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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= NV060394.seq
         (558 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At1g74030.1 68414.m08573 enolase, putative similar to Swiss-Prot...    98   4e-21
At2g29560.1 68415.m03590 enolase, putative similar to enolase [S...    68   5e-12
At2g36530.1 68415.m04481 enolase identical to SWISS-PROT:P25696 ...    64   5e-11
At1g64050.1 68414.m07255 expressed protein                             29   2.8  
At1g32340.1 68414.m03985 zinc finger (C3HC4-type RING finger) fa...    28   3.7  

>At1g74030.1 68414.m08573 enolase, putative similar to
           Swiss-Prot:P15007 enolase (EC 4.2.1.11)
           (2-phosphoglycerate dehydratase)(2-phospho-D- glycerate
           hydro-lyase) [Drosophila melanogaster]
          Length = 477

 Score = 97.9 bits (233), Expect = 4e-21
 Identities = 53/85 (62%), Positives = 60/85 (70%)
 Frame = +3

Query: 255 ALELRDNIKSEYHGKGVLTAIKNINELIAPELTKANLEVTQQREIDELMLKLDGTENKSK 434
           ALELRD  KS Y GKGVL AIKNINEL+AP+L    ++V  Q ++D LML+LDGT NKSK
Sbjct: 93  ALELRDGDKSVYGGKGVLQAIKNINELVAPKLI--GVDVRNQADVDALMLELDGTPNKSK 150

Query: 435 LGANAILGVSLXXXXXXXXXXNVPL 509
           LGANAILGVSL           VPL
Sbjct: 151 LGANAILGVSLSVCRAGAGAKGVPL 175



 Score = 70.1 bits (164), Expect = 9e-13
 Identities = 32/45 (71%), Positives = 40/45 (88%)
 Frame = +1

Query: 127 IKSIKARQIFDSRGNPTVEVDLVTELGLFRAAVPSGASTGVHELL 261
           +K +KARQI DSRGNPTVEVDL+T+  L+R+AVPSGASTG++E L
Sbjct: 51  VKGVKARQIIDSRGNPTVEVDLITD-DLYRSAVPSGASTGIYEAL 94


>At2g29560.1 68415.m03590 enolase, putative similar to enolase
           [Spinacia oleracea] gi|8919731|emb|CAB96173
          Length = 475

 Score = 67.7 bits (158), Expect = 5e-12
 Identities = 39/85 (45%), Positives = 49/85 (57%)
 Frame = +3

Query: 255 ALELRDNIKSEYHGKGVLTAIKNINELIAPELTKANLEVTQQREIDELMLKLDGTENKSK 434
           A+ELRD  K  Y G  V  A+KNINE I+  L    ++   Q +ID+ M+ LD TE KS+
Sbjct: 90  AIELRDGDKGMYLGNSVAKAVKNINEKISEALI--GMDPKLQGQIDQAMIDLDKTEKKSE 147

Query: 435 LGANAILGVSLXXXXXXXXXXNVPL 509
           LGANAIL VS+           VPL
Sbjct: 148 LGANAILAVSIAACKAGAAEKEVPL 172



 Score = 65.3 bits (152), Expect = 3e-11
 Identities = 35/61 (57%), Positives = 44/61 (72%)
 Frame = +1

Query: 79  ITLNLRKSTSSVLKMVIKSIKARQIFDSRGNPTVEVDLVTELGLFRAAVPSGASTGVHEL 258
           I  +L+K+ SSV    I  +KARQI DSRG PTVEVDL T  G+FRA+VPSG S+G +E 
Sbjct: 35  IANHLKKAVSSV----ITKVKARQILDSRGIPTVEVDLHTNKGVFRASVPSGDSSGTYEA 90

Query: 259 L 261
           +
Sbjct: 91  I 91



 Score = 27.9 bits (59), Expect = 4.9
 Identities = 11/31 (35%), Positives = 19/31 (61%)
 Frame = +2

