Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= NV060403.seq
(567 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPCC794.09c |ef1a-a||translation elongation factor EF-1 alpha Ef... 163 2e-41
SPAC23A1.10 |ef1a-b||translation elongation factor EF-1 alpha Ef... 162 3e-41
SPBC839.15c |ef1a-c||translation elongation factor EF-1 alpha Ef... 162 3e-41
SPBC25B2.01 ||SPBC2G5.08|elongation factor 1 alpha related prote... 96 4e-21
SPCC584.04 |sup35|erf3|translation release factor eRF3 |Schizosa... 96 4e-21
SPBC9B6.04c |tuf1||mitochondrial translation elongation factor E... 76 3e-15
SPBC1306.01c ||SPBC409.22c|translation elongation factor G|Schiz... 37 0.002
SPCP31B10.07 |eft202||translation elongation factor 2 |Schizosac... 36 0.005
SPAC513.01c |eft201|eft2-1, etf2, SPAPYUK71.04c|translation elon... 36 0.005
SPBC660.10 |||translation elongation factor G|Schizosaccharomyce... 35 0.010
SPBC17G9.09 |tif213||translation initiation factor eIF2 gamma su... 34 0.013
SPBC1271.15c |||translation initiation factor IF-2Mt|Schizosacch... 34 0.017
SPAC1B3.04c |||mitochondrial GTPase Guf1 |Schizosaccharomyces po... 31 0.16
SPCC553.08c |||GTPase Ria1 |Schizosaccharomyces pombe|chr 3|||Ma... 30 0.21
SPAC56F8.03 |||translation initiation factor IF2 |Schizosaccharo... 29 0.36
SPBC215.12 |cwf10|spef2, snu114|GTPase Cwf10 |Schizosaccharomyce... 29 0.48
SPBC1703.06 |pof10||F-box protein Pof10|Schizosaccharomyces pomb... 28 0.83
SPAC9G1.11c |spn4||septin Spn4|Schizosaccharomyces pombe|chr 1||... 26 3.4
SPAC607.08c |||DUF726 family protein|Schizosaccharomyces pombe|c... 26 3.4
SPBC1703.13c |||inorganic phosphate transporter |Schizosaccharom... 25 7.7
>SPCC794.09c |ef1a-a||translation elongation factor EF-1 alpha
Ef1a-a |Schizosaccharomyces pombe|chr 3|||Manual
Length = 460
Score = 163 bits (395), Expect = 2e-41
Identities = 76/85 (89%), Positives = 80/85 (94%)
Frame = +1
Query: 256 GITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISK 435
GITIDIALWKFET KY VT+IDAPGHRDFIKNMITGTSQADCAVLI+ GTGEFEAGISK
Sbjct: 70 GITIDIALWKFETPKYNVTVIDAPGHRDFIKNMITGTSQADCAVLIIGGGTGEFEAGISK 129
Query: 436 NGQTREHALLAFTLGVKQLIVGVNK 510
+GQTREHALLA+TLGVKQLIV VNK
Sbjct: 130 DGQTREHALLAYTLGVKQLIVAVNK 154
Score = 138 bits (335), Expect = 4e-34
Identities = 63/68 (92%), Positives = 65/68 (95%)
Frame = +3
Query: 51 MGKEKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEXEAQEMGKGSFKYAWVL 230
MGKEK HIN+VVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFE EA E+GKGSFKYAWVL
Sbjct: 1 MGKEKGHINVVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEATELGKGSFKYAWVL 60
Query: 231 DKLKAERE 254
DKLKAERE
Sbjct: 61 DKLKAERE 68
>SPAC23A1.10 |ef1a-b||translation elongation factor EF-1 alpha
Ef1a-b |Schizosaccharomyces pombe|chr 1|||Manual
Length = 460
Score = 162 bits (394), Expect = 3e-41
