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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= NV060394.seq
         (558 letters)

Database: uniref50 
           1,657,284 sequences; 575,637,011 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

UniRef50_Q8CF78 Cluster: Enolase; n=4; Murinae|Rep: Enolase - Mu...    94   2e-18
UniRef50_P13929 Cluster: Beta-enolase; n=32; Fungi/Metazoa group...    93   4e-18
UniRef50_UPI000066089D Cluster: Gamma-enolase (EC 4.2.1.11) (2-p...    91   2e-17
UniRef50_A7PGJ9 Cluster: Chromosome chr17 scaffold_16, whole gen...    91   2e-17
UniRef50_Q0HL72 Cluster: Enolase; n=126; Bacteria|Rep: Enolase -...    82   7e-15
UniRef50_Q05524 Cluster: Alpha-enolase, lung specific; n=613; ro...    82   7e-15
UniRef50_Q27727 Cluster: Enolase; n=72; cellular organisms|Rep: ...    81   2e-14
UniRef50_Q7NAY0 Cluster: Enolase; n=71; cellular organisms|Rep: ...    81   2e-14
UniRef50_Q8KG25 Cluster: Enolase 2; n=22; Bacteria|Rep: Enolase ...    81   2e-14
UniRef50_A4H7T5 Cluster: Enolase; n=2; cellular organisms|Rep: E...    80   4e-14
UniRef50_Q6F0Z7 Cluster: Enolase; n=349; cellular organisms|Rep:...    79   5e-14
UniRef50_A5DEC9 Cluster: Enolase; n=1; Pichia guilliermondii|Rep...    78   1e-13
UniRef50_Q7VQH3 Cluster: Enolase; n=9; Bacteria|Rep: Enolase - B...    76   6e-13
UniRef50_A2A756 Cluster: Enolase 1, alpha non-neuron; n=3; Euthe...    75   1e-12
UniRef50_Q9PQV9 Cluster: Enolase; n=1; Ureaplasma parvum|Rep: En...    73   3e-12
UniRef50_Q922A0 Cluster: Enolase; n=7; Amniota|Rep: Enolase - Mu...    72   8e-12
UniRef50_Q9PDT8 Cluster: Enolase; n=217; cellular organisms|Rep:...    71   2e-11
UniRef50_A6R3H2 Cluster: Predicted protein; n=2; Ajellomyces cap...    69   1e-10
UniRef50_A6C3L3 Cluster: Enolase; n=1; Planctomyces maris DSM 87...    64   2e-09
UniRef50_Q74J64 Cluster: Enolase 2; n=10; Bacteria|Rep: Enolase ...    63   5e-09
UniRef50_Q8SUA4 Cluster: Enolase; n=1; Encephalitozoon cuniculi|...    60   3e-08
UniRef50_Q9Y927 Cluster: Enolase; n=8; Archaea|Rep: Enolase - Ae...    58   1e-07
UniRef50_Q0PQU6 Cluster: Enolase 2-phosphoglycerate dehydratase;...    57   3e-07
UniRef50_Q7R0Y1 Cluster: GLP_25_44193_44645; n=1; Giardia lambli...    57   3e-07
UniRef50_Q73V81 Cluster: Enolase; n=3; Bacteria|Rep: Enolase - M...    54   3e-06
UniRef50_UPI00005563F2 Cluster: Phosphopyruvate hydratase; n=1; ...    52   9e-06
UniRef50_A5B6U7 Cluster: Enolase; n=1; Vitis vinifera|Rep: Enola...    52   1e-05
UniRef50_A3BY93 Cluster: Enolase; n=90; root|Rep: Enolase - Oryz...    51   2e-05
UniRef50_P29201 Cluster: Enolase; n=15; Euryarchaeota|Rep: Enola...    51   2e-05
UniRef50_A2E9S4 Cluster: Enolase; n=38; Parabasalidea|Rep: Enola...    51   2e-05
UniRef50_A0RY13 Cluster: Enolase; n=2; Thermoprotei|Rep: Enolase...    50   5e-05
UniRef50_A2TVP0 Cluster: Putative uncharacterized protein; n=1; ...    47   3e-04
UniRef50_Q979Z9 Cluster: Enolase; n=4; Thermoplasmatales|Rep: En...    46   5e-04
UniRef50_Q97ZJ3 Cluster: Enolase; n=4; Sulfolobaceae|Rep: Enolas...    46   5e-04
UniRef50_A7PY41 Cluster: Chromosome chr15 scaffold_37, whole gen...    44   0.002
UniRef50_A5AK08 Cluster: Putative uncharacterized protein; n=1; ...    44   0.002
UniRef50_A2FQV9 Cluster: Enolase; n=1; Trichomonas vaginalis G3|...    44   0.002
UniRef50_A2XEW0 Cluster: Enolase; n=4; Oryza sativa|Rep: Enolase...    43   0.004
UniRef50_Q9V192 Cluster: Enolase; n=4; Thermococcaceae|Rep: Enol...    43   0.006
UniRef50_UPI0000EB12F9 Cluster: UPI0000EB12F9 related cluster; n...    42   0.013
UniRef50_A1WLU9 Cluster: Phosphopyruvate hydratase precursor; n=...    42   0.013
UniRef50_Q8ZYE7 Cluster: Enolase; n=6; Thermoproteaceae|Rep: Eno...    40   0.052
UniRef50_A7I6T9 Cluster: Enolase; n=1; Candidatus Methanoregula ...    38   0.21 
UniRef50_A3W1T9 Cluster: Poly-beta-hydroxybutyrate polymerase; n...    37   0.28 
UniRef50_UPI0001509D31 Cluster: Enolase, N-terminal domain conta...    36   0.48 
UniRef50_Q4V791 Cluster: N-myc (And STAT) interactor; n=3; Xenop...    36   0.85 
UniRef50_A2UP12 Cluster: Putative uncharacterized protein; n=3; ...    35   1.5  
UniRef50_A5UN61 Cluster: Putative uncharacterized protein; n=1; ...    35   1.5  
UniRef50_A7JUJ6 Cluster: Putative uncharacterized protein; n=2; ...    33   3.4  
UniRef50_A6SC20 Cluster: Putative uncharacterized protein; n=1; ...    33   6.0  

>UniRef50_Q8CF78 Cluster: Enolase; n=4; Murinae|Rep: Enolase - Mus
           musculus (Mouse)
          Length = 321

 Score = 93.9 bits (223), Expect = 2e-18
 Identities = 46/85 (54%), Positives = 59/85 (69%)
 Frame = +3

Query: 255 ALELRDNIKSEYHGKGVLTAIKNINELIAPELTKANLEVTQQREIDELMLKLDGTENKSK 434
           ALELRDN K+ + GKGV  A+++IN+ IAP L    + V +Q +ID+LM+++DGTENKSK
Sbjct: 46  ALELRDNDKTRFMGKGVSQAVEHINKTIAPALVSKKVNVVEQEKIDKLMIEMDGTENKSK 105

Query: 435 LGANAILGVSLXXXXXXXXXXNVPL 509
            GANAILGVSL           VPL
Sbjct: 106 FGANAILGVSLAVCKAGAVEKGVPL 130



 Score = 74.5 bits (175), Expect = 1e-12
 Identities = 37/60 (61%), Positives = 42/60 (70%)
 Frame = +1

Query: 121 MVIKSIKARQIFDSRGNPTVEVDLVTELGLFRAAVPSGASTGVHELLN*EITSRVNIMAR 300
           M I  I AR+IFDSRGNPTVEVDL T  GLFRAAVPSGASTG++E L      +   M +
Sbjct: 1   MSILRIHAREIFDSRGNPTVEVDLYTAKGLFRAAVPSGASTGIYEALELRDNDKTRFMGK 60



 Score = 32.3 bits (70), Expect = 7.9
 Identities = 12/31 (38%), Positives = 20/31 (64%)
 Frame = +2

Query: 464 LSCC*GWCCQEECSAYKHLADLAGNNDIVLP 556
           L+ C     ++    Y+H+ADLAGN +++LP
Sbjct: 116 LAVCKAGAVEKGVPLYRHIADLAGNPEVILP 146


