BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= NV060405.seq (573 letters) Database: nematostella 59,808 sequences; 16,821,457 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SB_45540| Best HMM Match : HSP70 (HMM E-Value=0) 140 6e-34 SB_8490| Best HMM Match : HSP70 (HMM E-Value=0) 140 6e-34 SB_56180| Best HMM Match : No HMM Matches (HMM E-Value=.) 133 8e-32 SB_45647| Best HMM Match : HSP70 (HMM E-Value=0) 121 4e-28 SB_57217| Best HMM Match : Helicase_C (HMM E-Value=0.049) 85 5e-17 SB_51590| Best HMM Match : HSP70 (HMM E-Value=4.4e-36) 73 2e-13 SB_15336| Best HMM Match : No HMM Matches (HMM E-Value=.) 62 4e-10 SB_31398| Best HMM Match : No HMM Matches (HMM E-Value=.) 52 3e-07 SB_19079| Best HMM Match : LCM (HMM E-Value=6.3e-37) 29 2.7 SB_58645| Best HMM Match : Kelch_1 (HMM E-Value=1.9) 29 2.7 SB_34478| Best HMM Match : No HMM Matches (HMM E-Value=.) 29 3.6 SB_7062| Best HMM Match : AMP-binding (HMM E-Value=1.4e-18) 29 3.6 SB_50521| Best HMM Match : MFS_1 (HMM E-Value=0.0026) 28 6.2 SB_29100| Best HMM Match : TPMT (HMM E-Value=5.4e-33) 27 8.2 SB_43817| Best HMM Match : WD40 (HMM E-Value=1e-13) 27 8.2 SB_17340| Best HMM Match : 7tm_3 (HMM E-Value=0) 27 8.2 SB_6273| Best HMM Match : MAM (HMM E-Value=0) 27 8.2 >SB_45540| Best HMM Match : HSP70 (HMM E-Value=0) Length = 1097 Score = 140 bits (340), Expect = 6e-34 Identities = 77/169 (45%), Positives = 94/169 (55%) Frame = +3 Query: 3 ARADTERLIGDAAKNQVAMNPNNTIFDAKRLIGRKFEDATVQADMKHWPFEVVSDGGKPK 182 A +DTERLIGDAAKNQVAMNP NT+FDAKRLIGRKF+D V DM HWPF V+ +G +PK Sbjct: 43 AFSDTERLIGDAAKNQVAMNPENTVFDAKRLIGRKFDDPVVARDMTHWPFHVIREGERPK 102 Query: 183 IKVAYKGEDKTFFPEEVSSMVLTK*RKLPKLISAKLCRMQLSRFPRTSMTLKDKPQKMQV 362 I+V +KGE K+FFPEE+SSMVLTK ++ + + P + + K Sbjct: 103 IQVEFKGEKKSFFPEEISSMVLTKMKETAEAYLGAKVTDAVVTVPAYFHDSQRQATKDAG 162 Query: 363 PSQA*TFSESSMNPXXXXXXXXXXXXXXENEMXLSXXLGGGTFDVSILT 509 P L LGGGTFDVS+LT Sbjct: 163 TIAGLNVLRIINEPTAAAIAYGLDKKVGVERNVLIYDLGGGTFDVSVLT 211 Score = 136 bits (328), Expect = 2e-32 Identities = 75/116 (64%), Positives = 84/116 (72%) Frame = +2 Query: 200 G*RQNLFPRGSQFHGAYEMKETAEAYLGKTVQNAVITVPAYFNDSQRQATKDAGTISGLN 379 G +++ FP +MKETAEAYLG V +AV+TVPAYF+DSQRQATKDAGTI+GLN Sbjct: 109 GEKKSFFPEEISSMVLTKMKETAEAYLGAKVTDAVVTVPAYFHDSQRQATKDAGTIAGLN 168 Query: 380 VLRIINEPTAAAIAYGLDKKGTGERNVLIFXPRRRYLRRVHPYIEDXIFXVKSTAG 547 VLRIINEPTAAAIAYGLDKK ERNVLI+ IED IF VKSTAG Sbjct: 169 VLRIINEPTAAAIAYGLDKKVGVERNVLIYDLGGGTFDVSVLTIEDGIFEVKSTAG 224 >SB_8490| Best HMM