Identities = 75/85 (88%), Positives = 80/85 (94%)
Frame = +1
Query: 256 GITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISK 435
GITIDIALWKFET KY VT+IDAPGHRDFIKNMITGTSQADCA+LI+ GTGEFEAGISK
Sbjct: 70 GITIDIALWKFETPKYNVTVIDAPGHRDFIKNMITGTSQADCAILIIGGGTGEFEAGISK 129
Query: 436 NGQTREHALLAFTLGVKQLIVGVNK 510
+GQTREHALLA+TLGVKQLIV VNK
Sbjct: 130 DGQTREHALLAYTLGVKQLIVAVNK 154
Score = 138 bits (335), Expect = 4e-34
Identities = 63/68 (92%), Positives = 65/68 (95%)
Frame = +3
Query: 51 MGKEKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEXEAQEMGKGSFKYAWVL 230
MGKEK HIN+VVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFE EA E+GKGSFKYAWVL
Sbjct: 1 MGKEKGHINVVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEATELGKGSFKYAWVL 60
Query: 231 DKLKAERE 254
DKLKAERE
Sbjct: 61 DKLKAERE 68
>SPBC839.15c |ef1a-c||translation elongation factor EF-1 alpha
Ef1a-c |Schizosaccharomyces pombe|chr 2|||Manual
Length = 460
Score = 162 bits (394), Expect = 3e-41
Identities = 75/85 (88%), Positives = 80/85 (94%)
Frame = +1
Query: 256 GITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISK 435
GITIDIALWKFET KY VT+IDAPGHRDFIKNMITGTSQADCA+LI+ GTGEFEAGISK
Sbjct: 70 GITIDIALWKFETPKYNVTVIDAPGHRDFIKNMITGTSQADCAILIIGGGTGEFEAGISK 129
Query: 436 NGQTREHALLAFTLGVKQLIVGVNK 510
+GQTREHALLA+TLGVKQLIV VNK
Sbjct: 130 DGQTREHALLAYTLGVKQLIVAVNK 154
Score = 138 bits (335), Expect = 4e-34
Identities = 63/68 (92%), Positives = 65/68 (95%)
Frame = +3
Query: 51 MGKEKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEXEAQEMGKGSFKYAWVL 230
MGKEK HIN+VVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFE EA E+GKGSFKYAWVL
Sbjct: 1 MGKEKGHINVVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEATELGKGSFKYAWVL 60
Query: 231 DKLKAERE 254
DKLKAERE
Sbjct: 61 DKLKAERE 68
>SPBC25B2.01 ||SPBC2G5.08|elongation factor 1 alpha related
protein|Schizosaccharomyces pombe|chr 2|||Manual
Length = 592
Score = 95.9 bits (228), Expect = 4e-21
Identities = 45/85 (52%), Positives = 56/85 (65%)
Frame = +1
Query: 256 GITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISK 435
G+T+D+A FE+ K I DAPGHRDFI MI G S AD AVL+V + FE G +
Sbjct: 240 GVTMDVASTTFESDKKIYEIGDAPGHRDFISGMIAGASSADFAVLVVDSSQNNFERGFLE 299
Query: 436 NGQTREHALLAFTLGVKQLIVGVNK 510
NGQTREHA L LG+ +++V VNK
Sbjct: 300 NGQTREHAYLLRALGISEIVVSVNK 324
Score = 72.1 bits (169), Expect = 5e-14
Identities = 31/63 (49%), Positives = 44/63 (69%)
Frame = +3
Query: 63 KTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEXEAQEMGKGSFKYAWVLDKLK 242
K +++VV GHVDSGKST G ++++ G I+ R+++K EA GKGSF YAW+LD +
Sbjct: 175 KPVVHLVVTGHVDSGKSTMLGRIMFELGEINSRSMQKLHNEAANSGKGSFSYAWLLDTTE 234
Query: 243 AER 251
ER
Sbjct: 235 EER 237
>SPCC584.04 |sup35|erf3|translation release factor eRF3
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 662
Score = 95.9 bits (228), Expect = 4e-21
