BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= NV060393.seq (657 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBC215.09c |erg10||acetyl-CoA C-acetyltransferase Erg10 |Schizo... 64 2e-11 SPBC25B2.07c |mug164||microtubule-associated protein|Schizosacch... 28 1.0 SPAC2F3.10 |||GARP complex subunit Vps54 |Schizosaccharomyces po... 27 2.4 SPAC17C9.03 |tif471||translation initiation factor eIF4G |Schizo... 27 3.1 SPAC222.09 |seb1||RNA-binding protein Seb1 |Schizosaccharomyces ... 27 3.1 SPBC17G9.07 |rps2402|rps24-2|40S ribosomal protein S24|Schizosac... 26 5.5 SPCC962.01 ||SPCP31B10.09|C2 domain protein|Schizosaccharomyces ... 25 7.3 SPBC146.01 |med15|SPBP35G2.15|mediator complex subunit Med15 |Sc... 25 7.3 SPAC694.06c |mrc1||mediator of replication checkpoint 1 |Schizos... 25 7.3 SPBC1289.10c |||transcription factor |Schizosaccharomyces pombe|... 25 9.6 SPCC777.04 |||amino acid transporter |Schizosaccharomyces pombe|... 25 9.6 >SPBC215.09c |erg10||acetyl-CoA C-acetyltransferase Erg10 |Schizosaccharomyces pombe|chr 2|||Manual Length = 395 Score = 63.7 bits (148), Expect = 2e-11 Identities = 34/84 (40%), Positives = 49/84 (58%) Frame = +1 Query: 250 GPVISSSQTDGIYTPRHAALKAGIPQEKPVLGINRLCGSGFQSIVNSAQDILTGAAKISV 429 G V+S++ G R AL AG+P+ +N++C SG ++ + AQ I+TG A+I V Sbjct: 56 GNVVSANL--GQNPARQCALGAGLPRSIVCTTVNKVCASGMKATILGAQTIMTGNAEIVV 113 Query: 430 AGGVENMSQAPFAVRNVRFGTALG 501 AGG E+MS AP+ RFG G Sbjct: 114 AGGTESMSNAPYYAPKNRFGAKYG 137 Score = 52.8 bits (121), Expect = 4e-08 Identities = 23/51 (45%), Positives = 34/51 (66%) Frame = +2 Query: 101 IFIVGAKRTPFGTFGGVFRNTSATELQTIATVGALKEAGVSPAQVDSMVVG 253 ++IV A RTP G+FGG F + AT+L +IA GAL+ + P+ VD + +G Sbjct: 6 VYIVSAVRTPMGSFGGSFASLPATKLGSIAIKGALERVNIKPSDVDEVFMG 56 Score = 32.3 bits (70), Expect = 0.063 Identities = 17/39 (43%), Positives = 22/39 (56%), Gaps = 2/39 (5%) Frame = +3 Query: 534 GLSDSYCGLPMGMTAEKLGAQFGITR--DESLTITLYNR 644 GLSD+Y GLPMG AE + I R ++ I+ Y R Sbjct: 149 GLSDAYDGLPMGNAAELCAEEHSIDRASQDAFAISSYKR 187 >SPBC25B2.07c |mug164||microtubule-associated protein|Schizosaccharomyces pombe|chr 2|||Manual Length = 501 Score = 28.3 bits (60), Expect = 1.0 Identities = 14/27 (51%), Positives = 17/27 (62%) Frame = +1 Query: 478 VRFGTALGTNSPSKTPSGPDSRTPTAD 558 VR GT+LG SPS+ S SRT T + Sbjct: 355 VRPGTSLGVRSPSRIQSTLSSRTTTGN 381 >SPAC2F3.10 |||GARP complex subunit Vps54 |Schizosaccharomyces pombe|chr 1|||Manual Length = 949 Score = 27.1 bits (57), Expect = 2.4 Identities = 11/52 (21%), Positives = 27/52 (51%), Gaps = 1/52 (1%) Frame = -1 Query: 423 DLGCAGQNVLSAVHDRLE-TRAA*SVDAEHRLLLRNTSFERRVTWRVDSVSL 271 D + V + ++ T+ + + ++ LLL N+ + +++W +D+V L Sbjct: 177 DFNLDDPQIFDVVSEHIDITQTSDAPNSNRNLLLNNSMLQEKISWYLDTVEL 228 >SPAC17C9.03 |tif471||translation initiation factor eIF4G |Schizosaccharomyces pombe|chr 1|||Manual Length = 1403 Score = 26.6 bits (56), Expect = 3.1 Identities = 21/61 (34%), Positives = 24/61 (39%) Frame = +3 Query: 414 SQDLSSGGSREHVASALRGQKREVRHGSGHQLAFEDTLWAGLSDSYCGLPMGMTAEKLGA 593 SQ SSG R H RG R G G F + W+ D Y L G+ K G Sbjct: 1275 SQRPSSG--RMHGRDMNRGDSR--MGGRGSNPPFSSSDWSNNKDGYARLGQGIRGLKSGT 1330 Query: 594 