Match : HSP70 (HMM E-Value=0) Length = 640 Score = 140 bits (340), Expect = 6e-34 Identities = 77/166 (46%), Positives = 96/166 (57%) Frame = +3 Query: 12 DTERLIGDAAKNQVAMNPNNTIFDAKRLIGRKFEDATVQADMKHWPFEVVSDGGKPKIKV 191 DTERL+GDAAKNQVA+NP NTIFDAKRLIGR+++DATVQ+DMK WPF+++SD KPKI+V Sbjct: 46 DTERLVGDAAKNQVALNPENTIFDAKRLIGRRYDDATVQSDMKLWPFKIISDNNKPKIQV 105 Query: 192 AYKGEDKTFFPEEVSSMVLTK*RKLPKLISAKLCRMQLSRFPRTSMTLKDKPQKMQVPSQ 371 YKGE KTF EE+SSMVL K ++ + + + P + + K Sbjct: 106 EYKGERKTFAAEEISSMVLGKMKETAEAYLGQKVTSAVITVPAYFNDSQRQATKDAGTIA 165 Query: 372 A*TFSESSMNPXXXXXXXXXXXXXXENEMXLSXXLGGGTFDVSILT 509 P + L LGGGTFDVSILT Sbjct: 166 GLNVLRVINEPTAAALAYGLDKSLSGEKNVLIFDLGGGTFDVSILT 211 Score = 132 bits (318), Expect = 3e-31 Identities = 70/100 (70%), Positives = 80/100 (80%), Gaps = 1/100 (1%) Frame = +2 Query: 251 EMKETAEAYLGKTVQNAVITVPAYFNDSQRQATKDAGTISGLNVLRIINEPTAAAIAYGL 430 +MKETAEAYLG+ V +AVITVPAYFNDSQRQATKDAGTI+GLNVLR+INEPTAAA+AYGL Sbjct: 126 KMKETAEAYLGQKVTSAVITVPAYFNDSQRQATKDAGTIAGLNVLRVINEPTAAALAYGL 185 Query: 431 DKKGTGERNVLIFXPRRRYLRRVHPYIED-XIFXVKSTAG 547 DK +GE+NVLIF I+D +F VKSTAG Sbjct: 186 DKSLSGEKNVLIFDLGGGTFDVSILTIDDGSLFEVKSTAG 225 >SB_56180| Best HMM Match : No HMM Matches (HMM E-Value=.) Length = 746 Score = 133 bits (322), Expect = 8e-32 Identities = 73/99 (73%), Positives = 76/99 (76%) Frame = +2 Query: 251 EMKETAEAYLGKTVQNAVITVPAYFNDSQRQATKDAGTISGLNVLRIINEPTAAAIAYGL 430 +MKETAEAYLG V +AV+TVPAYFNDSQRQATKDAG ISGLNVLRIINEPTAAAIAYGL Sbjct: 219 KMKETAEAYLGCKVTDAVVTVPAYFNDSQRQATKDAGVISGLNVLRIINEPTAAAIAYGL 278 Query: 431 DKKGTGERNVLIFXPRRRYLRRVHPYIEDXIFXVKSTAG 547 DKK ERNVLIF IED IF VKST G Sbjct: 279 DKKVGQERNVLIFDLGGGTFDVSVLSIEDGIFEVKSTHG 317 Score = 126 bits (305), Expect = 1e-29 Identities = 57/77 (74%), Positives = 65/77 (84%) Frame = +3 Query: 3 ARADTERLIGDAAKNQVAMNPNNTIFDAKRLIGRKFEDATVQADMKHWPFEVVSDGGKPK 182 A D ERL+GDAAKNQVAMNP NTIFDAKRLIGR+F+D V+ DMKHW FEVV++ G+PK Sbjct: 43 AFTDEERLVGDAAKNQVAMNPTNTIFDAKRLIGRRFDDPGVKDDMKHWSFEVVNEAGRPK 102 Query: 183 IKVAYKGEDKTFFPEEV 233 +KV YKGE KTFF EEV Sbjct: 103 VKVEYKGETKTFFAEEV 119 Score = 66.9 bits (156), Expect = 1e-11 Identities = 31/37 (83%), Positives = 34/37 (91%) Frame = +2 Query: 251 EMKETAEAYLGKTVQNAVITVPAYFNDSQRQATKDAG 361 +MKETAEAYLG V +AV+TVPAYFNDSQRQATKDAG Sbjct: 151 KMKETAEAYLGCKVTDAVVTVPAYFNDSQRQATKDAG 187 >SB_45647| Best HMM Match : HSP70 (HMM E-Value=0) Length = 1327 Score = 121 bits (292), Expect = 4e-28 Identities = 57/73 (78%), Positives = 67/73 (91%) Frame = +2 Query: 251 EMKETAEAYLGKTVQNAVITVPAYFNDSQRQATKDAGTISGLNVLRIINEPTAAAIAYGL 430 +MKETAEAYLGK V +AV+TVPAYFND+QRQATKDAG I+GL V+RIINEPTAAAIAYG+ Sbjct: 824 KMKETAEAYLGKKVTHAVVTVPAYFNDAQRQATKDAGAIAGLTVMRIINEPTAAAIAYGM 883 Query: 431 DKKGTGERNVLIF 469 DKK GE+N+L+F Sbjct: 884 DKK-EGEKNILVF 895 Score = 111 bits (266), Expect = 5e-25 Identities = 66/164 (40%), Positives = 86/164 (52%) Frame = +3 Query: 18 ERLIGDAAKNQVAMNPNNTIFDAKRLIGRKFEDATVQADMKHWPFEVVSDGGKPKIKVAY 197 ERLIGD AKNQ+ NP NT+FDAKRLIGR ++D +VQ D+K +PF+VV KP ++V Sbjct: 746 ERLIGDGAKNQLTTNPENTVFDAKRLIGRSWDDTSVQNDLKFFPFKVVDKNKKPHVQVKV 805 Query: 198 KGEDKTFFPEEVSSMVLTK*RKLPKLISAKLCRMQLSRFPRTSMTLKDKPQKMQVPSQA* 377 K E KTF EE+S+MVLTK ++ + K + P + + K Sbjct: 806 KDEIKTFAAEEISAMVLTKMKETAEAYLGKKVTHAVVTVPAYFNDAQRQATKDAGAIAGL 865 Query: 378 TFSESSMNPXXXXXXXXXXXXXXENEMXLSXXLGGGTFDVSILT 509 T P E + L LGGGTFDVS+LT Sbjct: 866 TVMRIINEPTAAAIAYGMDKKEGEKNI-LVFDLGGGTFDVSLLT 908 >SB_57217| Best HMM Match : Helicase_C (HMM E-Value=0.049) Length = 179 Score = 84.6 bits (200), Expect = 5e-17 Identities = 53/95 (55%), Positives = 62/95 (65%), Gaps = 26/95 (27%) Frame = +2 Query: 251 EMKETAEAYLGKTVQNAVITVPAYFNDSQRQATKDAGTISGLN----------------- 379 ++K AEA+LG+ V+ AVITVPA+FND+QRQATKDAGTI+GL+ Sbjct: 25 QLKADAEAFLGQAVKRAVITVPAHFNDAQRQATKDAGTIAGLDVLSRTASERPPSRFRRR 84 Query: 380 -------VLRIINEPTAAAIAYGLDKK--GTGERN 457 VLRIINEPTAAAIAYGLDK G ERN Sbjct: 85 RPHGRSRVLRIINEPTAAAIAYGLDKARGGKAERN 119 >SB_51590| Best HMM Match : HSP70 (HMM E-Value=4.4e-36) Length = 437 Score = 72.5 bits (170), Expect = 2e-13 Identities = 37/72 (51%), Positives = 51/72 (70%) Frame = +2 Query: 251 EMKETAEAYLGKTVQNAVITVPAYFNDSQRQATKDAGTISGLNVLRIINEPTAAAIAYGL 430 +++ AE L TV AV++VPA F+ QR AT A +++G+ VLR+INEPTAAA+AYGL Sbjct: 156 KLRTMAERNLTTTVTKAVMSVPAEFDIKQRNATVKAASLAGITVLRVINEPTAAALAYGL 215 Query: 431 DKKGTGERNVLI 466 KK G NV++ Sbjct: 216 HKK-EGVANVMV 226 Score = 56.4 bits (130), Expect = 2e-08 Identities = 46/169 (27%), Positives = 67/169 (39%), Gaps = 1/169 (0%) Frame = +3 Query: 3 ARADTERLIGDAAKNQVAMNPNNTIFDAKRLIGRKFEDATVQADMKHWPFEVVSD-GGKP 179 A D L+G+ A +Q +NP NTI+DAKR IG+ F + K + F V +D G Sbjct: 72 AFTDKGVLVGEKALSQAEINPKNTIYDAKRFIGKTFTKQELLEIGKLYQFRVTTDKRGNA 131 Query: 180 KIKVAYKGEDKTFFPEEVSSMVLTK*RKLPKLISAKLCRMQLSRFPRTSMTLKDKPQKMQ 359 V KG PEE+ V+ K R + + + P +K + ++ Sbjct: 132 GFLVGKKGNFTVITPEEIGGYVIKKLRTMAERNLTTTVTKAVMSVP-AEFDIKQRNATVK 190 Query: 360 VPSQA*TFSESSMNPXXXXXXXXXXXXXXENEMXLSXXLGGGTFDVSIL 