>UniRef50_P13929 Cluster: Beta-enolase; n=32; Fungi/Metazoa
           group|Rep: Beta-enolase - Homo sapiens (Human)
          Length = 434

 Score = 93.1 bits (221), Expect = 4e-18
 Identities = 46/85 (54%), Positives = 55/85 (64%)
 Frame = +3

Query: 255 ALELRDNIKSEYHGKGVLTAIKNINELIAPELTKANLEVTQQREIDELMLKLDGTENKSK 434
           ALELRD  K  Y GKGVL A++NIN  + P L +  L V  Q ++D+ M++LDGTENKSK
Sbjct: 46  ALELRDGDKGRYLGKGVLKAVENINNTLGPALLQKKLSVVDQEKVDKFMIELDGTENKSK 105

Query: 435 LGANAILGVSLXXXXXXXXXXNVPL 509
            GANAILGVSL           VPL
Sbjct: 106 FGANAILGVSLAVCKAGAAEKGVPL 130



 Score = 68.5 bits (160), Expect = 1e-10
 Identities = 33/47 (70%), Positives = 38/47 (80%)
 Frame = +1

Query: 121 MVIKSIKARQIFDSRGNPTVEVDLVTELGLFRAAVPSGASTGVHELL 261
           M ++ I AR+I DSRGNPTVEVDL T  G FRAAVPSGASTG++E L
Sbjct: 1   MAMQKIFAREILDSRGNPTVEVDLHTAKGRFRAAVPSGASTGIYEAL 47



 Score = 33.9 bits (74), Expect = 2.6
 Identities = 13/31 (41%), Positives = 20/31 (64%)
 Frame = +2

Query: 464 LSCC*GWCCQEECSAYKHLADLAGNNDIVLP 556
           L+ C     ++    Y+H+ADLAGN D++LP
Sbjct: 116 LAVCKAGAAEKGVPLYRHIADLAGNPDLILP 146


>UniRef50_UPI000066089D Cluster: Gamma-enolase (EC 4.2.1.11)
           (2-phospho-D-glycerate hydro-lyase) (Neural enolase)
           (Neuron-specific enolase) (NSE) (Enolase 2).; n=20;
           Euteleostomi|Rep: Gamma-enolase (EC 4.2.1.11)
           (2-phospho-D-glycerate hydro-lyase) (Neural enolase)
           (Neuron-specific enolase) (NSE) (Enolase 2). - Takifugu
           rubripes
          Length = 438

 Score = 90.6 bits (215), Expect = 2e-17
 Identities = 43/85 (50%), Positives = 57/85 (67%)
 Frame = +3

Query: 255 ALELRDNIKSEYHGKGVLTAIKNINELIAPELTKANLEVTQQREIDELMLKLDGTENKSK 434
           ALELRD  KS Y GKGVL A+ +IN+ + P L  + + V +Q ++D +M+++DGTENKSK
Sbjct: 51  ALELRDGDKSRYKGKGVLKAVGHINDTLGPALIASEICVVEQEQLDNMMIQMDGTENKSK 110

Query: 435 LGANAILGVSLXXXXXXXXXXNVPL 509
            GANAILGVSL           +PL
Sbjct: 111 FGANAILGVSLAICKAGAAEKEIPL 135



 Score = 72.1 bits (169), Expect = 8e-12
 Identities = 35/48 (72%), Positives = 40/48 (83%)
 Frame = +1

Query: 118 KMVIKSIKARQIFDSRGNPTVEVDLVTELGLFRAAVPSGASTGVHELL 261
           +M I  I AR+I DSRGNPTVEVDL TE GLFRA+VPSGASTG++E L
Sbjct: 5   RMSILRIVAREILDSRGNPTVEVDLHTEKGLFRASVPSGASTGIYEAL 52



 Score = 36.3 bits (80), Expect = 0.48
 Identities = 14/31 (45%), Positives = 21/31 (67%)
 Frame = +2

Query: 464 LSCC*GWCCQEECSAYKHLADLAGNNDIVLP 556
           L+ C     ++E   Y+H+ADLAGN ++VLP
Sbjct: 121 LAICKAGAAEKEIPLYRHIADLAGNTELVLP 151


>UniRef50_A7PGJ9 Cluster: Chromosome chr17 scaffold_16, whole genome
           shotgun sequence; n=1; Vitis vinifera|Rep: Chromosome
           chr17 scaffold_16, whole genome shotgun sequence - Vitis
           vinifera (Grape)
          Length = 458

 Score = 90.6 bits (215), Expect = 2e-17
 Identities = 48/85 (56%), Positives = 56/85 (65%)
 Frame = +3

Query: 255 ALELRDNIKSEYHGKGVLTAIKNINELIAPELTKANLEVTQQREIDELMLKLDGTENKSK 434
           ALELRD  K+ Y GKGVL A+ NIN L+AP+L    L+V  Q E+D +ML+ DGT NKSK
Sbjct: 88  ALELRDGDKNVYGGKGVLNAVSNINHLLAPKLV--GLDVRNQAEVDAIMLEFDGTPNKSK 145

Query: 435 LGANAILGVSLXXXXXXXXXXNVPL 509
           LGANA LGVSL           VPL
Sbjct: 146 LGANATLGVSLSVCRAGAGAKGVPL 170



 Score = 72.9 bits (171), Expect = 5e-12
 Identities = 34/46 (73%), Positives = 42/46 (91%)
 Frame = +1

Query: 124 VIKSIKARQIFDSRGNPTVEVDLVTELGLFRAAVPSGASTGVHELL 261
           ++KS+KARQI DSRGNPTVEVDLVT+  L+R+AVPSGASTG++E L
Sbjct: 45  LVKSVKARQIIDSRGNPTVEVDLVTD-NLYRSAVPSGASTGIYEAL 89


>UniRef50_Q0HL72 Cluster: Enolase; n=126; Bacteria|Rep: Enolase -
           Shewanella sp. (strain MR-4)
          Length = 431

 Score = 82.2 bits (194), Expect = 7e-15
 Identities = 45/85 (52%), Positives = 53/85 (62%)
 Frame = +3

Query: 255 ALELRDNIKSEYHGKGVLTAIKNINELIAPELTKANLEVTQQREIDELMLKLDGTENKSK 434
           ALELRD  KS Y GKGVLTA+ N+N  I   L     + T Q E+D++M+ LDGTENK K
Sbjct: 48  ALELRDGDKSRYLGKGVLTAVANVNGPIRAALI--GKDATAQAELDQIMIDLDGTENKDK 105

Query: 435 LGANAILGVSLXXXXXXXXXXNVPL 509
           LGANAIL VSL           +PL
Sbjct: 106 LGANAILAVSLAAAKAAAAFKGMPL 130



 Score = 46.4 bits (105), Expect = 5e-04
 Identities = 25/46 (54%), Positives = 30/46 (65%), Gaps = 1/46 (2%)
 Frame = +1

Query: 127 IKSIKARQIFDSRGNPTVEVDLVTELGLF-RAAVPSGASTGVHELL 261
           I ++  R+I DSRGNPTVE ++  E G    AA PSGASTG  E L
Sbjct: 4   IINVIGREIMDSRGNPTVEAEVHLEGGFIGMAAAPSGASTGSREAL 49


>UniRef50_Q05524 Cluster: Alpha-enolase, lung specific; n=613;
           root|Rep: Alpha-enolase, lung specific - Homo sapiens
           (Human)
          Length = 458

 Score = 82.2 bits (194), Expect = 7e-15
 Identities = 44/72 (61%), Positives = 54/72 (75%), Gaps = 2/72 (2%)
 Frame = +3

Query: 258 LELRDNIKSEYHG-KGVLTAIKNI-NELIAPELTKANLEVTQQREIDELMLKLDGTENKS 431
           LELRDN K+ Y G KGV  A+++I N+ IAP L   N+ V +Q +ID LML +DG+ENKS
Sbjct: 51  LELRDNDKTRYMGGKGVSKAVEHIINKTIAPALISKNVNVVEQDKIDNLMLDMDGSENKS 110

