Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= NV060378.seq
(666 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At2g36460.1 68415.m04475 fructose-bisphosphate aldolase, putativ... 90 1e-18
At4g26520.1 68417.m03820 fructose-bisphosphate aldolase, cytopla... 90 1e-18
At2g21330.1 68415.m02538 fructose-bisphosphate aldolase, putativ... 88 4e-18
At4g26530.1 68417.m03822 fructose-bisphosphate aldolase, putativ... 86 2e-17
At4g38970.2 68417.m05522 fructose-bisphosphate aldolase, putativ... 85 3e-17
At4g38970.1 68417.m05521 fructose-bisphosphate aldolase, putativ... 85 3e-17
At3g52930.1 68416.m05834 fructose-bisphosphate aldolase, putativ... 85 5e-17
At2g01140.1 68415.m00023 fructose-bisphosphate aldolase, putativ... 84 9e-17
At5g03690.2 68418.m00329 fructose-bisphosphate aldolase, putativ... 80 1e-15
At5g03690.1 68418.m00328 fructose-bisphosphate aldolase, putativ... 80 1e-15
At2g29980.2 68415.m03647 omega-3 fatty acid desaturase, endoplas... 31 0.91
At2g29980.1 68415.m03646 omega-3 fatty acid desaturase, endoplas... 31 0.91
At1g15550.1 68414.m01870 gibberellin 3-beta-dioxygenase / gibber... 29 3.7
At5g19460.1 68418.m02319 MutT/nudix family protein similar to SP... 28 6.4
At3g27925.1 68416.m03484 DegP protease, putative SP:022609; almo... 27 8.5
>At2g36460.1 68415.m04475 fructose-bisphosphate aldolase, putative
similar to PIR|S65073 fructose-bisphosphate aldolase (EC
4.1.2.13) isoenzyme C-1, cytosolic [Oryza sativa];
contains Pfam profile PF00274 Fructose-bisphosphate
aldolase class-I
Length = 358
Score = 90.2 bits (214), Expect = 1e-18
Identities = 44/76 (57%), Positives = 54/76 (71%)
Frame = +2
Query: 278 LQDIGVENTEENRRRYRQLLFSSDAVLSENISGVILFHETLYQKADDGTPLVSLLEKKGI 457
L I VEN E NRR R+LLF++ L +SGVILF ETLYQK+ DGTP V +L+ G+
Sbjct: 40 LASINVENVESNRRALRELLFTTPGALP-CLSGVILFEETLYQKSSDGTPFVDMLKSAGV 98
Query: 458 IPGIKVDKGVVPLFGS 505
+PGIKVDKG V L G+
Sbjct: 99 LPGIKVDKGTVELAGT 114
Score = 52.4 bits (120), Expect = 3e-07
Identities = 28/51 (54%), Positives = 30/51 (58%), Gaps = 1/51 (1%)
Frame = +1
Query: 511 ECTTQGLDDLASACAQYKKDGCHFAKWRCVLKIG-RTPLVQAXQENANVLA 660
E TTQGLD L C +Y + G FAKWR VLKIG P A ENA LA
Sbjct: 117 ETTTQGLDGLGDRCKKYYEAGARFAKWRAVLKIGVNEPSQLAIHENAYGLA 167
>At4g26520.1 68417.m03820 fructose-bisphosphate aldolase,
cytoplasmic identical to SP|P22197 Fructose-bisphosphate
aldolase, cytoplasmic isozyme (EC 4.1.2.13) {Arabidopsis
thaliana}
Length = 358
Score = 89.8 bits (213), Expect = 1e-18
Identities = 45/73 (61%), Positives = 53/73 (72%)
Frame = +2
Query: 287 IGVENTEENRRRYRQLLFSSDAVLSENISGVILFHETLYQKADDGTPLVSLLEKKGIIPG 466
I VENTE NR+ YR+LLF+S +SGVILF ETLYQK DG P V LL + G+IPG
Sbjct: 43 INVENTESNRQAYRELLFTSPGSYP-CLSGVILFEETLYQKTSDGKPFVDLLMENGVIPG 101
Query: 467 IKVDKGVVPLFGS 505
IKVDKG+V L G+
Sbjct: 102 IKVDKGLVDLAGT 114