506 S A +N + LGGGT DVS+L Sbjct: 191 AASLAGITVLRVINEPTAAALAYGLHKKEGVANVMVVDLGGGTLDVSLL 239 >SB_15336| Best HMM Match : No HMM Matches (HMM E-Value=.) Length = 1068 Score = 61.7 bits (143), Expect = 4e-10 Identities = 28/62 (45%), Positives = 42/62 (67%) Frame = +2 Query: 254 MKETAEAYLGKTVQNAVITVPAYFNDSQRQATKDAGTISGLNVLRIINEPTAAAIAYGLD 433 +K TAE L V + VI+VP+Y+ D QR+ DA +GLN LR++N+ TA ++AYG+ Sbjct: 184 LKTTAEIALKTKVTDCVISVPSYYTDRQRRCMLDASATAGLNCLRLMNDTTAVSLAYGIY 243 Query: 434 KK 439 K+ Sbjct: 244 KQ 245 Score = 55.6 bits (128), Expect = 3e-08 Identities = 28/66 (42%), Positives = 39/66 (59%), Gaps = 1/66 (1%) Frame = +3 Query: 60 NPNNTIFDAKRLIGRKFEDATVQADMKHWPFEVVS-DGGKPKIKVAYKGEDKTFFPEEVS 236 N NTI KR IGRKF D VQ ++ H P++VV I+V Y G+ + F PE+V Sbjct: 118 NLKNTISQFKRFIGRKFSDPAVQKEIPHLPYKVVELPNDSIGIQVQYLGKQEVFSPEQVM 177 Query: 237 SMVLTK 254 +M+ T+ Sbjct: 178 AMLFTR 183 >SB_31398| Best HMM Match : No HMM Matches (HMM E-Value=.) Length = 1019 Score = 52.0 bits (119), Expect = 3e-07 Identities = 23/54 (42%), Positives = 36/54 (66%) Frame = +2 Query: 269 EAYLGKTVQNAVITVPAYFNDSQRQATKDAGTISGLNVLRIINEPTAAAIAYGL 430 E + +++ V+TVP +FN ++R+A A + GLNVL+I+N TA A+ YGL Sbjct: 98 EQFADHPIKDVVLTVPPFFNQAERRALLRAAELVGLNVLQIMNSNTAVALNYGL 151 >SB_19079| Best HMM Match : LCM (HMM E-Value=6.3e-37) Length = 420 Score = 29.1 bits (62), Expect = 2.7 Identities = 9/16 (56%), Positives = 11/16 (68%) Frame = -1 Query: 81 RILCCWGSSPPGSWRH 34 R++CC G SP WRH Sbjct: 395 RVVCCQGDSPSPRWRH 410 >SB_58645| Best HMM Match : Kelch_1 (HMM E-Value=1.9) Length = 340 Score = 29.1 bits (62), Expect = 2.7 Identities = 9/16 (56%), Positives = 11/16 (68%) Frame = -1 Query: 81 RILCCWGSSPPGSWRH 34 R++CC G SP WRH Sbjct: 170 RVVCCQGDSPSPRWRH 185 >SB_34478| Best HMM Match : No HMM Matches (HMM E-Value=.) Length = 133 Score = 28.7 bits (61), Expect = 3.6 Identities = 22/63 (34%), Positives = 28/63 (44%), Gaps = 14/63 (22%) Frame = +2 Query: 8 SRHRASHRRCRQEPGGDEP----QQ----------HNIRCQTSHRT*VRRCYCASRHEAL 145 S R S C++ PG D+P QQ H++RC+ HR VRR Y RH Sbjct: 10 SSRRRSQLVCKRMPGADKPVRARQQFFAEQIVLRVHHMRCEIPHRC-VRRKYRIRRHSPF 68 Query: 146 AFR 154 R Sbjct: 69 RLR 71 >SB_7062| Best HMM Match : AMP-binding (HMM E-Value=1.4e-18) Length = 817 Score = 28.7 bits (61), Expect = 3.6 Identities = 18/59 (30%), Positives = 31/59 (52%) Frame = +2 Query: 209 QNLFPRGSQFHGAYEMKETAEAYLGKTVQNAVITVPAYFNDSQRQATKDAGTISGLNVL 385 + L+ +Q H +E KE E + T N+ +T+P ++ Q TKD T +G ++L Sbjct: 561 