Query: 432 KLGANAILGVSL 467
           K GANAILGVSL
Sbjct: 111 KFGANAILGVSL 122



 Score = 64.5 bits (150), Expect = 2e-09
 Identities = 34/48 (70%), Positives = 39/48 (81%), Gaps = 2/48 (4%)
 Frame = +1

Query: 124 VIKSIKARQIFDSRGNPTVEVDLVTEL-GLF-RAAVPSGASTGVHELL 261
           ++K I AR IF+SRGNPTVEVDL T   GLF RAAVPSGASTG++E L
Sbjct: 3   ILKIIHARDIFESRGNPTVEVDLYTNKGGLFGRAAVPSGASTGIYEAL 50


>UniRef50_Q27727 Cluster: Enolase; n=72; cellular organisms|Rep:
           Enolase - Plasmodium falciparum
          Length = 446

 Score = 81.0 bits (191), Expect = 2e-14
 Identities = 47/77 (61%), Positives = 58/77 (75%), Gaps = 6/77 (7%)
 Frame = +3

Query: 255 ALELRDNIKSEYHGKGVLTAIKNINELIAPELTKANLEVTQQREIDELML-KLDGTEN-- 425
           ALELRDN KS Y GKGV  AIKNINE+IAP+L   N   T+Q++ID LM+ +LDG++N  
Sbjct: 48  ALELRDNDKSRYLGKGVQKAIKNINEIIAPKLIGMN--CTEQKKIDNLMVEELDGSKNEW 105

Query: 426 ---KSKLGANAILGVSL 467
              KSKLGANAIL +S+
Sbjct: 106 GWSKSKLGANAILAISM 122



 Score = 74.1 bits (174), Expect = 2e-12
 Identities = 35/46 (76%), Positives = 39/46 (84%)
 Frame = +1

Query: 124 VIKSIKARQIFDSRGNPTVEVDLVTELGLFRAAVPSGASTGVHELL 261
           VI  I AR+I DSRGNPTVEVDL T LG+FRAAVPSGASTG++E L
Sbjct: 4   VITRINAREILDSRGNPTVEVDLETNLGIFRAAVPSGASTGIYEAL 49


>UniRef50_Q7NAY0 Cluster: Enolase; n=71; cellular organisms|Rep:
           Enolase - Mycoplasma gallisepticum
          Length = 475

 Score = 81.0 bits (191), Expect = 2e-14
 Identities = 42/85 (49%), Positives = 57/85 (67%)
 Frame = +3

Query: 255 ALELRDNIKSEYHGKGVLTAIKNINELIAPELTKANLEVTQQREIDELMLKLDGTENKSK 434
           ALELRD   ++YHGKGV  A+ NIN+ I P++    ++ T Q +IDE M++LDGT+ K+K
Sbjct: 59  ALELRDG-GTKYHGKGVTKAVNNINKKIGPKIL--GVDATLQTQIDEFMIELDGTKTKAK 115

Query: 435 LGANAILGVSLXXXXXXXXXXNVPL 509
           LGANAIL VS+          N+PL
Sbjct: 116 LGANAILAVSMAVCRAAAKSLNLPL 140



 Score = 42.7 bits (96), Expect = 0.006
 Identities = 28/55 (50%), Positives = 33/55 (60%), Gaps = 1/55 (1%)
 Frame = +1

Query: 100 STSSVLKMVIKSIKARQIFDSRGNPTVEVDLVTELG-LFRAAVPSGASTGVHELL 261
           STS   K+ IKS+ A Q FDSRG PTV  ++V   G    + V SGASTG  E L
Sbjct: 6   STSKNNKLEIKSVFAYQAFDSRGFPTVACEVVLNDGSKGLSMVSSGASTGEKEAL 60


>UniRef50_Q8KG25 Cluster: Enolase 2; n=22; Bacteria|Rep: Enolase 2 -
           Chlorobium tepidum
          Length = 437

 Score = 80.6 bits (190), Expect = 2e-14
 Identities = 42/71 (59%), Positives = 53/71 (74%)
 Frame = +3

Query: 255 ALELRDNIKSEYHGKGVLTAIKNINELIAPELTKANLEVTQQREIDELMLKLDGTENKSK 434
           A+ELRD  KS + GKGVL A++N+N LI   L    ++VT+Q  ID  +++LDGT NKSK
Sbjct: 47  AVELRDKDKSVFLGKGVLKAVENVNTLINDALL--GMDVTEQEAIDAKLIELDGTPNKSK 104

Query: 435 LGANAILGVSL 467
           LGANAILGVSL
Sbjct: 105 LGANAILGVSL 115



 Score = 67.7 bits (158), Expect = 2e-10
 Identities = 34/44 (77%), Positives = 35/44 (79%)
 Frame = +1

Query: 124 VIKSIKARQIFDSRGNPTVEVDLVTELGLFRAAVPSGASTGVHE 255
           VI  I ARQI DSRGNPTVEVD+ TE    RAAVPSGASTGVHE
Sbjct: 3   VITRIHARQIMDSRGNPTVEVDVHTESSFGRAAVPSGASTGVHE 46


>UniRef50_A4H7T5 Cluster: Enolase; n=2; cellular organisms|Rep:
           Enolase - Leishmania braziliensis
          Length = 499

 Score = 79.8 bits (188), Expect = 4e-14
 Identities = 42/85 (49%), Positives = 54/85 (63%)
 Frame = +3

Query: 255 ALELRDNIKSEYHGKGVLTAIKNINELIAPELTKANLEVTQQREIDELMLKLDGTENKSK 434
           A ELRD  K+ Y G G   A++N+NE++AP L     EV+ Q  +D+LM +LDGT+NKSK
Sbjct: 197 ACELRDGDKTAYCGAGCTKAVRNVNEILAPALL--GKEVSDQTGLDKLMCELDGTKNKSK 254

Query: 435 LGANAILGVSLXXXXXXXXXXNVPL 509
           LGANAILG S+           VPL
Sbjct: 255 LGANAILGCSMAISKAAAAAAGVPL 279



 Score = 72.1 bits (169), Expect = 8e-12
 Identities = 31/45 (68%), Positives = 40/45 (88%)
 Frame = +1

Query: 121 MVIKSIKARQIFDSRGNPTVEVDLVTELGLFRAAVPSGASTGVHE 255
           M I+ + AR++ DSRGNPTVEV++ TE+G+FR+AVPSGASTGVHE
Sbjct: 152 MPIQKVYAREVLDSRGNPTVEVEVTTEVGVFRSAVPSGASTGVHE 196


>UniRef50_Q6F0Z7 Cluster: Enolase; n=349; cellular organisms|Rep:
           Enolase - Mesoplasma florum (Acholeplasma florum)
          Length = 453

 Score = 79.4 bits (187), Expect = 5e-14
 Identities = 43/85 (50%), Positives = 53/85 (62%)
 Frame = +3

Query: 255 ALELRDNIKSEYHGKGVLTAIKNINELIAPELTKANLEVTQQREIDELMLKLDGTENKSK 434
           ALELRD  K+ Y+GKGVL A+ N+N+ IAP L     +V  Q  +D +M+KLDGTE K K
Sbjct: 48  ALELRDGDKARYNGKGVLKAVANVNDKIAPAL--IGHDVQDQLGLDRVMIKLDGTEFKKK 105

Query: 435 LGANAILGVSLXXXXXXXXXXNVPL 509
           LGAN +L VSL           VPL
Sbjct: 106 LGANGMLAVSLAAAHAAASELEVPL 130



 Score = 51.2 bits (117), Expect = 2e-05
 Identities = 27/46 (58%), Positives = 33/46 (71%), Gaps = 1/46 (2%)
 Frame = +1

Query: 127 IKSIKARQIFDSRGNPTVEVDLVTELGLFR-AAVPSGASTGVHELL 261
           I+ I AR++ DSRG PTVEV+L TE G +  A  PSGASTG +E L
Sbjct: 4   IEKIIAREVLDSRGTPTVEVELWTEFGGYGIAKAPSGASTGENEAL 49


>UniRef50_A5DEC9 Cluster: Enolase; n=1; Pichia guilliermondii|Rep:
           Enolase - Pichia guilliermondii (Yeast) (Candida
           guilliermondii)
          Length = 186