Query: 464 LSCC*GWCCQEECSAYKHLADLAGNNDIVLP 556
           ++ C     ++E    KHL+DL+G  ++VLP
Sbjct: 158 IAACKAGAAEKEVPLCKHLSDLSGRANMVLP 188


>At2g36530.1 68415.m04481 enolase identical to SWISS-PROT:P25696
           enolase (EC 4.2.1.11) (2-phosphoglycerate
           dehydratase)(2-phospho-D- glycerate hydro-lyase)
           [Arabidopsis thaliana]
          Length = 444

 Score = 64.5 bits (150), Expect = 5e-11
 Identities = 43/91 (47%), Positives = 52/91 (57%), Gaps = 6/91 (6%)
 Frame = +3

Query: 255 ALELRDNIKSEYHGKGVLTAIKNINELIAPELTKANLEVTQQREIDELML-KLDGTEN-- 425
           ALELRD   S+Y GKGV  A+ N+N +I P L     + TQQ  ID  M+ +LDGT+N  
Sbjct: 48  ALELRDG-GSDYLGKGVSKAVGNVNNIIGPAL--IGKDPTQQTAIDNFMVHELDGTQNEW 104

Query: 426 ---KSKLGANAILGVSLXXXXXXXXXXNVPL 509
              K KLGANAIL VSL           +PL
Sbjct: 105 GWCKQKLGANAILAVSLAVCKAGAVVSGIPL 135



 Score = 63.7 bits (148), Expect = 8e-11
 Identities = 32/46 (69%), Positives = 37/46 (80%), Gaps = 1/46 (2%)
 Frame = +1

Query: 127 IKSIKARQIFDSRGNPTVEVDLVTELGL-FRAAVPSGASTGVHELL 261
           I  +KARQIFDSRGNPTVEVD+ T  G+   AAVPSGASTG++E L
Sbjct: 4   ITVVKARQIFDSRGNPTVEVDIHTSNGIKVTAAVPSGASTGIYEAL 49



 Score = 29.5 bits (63), Expect = 1.6
 Identities = 12/16 (75%), Positives = 14/16 (87%)
 Frame = +2

Query: 509 YKHLADLAGNNDIVLP 556
           YKH+A+LAGN  IVLP
Sbjct: 136 YKHIANLAGNPKIVLP 151


>At1g64050.1 68414.m07255 expressed protein
          Length = 668

 Score = 28.7 bits (61), Expect = 2.8
 Identities = 13/44 (29%), Positives = 26/44 (59%)
 Frame = -2

Query: 281 LDVISQFKSS*TPVDAPEGTAARNKPSSVTRSTSTVGLPRESKI 150
           +D  S+ ++  +P+ +PE   +  K   ++RS+S  G PR +K+
Sbjct: 96  VDNSSEPQNGYSPIASPESAESPRKRGKLSRSSSGNGTPRRTKL 139


>At1g32340.1 68414.m03985 zinc finger (C3HC4-type RING finger)
           family protein contains a Zinc finger, C3HC4 type (RING
           finger) signature, PROSITE:PS00518
          Length = 688

 Score = 28.3 bits (60), Expect = 3.7
 Identities = 17/50 (34%), Positives = 30/50 (60%), Gaps = 3/50 (6%)
 Frame = +3

Query: 261 ELRDNIKSEYHGKG--VLTAIKNINELIA-PELTKANLEVTQQREIDELM 401
           E+ D +  EY  K   +LT +K++   ++ PELT+  L++  Q + DEL+
Sbjct: 146 EIADGV-DEYETKEDIMLTILKDLRSSVSEPELTEEQLKMNDQLQEDELL 194


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 10,525,447
Number of Sequences: 28952
Number of extensions: 181942
Number of successful extensions: 467
Number of sequences better than 10.0: 5
Number of HSP's better than 10.0 without gapping: 452
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 462
length of database: 12,070,560
effective HSP length: 77
effective length of database: 9,841,256
effective search space used: 1062855648
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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