVTAALRVTDG 431 IKSTAIS+F E+ + D+ D +E ++ FL+NLIDSPGHVDFSSEVTAALRVTDG Sbjct: 70 IKSTAISLFAEMTDDDM-----KDMKEPADGTDFLVNLIDSPGHVDFSSEVTAALRVTDG 124 Query: 432 AL 437 AL Sbjct: 125 AL 126 Score = 29.1 bits (62), Expect = 0.48 Identities = 16/35 (45%), Positives = 21/35 (60%) Frame = +2 Query: 464 VCTKPKTVLRQAIAEASSLFCS*TKWNGALLELQL 568 VC + +TVLRQA+ E K + ALLELQ+ Sbjct: 135 VCVQTETVLRQALGERIRPVVVVNKVDRALLELQI 169 >SPAC513.01c |eft201|eft2-1, etf2, SPAPYUK71.04c|translation elongation factor 2 |Schizosaccharomyces pombe|chr 1|||Manual Length = 842 Score = 105 bits (251), Expect = 6e-24 Identities = 48/70 (68%), Positives = 58/70 (82%) Frame = +1 Query: 49 MVNFTVDEIRGMMDKKRNIRNMSVIAHVDHGKSTLTDSLVSKAGIIAGARAGETRFTDTR 228 MV FT +E+R +M K N+RNMSVIAHVDHGKSTLTDSLV KAGII+ A+AG+ RF DTR Sbjct: 1 MVAFTPEEVRNLMGKPSNVRNMSVIAHVDHGKSTLTDSLVQKAGIISAAKAGDARFMDTR 60 Query: 229 KDEQDRCITL 258 DEQ+R +T+ Sbjct: 61 ADEQERGVTI 70 Score = 74.5 bits (175), Expect = 1e-14 Identities = 40/62 (64%), Positives = 48/62 (77%), Gaps = 1/62 (1%) Frame = +3 Query: 255 IKSTAISMFFELEEKDLVFITNPDQREKSE-KGFLINLIDSPGHVDFSSEVTAALRVTDG 431 IKSTAIS+F E+ + D+ D +E ++ FL+NLIDSPGHVDFSSEVTAALRVTDG Sbjct: 70 IKSTAISLFAEMTDDDM-----KDMKEPADGTDFLVNLIDSPGHVDFSSEVTAALRVTDG 124 Query: 432 AL 437 AL Sbjct: 125 AL 126 Score = 29.1 bits (62), Expect = 0.48 Identities = 16/35 (45%), Positives = 21/35 (60%) Frame = +2 Query: 464 VCTKPKTVLRQAIAEASSLFCS*TKWNGALLELQL 568 VC + +TVLRQA+ E K + ALLELQ+ Sbjct: 135 VCVQTETVLRQALGERIRPVVVVNKVDRALLELQI 169 >SPCC553.08c |||GTPase Ria1 |Schizosaccharomyces pombe|chr 3|||Manual Length = 1000 Score = 66.5 bits (155), Expect = 3e-12 Identities = 30/64 (46%), Positives = 44/64 (68%) Frame = +1 Query: 67 DEIRGMMDKKRNIRNMSVIAHVDHGKSTLTDSLVSKAGIIAGARAGETRFTDTRKDEQDR 246 +++ + + NIRN +++AHVDHGK+TL DSL++ GII+ AG RF D R+DE R Sbjct: 7 EKLVSLQKNQENIRNFTLLAHVDHGKTTLADSLLASNGIISSKLAGTVRFLDFREDEITR 66 Query: 247 CITL 258 IT+ Sbjct: 67 GITM 70 Score = 60.9 bits (141), Expect = 1e-10 Identities = 33/60 (55%), Positives = 46/60 (76%) Frame = +3 Query: 255 IKSTAISMFFELEEKDLVFITNPDQREKSEKGFLINLIDSPGHVDFSSEVTAALRVTDGA 434 +KS+AIS+FF++ I+ D++ + EK +LINLIDSPGHVDFSSEV++A R+ DGA Sbjct: 70 MKSSAISLFFKV-------ISQNDEK-RVEKDYLINLIDSPGHVDFSSEVSSASRLCDGA 121 >SPBC1306.01c ||SPBC409.22c|translation elongation factor G|Schizosaccharomyces pombe|chr 2|||Manual Length = 770 Score = 50.4 bits (115), Expect = 2e-07 Identities = 22/40 (55%), Positives = 29/40 (72%) Frame = +3 Query: 318 NPDQREKSEKGFLINLIDSPGHVDFSSEVTAALRVTDGAL 437 N Q+ EK + IN+ID+PGH+DF+ EV ALRV DGA+ Sbjct: 134 NEKQKTDFEKSYNINIIDTPGHIDFTIEVERALRVLDGAV 173 Score = 35.1 bits (77), Expect = 0.007 Identities = 18/46 (39%), Positives = 26/46 (56%), Gaps = 2/46 (4%) Frame = +1 Query: 55 