Bombyx BLAST Result against spombe

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= NV060381.seq
         (564 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces...   142   4e-35
SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|ch...    92   6e-20
SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosacchar...    91   1e-19
SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|...    73   4e-14
SPBC19G7.16 |iws1||transcription elongation factor complex subun...    27   1.9  
SPAC29E6.01 |pof11|SPAC30.05, mug156|F-box protein Pof11|Schizos...    27   2.5  
SPAC6C3.08 |||gankyrin|Schizosaccharomyces pombe|chr 1|||Manual        26   3.3  
SPBC15D4.03 |slm9||hira protein Slm9|Schizosaccharomyces pombe|c...    26   3.3  
SPAC23A1.19c ||SPAC26H5.01c|RecQ type DNA helicase Hrq1 |Schizos...    26   4.4  
SPBC646.12c |gap1|src1, sar1|GTPase activating protein Gap1|Schi...    25   5.8  
SPAC8F11.09c |nnt1||nicotinamide N-methyltransferase Nnt1 |Schiz...    25   5.8  
SPBC11C11.11c ||SPBC3B8.12|ATP-dependent DNA helicase Irc3 |Schi...    25   7.7  

>SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 448

 Score =  142 bits (343), Expect = 4e-35
 Identities = 61/76 (80%), Positives = 70/76 (92%)
 Frame = +2

Query: 257 RAILVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVR 436
           RA+LVDLEPGTMD+V+SG FG +FRPDN ++GQSGAGN WAKGHYTEGAEL D+VLDVVR
Sbjct: 62  RAVLVDLEPGTMDAVKSGKFGNLFRPDNIIYGQSGAGNIWAKGHYTEGAELADAVLDVVR 121

Query: 437 KEAESCDCLQGFQLTH 484
           +EAE+CD LQGFQLTH
Sbjct: 122 REAEACDALQGFQLTH 137



 Score =  106 bits (254), Expect = 3e-24
 Identities = 44/60 (73%), Positives = 51/60 (85%)
 Frame = +3

Query: 75  MREIVHIQAGQCGNQIGAKFWEIISDEHGIDPTGAYHGDSDLQLERINVYYNEASGGKYV 254
           MREIVHIQAGQCGNQ+GA FW  I+DEHG+D  G YHG S+ Q ER+NVY+NEA+GGKYV
Sbjct: 1   MREIVHIQAGQCGNQVGAAFWSTIADEHGLDSAGIYHGTSEAQHERLNVYFNEAAGGKYV 60



 Score = 26.2 bits (55), Expect = 3.3
 Identities = 10/11 (90%), Positives = 11/11 (100%)
 Frame = +3

Query: 531 KIREEYPDRIM 563
           KIREEYPDR+M
Sbjct: 154 KIREEYPDRMM 164


>SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 455

 Score = 91.9 bits (218), Expect = 6e-20
 Identities = 38/76 (50%), Positives = 55/76 (72%)
 Frame = +2

Query: 257 RAILVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVR 436
           R+I VDLEP  +D VR+GP+  +F P+  V G+  A NN+A+GHYT G E++DSVL+ +R
Sbjct: 68  RSIYVDLEPNVIDQVRTGPYKDLFHPEQMVTGKEDASNNYARGHYTVGKEMIDSVLERIR 127

Query: 437 KEAESCDCLQGFQLTH 484
           + A++C  LQGF + H
Sbjct: 128 RMADNCSGLQGFLVFH 143



 Score = 44.4 bits (100), Expect = 1e-05
 Identities = 22/56 (39%), Positives = 31/56 (55%)
 Frame = +3

Query: 75  MREIVHIQAGQCGNQIGAKFWEIISDEHGIDPTGAYHGDSDLQLERINVYYNEASG 242
           MRE++ +  GQ G QIG   WE+   EHGI P G    +S  ++ + N Y N+  G
Sbjct: 1   MREVISVHVGQAGVQIGNACWELYCLEHGIGPDGFPTENS--EVHKNNSYLNDGFG 54


>SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha
           2|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 449

 Score = 90.6 bits (215), Expect = 1e-19
 Identities = 38/76 (50%), Positives = 53/76 (69%)
 Frame = +2

Query: 257 RAILVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVR 436
           R+I VDLEP  +D VR+GP+  +F P+  + G+  A NN+A+GHYT G ELVD V D +R
Sbjct: 64  RSIYVDLEPNVIDQVRTGPYRDLFHPEQLITGKEDASNNYARGHYTVGKELVDEVTDKIR 123

Query: 437 KEAESCDCLQGFQLTH 484
           + A++C  LQGF + H
Sbjct: 124 RIADNCSGLQGFLVFH 139



 Score = 50.4 bits (115), Expect = 2e-07
 Identities = 25/62 (40%), Positives = 34/62 (54%), Gaps = 2/62 (3%)
 Frame = +3

Query: 75  MREIVHIQAGQCGNQIGAKFWEIISDEHGIDPTGAYHGDSDLQLE--RINVYYNEASGGK 248
           MREI+ I  GQ G QIG   WE+   EHGI P G  + ++  Q      + +++E   GK
Sbjct: 1   MREIISIHVGQAGTQIGNACWELYCLEHGIQPNGYMNPETASQNSDGGFSTFFSETGQGK 60

Query: 249 YV 254
           YV
Sbjct: 61  YV 62


>SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 446

 Score = 72.5 bits (170), Expect = 4e-14
 Identities = 30/78 (38%), Positives = 52/78 (66%), Gaps = 2/78 (2%)
 Frame = +2