Q 596 Q Sbjct: 1331 Q 1331 >SPAC222.09 |seb1||RNA-binding protein Seb1 |Schizosaccharomyces pombe|chr 1|||Manual Length = 620 Score = 26.6 bits (56), Expect = 3.1 Identities = 13/40 (32%), Positives = 20/40 (50%) Frame = +2 Query: 128 PFGTFGGVFRNTSATELQTIATVGALKEAGVSPAQVDSMV 247 P G G + + T+ Q I + L V PAQ+DS++ Sbjct: 270 PSGPMGMKTGSVNDTQSQQITLMNVLASQNVPPAQIDSIM 309 >SPBC17G9.07 |rps2402|rps24-2|40S ribosomal protein S24|Schizosaccharomyces pombe|chr 2|||Manual Length = 134 Score = 25.8 bits (54), Expect = 5.5 Identities = 16/57 (28%), Positives = 28/57 (49%) Frame = +1 Query: 184 DSHCRCAEGGRSVPGSGRQHGRGPVISSSQTDGIYTPRHAALKAGIPQEKPVLGINR 354 DS C A G R+ G GR G + S+++ + P + ++ G Q +P+ + R Sbjct: 51 DSECVQAFGLRTHFGGGRSTGFALIYDSTESMKKFEPHYRLVRVG--QAEPIQKVAR 105 >SPCC962.01 ||SPCP31B10.09|C2 domain protein|Schizosaccharomyces pombe|chr 3|||Manual Length = 1429 Score = 25.4 bits (53), Expect = 7.3 Identities = 13/35 (37%), Positives = 19/35 (54%) Frame = +1 Query: 166 RDRTADDSHCRCAEGGRSVPGSGRQHGRGPVISSS 270 R +D S R +V SGR+HGR P+ S++ Sbjct: 495 RGEVSDSSIDRKPSSFITVTTSGREHGRTPIRSNT 529 >SPBC146.01 |med15|SPBP35G2.15|mediator complex subunit Med15 |Schizosaccharomyces pombe|chr 2|||Manual Length = 1063 Score = 25.4 bits (53), Expect = 7.3 Identities = 12/46 (26%), Positives = 23/46 (50%) Frame = -1 Query: 174 SVADVLRKTPPKVPNGVLLAPTMKIPLTEADMIQNYTYPVNVELKY 37 SV+ +R +PN P P +A+ + N ++PV+ +L + Sbjct: 755 SVSSNVRPINGSIPNAQPSVPGQAQPAAKANSVGNTSFPVDSKLAF 800 >SPAC694.06c |mrc1||mediator of replication checkpoint 1 |Schizosaccharomyces pombe|chr 1|||Manual Length = 1019 Score = 25.4 bits (53), Expect = 7.3 Identities = 15/59 (25%), Positives = 28/59 (47%) Frame = +2 Query: 158 NTSATELQTIATVGALKEAGVSPAQVDSMVVGQSSLHHKLTESTRHVTRRSKLVFLKRS 334 NTS+T+ + ++ + P Q+DS+ + ++ E R +RR FL R+ Sbjct: 630 NTSSTQPSQVDSLVPTQLDSTIPTQIDSVQRNKDQDDEEILEERRE-SRRDSKTFLSRT 687 >SPBC1289.10c |||transcription factor |Schizosaccharomyces pombe|chr 2|||Manual Length = 743 Score = 25.0 bits (52), Expect = 9.6 Identities = 21/65 (32%), Positives = 31/65 (47%), Gaps = 1/65 (1%) Frame = +1 Query: 214 RSVPGSGRQHGRGPVISSSQTDGIYTPRHAALKAG-IPQEKPVLGINRLCGSGFQSIVNS 390 RS+P S + G P S++Q + P A G +PQ P+ L G + + NS Sbjct: 313 RSIPSSPKTEG-AP--SNAQ----FRPSLPATPNGSVPQSNPLYDTTGLNGGQYPVVQNS 365 Query: 391 AQDIL 405 AQ +L Sbjct: 366 AQPLL 370 >SPCC777.04 |||amino acid transporter |Schizosaccharomyces pombe|chr 3|||Manual Length = 521 Score = 25.0 bits (52), Expect = 9.6 Identities = 6/13 (46%), Positives = 12/13 (92%) Frame = +1 Query: 52 VNWIGIILNHVCF 90 +NWI I+L+++C+ Sbjct: 421 INWINILLSYICY 433 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,523,286 Number of Sequences: 5004 Number of extensions: 49150 Number of successful extensions: 132 Number of sequences better than 10.0: 11 Number of HSP's better than 10.0 without gapping: 119 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 132 length of database: 2,362,478 effective HSP length: 70 effective length of database: 2,012,198 effective search space used: 297805304 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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