 Score = 78.2 bits (184), Expect = 1e-13
 Identities = 35/71 (49%), Positives = 51/71 (71%)
 Frame = +3

Query: 255 ALELRDNIKSEYHGKGVLTAIKNINELIAPELTKANLEVTQQREIDELMLKLDGTENKSK 434
           A+ELRD  KS++ GKGV  A+ N+N +IAP + K ++++  Q+ +D+ +  L GT+NKS 
Sbjct: 46  AIELRDGDKSKWLGKGVTKAVHNVNTVIAPAIIKEDMDIKNQQPVDDFLNSLYGTDNKSN 105

Query: 435 LGANAILGVSL 467
           LG N ILGVSL
Sbjct: 106 LGTNTILGVSL 116



 Score = 64.9 bits (151), Expect = 1e-09
 Identities = 30/47 (63%), Positives = 35/47 (74%)
 Frame = +1

Query: 121 MVIKSIKARQIFDSRGNPTVEVDLVTELGLFRAAVPSGASTGVHELL 261
           M IK I  +  +DSRGNPTVEV L+T  GLFR+ VPSGASTG HE +
Sbjct: 1   MTIKKIHDQYAYDSRGNPTVEVKLITNKGLFRSIVPSGASTGSHEAI 47


>UniRef50_Q7VQH3 Cluster: Enolase; n=9; Bacteria|Rep: Enolase -
           Blochmannia floridanus
          Length = 447

 Score = 75.8 bits (178), Expect = 6e-13
 Identities = 41/85 (48%), Positives = 53/85 (62%)
 Frame = +3

Query: 255 ALELRDNIKSEYHGKGVLTAIKNINELIAPELTKANLEVTQQREIDELMLKLDGTENKSK 434
           ALELRDN  + + GKGV  ++  IN  I   L   N++VT+Q  IDE+M+ LDGT NKS+
Sbjct: 48  ALELRDNDHARFFGKGVKKSVNIINSTIRVSLL--NIDVTKQSVIDEIMINLDGTNNKSQ 105

Query: 435 LGANAILGVSLXXXXXXXXXXNVPL 509
           LGAN+IL VSL           +PL
Sbjct: 106 LGANSILSVSLAIAKAAASFMGMPL 130



 Score = 46.8 bits (106), Expect = 3e-04
 Identities = 25/46 (54%), Positives = 33/46 (71%), Gaps = 1/46 (2%)
 Frame = +1

Query: 127 IKSIKARQIFDSRGNPTVEVDLVTELGLF-RAAVPSGASTGVHELL 261
           I +I +R+I DSRGNPTVE ++ T+ G F  A+VPSG+S G  E L
Sbjct: 4   IVNIISREIVDSRGNPTVESEVHTKSGFFGLASVPSGSSLGSQEAL 49


>UniRef50_A2A756 Cluster: Enolase 1, alpha non-neuron; n=3;
           Eutheria|Rep: Enolase 1, alpha non-neuron - Mus musculus
           (Mouse)
          Length = 67

 Score = 74.5 bits (175), Expect = 1e-12
 Identities = 37/60 (61%), Positives = 42/60 (70%)
 Frame = +1

Query: 121 MVIKSIKARQIFDSRGNPTVEVDLVTELGLFRAAVPSGASTGVHELLN*EITSRVNIMAR 300
           M I  I AR+IFDSRGNPTVEVDL T  GLFRAAVPSGASTG++E L      +   M +
Sbjct: 1   MSILRIHAREIFDSRGNPTVEVDLYTAKGLFRAAVPSGASTGIYEALELRDNDKTRFMGK 60


>UniRef50_Q9PQV9 Cluster: Enolase; n=1; Ureaplasma parvum|Rep:
           Enolase - Ureaplasma parvum (Ureaplasma urealyticum
           biotype 1)
          Length = 440

 Score = 73.3 bits (172), Expect = 3e-12
 Identities = 39/71 (54%), Positives = 48/71 (67%)
 Frame = +3

Query: 255 ALELRDNIKSEYHGKGVLTAIKNINELIAPELTKANLEVTQQREIDELMLKLDGTENKSK 434
           ALELRD   + +  K V  AI+NIN +I P L   N  V    E+D L++ LDGTENKSK
Sbjct: 47  ALELRDGDVNYFFNKSVKLAIQNINNIIRPHLINKN--VLNFFELDNLLINLDGTENKSK 104

Query: 435 LGANAILGVSL 467
           LGANA+LGVS+
Sbjct: 105 LGANALLGVSI 115



 Score = 40.7 bits (91), Expect = 0.022
 Identities = 26/48 (54%), Positives = 28/48 (58%), Gaps = 1/48 (2%)
 Frame = +1

Query: 121 MVIKSIKARQIFDSRGNPTVEVDLVTELG-LFRAAVPSGASTGVHELL 261
           M I ++ A QI DSRG PTV V L  E      A VPSGASTG  E L
Sbjct: 1   MKIINLLAYQILDSRGQPTVAVKLFLENDQSVIAMVPSGASTGAKEAL 48


>UniRef50_Q922A0 Cluster: Enolase; n=7; Amniota|Rep: Enolase - Mus
           musculus (Mouse)
          Length = 338

 Score = 72.1 bits (169), Expect = 8e-12
 Identities = 35/47 (74%), Positives = 39/47 (82%)
 Frame = +1

Query: 121 MVIKSIKARQIFDSRGNPTVEVDLVTELGLFRAAVPSGASTGVHELL 261
           M I+ I AR+I DSRGNPTVEVDL T  GLFRAAVPSGASTG++E L
Sbjct: 24  MSIEKIWAREILDSRGNPTVEVDLYTAKGLFRAAVPSGASTGIYEAL 70



 Score = 71.7 bits (168), Expect = 1e-11
 Identities = 34/59 (57%), Positives = 42/59 (71%)
 Frame = +3

Query: 255 ALELRDNIKSEYHGKGVLTAIKNINELIAPELTKANLEVTQQREIDELMLKLDGTENKS 431
           ALELRD  K  Y GKGVL A+ +IN  IAP L  + + V +Q ++D LML+LDGTENKS
Sbjct: 69  ALELRDGDKQRYLGKGVLKAVDHINSRIAPALISSGISVVEQEKLDNLMLELDGTENKS 127


>UniRef50_Q9PDT8 Cluster: Enolase; n=217; cellular organisms|Rep:
           Enolase - Xylella fastidiosa
          Length = 430

 Score = 70.9 bits (166), Expect = 2e-11
 Identities = 36/71 (50%), Positives = 47/71 (66%)
 Frame = +3

Query: 255 ALELRDNIKSEYHGKGVLTAIKNINELIAPELTKANLEVTQQREIDELMLKLDGTENKSK 434
           A+ELRD  K+ Y GKGV  A+ N+N +IA  L     +   Q  +D  ++ LDGTENK +
Sbjct: 48  AVELRDGDKTRYLGKGVRAAVDNVNGVIAAALV--GFDGADQTGLDHRLINLDGTENKGR 105

Query: 435 LGANAILGVSL 467
           LGANA+LGVSL
Sbjct: 106 LGANALLGVSL 116



 Score = 50.0 bits (114), Expect = 4e-05
 Identities = 26/44 (59%), Positives = 31/44 (70%), Gaps = 1/44 (2%)
 Frame = +1

Query: 127 IKSIKARQIFDSRGNPTVEVDLVTELGLF-RAAVPSGASTGVHE 255
           I  I AR+I DSRGNPT+E ++  E  +  RAAVPSGASTG  E
Sbjct: 4   IAKIYAREILDSRGNPTLEAEVTLENAVCGRAAVPSGASTGTKE 47


>UniRef50_A6R3H2 Cluster: Predicted protein; n=2; Ajellomyces
           capsulatus NAm1|Rep: Predicted protein - Ajellomyces
           capsulatus NAm1
          Length = 193

 Score = 68.5 bits (160), Expect = 1e-10
 Identities = 32/43 (74%), Positives = 35/43 (81%)
 Frame = +1

Query: 118 KMVIKSIKARQIFDSRGNPTVEVDLVTELGLFRAAVPSGASTG 246
           KM I  I AR ++DSRGNPTVEVD+VTE GL RA VPSGASTG
Sbjct: 149 KMAITKIHARSVYDSRGNPTVEVDVVTETGLHRAIVPSGASTG 191