NFTVDEIRGMMDKKR--NIRNMSVIAHVDHGKSTLTDSLVSKAGII 186 N + E DKKR IRN+ + AH+D GK+T T+ ++ G I Sbjct: 41 NLNIQEQLNDNDKKRLKQIRNIGISAHIDSGKTTFTERVLYYTGRI 86 >SPAC1B3.04c |||mitochondrial GTPase Guf1 |Schizosaccharomyces pombe|chr 1|||Manual Length = 646 Score = 45.6 bits (103), Expect = 5e-06 Identities = 24/62 (38%), Positives = 38/62 (61%) Frame = +1 Query: 73 IRGMMDKKRNIRNMSVIAHVDHGKSTLTDSLVSKAGIIAGARAGETRFTDTRKDEQDRCI 252 +RG+ + +RN +VIAH+DHGKSTL+D ++ G+I +F D + E+ R I Sbjct: 50 VRGIPQNR--VRNWAVIAHIDHGKSTLSDCILKLTGVI-NEHNFRNQFLDKLEVERRRGI 106 Query: 253 TL 258 T+ Sbjct: 107 TV 108 Score = 39.1 bits (87), Expect = 4e-04 Identities = 15/31 (48%), Positives = 23/31 (74%) Frame = +3 Query: 345 KGFLINLIDSPGHVDFSSEVTAALRVTDGAL 437 + +L+NLID+PGHVDF +EV +L +G + Sbjct: 122 QSYLLNLIDTPGHVDFRAEVMHSLAACEGCI 152 >SPBC660.10 |||translation elongation factor G|Schizosaccharomyces pombe|chr 2|||Manual Length = 813 Score = 38.3 bits (85), Expect = 8e-04 Identities = 16/27 (59%), Positives = 21/27 (77%) Frame = +3 Query: 357 INLIDSPGHVDFSSEVTAALRVTDGAL 437 INLID+PGH DF+ EV ++ V DGA+ Sbjct: 95 INLIDTPGHADFTFEVERSVAVLDGAV 121 Score = 37.5 bits (83), Expect = 0.001 Identities = 21/57 (36%), Positives = 32/57 (56%), Gaps = 3/57 (5%) Frame = +1 Query: 100 NIRNMSVIAHVDHGKSTLTDSLVSKAGI---IAGARAGETRFTDTRKDEQDRCITLN 261 +IRN+ +IAH+D GK+TLT+ ++ G G+T D E+ R IT+N Sbjct: 27 SIRNVGIIAHIDAGKTTLTEKMLYYGGFTSHFGNVDTGDT-VMDYLPAERQRGITIN 82 >SPBC215.12 |cwf10|spef2, snu114|GTPase Cwf10 |Schizosaccharomyces pombe|chr 2|||Manual Length = 983 Score = 37.5 bits (83), Expect = 0.001 Identities = 15/31 (48%), Positives = 20/31 (64%) Frame = +3 Query: 345 KGFLINLIDSPGHVDFSSEVTAALRVTDGAL 437 K F ID+PGHVDF EV A + ++DG + Sbjct: 207 KTFAFQCIDTPGHVDFVDEVAAPMAISDGVV 237 >SPAC23A1.10 |ef1a-b||translation elongation factor EF-1 alpha Ef1a-b |Schizosaccharomyces pombe|chr 1|||Manual Length = 460 Score = 33.9 bits (74), Expect = 0.017 Identities = 16/32 (50%), Positives = 20/32 (62%) Frame = +1 Query: 85 MDKKRNIRNMSVIAHVDHGKSTLTDSLVSKAG 180 M K++ N+ VI HVD GKST T L+ K G Sbjct: 1 MGKEKGHINVVVIGHVDSGKSTTTGHLIYKCG 32 >SPBC9B6.04c |tuf1||mitochondrial translation elongation factor EF-Tu Tuf1 |Schizosaccharomyces pombe|chr 2|||Manual Length = 439 Score = 33.9 bits (74), Expect = 0.017 Identities = 19/57 (33%), Positives = 31/57 (54%) Frame = +1 Query: 91 KKRNIRNMSVIAHVDHGKSTLTDSLVSKAGIIAGARAGETRFTDTRKDEQDRCITLN 261 KK ++ N+ I HVDHGK+TLT ++ + A + D +E+ R IT++ Sbjct: 50 KKPHV-NIGTIGHVDHGKTTLTAAITKCLSDLGQASFMDYSQIDKAPEEKARGITIS 105 >SPCC794.09c |ef1a-a||translation elongation factor EF-1 alpha Ef1a-a |Schizosaccharomyces pombe|chr 3|||Manual Length = 460 Score = 33.9 bits (74), Expect = 0.017 Identities = 16/32 (50%), Positives = 20/32 (62%) Frame = +1 Query: 85 MDKKRNIRNMSVIAHVDHGKSTLTDSLVSKAG 