Query: 257 RAILVDLEPGTMDSVRSGPFGQIFRPDNFVFGQS--GAGNNWAKGHYTEGAELVDSVLDV 430
           RAIL+DLEP  ++++ S  +G ++ P+N +  ++  GAGNNWA G Y+    + + ++D+
Sbjct: 63  RAILIDLEPRVVNNILSDTYGSLYNPENILITKNGGGAGNNWANG-YSHAERIFEDIMDM 121

Query: 431 VRKEAESCDCLQGFQLTH 484
           + +EA+  D L+GF L H
Sbjct: 122 IDREADGSDSLEGFSLLH 139



 Score = 60.1 bits (139), Expect = 2e-10
 Identities = 24/59 (40%), Positives = 40/59 (67%)
 Frame = +3

Query: 78  REIVHIQAGQCGNQIGAKFWEIISDEHGIDPTGAYHGDSDLQLERINVYYNEASGGKYV 254
           REI+ +QAGQCGNQIG++FW+ +  EHGI P G     +   ++R +V++ ++   +Y+
Sbjct: 3   REIITLQAGQCGNQIGSQFWQQLCLEHGIGPDGTLESFATEGVDRKDVFFYQSDDTRYI 61


>SPBC19G7.16 |iws1||transcription elongation factor complex subunit
           Iws1 |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 428

 Score = 27.1 bits (57), Expect = 1.9
 Identities = 10/34 (29%), Positives = 22/34 (64%)
 Frame = +3

Query: 114 NQIGAKFWEIISDEHGIDPTGAYHGDSDLQLERI 215
           N++G    E+++++  +DPT A   + DLQ++ +
Sbjct: 133 NELGENEEEVLTEQKQLDPTLAAKKELDLQMDAV 166


>SPAC29E6.01 |pof11|SPAC30.05, mug156|F-box protein
           Pof11|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 506

 Score = 26.6 bits (56), Expect = 2.5
 Identities = 14/35 (40%), Positives = 19/35 (54%), Gaps = 1/35 (2%)
 Frame = +2

Query: 308 GPFGQIFRPDNFVFGQSGAG-NNWAKGHYTEGAEL 409
           GP+G +F P  F+F  +G    NW+   Y E A L
Sbjct: 157 GPYGTMFLPQQFIFDSNGRPLLNWSY-LYKEHAHL 190


>SPAC6C3.08 |||gankyrin|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 234

 Score = 26.2 bits (55), Expect = 3.3
 Identities = 12/30 (40%), Positives = 19/30 (63%)
 Frame = +2

Query: 377 AKGHYTEGAELVDSVLDVVRKEAESCDCLQ 466
           A+GH   G ELV +  D +RK++E+   L+
Sbjct: 183 AEGHPDVGVELVRAGADTLRKDSENHTALE 212


>SPBC15D4.03 |slm9||hira protein Slm9|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 807

 Score = 26.2 bits (55), Expect = 3.3
 Identities = 16/46 (34%), Positives = 23/46 (50%)
 Frame = -1

Query: 450 DSASFRTTSKTESTSSAPSV*CPLAQLLPAPDCPKTKLSGRKICPK 313
           ++A+ RTTS T+    +PS       L P P    +  S R+ CPK
Sbjct: 390 NAAADRTTSPTQGQPESPS---KSILLRPPPSIASSPESKRRKCPK 432


>SPAC23A1.19c ||SPAC26H5.01c|RecQ type DNA helicase Hrq1
            |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 1063

 Score = 25.8 bits (54), Expect = 4.4
 Identities = 12/44 (27%), Positives = 20/44 (45%)
 Frame = +2

Query: 329  RPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKEAESCDC 460
            RP   +F     G++   G   +  E  D ++ +  +  ESCDC
Sbjct: 955  RPSRLIF-YDNCGDSSGAGLCNKAYEHTDELITMAIERIESCDC 997


>SPBC646.12c |gap1|src1, sar1|GTPase activating protein
           Gap1|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 766

 Score = 25.4 bits (53), Expect = 5.8
 Identities = 15/46 (32%), Positives = 27/46 (58%)
 Frame = -2

Query: 227 VVYIDALQLQVRVPMVSTGGVDAVLVGDDLPELSSDLVAALTSLDM 90
           ++Y+DA  L +++  +   G  A  V  DLP L +D V++L S+ +
Sbjct: 511 ILYVDAKTLFIQLLRLLPSGHPATRVPLDLP-LIADSVSSLKSMSL 555


>SPAC8F11.09c |nnt1||nicotinamide N-methyltransferase Nnt1
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 255

 Score = 25.4 bits (53), Expect = 5.8
 Identities = 13/32 (40%), Positives = 16/32 (50%)
 Frame = -1

Query: 366 PAPDCPKTKLSGRKICPKGPERTESMVPGSKS 271
           P+   PK  L  R I P GPE  +  + GS S
Sbjct: 20  PSTPPPKEVLHTRVIVPNGPEEIKLRLVGSHS 51


>SPBC11C11.11c ||SPBC3B8.12|ATP-dependent DNA helicase Irc3
           |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 606

 Score = 25.0 bits (52), Expect = 7.7
 Identities = 17/39 (43%), Positives = 22/39 (56%)
 Frame = -3

Query: 415 IDELSTLRVVSLGPVVTGAGLSEDEVVRTEDLSERSRAD 299
           +D   +LR VSL    T AGLS DEV    + S+ S A+
Sbjct: 369 LDFCDSLRRVSLHVDPTLAGLSPDEVENFYNKSKNSLAN 407


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,103,553
Number of Sequences: 5004
Number of extensions: 38438
Number of successful extensions: 117
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 108
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 116
length of database: 2,362,478
effective HSP length: 69
effective length of database: 2,017,202
effective search space used: 238029836
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)