RTLYGSSAQSH-LFEDKEKGEDH-NMTSGNSAVTLPGTQAETDGQLTKDMNTQAGPSML 617 >SB_50521| Best HMM Match : MFS_1 (HMM E-Value=0.0026) Length = 1080 Score = 27.9 bits (59), Expect = 6.2 Identities = 16/53 (30%), Positives = 28/53 (52%) Frame = +2 Query: 290 VQNAVITVPAYFNDSQRQATKDAGTISGLNVLRIINEPTAAAIAYGLDKKGTG 448 + NA +T+P YF + +K G G+ + +++ I+Y LD+K TG Sbjct: 1020 IYNAPLTLPPYFWCHPSRKSKKDGAKEGMAM--AMSKERNQEISYKLDRKWTG 1070 >SB_29100| Best HMM Match : TPMT (HMM E-Value=5.4e-33) Length = 242 Score = 27.5 bits (58), Expect = 8.2 Identities = 14/38 (36%), Positives = 21/38 (55%), Gaps = 2/38 (5%) Frame = +2 Query: 410 AAIAYGLDKKGTGERNVLI--FXPRRRYLRRVHPYIED 517 +AI +D +G+ N+L+ P RY+ H YIED Sbjct: 150 SAIGLYIDNRGSRYANLLVSLLKPHGRYMIENHHYIED 187 >SB_43817| Best HMM Match : WD40 (HMM E-Value=1e-13) Length = 822 Score = 27.5 bits (58), Expect = 8.2 Identities = 23/71 (32%), Positives = 33/71 (46%), Gaps = 1/71 (1%) Frame = -3 Query: 250 VSTMELTSSGKKVLSSPLYATLILGLPPSLTTSKGQCFMS-ACTVASSNLRPMRRLASNI 74 VST+++T G +L+ T LG+ + T S S T+ S +L PMRR + Sbjct: 751 VSTVDVTLDGLYILAGT--TTGNLGVLNASTRSYTTLMRSHTSTITSMDLDPMRRHMGTV 808 Query: 73 VLLGFIATWFL 41 G I W L Sbjct: 809 SADGTIRIWDL 819 >SB_17340| Best HMM Match : 7tm_3 (HMM E-Value=0) Length = 890 Score = 27.5 bits (58), Expect = 8.2 Identities = 17/50 (34%), Positives = 25/50 (50%), Gaps = 1/50 (2%) Frame = -3 Query: 295 LHSFAEISFGSFLHFVSTMELTSSG-KKVLSSPLYATLILGLPPSLTTSK 149 L S A + +FL V + +S + LSSPLY T +PP + +K Sbjct: 212 LTSRAALPLANFLQAVRIPLVDASATSEELSSPLYKTFFRTIPPDVNQAK 261 >SB_6273| Best HMM Match : MAM (HMM E-Value=0) Length = 4272 Score = 27.5 bits (58), Expect = 8.2 Identities = 9/27 (33%), Positives = 16/27 (59%) Frame = +1 Query: 232 SVPWCLRNEGNCRSLSRQNCAECSYHG 312 +VPWC + +C S++ Q +C + G Sbjct: 1317 NVPWCFYHPSSCSSINPQLRRDCGWVG 1343 Database: nematostella Posted date: Oct 22, 2007 1:22 PM Number of letters in database: 16,821,457 Number of sequences in database: 59,808 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 18,412,096 Number of Sequences: 59808 Number of extensions: 374438 Number of successful extensions: 1047 Number of sequences better than 10.0: 17 Number of HSP's better than 10.0 without gapping: 987 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 1042 length of database: 16,821,457 effective HSP length: 78 effective length of database: 12,156,433 effective search space used: 1361520496 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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