180 M K++ N+ VI HVD GKST T L+ K G Sbjct: 1 MGKEKGHINVVVIGHVDSGKSTTTGHLIYKCG 32 >SPBC839.15c |ef1a-c||translation elongation factor EF-1 alpha Ef1a-c |Schizosaccharomyces pombe|chr 2|||Manual Length = 460 Score = 33.9 bits (74), Expect = 0.017 Identities = 16/32 (50%), Positives = 20/32 (62%) Frame = +1 Query: 85 MDKKRNIRNMSVIAHVDHGKSTLTDSLVSKAG 180 M K++ N+ VI HVD GKST T L+ K G Sbjct: 1 MGKEKGHINVVVIGHVDSGKSTTTGHLIYKCG 32 >SPAC17A2.10c |||sequence orphan|Schizosaccharomyces pombe|chr 1|||Manual Length = 230 Score = 29.1 bits (62), Expect = 0.48 Identities = 12/24 (50%), Positives = 16/24 (66%) Frame = -3 Query: 511 CLGNSLTQYCFRFCTHTPDTQSTT 440 CL ++ + CF FCTH PDT T+ Sbjct: 54 CLFSNFSHSCFLFCTH-PDTLPTS 76 >SPBC1271.15c |||translation initiation factor IF-2Mt|Schizosaccharomyces pombe|chr 2|||Manual Length = 686 Score = 27.9 bits (59), Expect = 1.1 Identities = 13/34 (38%), Positives = 22/34 (64%) Frame = +1 Query: 112 MSVIAHVDHGKSTLTDSLVSKAGIIAGARAGETR 213 ++++ HVDHGK+TL D+ K+ I + G T+ Sbjct: 174 VTLMGHVDHGKTTLLDAF-RKSTIASTEHGGITQ 206 >SPBC25H2.15 |||programmed cell death protein homolog|Schizosaccharomyces pombe|chr 2|||Manual Length = 396 Score = 27.5 bits (58), Expect = 1.5 Identities = 16/82 (19%), Positives = 40/82 (48%) Frame = +1 Query: 103 IRNMSVIAHVDHGKSTLTDSLVSKAGIIAGARAGETRFTDTRKDEQDRCITLNLRPSLCS 282 +++ I+H++ KS+ + V+ I + + G F+D ++ ++ PS S Sbjct: 112 VKSPKAISHLEEKKSSPKEKKVNPFAITSESSRGLNPFSDATSANNPFSLSTDVNPSKPS 171 Query: 283 SSLKRKI*YSSQTLTSVKRVRK 348 S++ K ++++ S+ +K Sbjct: 172 SNVFSKPSFAAKAQQSITDQQK 193 >SPCC584.04 |sup35|erf3|translation release factor eRF3 |Schizosaccharomyces pombe|chr 3|||Manual Length = 662 Score = 26.2 bits (55), Expect = 3.4 Identities = 11/26 (42%), Positives = 17/26 (65%) Frame = +1 Query: 109 NMSVIAHVDHGKSTLTDSLVSKAGII 186 N+ I HVD GKSTL +++ G++ Sbjct: 240 NIVFIGHVDAGKSTLGGNILFLTGMV 265 >SPBC30B4.01c |wsc1|SPBC3D6.14c|transmembrane receptor Wsc1 |Schizosaccharomyces pombe|chr 2|||Manual Length = 374 Score = 25.8 bits (54), Expect = 4.5 Identities = 19/76 (25%), Positives = 32/76 (42%) Frame = -3 Query: 505 GNSLTQYCFRFCTHTPDTQSTTTRAPSVTRSAAVTSEEKSTCPGESIKLIKKPFSLFSRW 326 GN + Q + + T S+++ +PS + + TS S+ S S S Sbjct: 119 GNGVLQTTVSSSSVSSTTSSSSSSSPSSSSTTTTTSPSSSSSSSSSSSSSSSSSSSSSSS 178 Query: 325 SGFVMNTKSFSSSSKN 278 S ++ S SSSS + Sbjct: 179 SSSSSSSSSSSSSSSS 194 Score = 25.8 bits (54), Expect = 4.5 Identities = 17/64 (26%), Positives = 30/64 (46%) Frame = -3 Query: 454 TQSTTTRAPSVTRSAAVTSEEKSTCPGESIKLIKKPFSLFSRWSGFVMNTKSFSSSSKNI 275 + +TTT +PS + S++ +S S+ S S S S ++ S SSSS + Sbjct: 147 SSTTTTTSPSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSS 206 Query: 274 EMAV 263 + + Sbjct: 207 SVPI 210 >SPBC25B2.01 ||SPBC2G5.08|elongation factor 1 alpha