Identities = 42/85 (49%), Positives = 59/85 (69%)
Frame = +1
Query: 256 GITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISK 435
G T+++ FET +++DAPGH+ ++ NMI G SQAD VL+++A GEFEAG +
Sbjct: 301 GKTVEVGRAYFETEHRRFSLLDAPGHKGYVTNMINGASQADIGVLVISARRGEFEAGFER 360
Query: 436 NGQTREHALLAFTLGVKQLIVGVNK 510
GQTREHA+LA T G+ L+V +NK
Sbjct: 361 GGQTREHAVLARTQGINHLVVVINK 385
Score = 79.4 bits (187), Expect = 3e-16
Identities = 35/64 (54%), Positives = 46/64 (71%)
Frame = +3
Query: 63 KTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEXEAQEMGKGSFKYAWVLDKLK 242
K H+NIV IGHVD+GKST G++++ G +DKRT+EK E EA+E GK S+ +W LD
Sbjct: 236 KEHVNIVFIGHVDAGKSTLGGNILFLTGMVDKRTMEKIEREAKEAGKESWYLSWALDSTS 295
Query: 243 AERE 254
ERE
Sbjct: 296 EERE 299
>SPBC9B6.04c |tuf1||mitochondrial translation elongation factor
EF-Tu Tuf1 |Schizosaccharomyces pombe|chr 2|||Manual
Length = 439
Score = 76.2 bits (179), Expect = 3e-15
Identities = 39/85 (45%), Positives = 55/85 (64%)
Frame = +1
Query: 256 GITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISK 435
GITI A ++ET+ + +D PGH D+IKNMITG + D A+++V+A G+
Sbjct: 101 GITISSAHVEYETANRHYAHVDCPGHADYIKNMITGAATMDGAIIVVSATDGQMP----- 155
Query: 436 NGQTREHALLAFTLGVKQLIVGVNK 510
QTREH LLA +GVKQ++V +NK
Sbjct: 156 --QTREHLLLARQVGVKQIVVYINK 178
Score = 35.1 bits (77), Expect = 0.007
Identities = 16/29 (55%), Positives = 20/29 (68%)
Frame = +3
Query: 57 KEKTHINIVVIGHVDSGKSTTTGHLIYKC 143
++K H+NI IGHVD GK+T T I KC
Sbjct: 49 RKKPHVNIGTIGHVDHGKTTLTA-AITKC 76
>SPBC1306.01c ||SPBC409.22c|translation elongation factor
G|Schizosaccharomyces pombe|chr 2|||Manual
Length = 770
Score = 36.7 bits (81), Expect = 0.002
Identities = 24/76 (31%), Positives = 36/76 (47%)
Frame = +1
Query: 286 FETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNGQTREHALL 465
FE S Y + IID PGH DF + D AVL++ A +G I+ + Q R + +
Sbjct: 141 FEKS-YNINIIDTPGHIDFTIEVERALRVLDGAVLVLCAVSGVQSQTITVDRQMRRYNVP 199
Query: 466 AFTLGVKQLIVGVNKW 513
+ K +G + W
Sbjct: 200 RISFVNKMDRMGADPW 215
Score = 29.1 bits (62), Expect = 0.48
Identities = 12/26 (46%), Positives = 17/26 (65%)
Frame = +3
Query: 75 NIVVIGHVDSGKSTTTGHLIYKCGGI 152
NI + H+DSGK+T T ++Y G I
Sbjct: 61 NIGISAHIDSGKTTFTERVLYYTGRI 86
>SPCP31B10.07 |eft202||translation elongation factor 2
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 842
Score = 35.5 bits (78), Expect = 0.005
Identities = 17/32 (53%), Positives = 19/32 (59%)
Frame = +3
Query: 51 MGKEKTHINIVVIGHVDSGKSTTTGHLIYKCG 146
MGK N+ VI HVD GKST T L+ K G
Sbjct: 13 MGKPSNVRNMSVIAHVDHGKSTLTDSLVQKAG 44
Score = 27.9 bits (59), Expect = 1.1
Identities = 11/32 (34%), Positives = 18/32 (56%)
Frame = +1
Query: 301 YYVTIIDAPGHRDFIKNMITGTSQADCAVLIV 396
+ V +ID+PGH DF + D A+++V
Sbjct: 98 FLVNLIDSPGHVDFSSEVTAALRVTDGALVVV 129
>SPAC513.01c |eft201|eft2-1, etf2, SPAPYUK71.04c|translation