>UniRef50_A6C3L3 Cluster: Enolase; n=1; Planctomyces maris DSM
           8797|Rep: Enolase - Planctomyces maris DSM 8797
          Length = 456

 Score = 64.1 bits (149), Expect = 2e-09
 Identities = 34/71 (47%), Positives = 45/71 (63%)
 Frame = +3

Query: 255 ALELRDNIKSEYHGKGVLTAIKNINELIAPELTKANLEVTQQREIDELMLKLDGTENKSK 434
           A+ELRD     + G GV  A++N+   IA  L     + + Q  ID ++ +LDGTENKS+
Sbjct: 48  AVELRDQDADRFDGLGVSQAVENVRREIAAALI--GQDASNQSGIDAILCELDGTENKSR 105

Query: 435 LGANAILGVSL 467
           LGANAILG SL
Sbjct: 106 LGANAILGASL 116



 Score = 47.6 bits (108), Expect = 2e-04
 Identities = 25/44 (56%), Positives = 31/44 (70%), Gaps = 1/44 (2%)
 Frame = +1

Query: 127 IKSIKARQIFDSRGNPTVEVDL-VTELGLFRAAVPSGASTGVHE 255
           I+ + AR++FDSRGNPTVEV++        RA VPSGASTG  E
Sbjct: 4   IEYVHARELFDSRGNPTVEVEICCAGSRCGRAIVPSGASTGKFE 47


>UniRef50_Q74J64 Cluster: Enolase 2; n=10; Bacteria|Rep: Enolase 2 -
           Lactobacillus johnsonii
          Length = 428

 Score = 62.9 bits (146), Expect = 5e-09
 Identities = 37/71 (52%), Positives = 44/71 (61%)
 Frame = +3

Query: 255 ALELRDNIKSEYHGKGVLTAIKNINELIAPELTKANLEVTQQREIDELMLKLDGTENKSK 434
           A+ELRD   +   GKGV  A+ N+N  I   L    L    Q EID  M+KLDGT NK+K
Sbjct: 49  AVELRDG-GNRLQGKGVTQAVTNVNGPINDALK--GLSPYNQAEIDRTMIKLDGTLNKAK 105

Query: 435 LGANAILGVSL 467
           LGANAILG S+
Sbjct: 106 LGANAILGTSM 116



 Score = 48.0 bits (109), Expect = 1e-04
 Identities = 24/48 (50%), Positives = 33/48 (68%), Gaps = 1/48 (2%)
 Frame = +1

Query: 115 LKMVIKSIKARQIFDSRGNPTVEVDLVTELG-LFRAAVPSGASTGVHE 255
           + + ++ ++A +IFDSRGNPTVEV      G + +A VPSGASTG  E
Sbjct: 1   MTVYVEKVRALEIFDSRGNPTVEVHAYLSDGTVAKAEVPSGASTGEKE 48


>UniRef50_Q8SUA4 Cluster: Enolase; n=1; Encephalitozoon
           cuniculi|Rep: Enolase - Encephalitozoon cuniculi
          Length = 412

 Score = 60.5 bits (140), Expect = 3e-08
 Identities = 30/70 (42%), Positives = 45/70 (64%)
 Frame = +3

Query: 255 ALELRDNIKSEYHGKGVLTAIKNINELIAPELTKANLEVTQQREIDELMLKLDGTENKSK 434
           A+EL D  +  Y+G+GV T I NIN+L+  ++ +    V  Q+ ID  +L LDGT+NKS+
Sbjct: 50  AVELLDGGEF-YNGRGVETVINNINQLVVKKMCELECNVGDQQAIDNYLLGLDGTKNKSR 108

Query: 435 LGANAILGVS 464
           +G N I  +S
Sbjct: 109 IGGNGITALS 118



 Score = 52.0 bits (119), Expect = 9e-06
 Identities = 25/47 (53%), Positives = 31/47 (65%)
 Frame = +1

Query: 115 LKMVIKSIKARQIFDSRGNPTVEVDLVTELGLFRAAVPSGASTGVHE 255
           +K  +  IK R I  SRG PTVEVDL+T  G+ R++ PSGAS G  E
Sbjct: 3   VKDALLDIKPRMILTSRGRPTVEVDLITSRGVHRSSCPSGASKGSKE 49


>UniRef50_Q9Y927 Cluster: Enolase; n=8; Archaea|Rep: Enolase -
           Aeropyrum pernix
          Length = 432

 Score = 58.4 bits (135), Expect = 1e-07
 Identities = 29/71 (40%), Positives = 46/71 (64%)
 Frame = +3

Query: 255 ALELRDNIKSEYHGKGVLTAIKNINELIAPELTKANLEVTQQREIDELMLKLDGTENKSK 434
           A+ELRD    ++ GKGV  A+  +N ++AP L    ++  +Q +ID L+++LDGT NKS+
Sbjct: 52  AVELRDG-GGKWRGKGVSRAVSLLNTVVAPRLE--GVDARRQAQIDRLLIELDGTPNKSR 108

Query: 435 LGANAILGVSL 467
           LG N    +S+
Sbjct: 109 LGGNTTTALSI 119


>UniRef50_Q0PQU6 Cluster: Enolase 2-phosphoglycerate dehydratase;
           n=1; Endoriftia persephone 'Hot96_1+Hot96_2'|Rep:
           Enolase 2-phosphoglycerate dehydratase - Endoriftia
           persephone 'Hot96_1+Hot96_2'
          Length = 273

 Score = 56.8 bits (131), Expect = 3e-07
 Identities = 30/55 (54%), Positives = 37/55 (67%)
 Frame = +3

Query: 303 VLTAIKNINELIAPELTKANLEVTQQREIDELMLKLDGTENKSKLGANAILGVSL 467
           VL A+ N+N  +   L     EVT Q  +D  ML LDGT+NKSKLGANA+LGVS+
Sbjct: 1   VLNAVGNVNGPLRDALI--GQEVTDQTALDNTMLALDGTDNKSKLGANALLGVSM 53


>UniRef50_Q7R0Y1 Cluster: GLP_25_44193_44645; n=1; Giardia lamblia
           ATCC 50803|Rep: GLP_25_44193_44645 - Giardia lamblia
           ATCC 50803
          Length = 150

 Score = 56.8 bits (131), Expect = 3e-07
 Identities = 30/45 (66%), Positives = 33/45 (73%)
 Frame = -2

Query: 260 KSS*TPVDAPEGTAARNKPSSVTRSTSTVGLPRESKI*RALIDFI 126
           ++S  PVDAPEG AARN PS V  STS VG+PRES I RALI  I
Sbjct: 82  RASCIPVDAPEGHAARNTPSWVVSSTSVVGVPRESMIMRALIALI 126


>UniRef50_Q73V81 Cluster: Enolase; n=3; Bacteria|Rep: Enolase -
           Mycobacterium paratuberculosis
          Length = 427

 Score = 53.6 bits (123), Expect = 3e-06
 Identities = 28/71 (39%), Positives = 41/71 (57%)
 Frame = +3

Query: 255 ALELRDNIKSEYHGKGVLTAIKNINELIAPELTKANLEVTQQREIDELMLKLDGTENKSK 434
           A  LRD   + Y G+ V  A+  + + IAP LT A L+    R +D +M++LD T +K +
Sbjct: 48  AFVLRDGDPTRYRGRSVHRAVAAVRDEIAPALTGAELD--DPRSLDRVMIELDDTPDKHR 105

Query: 435 LGANAILGVSL 467
           LG NAI   S+
Sbjct: 106 LGGNAIYSTSI 116



 Score = 44.0 bits (99), Expect = 0.002
 Identities = 20/44 (45%), Positives = 28/44 (63%), Gaps = 1/44 (2%)
 Frame = +1

Query: 127 IKSIKARQIFDSRGNPTVEVDLVTELG-LFRAAVPSGASTGVHE 255
           I S+ ARQ+ D +  P VEV++ T+ G + R A P+G S G HE
Sbjct: 4   IASVVARQLLDCKARPLVEVEITTDTGHVGRGAAPTGTSVGAHE 47


