Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= NV060398.seq
(661 letters)
Database: mosquito
2352 sequences; 563,979 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
L07880-1|AAA29358.1| 218|Anopheles gambiae glutathione S-transf... 64 5e-12
AF513639-1|AAM53611.1| 195|Anopheles gambiae glutathione S-tran... 62 2e-11
AY063776-1|AAL59658.1| 224|Anopheles gambiae glutathione S-tran... 25 1.6
AF316635-1|AAG45163.1| 224|Anopheles gambiae glutathione S-tran... 25 1.6
Z81291-1|CAB03592.1| 209|Anopheles gambiae GSTD1-5 protein prot... 24 3.7
AF071160-3|AAC79993.1| 209|Anopheles gambiae glutathione S-tran... 24 3.7
AF515521-1|AAM61888.1| 233|Anopheles gambiae glutathione S-tran... 24 4.9
CR954257-11|CAJ14162.1| 415|Anopheles gambiae predicted protein... 23 6.5
AY170874-1|AAO34131.1| 1221|Anopheles gambiae alkali metal ion/p... 23 8.5
>L07880-1|AAA29358.1| 218|Anopheles gambiae glutathione
S-transferase protein.
Length = 218
Score = 63.7 bits (148), Expect = 5e-12
Identities = 33/87 (37%), Positives = 44/87 (50%)
Frame = +1
Query: 244 VRSEPVLEIDGKQYAQSTXICRYLGRKYGLAGANDEEAFEIDQNVEFLNDIRASAASVHY 423
+R PVLE+DGK+ QS +CRY+ ++ LAG N EA +ID V+ +ND R A V Y
Sbjct: 63 MRQMPVLEVDGKRVHQSLAMCRYVAKQINLAGDNPLEALQIDAIVDTINDFRLKIAIVAY 122
Query: 424 XXXXXXXXXXXXXXXXXXYPFFFEKLN 504
PF+ KLN
Sbjct: 123 EPDDMVKEKKMVTLNNEVIPFYLTKLN 149
Score = 62.5 bits (145), Expect = 1e-11
Identities = 28/50 (56%), Positives = 32/50 (64%)
Frame = +2
Query: 104 MPNVKFYYFPVKALGESQRLLLAYGGQEFEDNRISSENWPEFKPKTPFGQ 253
MP+ K YYF VKALGE R LL+YG F+D RI+ E WP KP P Q
Sbjct: 16 MPDYKVYYFNVKALGEPLRFLLSYGNLPFDDVRITREEWPALKPTMPMRQ 65
Score = 41.1 bits (92), Expect = 3e-05
Identities = 20/43 (46%), Positives = 24/43 (55%)
Frame = +3
Query: 516 KNNGHIALGKLTWGDFVYAGMYDYLXAMLQKPGSGAEVPGLXE 644
+NNGH+ LGK TW D +AG+ DYL L K P L E
Sbjct: 154 ENNGHLVLGKPTWADVYFAGILDYLN-YLTKTNLLENFPNLQE 195
>AF513639-1|AAM53611.1| 195|Anopheles gambiae glutathione
S-transferase S1-2 protein.
Length = 195
Score = 61.7 bits (143), Expect = 2e-11
Identities = 33/84 (39%), Positives = 43/84 (51%)
Frame = +1
Query: 256 PVLEIDGKQYAQSTXICRYLGRKYGLAGANDEEAFEIDQNVEFLNDIRASAASVHYXXXX 435
PVLE+DGK+ QS + RYL + GLAGA+D E ID V+ +ND R A V Y
Sbjct: 44 PVLEVDGKKVHQSVAMSRYLANQVGLAGADDWENLMIDTVVDTVNDFRLKIAVVSYEPDD 103
Query: 436 XXXXXXXXXXXXXXYPFFFEKLNE 507
PF+ EKL++
Sbjct: 104 EIKEKKLVTLNNEVIPFYLEKLDD 127
Score = 53.6 bits (123), Expect = 5e-09
Identities = 24/42 (57%), Positives = 27/42 (64%)
Frame = +2
Query: 128 FPVKALGESQRLLLAYGGQEFEDNRISSENWPEFKPKTPFGQ 253
F VKALGE R LL+YG F+D RI+ E WP KP P GQ
Sbjct: 1 FNVKALGEPLRFLLSYGNLPFDDVRITREEWPALKPTMPMGQ 42
Score = 32.7 bits (71), Expect = 0.011
Identities = 12/27 (44%), Positives = 17/27 (62%)
Frame = +3
Query: 519 NNGHIALGKLTWGDFVYAGMYDYLXAM 599
NNG++A KL+W D + + DYL M
Sbjct: 132 NNGYLANSKLSWADIYFTAILDYLNYM 158
>AY063776-1|AAL59658.1| 224|Anopheles gambiae glutathione
S-transferase E1 protein.