related protein|Schizosaccharomyces pombe|chr 2|||Manual Length = 592 Score = 25.4 bits (53), Expect = 5.9 Identities = 27/104 (25%), Positives = 49/104 (47%), Gaps = 15/104 (14%) Frame = +1 Query: 28 KNHKPSKMVNFTVDEIRGMMDKKRNIRNMSVIAHVDHGKSTLTDSLVSKAGII------- 186 K P+ +V+ V EI + K + ++ V HVD GKST+ ++ + G I Sbjct: 155 KKQNPTDLVS--VPEIFEQSNPKPVV-HLVVTGHVDSGKSTMLGRIMFELGEINSRSMQK 211 Query: 187 ---AGARAGETRFT-----DTRKDEQDRCITLNLRPSLCSSSLK 294 A +G+ F+ DT ++E+ R +T+++ + S K Sbjct: 212 LHNEAANSGKGSFSYAWLLDTTEEERARGVTMDVASTTFESDKK 255 >SPAC25G10.02 |cce1|ydc2|mitochondrial cruciform cutting endonuclease Cce1|Schizosaccharomyces pombe|chr 1|||Manual Length = 258 Score = 25.4 bits (53), Expect = 5.9 Identities = 13/33 (39%), Positives = 20/33 (60%), Gaps = 1/33 (3%) Frame = -3 Query: 349 PFSLFSRWSGFVMNTK-SFSSSSKNIEMAVDLM 254 P S +S W+ V+NTK SFS ++M +L+ Sbjct: 168 PKSTYSYWAS-VLNTKASFSKKKSRVQMVKELI 199 >SPBC19F5.03 |||inositol polyphosphate phosphatase |Schizosaccharomyces pombe|chr 2|||Manual Length = 598 Score = 25.4 bits (53), Expect = 5.9 Identities = 11/32 (34%), Positives = 19/32 (59%) Frame = -1 Query: 105 DIPLLVHHPTDLVYREIHHFRWFMIFVLLNQL 10 D P + +H D ++E H RW + +LLN++ Sbjct: 318 DNPHIHYHYFDF-HKECSHMRWDRVSLLLNEI 348 >SPCC1235.05c |fft2||fun thirty related protein Fft2|Schizosaccharomyces pombe|chr 3|||Manual Length = 1284 Score = 25.4 bits (53), Expect = 5.9 Identities = 12/28 (42%), Positives = 16/28 (57%) Frame = -3 Query: 100 SASCPSSHGSRLP*NSPF*MVYDFCSIK 17 +ASCP SH L + PF + + C IK Sbjct: 417 TASCPLSHSKLLLEHRPFQTLAEACIIK 444 >SPAP14E8.02 |||transcription factor |Schizosaccharomyces pombe|chr 1|||Manual Length = 566 Score = 25.4 bits (53), Expect = 5.9 Identities = 13/37 (35%), Positives = 21/37 (56%), Gaps = 1/37 (2%) Frame = +2 Query: 134 ITASQPSRTRWFPR-PVSLLVREPERPVSLTRVRTNK 241 +T S T + P P S + REP P+S R+R+++ Sbjct: 48 LTPEPSSNTFYAPSSPASAVRREPLSPMSFVRMRSHR 84 >SPBC17G9.09 |tif213||translation initiation factor eIF2 gamma subunit|Schizosaccharomyces pombe|chr 2|||Manual Length = 446 Score = 25.0 bits (52), Expect = 7.8 Identities = 9/19 (47%), Positives = 13/19 (68%) Frame = +1 Query: 109 NMSVIAHVDHGKSTLTDSL 165 N+ I HV HGKST+ ++ Sbjct: 25 NIGTIGHVAHGKSTVVKAI 43 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,316,970 Number of Sequences: 5004 Number of extensions: 44944 Number of successful extensions: 160 Number of sequences better than 10.0: 22 Number of HSP's better than 10.0 without gapping: 141 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 158 length of database: 2,362,478 effective HSP length: 69 effective length of database: 2,017,202 effective search space used: 242064240 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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