>UniRef50_UPI00005563F2 Cluster: Phosphopyruvate hydratase; n=1;
           Paracoccus denitrificans PD1222|Rep: Phosphopyruvate
           hydratase - Paracoccus denitrificans PD1222
          Length = 211

 Score = 52.0 bits (119), Expect = 9e-06
 Identities = 28/55 (50%), Positives = 37/55 (67%)
 Frame = +3

Query: 303 VLTAIKNINELIAPELTKANLEVTQQREIDELMLKLDGTENKSKLGANAILGVSL 467
           +L A+  +N  IA  L     + T+Q  ID +M++LDGT NK +LGANAILGVSL
Sbjct: 1   MLEAVAAVNGEIAENLIGE--DATEQVAIDRMMIELDGTPNKGRLGANAILGVSL 53


>UniRef50_A5B6U7 Cluster: Enolase; n=1; Vitis vinifera|Rep: Enolase
           - Vitis vinifera (Grape)
          Length = 527

 Score = 51.6 bits (118), Expect = 1e-05
 Identities = 27/61 (44%), Positives = 38/61 (62%)
 Frame = +3

Query: 255 ALELRDNIKSEYHGKGVLTAIKNINELIAPELTKANLEVTQQREIDELMLKLDGTENKSK 434
           A+ELRD  K  Y G GV  A++N+NE I+  L    ++ T Q +ID++M+ LD TE K  
Sbjct: 65  AVELRDGDKGTYLGNGVTRAVRNVNEKISEAL--IGMDPTLQSQIDQVMIDLDKTEKKVP 122

Query: 435 L 437
           L
Sbjct: 123 L 123


>UniRef50_A3BY93 Cluster: Enolase; n=90; root|Rep: Enolase - Oryza
           sativa subsp. japonica (Rice)
          Length = 516

 Score = 51.2 bits (117), Expect = 2e-05
 Identities = 25/44 (56%), Positives = 29/44 (65%)
 Frame = +3

Query: 378 QREIDELMLKLDGTENKSKLGANAILGVSLXXXXXXXXXXNVPL 509
           Q ++D +ML +DGT NKSKLGANAILGVSL           VPL
Sbjct: 144 QSDVDAIMLDIDGTPNKSKLGANAILGVSLSVCRAGAGAKEVPL 187


>UniRef50_P29201 Cluster: Enolase; n=15; Euryarchaeota|Rep: Enolase
           - Haloarcula marismortui (Halobacterium marismortui)
          Length = 401

 Score = 51.2 bits (117), Expect = 2e-05
 Identities = 25/47 (53%), Positives = 33/47 (70%), Gaps = 1/47 (2%)
 Frame = +1

Query: 124 VIKSIKARQIFDSRGNPTVEVDLVTELGLF-RAAVPSGASTGVHELL 261
           +I  I+ R++ DSRGN TVE D++TE G F R   PSGASTG +E +
Sbjct: 3   LITDIRLRRVLDSRGNATVEADVLTESGGFGRGKAPSGASTGEYEAI 49


>UniRef50_A2E9S4 Cluster: Enolase; n=38; Parabasalidea|Rep: Enolase
           - Trichomonas vaginalis G3
          Length = 493

 Score = 50.8 bits (116), Expect = 2e-05
 Identities = 30/70 (42%), Positives = 39/70 (55%)
 Frame = +3

Query: 255 ALELRDNIKSEYHGKGVLTAIKNINELIAPELTKANLEVTQQREIDELMLKLDGTENKSK 434
           A ELRD   + + GKGV  A+KN+N +I+  +    LE     EID  ++  DGTE K K
Sbjct: 115 AKELRDG-DNRFGGKGVTHAVKNVNTIISKAIAGKLLE--NLAEIDNAIIAADGTELKEK 171

Query: 435 LGANAILGVS 464
           LG NA    S
Sbjct: 172 LGGNATTATS 181



 Score = 45.2 bits (102), Expect = 0.001
 Identities = 26/51 (50%), Positives = 33/51 (64%), Gaps = 5/51 (9%)
 Frame = +1

Query: 118 KMVIKSIKARQIFDSRGNPTVEVD-----LVTELGLFRAAVPSGASTGVHE 255
           K +I  + AR++ DSRGNPTVEVD     L T   + R++ PSGASTG  E
Sbjct: 64  KPIIDHVLAREVLDSRGNPTVEVDVYAKYLNTVEFVARSSSPSGASTGSKE 114


>UniRef50_A0RY13 Cluster: Enolase; n=2; Thermoprotei|Rep: Enolase -
           Cenarchaeum symbiosum
          Length = 412

 Score = 49.6 bits (113), Expect = 5e-05
 Identities = 24/48 (50%), Positives = 36/48 (75%), Gaps = 2/48 (4%)
 Frame = +1

Query: 127 IKSIKARQIFDSRGNPTVEVDLVTELGLF--RAAVPSGASTGVHELLN 264
           I S++ R +++SRG+ TVEVD++++ G F  RA  PSGAS G+HE+ N
Sbjct: 4   ITSVRGRIVYNSRGSRTVEVDVISD-GKFLGRACAPSGASVGIHEVRN 50


>UniRef50_A2TVP0 Cluster: Putative uncharacterized protein; n=1;
           Dokdonia donghaensis MED134|Rep: Putative
           uncharacterized protein - Dokdonia donghaensis MED134
          Length = 132

 Score = 46.8 bits (106), Expect = 3e-04
 Identities = 26/37 (70%), Positives = 28/37 (75%), Gaps = 1/37 (2%)
 Frame = -2

Query: 257 SS*TPVDAPEGTAARN-KPSSVTRSTSTVGLPRESKI 150
           +S +PVDAPEGTAA    PSSV  STSTVGLP ES I
Sbjct: 93  ASCSPVDAPEGTAALPIAPSSVNTSTSTVGLPLESNI 129


>UniRef50_Q979Z9 Cluster: Enolase; n=4; Thermoplasmatales|Rep:
           Enolase - Thermoplasma volcanium
          Length = 401

 Score = 46.4 bits (105), Expect = 5e-04
 Identities = 20/49 (40%), Positives = 33/49 (67%)
 Frame = +1

Query: 115 LKMVIKSIKARQIFDSRGNPTVEVDLVTELGLFRAAVPSGASTGVHELL 261
           +++ I+ ++ R++ DSRGN TVE D+    G  R + P+GASTG  E++
Sbjct: 1   MELPIEDVRVRKVLDSRGNFTVEADVYIPGGFGRTSAPAGASTGETEVI 49


>UniRef50_Q97ZJ3 Cluster: Enolase; n=4; Sulfolobaceae|Rep: Enolase -
           Sulfolobus solfataricus
          Length = 419

 Score = 46.4 bits (105), Expect = 5e-04
 Identities = 28/71 (39%), Positives = 42/71 (59%)
 Frame = +3

Query: 255 ALELRDNIKSEYHGKGVLTAIKNINELIAPELTKANLEVTQQREIDELMLKLDGTENKSK 434
           A+E+RD      +G  V  A+  +N +I P L    ++V +Q  ID+L+  +D TENKSK
Sbjct: 51  AVEVRDE-----NGLTVKRAVDIVNYIIDPALH--GIDVREQGIIDKLLKDIDSTENKSK 103

Query: 435 LGANAILGVSL 467
           LG N I+  S+
Sbjct: 104 LGGNTIIATSI 114



 Score = 36.7 bits (81), Expect = 0.37
 Identities = 19/44 (43%), Positives = 26/44 (59%), Gaps = 1/44 (2%)
 Frame = +1

Query: 127 IKSIKARQIFDSRGNPTVEVDLVTELGLFR-AAVPSGASTGVHE 255
           I+ +K  +I DSRGNPT+ V + T  G+      P+GAS G  E
Sbjct: 7   IEKVKGLEIVDSRGNPTIRVFIRTSDGVESFGDAPAGASKGTRE 50


>UniRef50_A7PY41 Cluster: Chromosome chr15 scaffold_37, whole genome
           shotgun sequence; n=1; Vitis vinifera|Rep: Chromosome
           chr15 scaffold_37, whole genome shotgun sequence - Vitis
           vinifera (Grape)
          Length = 253