elongation factor 2 |Schizosaccharomyces pombe|chr
1|||Manual
Length = 842
Score = 35.5 bits (78), Expect = 0.005
Identities = 17/32 (53%), Positives = 19/32 (59%)
Frame = +3
Query: 51 MGKEKTHINIVVIGHVDSGKSTTTGHLIYKCG 146
MGK N+ VI HVD GKST T L+ K G
Sbjct: 13 MGKPSNVRNMSVIAHVDHGKSTLTDSLVQKAG 44
Score = 27.9 bits (59), Expect = 1.1
Identities = 11/32 (34%), Positives = 18/32 (56%)
Frame = +1
Query: 301 YYVTIIDAPGHRDFIKNMITGTSQADCAVLIV 396
+ V +ID+PGH DF + D A+++V
Sbjct: 98 FLVNLIDSPGHVDFSSEVTAALRVTDGALVVV 129
>SPBC660.10 |||translation elongation factor G|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 813
Score = 34.7 bits (76), Expect = 0.010
Identities = 18/52 (34%), Positives = 24/52 (46%)
Frame = +1
Query: 256 GITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTG 411
GITI+ A F + +ID PGH DF + + D AV I+ G
Sbjct: 78 GITINSAAISFTWRNQRINLIDTPGHADFTFEVERSVAVLDGAVAIIDGSAG 129
Score = 28.7 bits (61), Expect = 0.63
Identities = 10/24 (41%), Positives = 17/24 (70%)
Frame = +3
Query: 75 NIVVIGHVDSGKSTTTGHLIYKCG 146
N+ +I H+D+GK+T T ++Y G
Sbjct: 30 NVGIIAHIDAGKTTLTEKMLYYGG 53
>SPBC17G9.09 |tif213||translation initiation factor eIF2 gamma
subunit|Schizosaccharomyces pombe|chr 2|||Manual
Length = 446
Score = 34.3 bits (75), Expect = 0.013
Identities = 19/69 (27%), Positives = 32/69 (46%)
Frame = +1
Query: 304 YVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNGQTREHALLAFTLGV 483
+V+ +D PGH + M+ G + D A+L++A QT EH + +
Sbjct: 109 HVSFVDCPGHDILMATMLNGAAVMDAALLLIAGNES------CPQPQTSEHLAAIEIMQL 162
Query: 484 KQLIVGVNK 510
K +I+ NK
Sbjct: 163 KHIIILQNK 171
Score = 25.0 bits (52), Expect = 7.7
Identities = 12/24 (50%), Positives = 14/24 (58%)
Frame = +3
Query: 45 PKMGKEKTHINIVVIGHVDSGKST 116
P + + INI IGHV GKST
Sbjct: 15 PAIISRQATINIGTIGHVAHGKST 38
>SPBC1271.15c |||translation initiation factor
IF-2Mt|Schizosaccharomyces pombe|chr 2|||Manual
Length = 686
Score = 33.9 bits (74), Expect = 0.017
Identities = 19/54 (35%), Positives = 26/54 (48%), Gaps = 2/54 (3%)
Frame = +1
Query: 256 GITIDIALWK--FETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTG 411
GIT I + F+ ++T +D PGH F G + AD VL+VA G
Sbjct: 203 GITQKIGAFTVPFDKGSKFITFLDTPGHMAFEAMRKRGANIADIVVLVVAGDDG 256
>SPAC1B3.04c |||mitochondrial GTPase Guf1 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 646
Score = 30.7 bits (66), Expect = 0.16
Identities = 12/45 (26%), Positives = 23/45 (51%)
Frame = +1
Query: 277 LWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTG 411
++ + Y + +ID PGH DF ++ + + +L+V A G
Sbjct: 116 IYYYHGQSYLLNLIDTPGHVDFRAEVMHSLAACEGCILLVDASQG 160
>SPCC553.08c |||GTPase Ria1 |Schizosaccharomyces pombe|chr
3|||Manual
Length = 1000
Score = 30.3 bits (65), Expect = 0.21
Identities = 13/37 (35%), Positives = 21/37 (56%)
Frame = +1
Query: 301 YYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTG 411
