BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= NV060394.seq (558 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At1g74030.1 68414.m08573 enolase, putative similar to Swiss-Prot... 98 4e-21 At2g29560.1 68415.m03590 enolase, putative similar to enolase [S... 68 5e-12 At2g36530.1 68415.m04481 enolase identical to SWISS-PROT:P25696 ... 64 5e-11 At1g64050.1 68414.m07255 expressed protein 29 2.8 At1g32340.1 68414.m03985 zinc finger (C3HC4-type RING finger) fa... 28 3.7 >At1g74030.1 68414.m08573 enolase, putative similar to Swiss-Prot:P15007 enolase (EC 4.2.1.11) (2-phosphoglycerate dehydratase)(2-phospho-D- glycerate hydro-lyase) [Drosophila melanogaster] Length = 477 Score = 97.9 bits (233), Expect = 4e-21 Identities = 53/85 (62%), Positives = 60/85 (70%) Frame = +3 Query: 255 ALELRDNIKSEYHGKGVLTAIKNINELIAPELTKANLEVTQQREIDELMLKLDGTENKSK 434 ALELRD KS Y GKGVL AIKNINEL+AP+L ++V Q ++D LML+LDGT NKSK Sbjct: 93 ALELRDGDKSVYGGKGVLQAIKNINELVAPKLI--GVDVRNQADVDALMLELDGTPNKSK 150 Query: 435 LGANAILGVSLXXXXXXXXXXNVPL 509 LGANAILGVSL VPL Sbjct: 151 LGANAILGVSLSVCRAGAGAKGVPL 175 Score = 70.1 bits (164), Expect = 9e-13 Identities = 32/45 (71%), Positives = 40/45 (88%) Frame = +1 Query: 127 IKSIKARQIFDSRGNPTVEVDLVTELGLFRAAVPSGASTGVHELL 261 +K +KARQI DSRGNPTVEVDL+T+ L+R+AVPSGASTG++E L Sbjct: 51 VKGVKARQIIDSRGNPTVEVDLITD-DLYRSAVPSGASTGIYEAL 94 >At2g29560.1 68415.m03590 enolase, putative similar to enolase [Spinacia oleracea] gi|8919731|emb|CAB96173 Length = 475 Score = 67.7 bits (158), Expect = 5e-12 Identities = 39/85 (45%), Positives = 49/85 (57%) Frame = +3 Query: 255 ALELRDNIKSEYHGKGVLTAIKNINELIAPELTKANLEVTQQREIDELMLKLDGTENKSK 434 A+ELRD K Y G V A+KNINE I+ L ++ Q +ID+ M+ LD TE KS+ Sbjct: 90 AIELRDGDKGMYLGNSVAKAVKNINEKISEALI--GMDPKLQGQIDQAMIDLDKTEKKSE 147 Query: 435 LGANAILGVSLXXXXXXXXXXNVPL 509 LGANAIL VS+ VPL Sbjct: 148 LGANAILAVSIAACKAGAAEKEVPL 172 Score = 65.3 bits (152), Expect = 3e-11 Identities = 35/61 (57%), Positives = 44/61 (72%) Frame = +1 Query: 79 ITLNLRKSTSSVLKMVIKSIKARQIFDSRGNPTVEVDLVTELGLFRAAVPSGASTGVHEL 258 I +L+K+ SSV I +KARQI DSRG PTVEVDL T G+FRA+VPSG S+G +E Sbjct: 35 IANHLKKAVSSV----ITKVKARQILDSRGIPTVEVDLHTNKGVFRASVPSGDSSGTYEA 90 Query: 259 L 261 + Sbjct: 91 I 91 Score = 27.9 bits (59), Expect = 4.9 Identities = 11/31 (35%), Positives = 19/31 (61%) Frame = +2 Query: 464 LSCC*GWCCQEECSAYKHLADLAGNNDIVLP 556 ++ C ++E KHL+DL+G ++VLP Sbjct: 158 IAACKAGAAEKEVPLCKHLSDLSGRANMVLP 188 >At2g36530.1 68415.m04481 enolase identical to SWISS-PROT:P25696 enolase (EC 4.2.1.11) (2-phosphoglycerate dehydratase)(2-phospho-D- glycerate hydro-lyase) [Arabidopsis thaliana] Length = 444 Score = 64.5 bits (150), Expect = 5e-11 Identities = 43/91 (47%), Positives = 52/91 (57%), Gaps = 6/91 (6%) Frame = +3 Query: 255 ALELRDNIKSEYHGKGVLTAIKNINELIAPELTKANLEVTQQREIDELML-KLDGTEN-- 425 ALELRD S+Y GKGV A+ N+N +I P L + TQQ ID M+ +LDGT+N Sbjct: 48 ALELRDG-GSDYLGKGVSKAVGNVNNIIGPAL--IGKDPTQQTAIDNFMVHELDGTQNEW 104 Query: 426 ---KSKLGANAILGVSLXXXXXXXXXXNVPL 509 K KLGANAIL VSL +PL Sbjct: 105 GWCKQKLGANAILAVSLAVCKAGAVVSGIPL 135 Score = 63.7 bits (148), Expect = 8e-11 Identities = 32/46 (69%), Positives = 37/46 (80%), Gaps = 1/46 (2%) Frame = +1 Query: 127 IKSIKARQIFDSRGNPTVEVDLVTELGL-FRAAVPSGASTGVHELL 261 I +KARQIFDSRGNPTVEVD+ T G+ AAVPSGASTG++E L Sbjct: 4 ITVVKARQIFDSRGNPTVEVDIHTSNGIKVTAAVPSGASTGIYEAL 49 Score = 29.5 bits (63), Expect = 1.6 Identities = 12/16 (75%), Positives = 14/16 (87%) Frame = +2 Query: 509 YKHLADLAGNNDIVLP 556 YKH+A+LAGN IVLP Sbjct: 136 YKHIANLAGNPKIVLP 151 >At1g64050.1 68414.m07255 expressed protein Length = 668 Score = 28.7 bits (61), Expect = 2.8 Identities = 13/44 (29%), Positives = 26/44 (59%) Frame = -2 Query: 281 LDVISQFKSS*TPVDAPEGTAARNKPSSVTRSTSTVGLPRESKI 150 +D S+ ++ +P+ +PE + K ++RS+S G PR +K+ Sbjct: 96 VDNSSEPQNGYSPIASPESAESPRKRGKLSRSSSGNGTPRRTKL 139 >At1g32340.1 68414.m03985 zinc finger (C3HC4-type RING finger) family protein contains a Zinc finger, C3HC4 type (RING finger) signature, PROSITE:PS00518 Length = 688 Score = 28.3 bits (60), Expect = 3.7 Identities = 17/50 (34%), Positives = 30/50 (60%), Gaps = 3/50 (6%) Frame = +3 Query: 261 ELRDNIKSEYHGKG--VLTAIKNINELIA-PELTKANLEVTQQREIDELM 401 E+ D + EY K +LT +K++ ++ PELT+ L++ Q + DEL+ Sbjct: 146 EIADGV-DEYETKEDIMLTILKDLRSSVSEPELTEEQLKMNDQLQEDELL 194 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 10,525,447 Number of Sequences: 28952 Number of extensions: 181942 Number of successful extensions: 467 Number of sequences better than 10.0: 5 Number of HSP's better than 10.0 without gapping: 452 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 462 length of database: 12,070,560 effective HSP length: 77 effective length of database: 9,841,256 effective search space used: 1062855648 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
- SilkBase 1999-2023 -