 Score = 44.4 bits (100), Expect = 0.002
 Identities = 25/48 (52%), Positives = 30/48 (62%), Gaps = 1/48 (2%)
 Frame = +1

Query: 103 TSSVLKMVIKSIKARQIFDSRGNPTVEVDLVTELGL-FRAAVPSGAST 243
           TS      I+ +KARQIFD  G+PTVEVD+    G     A+PSGAST
Sbjct: 27  TSKPSMFTIQFMKARQIFDGLGDPTVEVDIGLSNGAEVSIAMPSGAST 74



 Score = 44.4 bits (100), Expect = 0.002
 Identities = 25/48 (52%), Positives = 30/48 (62%), Gaps = 1/48 (2%)
 Frame = +1

Query: 103 TSSVLKMVIKSIKARQIFDSRGNPTVEVDLVTELGL-FRAAVPSGAST 243
           TS      I+ +KARQIFD  G+PTVEVD+    G     A+PSGAST
Sbjct: 147 TSKPSMFTIQFMKARQIFDGLGDPTVEVDIGLSNGAEVSIAMPSGAST 194


>UniRef50_A5AK08 Cluster: Putative uncharacterized protein; n=1;
           Vitis vinifera|Rep: Putative uncharacterized protein -
           Vitis vinifera (Grape)
          Length = 150

 Score = 44.4 bits (100), Expect = 0.002
 Identities = 25/48 (52%), Positives = 30/48 (62%), Gaps = 1/48 (2%)
 Frame = +1

Query: 103 TSSVLKMVIKSIKARQIFDSRGNPTVEVDLVTELGL-FRAAVPSGAST 243
           TS      I+ +KARQIFD  G+PTVEVD+    G     A+PSGAST
Sbjct: 44  TSKPSMFTIQFMKARQIFDGLGDPTVEVDIGLSNGAEVSIAMPSGAST 91


>UniRef50_A2FQV9 Cluster: Enolase; n=1; Trichomonas vaginalis
           G3|Rep: Enolase - Trichomonas vaginalis G3
          Length = 448

 Score = 44.0 bits (99), Expect = 0.002
 Identities = 23/82 (28%), Positives = 38/82 (46%)
 Frame = +3

Query: 264 LRDNIKSEYHGKGVLTAIKNINELIAPELTKANLEVTQQREIDELMLKLDGTENKSKLGA 443
           L D     Y G+G+  A+  +  +  P L K   +   QRE+D  +++ DGT N+ K G+
Sbjct: 106 LVDTSNPRYGGRGMRQAVSAVTSVYQPVLEKK--QFFNQREVDGWLIQADGTPNRRKSGS 163

Query: 444 NAILGVSLXXXXXXXXXXNVPL 509
           N ++  S            +PL
Sbjct: 164 NTMIATSATIAIASSKIMRIPL 185


>UniRef50_A2XEW0 Cluster: Enolase; n=4; Oryza sativa|Rep: Enolase -
           Oryza sativa subsp. indica (Rice)
          Length = 485

 Score = 43.2 bits (97), Expect = 0.004
 Identities = 23/82 (28%), Positives = 44/82 (53%)
 Frame = +3

Query: 264 LRDNIKSEYHGKGVLTAIKNINELIAPELTKANLEVTQQREIDELMLKLDGTENKSKLGA 443
           +RD  K +   + V  A++ IN+ ++  L    ++  QQ +ID+ ++ LD   +K+++G 
Sbjct: 93  VRDAEKRKLLARAVADAVRVINDKVSEALV--GMDPQQQSQIDQAIMDLDKAHHKAEIGV 150

Query: 444 NAILGVSLXXXXXXXXXXNVPL 509
           N++L VS+           VPL
Sbjct: 151 NSMLAVSIAACKAGAAEKEVPL 172



 Score = 38.3 bits (85), Expect = 0.12
 Identities = 18/33 (54%), Positives = 22/33 (66%)
 Frame = +1

Query: 124 VIKSIKARQIFDSRGNPTVEVDLVTELGLFRAA 222
           VI S++ARQI D RG P VEV L T   + RA+
Sbjct: 46  VITSVRARQILDGRGEPAVEVSLHTNKAVHRAS 78


>UniRef50_Q9V192 Cluster: Enolase; n=4; Thermococcaceae|Rep: Enolase
           - Pyrococcus abyssi
          Length = 342

 Score = 42.7 bits (96), Expect = 0.006
 Identities = 20/52 (38%), Positives = 32/52 (61%)
 Frame = +3

Query: 312 AIKNINELIAPELTKANLEVTQQREIDELMLKLDGTENKSKLGANAILGVSL 467
           A+  ++E+I PEL     +  +Q  ID  + ++DGTE+ S +GAN  L VS+
Sbjct: 52  AVSEVDEIIGPELI--GFDAVEQELIDSYLWEIDGTEDFSHIGANTALAVSI 101


>UniRef50_UPI0000EB12F9 Cluster: UPI0000EB12F9 related cluster; n=1;
           Canis lupus familiaris|Rep: UPI0000EB12F9 UniRef100
           entry - Canis familiaris
          Length = 330

 Score = 41.5 bits (93), Expect = 0.013
 Identities = 33/85 (38%), Positives = 46/85 (54%)
 Frame = +3

Query: 255 ALELRDNIKSEYHGKGVLTAIKNINELIAPELTKANLEVTQQREIDELMLKLDGTENKSK 434
           ALE+ DN K+ Y  KGV  A ++IN+ I   L   NL     R+I++LM+K D T+    
Sbjct: 2   ALEILDNDKTCYVVKGVSKA-EHINKTITSTLISKNL----TRKIEKLMIKTDRTD---- 52

Query: 435 LGANAILGVSLXXXXXXXXXXNVPL 509
             AN++LGVSL           +PL
Sbjct: 53  --ANSLLGVSLAVCKAGAIENGMPL 75


>UniRef50_A1WLU9 Cluster: Phosphopyruvate hydratase precursor; n=2;
           Proteobacteria|Rep: Phosphopyruvate hydratase precursor
           - Verminephrobacter eiseniae (strain EF01-2)
          Length = 443

 Score = 41.5 bits (93), Expect = 0.013
 Identities = 28/85 (32%), Positives = 40/85 (47%)
 Frame = +3

Query: 255 ALELRDNIKSEYHGKGVLTAIKNINELIAPELTKANLEVTQQREIDELMLKLDGTENKSK 434
           A +LRD   +   G  VLTA+  +  +IAP L    + VT Q  ID  + +LD +  +  
Sbjct: 70  ASDLRDG-GTRLGGYDVLTALDRVRSIIAPALI--GMAVTDQAAIDATLDRLDPSPTRQL 126

Query: 435 LGANAILGVSLXXXXXXXXXXNVPL 509
           LG NA +  SL           +PL
Sbjct: 127 LGGNATVATSLAALHSAAAVRQMPL 151



 Score = 41.1 bits (92), Expect = 0.017
 Identities = 25/59 (42%), Positives = 34/59 (57%), Gaps = 1/59 (1%)
 Frame = +1

Query: 82  TLNLRKSTSSVLKMVIKSIKARQIFDSRGNPTVEVDLVTELG-LFRAAVPSGASTGVHE 255
           T     S SS  +  I ++  R+++DSRG PTVEV++ T  G   RA  P+GAS G  E
Sbjct: 12  TTTTATSASSATER-IAALHGRRVWDSRGRPTVEVEITTAGGQRGRAIAPAGASRGSAE 69


>UniRef50_Q8ZYE7 Cluster: Enolase; n=6; Thermoproteaceae|Rep:
           Enolase - Pyrobaculum aerophilum
          Length = 419

 Score = 39.5 bits (88), Expect = 0.052
 Identities = 23/53 (43%), Positives = 31/53 (58%), Gaps = 6/53 (11%)
 Frame = +1

Query: 121 MVIKSIKARQIFDSRGNPTVEVDLVTE------LGLFRAAVPSGASTGVHELL 261
           M I     R++F  RG+ TVEV+L  E      + + RAA P+GAS G HE+L
Sbjct: 1   MQISDAWIRKVFTGRGDVTVEVELTVEDSVTGDVLVTRAAAPAGASRGAHEVL 53