Score = 58.4 bits (135), Expect = 4e-09
Identities = 33/73 (45%), Positives = 39/73 (53%), Gaps = 1/73 (1%)
Frame = +1
Query: 445 EEGHHPRHQGRQGCRPAVRIGNECTTQGLDDLASACAQYKKDGCHFAKWRCVLKIGRT-P 621
E G P + +G E TTQGLD L + C QY + G FAKWR KIG T P
Sbjct: 95 ENGVIPGIKVDKGLVDLAGTNGETTTQGLDSLGARCQQYYEAGARFAKWRAFFKIGATEP 154
Query: 622 LVQAXQENANVLA 660
V + QE+A VLA
Sbjct: 155 SVLSIQEDARVLA 167
Score = 29.5 bits (63), Expect = 2.1
Identities = 13/23 (56%), Positives = 17/23 (73%)
Frame = +3
Query: 186 QEELKKIAQAIVAPAKGILAADE 254
++EL K A+ I P +GILAADE
Sbjct: 9 EDELIKTAKYIATPGRGILAADE 31
>At2g21330.1 68415.m02538 fructose-bisphosphate aldolase, putative
strong similarity to plastidic fructose-bisphosphate
aldolase (EC 4.1.2.13) from Nicotiana paniculata
(NPALDP1) [GI:4827251], Oryza sativa, PIR2:T02057
[SP|Q40677]
Length = 399
Score = 88.2 bits (209), Expect = 4e-18
Identities = 44/76 (57%), Positives = 54/76 (71%)
Frame = +2
Query: 278 LQDIGVENTEENRRRYRQLLFSSDAVLSENISGVILFHETLYQKADDGTPLVSLLEKKGI 457
L IG+ENTE NR+ YR LL S+ L + ISG ILF ETLYQ DG +V +L ++ I
Sbjct: 84 LASIGLENTEANRQAYRTLLVSAPG-LGQYISGAILFEETLYQSTTDGKKMVDVLVEQNI 142
Query: 458 IPGIKVDKGVVPLFGS 505
+PGIKVDKG+VPL GS
Sbjct: 143 VPGIKVDKGLVPLVGS 158
Score = 45.6 bits (103), Expect = 3e-05
Identities = 27/61 (44%), Positives = 32/61 (52%)
Frame = +1
Query: 478 QGCRPAVRIGNECTTQGLDDLASACAQYKKDGCHFAKWRCVLKIGRTPLVQAXQENANVL 657
+G P V +E QGLD LAS A Y + G FAKWR V+ I P A +E A L
Sbjct: 150 KGLVPLVGSYDESWCQGLDGLASRTAAYYQQGARFAKWRTVVSIPNGPSALAVKEAAWGL 209
Query: 658 A 660
A
Sbjct: 210 A 210
>At4g26530.1 68417.m03822 fructose-bisphosphate aldolase, putative
strong similarity to SP|P22197 Fructose-bisphosphate
aldolase, cytoplasmic isozyme (EC 4.1.2.13) {Arabidopsis
thaliana}
Length = 358
Score = 86.2 bits (204), Expect = 2e-17
Identities = 44/73 (60%), Positives = 51/73 (69%)
Frame = +2
Query: 287 IGVENTEENRRRYRQLLFSSDAVLSENISGVILFHETLYQKADDGTPLVSLLEKKGIIPG 466
I VEN E NR+ R+LLF+S +SGVILF ETLYQK DG P V LL + G+IPG
Sbjct: 43 INVENIESNRQALRELLFTSPGTFP-CLSGVILFEETLYQKTTDGKPFVELLMENGVIPG 101
Query: 467 IKVDKGVVPLFGS 505
IKVDKGVV L G+
Sbjct: 102 IKVDKGVVDLAGT 114
Score = 59.3 bits (137), Expect = 2e-09
Identities = 34/73 (46%), Positives = 39/73 (53%), Gaps = 1/73 (1%)
Frame = +1
Query: 445 EEGHHPRHQGRQGCRPAVRIGNECTTQGLDDLASACAQYKKDGCHFAKWRCVLKIGRT-P 621
E G P + +G E TTQGLD L + C +Y K G FAKWR VLKIG T P
Sbjct: 95 ENGVIPGIKVDKGVVDLAGTNGETTTQGLDSLGARCQEYYKAGARFAKWRAVLKIGATEP 154
Query: 622 LVQAXQENANVLA 660
+ QENA LA
Sbjct: 155 SELSIQENAKGLA 167
Score = 29.9 bits (64), Expect = 1.6
Identities = 14/22 (63%), Positives = 16/22 (72%)
Frame = +3
Query: 189 EELKKIAQAIVAPAKGILAADE 254
+EL K A+ I P KGILAADE
Sbjct: 10 DELIKTAKYIATPGKGILAADE 31
>At4g38970.2 68417.m05522 fructose-bisphosphate aldolase, putative
strong similarity to plastidic fructose-bisphosphate