Y + +ID+PGH DF + + + D A ++V A G
Sbjct: 94 YLINLIDSPGHVDFSSEVSSASRLCDGAFVLVDAVEG 130
>SPAC56F8.03 |||translation initiation factor IF2
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1079
Score = 29.5 bits (63), Expect = 0.36
Identities = 12/28 (42%), Positives = 16/28 (57%)
Frame = +1
Query: 313 IIDAPGHRDFIKNMITGTSQADCAVLIV 396
IID PGH F GTS + A+L++
Sbjct: 553 IIDTPGHESFTNLRSRGTSLCNIAILVI 580
>SPBC215.12 |cwf10|spef2, snu114|GTPase Cwf10 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 983
Score = 29.1 bits (62), Expect = 0.48
Identities = 13/51 (25%), Positives = 25/51 (49%)
Frame = +1
Query: 244 LSVXGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIV 396
+S+ + +A+ + + ID PGH DF+ + + +D VL+V
Sbjct: 190 MSIKSTPLTLAVSDMKGKTFAFQCIDTPGHVDFVDEVAAPMAISDGVVLVV 240
>SPBC1703.06 |pof10||F-box protein Pof10|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 662
Score = 28.3 bits (60), Expect = 0.83
Identities = 19/62 (30%), Positives = 25/62 (40%)
Frame = -1
Query: 294 SFELPESNIDCDTXHAQPLVCPIPKHI*RILYPFPGPPSRTSRWYVCQYHHICRSSDQWW 115
S L N D + + V +PK I I++ F P S S C+Y S D W
Sbjct: 12 SSNLRRMNRDHSSNNTNRTVLNLPKEILIIIFSFLDPRSLLSAQCTCKYWKKLLSDDLSW 71
Query: 114 WT 109
T
Sbjct: 72 RT 73
>SPAC9G1.11c |spn4||septin Spn4|Schizosaccharomyces pombe|chr
1|||Manual
Length = 380
Score = 26.2 bits (55), Expect = 3.4
Identities = 12/33 (36%), Positives = 19/33 (57%), Gaps = 2/33 (6%)
Frame = +1
Query: 259 ITIDIALWKFETSKYYV--TIIDAPGHRDFIKN 351
+ I+I + E +++ T+ID PG DFI N
Sbjct: 71 VEIEITKAELEEKNFHLRLTVIDTPGFGDFINN 103
>SPAC607.08c |||DUF726 family protein|Schizosaccharomyces pombe|chr
1|||Manual
Length = 579
Score = 26.2 bits (55), Expect = 3.4
Identities = 16/61 (26%), Positives = 30/61 (49%)
Frame = +3
Query: 84 VIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEXEAQEMGKGSFKYAWVLDKLKAEREXYH 263
++ V+S +ST G +D T+E + ++ +G +Y W++ K+ ER Y
Sbjct: 460 LVYRVNSAQSTAAG---LGPVSLDSNTLENVD--VGDLVEGHLQYRWLVAKILKERLGYD 514
Query: 264 N 266
N
Sbjct: 515 N 515
>SPBC1703.13c |||inorganic phosphate transporter
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 311
Score = 25.0 bits (52), Expect = 7.7
Identities = 8/19 (42%), Positives = 12/19 (63%)
Frame = -1
Query: 192 PGPPSRTSRWYVCQYHHIC 136
P PP +T + Y QY+ +C
Sbjct: 8 PAPPKKTLQLYTPQYYGLC 26
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,264,677
Number of Sequences: 5004
Number of extensions: 44758
Number of successful extensions: 167
Number of sequences better than 10.0: 20
Number of HSP's better than 10.0 without gapping: 148
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 166
length of database: 2,362,478
effective HSP length: 69
effective length of database: 2,017,202
effective search space used: 240047038
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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