 Score = 35.1 bits (77), Expect = 1.1
 Identities = 21/72 (29%), Positives = 33/72 (45%)
 Frame = +3

Query: 300 GVLTAIKNINELIAPELTKANLEVTQQREIDELMLKLDGTENKSKLGANAILGVSLXXXX 479
           GV  A+    +L+APE+    L+VT+    D  + ++DGT+   K+G    +  S     
Sbjct: 59  GVDAALAAFEKLVAPEIV--GLDVTEPYSTDGKLEEVDGTQRFEKIGGAVAIATSFAAAE 116

Query: 480 XXXXXXNVPLTS 515
                  VPL S
Sbjct: 117 AGAASLGVPLYS 128


>UniRef50_A7I6T9 Cluster: Enolase; n=1; Candidatus Methanoregula
           boonei 6A8|Rep: Enolase - Methanoregula boonei (strain
           6A8)
          Length = 55

 Score = 37.5 bits (83), Expect = 0.21
 Identities = 19/43 (44%), Positives = 28/43 (65%)
 Frame = +1

Query: 127 IKSIKARQIFDSRGNPTVEVDLVTELGLFRAAVPSGASTGVHE 255
           ++SI AR+  DSR NP +E +++      RA  PSGASTG ++
Sbjct: 5   LQSIPAREFPDSRSNPAIEGEIMIR-DTVRAVDPSGASTGKNQ 46


>UniRef50_A3W1T9 Cluster: Poly-beta-hydroxybutyrate polymerase; n=5;
           Rhodobacteraceae|Rep: Poly-beta-hydroxybutyrate
           polymerase - Roseovarius sp. 217
          Length = 656

 Score = 37.1 bits (82), Expect = 0.28
 Identities = 16/53 (30%), Positives = 28/53 (52%)
 Frame = +3

Query: 204 WLVPGSCTLWCIHWCS*ALELRDNIKSEYHGKGVLTAIKNINELIAPELTKAN 362
           WLV    T++CI W +   E  D    +Y  +G++ A++ IN ++  +   AN
Sbjct: 334 WLVAQGYTVFCISWRNPGAEHADLSLEDYRNQGIMQALRVINTIVPGQKVHAN 386


>UniRef50_UPI0001509D31 Cluster: Enolase, N-terminal domain containing
            protein; n=1; Tetrahymena thermophila SB210|Rep: Enolase,
            N-terminal domain containing protein - Tetrahymena
            thermophila SB210
          Length = 1593

 Score = 36.3 bits (80), Expect = 0.48
 Identities = 27/67 (40%), Positives = 34/67 (50%)
 Frame = +3

Query: 264  LRDNIKSEYHGKGVLTAIKNINELIAPELTKANLEVTQQREIDELMLKLDGTENKSKLGA 443
            L DNI     GKGV  A++ I   I P L K +     Q++IDE + +L   E   K G 
Sbjct: 1188 LYDNINEVDSGKGVSNALEFIKSKINPILNKKS--ARDQKQIDEQLTQL--YEANEKKGI 1243

Query: 444  NAILGVS 464
            NAI  VS
Sbjct: 1244 NAIQTVS 1250


>UniRef50_Q4V791 Cluster: N-myc (And STAT) interactor; n=3;
           Xenopus|Rep: N-myc (And STAT) interactor - Xenopus
           tropicalis (Western clawed frog) (Silurana tropicalis)
          Length = 462

 Score = 35.5 bits (78), Expect = 0.85
 Identities = 23/74 (31%), Positives = 39/74 (52%), Gaps = 7/74 (9%)
 Frame = +3

Query: 234 CIHW-----CS*ALELRDNIKSEY-HGKGVLTAIKNI-NELIAPELTKANLEVTQQREID 392
           C HW      + ++E    ++SEY H K    A  N  + LI  ++   + ++  QR+++
Sbjct: 84  CDHWKFNRSLNTSMESHGGLQSEYDHWKEKHDAADNRRSNLIMEKVDATDTKIKTQRQVE 143

Query: 393 ELMLKLDGTENKSK 434
           EL  KLDGT+ + K
Sbjct: 144 ELARKLDGTDEEKK 157


>UniRef50_A2UP12 Cluster: Putative uncharacterized protein; n=3;
           Enterobacteriaceae|Rep: Putative uncharacterized protein
           - Escherichia coli B
          Length = 409

 Score = 34.7 bits (76), Expect = 1.5
 Identities = 22/71 (30%), Positives = 35/71 (49%), Gaps = 4/71 (5%)
 Frame = -2

Query: 455 KDSISTQFGFVLSAIQLKHEFINLSLLGYFKVGFGKFRSNQFIDIFDCGQNSLAMIFTLD 276
           +D +  +FGFV  A+Q+ H+ +N SL+      F  F + +  D      NS    FT +
Sbjct: 337 QDCVRAEFGFVFGAVQVDHDLVNASLI------FSIFANQRLSDRAVYRSNSFGYAFTQE 390

Query: 275 ----VISQFKS 255
                I+QF+S
Sbjct: 391 TGFVAIAQFQS 401


>UniRef50_A5UN61 Cluster: Putative uncharacterized protein; n=1;
           Methanobrevibacter smithii ATCC 35061|Rep: Putative
           uncharacterized protein - Methanobrevibacter smithii
           (strain PS / ATCC 35061 / DSM 861)
          Length = 136

 Score = 34.7 bits (76), Expect = 1.5
 Identities = 20/34 (58%), Positives = 20/34 (58%)
 Frame = -2

Query: 245 PVDAPEGTAARNKPSSVTRSTSTVGLPRESKI*R 144
           PV AP G AA   P  V  STSTVG P  SKI R
Sbjct: 77  PVLAPLGAAALPNPFQVITSTSTVGFPLLSKILR 110


>UniRef50_A7JUJ6 Cluster: Putative uncharacterized protein; n=2;
           Mannheimia haemolytica|Rep: Putative uncharacterized
           protein - Mannheimia haemolytica PHL213
          Length = 601

 Score = 33.5 bits (73), Expect = 3.4
 Identities = 21/56 (37%), Positives = 35/56 (62%), Gaps = 1/56 (1%)
 Frame = +3

Query: 297 KGVLTAIK-NINELIAPELTKANLEVTQQREIDELMLKLDGTENKSKLGANAILGV 461
           KG+ T I+ NIN+++     K  L +TQQ + +E++ K+ G   K  LG N++LG+
Sbjct: 522 KGLGTTIEFNINDILKKIFAKHQLSITQQHK-NEVLEKIKGDLLKMDLG-NSVLGL 575


>UniRef50_A6SC20 Cluster: Putative uncharacterized protein; n=1;
           Botryotinia fuckeliana B05.10|Rep: Putative
           uncharacterized protein - Botryotinia fuckeliana B05.10
          Length = 253

 Score = 32.7 bits (71), Expect = 6.0
 Identities = 20/62 (32%), Positives = 33/62 (53%), Gaps = 1/62 (1%)
 Frame = +3

Query: 255 ALELRDNIKSEYHGKGVLTAIKNINELIAPELTKANLEVTQ-QREIDELMLKLDGTENKS 431
           ALELRD  +S Y   GV  A++ +NE++ P +  A+  + +  R +  L  KL     ++
Sbjct: 156 ALELRDGDESIYQCYGVPKAVQIVNEILGPAIISASSMLAKISRTLTFLRAKLTRQVTRA 215

Query: 432 KL 437
            L
Sbjct: 216 SL 217


  Database: uniref50
    Posted date:  Oct 5, 2007 11:19 AM
  Number of letters in database: 575,637,011
  Number of sequences in database:  1,657,284
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 486,514,830
Number of Sequences: 1657284
Number of extensions: 8163293
Number of successful extensions: 19919
Number of sequences better than 10.0: 50
Number of HSP's better than 10.0 without gapping: 19282
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 19884
length of database: 575,637,011
effective HSP length: 96
effective length of database: 416,537,747
effective search space used: 37071859483
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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