Length = 224
Score = 25.4 bits (53), Expect = 1.6
Identities = 13/31 (41%), Positives = 17/31 (54%)
Frame = +1
Query: 256 PVLEIDGKQYAQSTXICRYLGRKYGLAGAND 348
PVL+ +G ++S I YL RKYG D
Sbjct: 56 PVLDDNGTIISESHAIMIYLVRKYGQGEGKD 86
>AF316635-1|AAG45163.1| 224|Anopheles gambiae glutathione
S-transferase E1 protein.
Length = 224
Score = 25.4 bits (53), Expect = 1.6
Identities = 13/31 (41%), Positives = 17/31 (54%)
Frame = +1
Query: 256 PVLEIDGKQYAQSTXICRYLGRKYGLAGAND 348
PVL+ +G ++S I YL RKYG D
Sbjct: 56 PVLDDNGTIISESHAIMIYLVRKYGQGEGKD 86
>Z81291-1|CAB03592.1| 209|Anopheles gambiae GSTD1-5 protein
protein.
Length = 209
Score = 24.2 bits (50), Expect = 3.7
Identities = 11/25 (44%), Positives = 13/25 (52%)
Frame = +1
Query: 256 PVLEIDGKQYAQSTXICRYLGRKYG 330
P L +G +S IC YL KYG
Sbjct: 53 PTLVDNGFALWESRAICTYLAEKYG 77
>AF071160-3|AAC79993.1| 209|Anopheles gambiae glutathione
S-transferase protein.
Length = 209
Score = 24.2 bits (50), Expect = 3.7
Identities = 11/25 (44%), Positives = 13/25 (52%)
Frame = +1
Query: 256 PVLEIDGKQYAQSTXICRYLGRKYG 330
P L +G +S IC YL KYG
Sbjct: 53 PTLVDNGFALWESRAICTYLAEKYG 77
>AF515521-1|AAM61888.1| 233|Anopheles gambiae glutathione
S-transferase u1 protein.
Length = 233
Score = 23.8 bits (49), Expect = 4.9
Identities = 11/24 (45%), Positives = 15/24 (62%)
Frame = +1
Query: 256 PVLEIDGKQYAQSTXICRYLGRKY 327
PVL+ DG ++S I +YL KY
Sbjct: 53 PVLDDDGFFLSESNAILQYLCEKY 76
>CR954257-11|CAJ14162.1| 415|Anopheles gambiae predicted protein
protein.
Length = 415
Score = 23.4 bits (48), Expect = 6.5
Identities = 11/23 (47%), Positives = 15/23 (65%)
Frame = +2
Query: 152 SQRLLLAYGGQEFEDNRISSENW 220
S R L Y E+E +RIS+EN+
Sbjct: 357 SYRTKLQYQKHEYEVHRISNENF 379
>AY170874-1|AAO34131.1| 1221|Anopheles gambiae alkali metal
ion/proton exchanger 3 protein.
Length = 1221
Score = 23.0 bits (47), Expect = 8.5
Identities = 10/22 (45%), Positives = 13/22 (59%)
Frame = +2
Query: 62 VLLNNYSLLHNNPTMPNVKFYY 127
VLL + LH +P PN F+Y
Sbjct: 288 VLLRYATNLHVSPLTPNTFFFY 309
Database: mosquito
Posted date: Oct 23, 2007 1:18 PM
Number of letters in database: 563,979
Number of sequences in database: 2352
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 566,422
Number of Sequences: 2352
Number of extensions: 9288
Number of successful extensions: 32
Number of sequences better than 10.0: 9
Number of HSP's better than 10.0 without gapping: 27
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 32
length of database: 563,979
effective HSP length: 62
effective length of database: 418,155
effective search space used: 65650335
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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