aldolase (EC 4.1.2.13) from Nicotiana paniculata
(NPALDP1) [GI:4827251], Oryza sativa, PIR2:T02057
[SP|Q40677]
Length = 381
Score = 85.4 bits (202), Expect = 3e-17
Identities = 41/80 (51%), Positives = 55/80 (68%)
Frame = +2
Query: 278 LQDIGVENTEENRRRYRQLLFSSDAVLSENISGVILFHETLYQKADDGTPLVSLLEKKGI 457
L IG+ENTE NR+ +R LL S+ L + +SG ILF ETLYQ +G +V +L ++ I
Sbjct: 83 LDSIGLENTEANRQAFRTLLVSAPG-LGQYVSGAILFEETLYQSTTEGKKMVDVLVEQNI 141
Query: 458 IPGIKVDKGVVPLFGSETNA 517
+PGIKVDKG+VPL GS +
Sbjct: 142 VPGIKVDKGLVPLVGSNNES 161
Score = 47.6 bits (108), Expect = 7e-06
Identities = 27/61 (44%), Positives = 32/61 (52%)
Frame = +1
Query: 478 QGCRPAVRIGNECTTQGLDDLASACAQYKKDGCHFAKWRCVLKIGRTPLVQAXQENANVL 657
+G P V NE QGLD L+S A Y + G FAKWR V+ I P A +E A L
Sbjct: 149 KGLVPLVGSNNESWCQGLDGLSSRTAAYYQQGARFAKWRTVVSIPNGPSALAVKEAAWGL 208
Query: 658 A 660
A
Sbjct: 209 A 209
>At4g38970.1 68417.m05521 fructose-bisphosphate aldolase, putative
strong similarity to plastidic fructose-bisphosphate
aldolase (EC 4.1.2.13) from Nicotiana paniculata
(NPALDP1) [GI:4827251], Oryza sativa, PIR2:T02057
[SP|Q40677]
Length = 398
Score = 85.4 bits (202), Expect = 3e-17
Identities = 41/80 (51%), Positives = 55/80 (68%)
Frame = +2
Query: 278 LQDIGVENTEENRRRYRQLLFSSDAVLSENISGVILFHETLYQKADDGTPLVSLLEKKGI 457
L IG+ENTE NR+ +R LL S+ L + +SG ILF ETLYQ +G +V +L ++ I
Sbjct: 83 LDSIGLENTEANRQAFRTLLVSAPG-LGQYVSGAILFEETLYQSTTEGKKMVDVLVEQNI 141
Query: 458 IPGIKVDKGVVPLFGSETNA 517
+PGIKVDKG+VPL GS +
Sbjct: 142 VPGIKVDKGLVPLVGSNNES 161
Score = 47.6 bits (108), Expect = 7e-06
Identities = 27/61 (44%), Positives = 32/61 (52%)
Frame = +1
Query: 478 QGCRPAVRIGNECTTQGLDDLASACAQYKKDGCHFAKWRCVLKIGRTPLVQAXQENANVL 657
+G P V NE QGLD L+S A Y + G FAKWR V+ I P A +E A L
Sbjct: 149 KGLVPLVGSNNESWCQGLDGLSSRTAAYYQQGARFAKWRTVVSIPNGPSALAVKEAAWGL 208
Query: 658 A 660
A
Sbjct: 209 A 209
>At3g52930.1 68416.m05834 fructose-bisphosphate aldolase, putative
similar to SP|O65735|ALF_CICAR Fructose-bisphosphate
aldolase, cytoplasmic isozyme {Cicer arietinum},
cytosolic aldolase [Fragaria x ananassa] GI:10645188;
contains Pfam profile PF00274 Fructose-bisphosphate
aldolase class-I
Length = 358
Score = 84.6 bits (200), Expect = 5e-17
Identities = 42/77 (54%), Positives = 54/77 (70%)
Frame = +2
Query: 278 LQDIGVENTEENRRRYRQLLFSSDAVLSENISGVILFHETLYQKADDGTPLVSLLEKKGI 457
L I VEN E NRR R+LLF++ L +SGVILF ETLYQK+ DG V +L++ G+
Sbjct: 40 LASINVENVETNRRNLRELLFTAPGALP-CLSGVILFEETLYQKSSDGKLFVDILKEGGV 98
Query: 458 IPGIKVDKGVVPLFGSE 508
+PGIKVDKG V L G++
Sbjct: 99 LPGIKVDKGTVELAGTD 115
Score = 52.4 bits (120), Expect = 3e-07
Identities = 27/51 (52%), Positives = 30/51 (58%), Gaps = 1/51 (1%)
Frame = +1
Query: 511 ECTTQGLDDLASACAQYKKDGCHFAKWRCVLKIGRT-PLVQAXQENANVLA 660
E TTQGLD L C +Y + G FAKWR VLKIG P + ENA LA
Sbjct: 117 ETTTQGLDGLGDRCKKYYEAGARFAKWRAVLKIGENEPSEHSIHENAYGLA 167
>At2g01140.1 68415.m00023 fructose-bisphosphate aldolase, putative
similar to plastidic aldolase NPALDP1 from Nicotiana
paniculata [GI:4827251]; contains Pfam profile PF00274
Fructose-bisphosphate aldolase class-I
Length = 391
Score = 83.8 bits (198), Expect = 9e-17
Identities = 42/76 (55%), Positives = 51/76 (67%)
Frame = +2
Query: 278 LQDIGVENTEENRRRYRQLLFSSDAVLSENISGVILFHETLYQKADDGTPLVSLLEKKGI 457
L IG++NTE+NR+ YRQLL ++ L + ISG ILF ETLYQ DG V L I
Sbjct: 76 LASIGLDNTEDNRQAYRQLLLTTPG-LGDYISGSILFEETLYQSTKDGKTFVDCLRDANI 134
Query: 458 IPGIKVDKGVVPLFGS 505
+PGIKVDKG+ PL GS
Sbjct: 135 VPGIKVDKGLSPLAGS 150
Score = 46.8 bits (106), Expect = 1e-05
Identities = 26/61 (42%), Positives = 31/61 (50%)
Frame = +1
Query: 478 QGCRPAVRIGNECTTQGLDDLASACAQYKKDGCHFAKWRCVLKIGRTPLVQAXQENANVL 657
+G P E QGLD LAS A+Y K G FAKWR V+ + P A +E A L
Sbjct: 142 KGLSPLAGSNEESWCQGLDGLASRSAEYYKQGARFAKWRTVVSVPCGPSALAVKEAAWGL 201
Query: 658 A 660
A
Sbjct: 202 A 202
>At5g03690.2 68418.m00329 fructose-bisphosphate aldolase, putative
similar to PIR|S65073 fructose-bisphosphate aldolase (EC
4.1.2.13) isoenzyme C-1, cytosolic [Oryza sativa];
contains Pfam profile PF00274 Fructose-bisphosphate
aldolase class-I
Length = 359
Score = 80.2 bits (189), Expect = 1e-15
Identities = 39/73 (53%), Positives = 51/73 (69%)
Frame = +2
Query: 287 IGVENTEENRRRYRQLLFSSDAVLSENISGVILFHETLYQKADDGTPLVSLLEKKGIIPG 466
I VEN E NRR R+LLF++ L + ISG+ILF ETLYQK G V ++++ G++PG
Sbjct: 43 INVENVESNRRALRELLFTTPGAL-QYISGIILFEETLYQKTASGKLFVDVMKEAGVLPG 101
Query: 467 IKVDKGVVPLFGS 505
IKVDKG V L G+
Sbjct: 102 IKVDKGTVELAGT 114
Score = 50.4 bits (115), Expect = 1e-06
Identities = 27/51 (52%), Positives = 29/51 (56%), Gaps = 1/51 (1%)
Frame = +1
Query: 511 ECTTQGLDDLASACAQYKKDGCHFAKWRCVLKIGRT-PLVQAXQENANVLA 660
E TT GLD L C +Y + G FAKWR VLKIG P A ENA LA
Sbjct: 117 ETTTTGLDGLGDRCKKYYEAGARFAKWRAVLKIGNNEPSELAIHENAYGLA 167
>At5g03690.1 68418.m00328 fructose-bisphosphate aldolase, putative
similar to PIR|S65073 fructose-bisphosphate aldolase (EC
4.1.2.13) isoenzyme C-1, cytosolic [Oryza sativa];
contains Pfam profile PF00274 Fructose-bisphosphate
aldolase class-I
Length = 393
Score = 80.2 bits (189), Expect = 1e-15
Identities = 39/73 (53%), Positives = 51/73 (69%)
Frame = +2
Query: 287 IGVENTEENRRRYRQLLFSSDAVLSENISGVILFHETLYQKADDGTPLVSLLEKKGIIPG 466
I VEN E NRR R+LLF++ L + ISG+ILF ETLYQK G V ++++ G++PG
Sbjct: 77 INVENVESNRRALRELLFTTPGAL-QYISGIILFEETLYQKTASGKLFVDVMKEAGVLPG 135
Query: 467 IKVDKGVVPLFGS 505
IKVDKG V L G+
Sbjct: 136 IKVDKGTVELAGT 148
Score = 50.4 bits (115), Expect = 1e-06
Identities = 27/51 (52%), Positives = 29/51 (56%), Gaps = 1/51 (1%)
Frame = +1
Query: 511 ECTTQGLDDLASACAQYKKDGCHFAKWRCVLKIGRT-PLVQAXQENANVLA 660
E TT GLD L C +Y + G FAKWR VLKIG P A ENA LA
Sbjct: 151 ETTTTGLDGLGDRCKKYYEAGARFAKWRAVLKIGNNEPSELAIHENAYGLA 201
>At2g29980.2 68415.m03647 omega-3 fatty acid desaturase, endoplasmic
reticulum (FAD3) identical to SP:48623
Length = 288
Score = 30.7 bits (66), Expect = 0.91
Identities = 15/37 (40%), Positives = 20/37 (54%)
Frame = +1
Query: 265 NGQAFAGHRRGEHRGEPSSLSPTAIQL*RCALREHLW 375
NG AG R+ E R +PS+ P I R A+ +H W
Sbjct: 12 NGDPGAGDRKKEERFDPSAQPPFKIGDIRAAIPKHCW 48
>At2g29980.1 68415.m03646 omega-3 fatty acid desaturase, endoplasmic
reticulum (FAD3) identical to SP:48623
Length = 386
Score = 30.7 bits (66), Expect = 0.91
Identities = 15/37 (40%), Positives = 20/37 (54%)
Frame = +1
Query: 265 NGQAFAGHRRGEHRGEPSSLSPTAIQL*RCALREHLW 375
NG AG R+ E R +PS+ P I R A+ +H W
Sbjct: 12 NGDPGAGDRKKEERFDPSAQPPFKIGDIRAAIPKHCW 48
>At1g15550.1 68414.m01870 gibberellin 3-beta-dioxygenase /
gibberellin 3 beta-hydroxylase (GA4) identical to
gibberellin 3 beta-hydroxylase [GI:2160454]
Length = 358
Score = 28.7 bits (61), Expect = 3.7
Identities = 12/39 (30%), Positives = 20/39 (51%)
Frame = +3
Query: 180 ELQEELKKIAQAIVAPAKGILAADEPPVQWASVCRTSAW 296
E +E +KK+A ++ A L E ++WAS+ W
Sbjct: 169 EYEEHMKKLASKLMWLALNSLGVSEEDIEWASLSSDLNW 207
>At5g19460.1 68418.m02319 MutT/nudix family protein similar to
SP|P41888 Thiamine pyrophosphokinase (EC 2.7.6.2) (TPK)
(Thiamine kinase) {Schizosaccharomyces pombe}; contains
Pfam profile PF00293: NUDIX domain
Length = 374
Score = 27.9 bits (59), Expect = 6.4
Identities = 19/61 (31%), Positives = 30/61 (49%), Gaps = 4/61 (6%)
Frame = +2
Query: 302 TEENRRRYRQLLFSSDAVLSENISGVILFHETLYQKADDGT----PLVSLLEKKGIIPGI 469
TE R + FS + + + G + + L QK +D T ++ +L KGIIPGI
Sbjct: 116 TEYLREFHDIFTFSQNGSCPDRVDGYVTLNLML-QKPEDRTRAVADVIKILGDKGIIPGI 174
Query: 470 K 472
+
Sbjct: 175 R 175
>At3g27925.1 68416.m03484 DegP protease, putative SP:022609; almost
identical to DegP protease precursor GB:AF028842 from
[Arabidopsis thaliana] (J. Biol. Chem. 273 (12),
7094-7098 (1998))
Length = 439
Score = 27.5 bits (58), Expect = 8.5
Identities = 21/52 (40%), Positives = 27/52 (51%), Gaps = 3/52 (5%)
Frame = -3
Query: 289 DVLQTLAHCTGGSSAARIPFAGAT---IA*AIFLSSSCSSGVGY*K*VDIVG 143
DV+QT A G+S + + T I AI+ S SSGVG+ VD VG
Sbjct: 269 DVIQTDAAINPGNSGGPLLDSSGTLIGINTAIYSPSGASSGVGFSIPVDTVG 320
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 15,311,068
Number of Sequences: 28952
Number of extensions: 332671
Number of successful extensions: 999
Number of sequences better than 10.0: 15
Number of HSP's better than 10.0 without gapping: 928
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 986
length of database: 12,070,560
effective HSP length: 78
effective length of database: 9,812,304
effective search space used: 1403159472
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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